FastQCFastQC Report
Fri 10 Feb 2017
SRR2031862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023755
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80330.2656630580189202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64010.21169043126840634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37040.12249669698768584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42350.049.799291
GTATCAA75700.040.221271
TCAACGC85900.034.7264254
ATCAACG86750.034.3861663
CAACGCA87050.034.322235
AACGCAG90500.033.06636
TATCAAC92400.032.746212
ACGCAGA99500.030.1231167
CGCAGAG99950.029.8449238
GCAGAGT117350.025.0958679
AGAGTAC100050.022.45627210-11
GTACATG62700.022.0454541
TACATGG62050.021.8170832
GAGTACT82350.021.8032812-13
CAGAGTA113100.021.35610210-11
TAACCCG1353.7979207E-421.111115
ACATGGG62150.020.8648433
CATGGGG37950.019.5256924
GTACTTT91450.019.34800314-15
TATACTG17900.018.8407825
Produced by FastQC (version 0.11.5)