FastQCFastQC Report
Fri 10 Feb 2017
SRR2031862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023755
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94030.31097096160237847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76020.25140925769448913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46910.15513823044525765No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT36220.11978483706517228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.051.8809551
GTATCAA79800.041.56211
TCAACGC91500.035.2478684
CAACGCA92550.034.8992965
ATCAACG92900.034.8700683
AACGCAG96350.033.6707766
TATCAAC100400.032.454472
ACGCAGA106250.030.3993387
CGCAGAG105800.030.3490588
GCAGAGT124450.025.4192929
AGAGTAC108300.022.60915610-11
GAGTACT92500.022.59410312-13
GTACATG58550.022.3497891
CAGAGTA122300.021.55513210-11
TACATGG59700.021.1636942
ACATGGG57450.020.3389843
GTACTTT103350.019.9923114-15
AGTACTT100950.019.55009512-13
GTATAGC12850.018.885771
CGTAATA1901.8672583E-417.4996192
Produced by FastQC (version 0.11.5)