##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031860_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50521 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78982997169494 34.0 31.0 34.0 30.0 34.0 2 31.86726311830724 34.0 31.0 34.0 30.0 34.0 3 31.883434611349735 34.0 31.0 34.0 30.0 34.0 4 35.022940955246334 37.0 35.0 37.0 33.0 37.0 5 35.122582688387006 37.0 35.0 37.0 33.0 37.0 6 35.27491538172245 37.0 37.0 37.0 33.0 37.0 7 35.319154411037 37.0 37.0 37.0 35.0 37.0 8 35.31206824884701 37.0 37.0 37.0 35.0 37.0 9 37.03360978602957 39.0 39.0 39.0 34.0 39.0 10-11 37.092070624096905 39.0 39.0 39.0 35.0 39.0 12-13 37.082747768254784 39.0 39.0 39.0 35.0 39.0 14-15 38.51964529601551 41.0 39.0 41.0 34.5 41.0 16-17 38.38814552364363 41.0 39.0 41.0 34.5 41.0 18-19 38.29772767759941 41.0 39.0 41.0 35.0 41.0 20-21 38.22925120247026 41.0 39.0 41.0 34.5 41.0 22-23 38.00627461847549 41.0 38.0 41.0 34.0 41.0 24-25 37.91616357554284 41.0 38.0 41.0 34.0 41.0 26-27 37.681805585796 40.5 37.0 41.0 34.0 41.0 28-29 37.433354446665746 40.0 35.5 41.0 33.0 41.0 30-31 37.138615625185565 40.0 35.0 41.0 33.0 41.0 32-33 36.85422893450249 40.0 35.0 41.0 33.0 41.0 34-35 36.56347855347282 40.0 35.0 41.0 31.5 41.0 36-37 36.323261613982304 40.0 35.0 41.0 30.0 41.0 38-39 35.986203756853584 39.5 35.0 41.0 28.5 41.0 40-41 35.8384434195681 39.0 35.0 41.0 27.5 41.0 42-43 35.54766334791473 39.0 35.0 41.0 24.5 41.0 44-45 35.34262979750994 40.0 35.0 41.0 21.0 41.0 46-47 34.965489598384835 40.0 35.0 41.0 16.5 41.0 48-49 34.5465944854615 39.0 35.0 41.0 12.5 41.0 50-51 33.46017497674235 38.0 34.0 40.0 10.0 40.5 52-53 33.470537004414005 38.5 34.5 39.5 10.0 40.5 54-55 34.05725341937016 39.0 35.0 41.0 10.0 41.0 56-57 33.9799588290018 39.0 35.0 41.0 10.0 41.0 58-59 33.82040141723244 39.0 35.0 41.0 10.0 41.0 60-61 33.58587518061796 39.0 35.0 41.0 8.0 41.0 62-63 33.26239583539518 38.0 34.5 41.0 8.0 41.0 64-65 32.93436392787157 37.0 34.0 40.5 8.0 41.0 66-67 32.59813740820648 37.0 33.5 40.0 8.0 41.0 68-69 32.1988183131767 36.0 33.0 39.0 7.0 41.0 70-71 31.799538805645177 35.5 33.0 39.0 7.0 41.0 72-73 31.327200570059976 35.0 32.5 38.5 7.0 41.0 74-75 30.93744185586192 35.0 32.0 37.0 7.0 39.5 76-77 30.582579521387146 35.0 31.0 37.0 7.0 39.0 78-79 30.18623938560203 35.0 30.5 36.0 7.0 39.0 80-81 29.80456641792522 35.0 30.0 36.0 7.0 37.5 82-83 29.481878822667802 35.0 29.0 35.5 7.0 37.0 84-85 29.17725302349518 35.0 29.0 35.0 7.0 37.0 86-87 28.91796480671404 35.0 28.0 35.0 7.0 36.0 88-89 28.67122582688387 35.0 26.5 35.0 7.0 36.0 90-91 28.459125908038242 35.0 26.0 35.0 7.0 36.0 92-93 28.293373052789928 35.0 25.0 35.0 7.0 36.0 94-95 28.047613863541894 35.0 24.0 35.0 7.0 35.0 96-97 27.89077809227846 35.0 23.5 35.0 7.0 35.0 98-99 27.749569485956336 35.0 23.0 35.0 7.0 35.0 100-101 26.554531778864234 33.0 17.5 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.231532423184035 1101 2 -0.3468100196388839 1101 3 -0.10300828042003474 1101 4 -0.7506336976171255 1101 5 -1.7619888302731255 1101 6 -0.45511120934504135 1101 7 0.21637182448478853 1101 8 0.20207193306294613 1101 9 -0.6533537008019081 1101 10-11 0.03671566367126644 1101 12-13 0.052855678838660936 1101 14-15 0.33436402283299316 1101 16-17 0.36028343775682714 1101 18-19 0.7734364474652509 1101 20-21 0.7355757201704662 1101 22-23 0.826398389710107 1101 24-25 0.3911643141910446 1101 26-27 0.34242463912596577 1101 28-29 0.48366120415276015 1101 30-31 1.0003640665385518 1101 32-33 1.2310920812190815 1101 34-35 1.4556533318659959 1101 36-37 1.4764977554413221 1101 38-39 0.6890895740147798 1101 40-41 0.5951938571131805 1101 42-43 1.4269264115368259 1101 44-45 1.6400045560615624 1101 46-47 2.372636713471607 1101 48-49 2.1054678920677716 1101 50-51 2.1564990962496964 1101 52-53 2.8242122270213272 1101 54-55 1.9786827423380942 1101 56-57 1.296018793423329 1101 58-59 1.7863132924212621 1101 60-61 1.497895016011121 1101 62-63 2.250361759908067 1101 64-65 1.6223364671568063 1101 66-67 2.21852569407811 1101 68-69 2.084220873035953 1101 70-71 1.4106454585992445 1101 72-73 1.7243511216510257 1101 74-75 1.2171881610946222 1101 76-77 1.6308963365825413 1101 78-79 1.6844281331704778 1101 80-81 1.4045628871682823 1101 82-83 0.11318823997514826 1101 84-85 -0.28633463978061613 1101 86-87 0.4829120043121975 1101 88-89 -0.020031298491783645 1101 90-91 0.6249874856376039 1101 92-93 0.5071077497635876 1101 94-95 -0.3882710380859784 1101 96-97 0.864014909351507 1101 98-99 1.372294898563883 1101 100-101 0.6692695692992423 1102 1 -0.3975555892072009 1102 2 -0.5923698651987301 1102 3 0.10008052266876888 1102 4 0.7204087734253477 1102 5 0.2465053782210873 1102 6 -0.041983796217621716 1102 7 0.24108224919520893 1102 8 0.22678235777336653 1102 9 0.0037891563409502282 1102 10-11 0.2610399879955878 1102 12-13 0.14050046648344505 1102 14-15 -0.8733579848890187 1102 16-17 -0.34048876301536524 1102 18-19 0.4556758297046244 1102 20-21 0.1703247549194984 1102 22-23 0.6823829456946697 1102 24-25 0.7683843914111179 1102 26-27 0.2486022453035659 1102 28-29 0.6902249107164664 1102 30-31 1.2613679275424161 1102 32-33 1.2940264441534381 1102 34-35 1.0765027527154203 1102 36-37 0.5375016164451907 1102 38-39 0.5249969099221161 1102 40-41 0.44654520846453494 1102 42-43 0.6265403111507268 1102 44-45 0.5790006950576938 1102 46-47 0.7780421188770106 1102 48-49 1.0232285098283924 1102 50-51 1.1862288259794198 1102 52-53 1.6674554702645707 1102 54-55 1.1593777230330744 1102 56-57 0.7489145463190852 1102 58-59 0.7492476553556244 1102 60-61 1.0592849774010844 1102 62-63 1.0183154278617295 1102 64-65 0.9142283590486997 1102 66-67 1.0926569682093898 1102 68-69 1.5537189425340259 1102 70-71 1.0199118678656554 1102 72-73 1.0359341332340364 1102 74-75 1.2913194352258905 1102 76-77 0.5239465296327346 1102 78-79 -0.20205835331600497 1102 80-81 1.6354509180563142 1102 82-83 0.12361295039985976 1102 84-85 -0.7253307787767547 1102 86-87 -0.7502926288924385 1102 88-89 -1.2092204876809731 1102 90-91 -2.2757847151345985 1102 92-93 -1.6786065359506956 1102 94-95 -0.5535220033369406 1102 96-97 -0.49583065049405306 1102 98-99 -1.3462379199689387 1102 100-101 -1.5936647936351207 1103 1 0.8720390053873892 1103 2 0.6853328375039744 1103 3 0.8041345767228201 1103 4 1.138652016668587 1103 5 0.6451540268697329 1103 6 0.5877459335121031 1103 7 1.173514681627644 1103 8 0.5842147902057988 1103 9 0.8287891563409531 1103 10-11 1.0367156636712593 1103 12-13 0.7992842502672275 1103 14-15 0.8290068799758501 1103 16-17 0.6584977234711218 1103 18-19 0.9698650188938203 1103 20-21 1.3534328630276136 1103 22-23 1.7746126754243932 1103 24-25 1.4197357427624695 1103 26-27 2.131710353411677 1103 28-29 1.9425897755813324 1103 30-31 2.066435495109978 1103 32-33 1.8328777955047926 1103 34-35 2.228867617580285 1103 36-37 2.2800691840127527 1103 38-39 2.3783752883004894 1103 40-41 2.5451938571131834 1103 42-43 1.557283554393969 1103 44-45 2.2239331274901346 1103 46-47 1.19763671347161 1103 48-49 -0.578460679360802 1103 50-51 -1.2060009037503008 1103 52-53 -0.7382877729786657 1103 54-55 -1.1159601148047642 1103 56-57 -0.9004097780052405 1103 58-59 -0.6369009932930183 1103 60-61 0.12110930172540435 1103 62-63 0.2450046170509168 1103 64-65 0.4223364671568035 1103 66-67 -1.0850457344933133 1103 68-69 -0.805064841249763 1103 70-71 -0.23042596997218467 1103 72-73 -0.8488631640632534 1103 74-75 -0.3863832674768126 1103 76-77 -0.6351750919888879 1103 78-79 -0.3155718668295222 1103 80-81 0.03849145859685166 1103 82-83 0.13104538283229417 1103 84-85 0.2422367887908088 1103 86-87 0.5561262900264836 1103 88-89 0.8799687015082149 1103 90-91 0.7482017713518907 1103 92-93 0.8088934640493015 1103 94-95 0.6795861047711647 1103 96-97 0.8318720522086487 1103 98-99 1.30443775570674 1103 100-101 1.8174838550135277 1104 1 -0.315156116563827 1104 2 -0.23113057713017326 1104 3 0.373646771844772 1104 4 -0.3778113979655586 1104 5 -0.226797192642465 1104 6 -0.4098150420976481 1104 7 -0.3996560500796704 1104 8 -0.584687648818587 1104 9 -0.3864547460980745 1104 10-11 -0.41694287291410603 1104 12-13 -0.10010599363521777 1104 14-15 -0.13044433953634638 1104 16-17 0.06063186981258184 1104 18-19 0.23023087255235453 1104 20-21 0.16867676546663546 1104 22-23 -0.5756922026243814 1104 24-25 -0.09977645235947818 1104 26-27 -0.4969481831736928 1104 28-29 -0.3473492488089178 1104 30-31 0.24356964145144389 1104 32-33 -0.025353911812281638 1104 34-35 -0.5098518946148403 1104 36-37 -0.7607844745238239 1104 38-39 -0.7429661751141339 1104 40-41 -0.22187931361852264 1104 42-43 -0.3131432748743279 1104 44-45 -0.610518092022069 1104 46-47 0.5555635427399039 1104 48-49 0.5535515157611499 1104 50-51 0.5418649499082235 1104 52-53 0.6681146660457244 1104 54-55 0.3581252510488895 1104 56-57 0.522151197604515 1104 58-59 0.5762087628045407 1104 60-61 0.651901984652234 1104 62-63 0.8050655926606751 1104 64-65 0.8516047598397307 1104 66-67 0.658246948433515 1104 68-69 0.9650571099697487 1104 70-71 1.2796350056375694 1104 72-73 1.6166856164245473 1104 74-75 2.0032508788646517 1104 76-77 2.1096419811818485 1104 78-79 1.7966232551217018 1104 80-81 1.8223329220114906 1104 82-83 1.6901917242957047 1104 84-85 1.6227245936688597 1104 86-87 1.6076506802703818 1104 88-89 1.6263101649228524 1104 90-91 1.6253359176933522 1104 92-93 2.4433446835614987 1104 94-95 2.6399519584297018 1104 96-97 2.438579369281822 1104 98-99 1.8172426337555194 1104 100-101 2.1037643428184047 1105 1 0.6288571872055719 1105 2 0.4512419284130651 1105 3 0.813225485813728 1105 4 0.8272883803049567 1105 5 0.8269722086879128 1105 6 0.528655024421198 1105 7 0.7189692270821908 1105 8 0.8410329720239815 1105 9 0.8901527927045834 1105 10-11 0.561715663671265 1105 12-13 0.4242842502672275 1105 14-15 0.3551432436122113 1105 16-17 0.49827045074384557 1105 18-19 0.7130468370756304 1105 20-21 0.7102510448457906 1105 22-23 0.787112675424396 1105 24-25 0.8129175609442854 1105 26-27 0.17489217159349124 1105 28-29 0.21418068467223605 1105 30-31 0.5675718587463408 1105 32-33 0.8612868864138861 1105 34-35 0.7481857993984633 1105 36-37 0.570978274921849 1105 38-39 -0.18753380260859842 1105 40-41 -0.17753341561409286 1105 42-43 -0.6415800819696713 1105 44-45 -1.6476577816007776 1105 46-47 -0.5023632865283929 1105 48-49 0.7317665933664657 1105 50-51 1.3655900053406 1105 52-53 0.8446667724758754 1105 54-55 1.1738126124679624 1105 56-57 0.4359538583584026 1105 58-59 0.4642353703433386 1105 60-61 -0.2754816073654993 1105 62-63 1.173413707960016 1105 64-65 1.704154648974992 1105 66-67 1.1626815382339544 1105 68-69 1.6562987951138766 1105 70-71 0.38661948457327 1105 72-73 0.8522731995731121 1105 74-75 0.7704349143413687 1105 76-77 0.6409612716474768 1105 78-79 0.954882678625026 1105 80-81 0.8464460040514012 1105 82-83 0.40831811010501795 1105 84-85 0.5240549706089901 1105 86-87 -0.5586464372462459 1105 88-89 -0.6563949348554203 1105 90-91 0.3243381349882526 1105 92-93 -0.5990610814052424 1105 94-95 -0.024959349774288597 1105 96-97 0.22959932493592206 1105 98-99 0.01125593752491838 1105 100-101 -1.2291070540773816 1106 1 0.22029481934088224 1106 2 0.3428909770388522 1106 3 0.40994853021119226 1106 4 0.7543496910871923 1106 5 0.17422379431159385 1106 6 0.7342575614190778 1106 7 0.7897937513950879 1106 8 1.14758688322906 1106 9 1.4223938075037381 1106 10-11 1.2506691520433577 1106 12-13 1.1103307618951348 1106 14-15 1.0263906009060833 1106 16-17 1.2971605141687945 1106 18-19 0.9820743212193932 1106 20-21 1.2668049560508692 1106 22-23 1.0981591870523033 1106 24-25 0.8290380683438627 1106 26-27 0.8450824464349367 1106 28-29 0.9504386127906272 1106 30-31 0.9452145648774248 1106 32-33 0.8779359350396803 1106 34-35 1.173925757115171 1106 36-37 1.616697090989497 1106 38-39 2.124305520858634 1106 40-41 1.5486822292062001 1106 42-43 0.590713786952108 1106 44-45 -0.767636639951732 1106 46-47 -0.6273632865283929 1106 48-49 -0.11944905145382023 1106 50-51 -0.5783846246805382 1106 52-53 -0.7153226566995983 1106 54-55 -1.7598554636419692 1106 56-57 -1.0986655919587207 1106 58-59 -0.8994009932930211 1106 60-61 -0.779181395949017 1106 62-63 -0.5366814294607067 1106 64-65 -1.245105393308311 1106 66-67 -1.8658596879816827 1106 68-69 -2.2068090272962735 1106 70-71 -1.6900189932279979 1106 72-73 -1.4008980477841853 1106 74-75 -2.0392902442209966 1106 76-77 -2.179070440826095 1106 78-79 -1.6574323319457989 1106 80-81 -0.9170317972170992 1106 82-83 -1.1416290357723575 1106 84-85 -2.239158560046402 1106 86-87 -1.5755597564851485 1106 88-89 -2.345612693840618 1106 90-91 -1.7892982286481107 1106 92-93 -1.6669786289739505 1106 94-95 -1.31750691848465 1106 96-97 -2.4210349245355367 1106 98-99 -2.263585500107215 1106 100-101 -2.5612952147539154 1107 1 -0.332372759318492 1107 2 -0.2622161821038702 1107 3 -0.3556693448458077 1107 4 0.5714951539234931 1107 5 -0.49700283587536376 1107 6 -1.3318619096251538 1107 7 -0.6794264948429429 1107 8 0.149410868637176 1107 9 -0.6432696671884628 1107 10-11 -0.7789706108385417 1107 12-13 -0.8796373183602171 1107 14-15 -0.7437872376712065 1107 16-17 -0.6089042373131974 1107 18-19 -0.46297811836108593 1107 20-21 -0.9568612546194473 1107 22-23 -0.4898481088893334 1107 24-25 -0.8606564141002764 1107 26-27 -1.0746621956079352 1107 28-29 -0.6507925773598444 1107 30-31 -0.7396919558704056 1107 32-33 -1.7565829888089297 1107 34-35 -1.49000493143933 1107 36-37 -1.4635582669676381 1107 38-39 -0.7534874567975436 1107 40-41 -1.1204924173966262 1107 42-43 -0.2576674259981857 1107 44-45 0.05064881376463859 1107 46-47 -1.3528534826068253 1107 48-49 -1.1071371499490326 1107 50-51 -0.7297754135542291 1107 52-53 -1.1051995376845554 1107 54-55 -2.2568914873537835 1107 56-57 -1.918547032907199 1107 58-59 -2.4974402089792953 1107 60-61 -2.5183514825883186 1107 62-63 -2.449358128047116 1107 64-65 -2.5168792191177047 1107 66-67 -1.2963202442972346 1107 68-69 -1.2594766059556477 1107 70-71 -1.337533813109438 1107 72-73 -1.1045004189652161 1107 74-75 -0.4604028753199465 1107 76-77 -0.6466946998320218 1107 78-79 -0.7743953962412853 1107 80-81 -2.069106580618829 1107 82-83 -1.8066997152069213 1107 84-85 -1.0499200739542864 1107 86-87 -1.880393317816658 1107 88-89 -1.363168553393745 1107 90-91 -1.2991021502167364 1107 92-93 -2.1001751634016763 1107 94-95 -2.337570757973932 1107 96-97 -2.2833240262227257 1107 98-99 -1.8960524403716938 1107 100-101 -1.405800458711962 1108 1 -0.2710461009955871 1108 2 0.0677796460146105 1108 3 0.1115813852334604 1108 4 0.006205208157950892 1108 5 0.5760050906995176 1108 6 0.3685969973418892 1108 7 -0.02861297794682116 1108 8 0.020916917865378082 1108 9 0.4718742627239294 1108 10-11 0.11756672750105679 1108 12-13 -0.12890723909447388 1108 14-15 0.012783475720532067 1108 16-17 0.2842955958115496 1108 18-19 0.6874182103831785 1108 20-21 0.12949669281484688 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80-81 0.14320457335095327 2312 82-83 0.5314552188978645 2312 84-85 0.39141711665966383 2312 86-87 0.2178066178953344 2312 88-89 0.7832473900328054 2312 90-91 0.6123411156141856 2312 92-93 0.9033606771640557 2312 94-95 0.31688118673837806 2312 96-97 1.0101507407332413 2312 98-99 1.2966508704608373 2312 100-101 0.5549838550135284 2313 1 -0.802960994612608 2313 2 -1.1154736141089323 2313 3 -1.7490912297287906 2313 4 -2.53634798333141 2313 5 -1.7822653279689789 2313 6 -1.215479872939511 2313 7 -1.084549834501388 2313 8 -3.01820456463291 2313 9 -2.334920521078402 2313 10-11 -1.4745746589093756 2313 12-13 -1.1321673626360038 2313 14-15 -2.0326866684112517 2313 16-17 -2.0072280829804967 2313 18-19 -1.3470704649771506 2313 20-21 -2.5118897176175494 2313 22-23 -3.3620808729627 2313 24-25 -2.235102966914951 2313 26-27 -1.6594186788463858 2313 28-29 -1.2916844179670548 2313 30-31 -2.0097741823093784 2313 32-33 -1.5401060754629512 2313 34-35 -2.2763743179035885 2313 36-37 -2.290092106309821 2313 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0.0059996335990391 2315 16-17 0.31266439013779035 2315 18-19 0.3187780623720826 2315 20-21 0.16973721085369675 2315 22-23 0.028054704409903763 2315 24-25 -0.010699039846223002 2315 26-27 -0.24473892195064195 2315 28-29 0.07682890601611092 2315 30-31 -0.018890591846535187 2315 32-33 -0.5357816247850664 2315 34-35 -1.5731251360429042 2315 36-37 -0.7789887870017367 2315 38-39 -0.06039282764153597 2315 40-41 -0.2236467225969605 2315 42-43 -0.3548541267654528 2315 44-45 -0.18421904642291764 2315 46-47 -0.24330531551390067 2315 48-49 -0.7239316938535509 2315 50-51 -0.3623414834604475 2315 52-53 -0.5395558889207024 2315 54-55 -0.577010839442444 2315 56-57 -1.3362793432226283 2315 58-59 -1.1095459208292553 2315 60-61 -0.5729124374050301 2315 62-63 -0.7272779916447334 2315 64-65 -0.6805620835678354 2315 66-67 -0.6172921113049057 2315 68-69 -0.7590503484961388 2315 70-71 -0.028070897508417403 2315 72-73 -0.8048414249328175 2315 74-75 -0.29544123849130344 2315 76-77 -0.1411533528584492 2315 78-79 -0.8894849103077789 2315 80-81 -1.3602404254611145 2315 82-83 -0.5961285302111854 2315 84-85 -1.0925458199048421 2315 86-87 -1.2444171882343866 2315 88-89 -1.6866979651584515 2315 90-91 -1.7168344605321693 2315 92-93 -1.6713601591391019 2315 94-95 -1.1483124459534757 2315 96-97 -1.4887801217043943 2315 98-99 -1.5328810848729724 2315 100-101 -1.4595088986096592 2316 1 -0.16407210572371866 2316 2 -0.042444940273806964 2316 3 -0.17919875661051066 2316 4 -0.036347983331410205 2316 5 0.20070958242529002 2316 6 -0.1733651775990097 2316 7 0.2290702371832012 2316 8 0.15921479020580165 2316 9 0.5454558230076145 2316 10-11 -0.2021732252176207 2316 12-13 -0.21460463862165824 2316 14-15 -1.16127089780192 2316 16-17 -1.0137244987511025 2316 18-19 -1.3481905366617397 2316 20-21 -0.7007338036390607 2316 22-23 -0.5462206579089397 2316 24-25 -0.27609759057085625 2316 26-27 -0.7196785354772075 2316 28-29 -1.3268546688631133 2316 30-31 -1.108564504890019 2316 32-33 -0.6671222044952074 2316 34-35 -0.09335460464194512 2316 36-37 0.19534696179052702 2316 38-39 -0.19384693392172636 2316 40-41 -1.488139476220148 2316 42-43 -0.17188311227269537 2316 44-45 0.011433127490128925 2316 46-47 0.2615256023604928 2316 48-49 0.1882059873058708 2316 50-51 0.5259435406941364 2316 52-53 0.590878893687993 2316 54-55 0.1340398851952358 2316 56-57 0.5079235553280981 2316 58-59 0.9478212289292003 2316 60-61 1.0572204128365215 2316 62-63 0.5061157281620368 2316 64-65 0.6195586893790264 2316 66-67 0.45939870995113097 2316 68-69 0.8602129365280078 2316 70-71 0.5987406966944882 2316 72-73 1.0303035026034166 2316 74-75 0.22056117696763167 2316 76-77 -0.17406398087777575 2316 78-79 0.27053924428158993 2316 80-81 0.7107136808190759 2316 82-83 0.10465649394340204 2316 84-85 0.4116812332352531 2316 86-87 0.2727929566931486 2316 88-89 0.5563575903971021 2316 90-91 0.9607017713518893 2316 92-93 0.4950045751604151 2316 94-95 0.4643083269933861 2316 96-97 0.46381649665309155 2316 98-99 0.8919377557067385 2316 100-101 1.4022060772357499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 132.0 8 298.0 9 243.0 10 189.0 11 210.0 12 245.0 13 245.0 14 209.0 15 218.0 16 339.0 17 590.0 18 617.0 19 816.0 20 994.0 21 973.0 22 840.0 23 640.0 24 552.0 25 512.0 26 516.0 27 491.0 28 526.0 29 593.0 30 612.0 31 797.0 32 908.0 33 1123.0 34 1736.0 35 4820.0 36 4226.0 37 7485.0 38 14146.0 39 3679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.81898616416935 23.15274836206726 14.754260604501098 17.27400486926229 2 16.47631677916114 26.305892599117197 37.92680271570238 19.29098790601928 3 18.259733576136654 30.575404287326062 35.1596365867659 16.00522554977138 4 10.43922329328398 24.858969537420084 40.03879574830268 24.66301142099325 5 9.101165851823994 41.80637754597098 36.19089091664852 12.901565685556502 6 21.10607470160923 41.89544941707409 18.44579481799648 18.552681063320204 7 18.756556679400646 42.000356287484415 24.223590190217926 15.019496842897013 8 22.31151402387126 35.19328596029374 22.982522119514655 19.51267789632034 9 24.009817699570476 19.10888541398626 25.334019516636648 31.547277369806615 10-11 21.030858454899942 29.31157340511866 29.869757130698126 19.787811009283267 12-13 23.66837552700857 31.282041131410697 29.28683121870113 15.762752122879595 14-15 20.370736921280262 32.2143267156232 30.272559925575504 17.14237643752103 16-17 15.389639951703252 35.509985946438114 30.82876427624156 18.27160982561707 18-19 15.404485263553768 34.233289127293595 34.252093188970925 16.110132420181706 20-21 17.27598424417569 32.36080045921498 34.30652599908949 16.056689297519846 22-23 17.173056748678768 32.99321074404703 33.510817283901744 16.32291522337246 24-25 16.862294887274597 33.85126976900695 33.42867322499555 15.857762118722906 26-27 16.31400803626215 33.01003543081095 34.86965816195246 15.806298370974448 28-29 16.12992616931573 33.47716791037391 34.67271035806892 15.720195562241443 30-31 17.105758001623087 32.297460461986105 34.49852536568952 16.09825617070129 32-33 16.07153460937036 32.85366481265217 35.501078759327804 15.573721818649672 34-35 16.188317729261097 33.36533322776667 34.431226618633836 16.015122424338394 36-37 17.13148987549732 32.31923358603353 33.76714633518735 16.782130203281802 38-39 16.01413273688169 33.86116664357396 33.71568258743889 16.40901803210546 40-41 15.833019932305378 33.83444508224302 34.380752558342074 15.951782427109517 42-43 15.681597751430099 33.706775400328574 33.505868846618235 17.105758001623087 44-45 16.216028978048733 32.63692325963461 33.77704320975436 17.3700045525623 46-47 17.051325191504525 33.19510698521407 32.6339541972645 17.119613626016907 48-49 16.159616793016767 32.729953880564516 33.45143603649967 17.658993289919046 50-51 15.147166524811464 32.815067001840816 33.85621820629046 18.181548267057263 52-53 16.436729280893093 33.37621978979038 32.89127293600681 17.295777993309713 54-55 15.760772747966193 32.18463609192217 33.2238079214584 18.830783238653233 56-57 16.483244591358048 32.354862334474774 32.88929356109341 18.27259951307377 58-59 17.020644880346786 31.871894855604598 32.779438253399576 18.328022010649036 60-61 17.02658300508699 32.36475920904178 32.761623879178956 17.847033906692268 62-63 16.179410542150787 32.247976089151045 33.02290136774806 18.549712000950098 64-65 16.00423586231468 32.93185012173156 32.49143920349953 18.572474812454224 66-67 16.545594901130222 32.10744047029948 31.84616298173037 19.50080164683993 68-69 16.506007402862178 31.46909206072722 32.149997030937634 19.87490350547297 70-71 17.431365174877776 32.00451297480256 31.557174244373627 19.006947605946042 72-73 17.888600779873716 30.536806476514716 32.29943983689951 19.27515290671206 74-75 16.814789889352944 32.26282140100156 30.881217711446723 20.04117099819877 76-77 17.19285049781279 31.655153302587042 31.202866134874608 19.94913006472556 78-79 17.374952989845806 32.137131094000516 30.854496150115796 19.633419766037886 80-81 18.42303200649235 30.943568021218898 30.867362087052907 19.766037885235843 82-83 17.84802359414897 31.98273985075513 31.126660200708617 19.042576354387283 84-85 18.318125136082024 31.445339561766396 30.6931770946735 19.54335820747808 86-87 18.426990756319153 31.894657667108728 30.331941172977572 19.346410403594543 88-89 19.189050097979056 31.552225807090124 29.561964331664058 19.696759763266762 90-91 19.701708200550268 30.603115536113695 30.26365273846519 19.431523524870844 92-93 20.47861285406069 30.65853803368896 30.525919914491006 18.336929197759346 94-95 20.46079847984007 30.086498683715686 30.32996179806417 19.122741038380077 96-97 20.11935630727816 29.918251816076484 30.71791928109103 19.244472595554324 98-99 20.05205756022248 30.021179311573405 29.77771619722492 20.149046930979196 100-101 21.092219077215415 29.43627402466301 29.898458066942457 19.57304883117911 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1970.0 1 1143.0 2 344.5 3 390.0 4 276.5 5 163.0 6 248.5 7 460.5 8 603.5 9 534.5 10 326.0 11 172.0 12 154.5 13 147.0 14 141.5 15 136.0 16 137.0 17 149.5 18 148.5 19 142.5 20 160.0 21 179.5 22 207.5 23 239.0 24 269.0 25 343.0 26 504.0 27 711.5 28 802.0 29 860.5 30 1071.0 31 1271.5 32 1385.0 33 1523.0 34 1797.0 35 2108.0 36 2277.0 37 2227.5 38 1921.0 39 1616.0 40 1427.5 41 1390.0 42 1319.5 43 1232.5 44 1181.0 45 1079.0 46 1075.5 47 1287.5 48 1957.5 49 2108.5 50 1575.5 51 1209.5 52 884.5 53 634.5 54 506.5 55 452.5 56 396.0 57 328.0 58 280.0 59 242.5 60 216.5 61 172.5 62 165.5 63 155.0 64 122.0 65 115.0 66 122.5 67 110.0 68 87.0 69 64.5 70 47.5 71 46.5 72 41.0 73 33.5 74 24.5 75 23.0 76 23.5 77 20.5 78 21.5 79 18.0 80 11.0 81 9.0 82 5.5 83 2.5 84 4.0 85 3.0 86 1.0 87 1.0 88 2.5 89 2.0 90 0.0 91 0.5 92 1.0 93 1.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 50521.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.0114011995012 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76635934337756 33.78595039686467 2 10.34405217000225 9.105124601650799 3 4.101641556105239 5.415569763068823 4 2.1857431976613446 3.8479048316541635 5 1.4796492017090173 3.256071732546862 6 1.0209129750393524 2.6959086320539973 7 0.7960422756914773 2.4524455177055087 8 0.47672588261749493 1.6785099265651908 9 0.368787946930515 1.4607786860909324 >10 2.2082302675961323 17.262128619781873 >50 0.17090173150438498 5.358167890580154 >100 0.062963795817405 6.0509491102709765 >500 0.013492241960872498 4.324934185784129 >1k 0.0044974139869575 3.3055561053819202 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1670 3.3055561053819202 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 1.8131074206765505 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 1.3004493181053423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 612 1.2113774470022367 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 461 0.9124918350784822 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 358 0.7086162189980404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 327 0.6472555966825676 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 316 0.6254824726351418 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 283 0.5601631004928643 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.5542249757526573 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 179 0.3543081094990202 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 141 0.27909186278973097 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.2533599889155005 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 127 0.2513806140020981 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 122 0.2414837394350864 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 121 0.23950436452168403 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.21971061538766057 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 103 0.2038756160804418 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 94 0.18606124185982068 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 92 0.18210249203301598 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 90 0.17814374220621126 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 89 0.17616436729280893 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 88 0.1741849923794066 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 87 0.17220561746600424 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 86 0.17022624255260188 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 85 0.16824686763919955 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 83 0.16428811781239483 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 80 0.1583499930721878 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 80 0.1583499930721878 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 78 0.15439124324538311 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 78 0.15439124324538311 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 77 0.15241186833198075 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.15241186833198075 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 77 0.15241186833198075 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 74 0.14647374359177373 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 74 0.14647374359177373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.142514993764969 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 70 0.13855624393816432 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 69 0.136576869024762 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 69 0.136576869024762 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 67 0.1326181191979573 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.1326181191979573 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 66 0.13063874428455494 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 64 0.12667999445775024 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62 0.12272124463094554 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 60 0.11876249480414086 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 60 0.11876249480414086 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.11876249480414086 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 57 0.11282437006393381 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 57 0.11282437006393381 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 56 0.11084499515053148 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 54 0.10688624532372677 No Hit ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG 53 0.10490687041032443 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.10490687041032443 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 51 0.10094812058351972 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 51 0.10094812058351972 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0019793749134023476 0.0 14-15 0.0 0.0 0.0 0.0019793749134023476 0.0 16-17 0.0 0.0 0.0 0.0019793749134023476 0.0 18-19 0.0 0.0 0.0 0.0019793749134023476 0.0 20-21 0.0 0.0 0.0 0.0019793749134023476 0.0 22-23 0.0 0.0 0.0 0.004948437283505869 0.0 24-25 0.0 0.0 0.0 0.02672156133093169 0.0 26-27 0.0 0.0 0.0 0.07224718433918569 0.0 28-29 0.0 0.0 0.0 0.1811128045763148 0.0 30-31 0.0 0.0 0.0 0.36915342134953777 0.0 32-33 0.0 0.0 0.0 0.5720393499732784 0.0 34-35 0.0 0.0 0.0 0.8313374636289859 0.0 36-37 0.0 0.0 0.0 1.1757486985609944 0.0 38-39 0.0 0.0 0.0 1.57657211852497 0.0 40-41 0.0 0.0 0.0 2.077353971615764 0.0 42-43 0.0 0.0 0.0 2.4593733299024167 0.0 44-45 0.0 0.0 0.0 2.7434136299756537 0.0 46-47 0.0 0.0 0.0 2.9858870568674414 0.0 48-49 0.0 0.0 0.0 3.2145048593654124 0.0 50-51 0.0 0.0 0.0 3.4708339106510167 0.0 52-53 0.0 0.0 0.0 3.690544526038677 0.0 54-55 0.0 0.0 0.0 3.982502325765523 0.0 56-57 0.0 0.0 0.0 4.374418558619189 0.0 58-59 0.0 0.0 0.0 4.6990360444171735 0.0 60-61 0.0 0.0 0.0 4.992973219057422 0.0 62-63 0.0 0.0 0.0 5.249302270343025 0.0 64-65 0.0 0.0 0.0 5.518497258565745 0.0 66-67 0.0 0.0 0.0 5.85202193147404 0.0 68-69 0.0 0.0 0.0 6.333999722887512 0.0 70-71 0.0 0.0 0.0 6.818946576671087 0.0 72-73 0.0 0.0 0.0 7.3038934304546625 0.0 74-75 0.0 0.0 0.0 7.7413352863165805 0.0 76-77 0.0 0.0 0.0 8.176797767265098 0.0 78-79 0.0 0.0 0.0 8.645909621741454 0.0 80-81 0.0 0.0 0.0 9.179351160903387 0.0 82-83 0.0 0.0 0.0 9.674194889253974 0.0 84-85 0.0 0.0 0.0 10.112626432572593 0.0 86-87 0.0 0.0 0.0 10.551057975891213 0.0 88-89 0.0 0.0 0.0 11.126066388234594 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 20 0.0020625773 71.25 1 GTATTAG 25 0.0049935943 57.0 1 TTAGAGG 25 0.0049935943 57.0 4 GGATATG 25 0.0049935943 57.0 7 ATATAGG 35 2.7624334E-4 54.28571 2 GGAAACC 35 2.7624334E-4 54.28571 9 CGGAATC 20 5.3647265E-4 47.500004 38-39 AATCAGC 20 5.3647265E-4 47.500004 40-41 ATCAGCG 20 5.3647265E-4 47.500004 42-43 GGCTTGG 20 5.3647265E-4 47.500004 30-31 AACGGGC 20 5.3647265E-4 47.500004 26-27 GGGAACG 20 5.3647265E-4 47.500004 24-25 ACGGAGA 20 5.3647265E-4 47.500004 62-63 GAACGGG 20 5.3647265E-4 47.500004 26-27 GCGGAAT 20 5.3647265E-4 47.500004 36-37 GCTTGGC 25 0.0016088454 38.0 32-33 TGGCCTA 25 0.0016088454 38.0 14-15 ACGGGCT 25 0.0016088454 38.0 28-29 GGGAAAC 50 0.0016012264 38.0 8 CGGGCTT 25 0.0016088454 38.0 28-29 >>END_MODULE