##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031860_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50521 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.71754319985749 34.0 31.0 34.0 31.0 34.0 2 32.82142079531284 34.0 31.0 34.0 31.0 34.0 3 32.83009045743354 34.0 33.0 34.0 31.0 34.0 4 36.236456127155044 37.0 35.0 37.0 35.0 37.0 5 36.28708853743988 37.0 37.0 37.0 35.0 37.0 6 36.40494051978385 37.0 37.0 37.0 35.0 37.0 7 36.37972328338711 37.0 37.0 37.0 35.0 37.0 8 36.42871281249381 37.0 37.0 37.0 35.0 37.0 9 38.192593179074045 39.0 39.0 39.0 37.0 39.0 10-11 38.188723501118346 39.0 39.0 39.0 37.0 39.0 12-13 38.22010649037034 39.0 39.0 39.0 37.0 39.0 14-15 39.65628154628768 41.0 40.0 41.0 37.0 41.0 16-17 39.572425328081394 41.0 39.0 41.0 37.0 41.0 18-19 39.5286217612478 41.0 39.5 41.0 35.5 41.0 20-21 39.49886185942479 41.0 39.0 41.0 35.0 41.0 22-23 39.3250925357772 41.0 39.0 41.0 35.0 41.0 24-25 39.14790879040399 41.0 38.5 41.0 35.0 41.0 26-27 38.91573800993646 41.0 38.0 41.0 35.0 41.0 28-29 38.73803962708577 40.5 37.5 41.0 35.0 41.0 30-31 38.47616832604264 40.0 36.5 41.0 35.0 41.0 32-33 37.83421745412799 40.0 35.0 41.0 34.5 41.0 34-35 37.753181845173295 40.0 35.0 41.0 35.0 41.0 36-37 37.55288889768611 40.0 35.0 41.0 34.0 41.0 38-39 37.23239840858257 40.0 35.0 41.0 33.0 41.0 40-41 37.10839057025791 40.0 35.0 41.0 33.0 41.0 42-43 36.829892519942206 40.0 35.0 41.0 33.0 41.0 44-45 36.42912848122563 40.0 35.0 41.0 31.5 41.0 46-47 36.00914471209992 40.0 35.0 41.0 29.0 41.0 48-49 35.6607153460937 39.5 35.0 41.0 24.0 41.0 50-51 35.37455711486312 40.0 35.0 41.0 21.0 41.0 52-53 35.45104016151699 40.0 35.0 41.0 20.0 41.0 54-55 35.297975099463585 39.5 35.0 41.0 19.0 41.0 56-57 35.07430573424912 39.0 35.0 41.0 18.0 41.0 58-59 34.86298766849429 39.0 35.0 41.0 15.0 41.0 60-61 34.46275806100434 39.0 35.0 41.0 13.0 41.0 62-63 34.1034124423507 38.0 35.0 41.0 10.0 41.0 64-65 33.72954810870727 37.0 35.0 40.5 10.0 41.0 66-67 33.21176342511035 37.0 34.5 40.0 10.0 41.0 68-69 32.56307278161556 36.0 33.5 39.0 9.0 41.0 70-71 31.994042081510656 35.5 33.0 39.0 8.0 41.0 72-73 31.4627976485026 35.0 33.0 38.5 7.0 40.5 74-75 31.092327942835652 35.0 32.5 37.0 7.0 39.5 76-77 29.87118228063578 34.5 30.0 36.0 7.0 39.0 78-79 30.489044159854316 35.0 31.5 36.0 7.0 39.0 80-81 30.314740404980107 35.0 32.0 36.0 7.0 37.0 82-83 30.0410423388294 35.0 31.0 35.5 7.0 37.0 84-85 29.824340373310108 35.0 31.0 35.0 7.0 37.0 86-87 29.647997862275094 35.0 31.0 35.0 7.0 36.0 88-89 29.48526355376972 35.0 31.0 35.0 7.0 36.0 90-91 29.300518596227313 35.0 30.0 35.0 7.0 36.0 92-93 29.1519863027256 35.0 29.5 35.0 7.0 36.0 94-95 29.045881910492668 35.0 29.0 35.0 7.0 35.0 96-97 28.94033174323549 35.0 29.0 35.0 7.0 35.0 98-99 28.878872152174345 35.0 29.0 35.0 7.0 35.0 100-101 27.968518042002337 34.0 26.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3835734796973185 1101 2 0.4638990265890399 1101 3 0.3611199301149881 1101 4 0.1951634364173387 1101 5 0.3228855550945866 1101 6 0.2938067833560325 1101 7 0.12158832061015801 1101 8 -0.050237653619163325 1101 9 0.12208334192107628 1101 10-11 -0.21924193458394825 1101 12-13 0.12643319652584495 1101 14-15 0.3655793774360987 1101 16-17 0.015181481242656503 1101 18-19 0.4823653871374418 1101 20-21 0.7065811320726496 1101 22-23 0.5415995365215096 1101 24-25 0.1230325426219494 1101 26-27 0.5484651790242836 1101 28-29 0.6198147775773251 1101 30-31 0.4263925492797327 1101 32-33 0.2636706586562809 1101 34-35 -0.14915557047493166 1101 36-37 -0.1425958367656719 1101 38-39 0.31186183694097735 1101 40-41 0.7338313365915425 1101 42-43 0.5548860740837824 1101 44-45 -0.052781723344430986 1101 46-47 -0.23016654141920867 1101 48-49 -0.1625504362591812 1101 50-51 0.22054595937019172 1101 52-53 0.5068432114339387 1101 54-55 -0.02932961341264928 1101 56-57 0.02123350598684226 1101 58-59 0.7142913705379499 1101 60-61 0.2139415741062578 1101 62-63 -0.5854911349742835 1101 64-65 -0.5026468051852575 1101 66-67 -0.5365459002332003 1101 68-69 -0.0789096877855755 1101 70-71 0.33687096006247685 1101 72-73 -0.12535416741555494 1101 74-75 0.3930255505570024 1101 76-77 -0.1477173894205741 1101 78-79 -0.3582855517187049 1101 80-81 -0.126839800405115 1101 82-83 0.07021826203840575 1101 84-85 -0.3987059836878615 1101 86-87 0.20775741783215906 1101 88-89 -0.07447041448932268 1101 90-91 0.04168504488290026 1101 92-93 0.16273787533920014 1101 94-95 0.24503127835374983 1101 96-97 -0.2973836600590083 1101 98-99 0.2038662419268391 1101 100-101 -0.5732066886961995 1102 1 0.024500120623955013 1102 2 -0.014093251403238582 1102 3 -0.056640687645625576 1102 4 0.2785611198150235 1102 5 0.12829096049998867 1102 6 0.2551967447459944 1102 7 0.3100053090271473 1102 8 -0.046376649758158806 1102 9 0.591581411419142 1102 10-11 0.349870034528017 1102 12-13 0.4171667872594398 1102 14-15 0.2281276399843577 1102 16-17 0.1402780063391802 1102 18-19 0.43796384273590405 1102 20-21 0.09731472280623876 1102 22-23 0.5319470268689983 1102 24-25 0.3469707665601689 1102 26-27 -0.2646622341031346 1102 28-29 -0.25624699848444976 1102 30-31 -0.5037232808360912 1102 32-33 -0.02629073130511017 1102 34-35 -0.5757964971158529 1102 36-37 -0.2754143695842046 1102 38-39 1.0076147326938738 1102 40-41 0.4936768964371083 1102 42-43 0.47380499300270174 1102 44-45 0.06652329596058593 1102 46-47 0.058250446997782035 1102 48-49 -0.14401761772636235 1102 50-51 0.1908162296404683 1102 52-53 0.7145652191559506 1102 54-55 0.1756896916066566 1102 56-57 0.6521215368748727 1102 58-59 0.8467238029703879 1102 60-61 1.0205052806699584 1102 62-63 1.4203003708172233 1102 64-65 1.099669797131341 1102 66-67 0.7167359530486479 1102 68-69 1.0253374164615323 1102 70-71 0.7310794542709758 1102 72-73 0.8364218943605088 1102 74-75 0.6354965930280478 1102 76-77 -0.44617298787617443 1102 78-79 0.14518935175619774 1102 80-81 0.6762490026836865 1102 82-83 0.9845039763241203 1102 84-85 0.42793494295306544 1102 86-87 0.7235875336622755 1102 88-89 0.2251434851245797 1102 90-91 0.45635685955471317 1102 92-93 0.686289998891322 1102 94-95 0.7805525138749871 1102 96-97 0.3705700078946599 1102 98-99 0.5362986743592728 1102 100-101 -0.8411603566498655 1103 1 0.5157163368401712 1103 2 0.4960418837318983 1103 3 0.7075485015435632 1103 4 0.4130205792744803 1103 5 0.24788555509458376 1103 6 0.37952106907031435 1103 7 0.33230260632444697 1103 8 0.2461909178094075 1103 9 0.5827976276353581 1103 10-11 0.45932949398747525 1103 12-13 0.21750462509727697 1103 14-15 0.07272223457895421 1103 16-17 0.21696719552837607 1103 18-19 0.7234368157088724 1103 20-21 0.7351525606440745 1103 22-23 0.7130281079500804 1103 24-25 0.8658896854790896 1103 26-27 0.893108036167142 1103 28-29 0.45017192043446386 1103 30-31 0.35674969213688 1103 32-33 1.1118849443705727 1103 34-35 0.3829872866679267 1103 36-37 0.9002613060914726 1103 38-39 1.4082904083695524 1103 40-41 0.7534741937344123 1103 42-43 0.5298860740837839 1103 44-45 0.44186113379842595 1103 46-47 0.2930477442950803 1103 48-49 -0.5696932934020396 1103 50-51 -0.3544540406298111 1103 52-53 -1.000299645708921 1103 54-55 -0.9239724705555048 1103 56-57 -1.232337922584584 1103 58-59 -1.6357086294620444 1103 60-61 -1.6771298544651714 1103 62-63 -1.1158482778314252 1103 64-65 -0.9597896623281201 1103 66-67 0.02416838548108302 1103 68-69 0.0996617407858551 1103 70-71 0.004728102919621335 1103 72-73 0.2550029754415881 1103 74-75 -0.38376016372870936 1103 76-77 -0.3191459608491449 1103 78-79 0.4060001625670111 1103 80-81 0.36780305673774194 1103 82-83 0.4470039763241189 1103 84-85 0.7441511591692844 1103 86-87 -0.16188543931069788 1103 88-89 -0.544113271632181 1103 90-91 -0.02438638368852608 1103 92-93 -0.5265478389465166 1103 94-95 -0.17282586450339466 1103 96-97 0.39725919708384794 1103 98-99 0.2788662419268384 1103 100-101 0.6321504541609464 1104 1 0.31388706854748705 1104 2 0.26738334714653433 1104 3 0.23010947715331298 1104 4 0.2989961890305821 1104 5 0.16190994533848624 1104 6 0.11610643492397799 1104 7 0.3390099233976187 1104 8 0.3260689665898937 1104 9 0.5151147008060946 1104 10-11 0.5288416891094272 1104 12-13 0.4623216982680063 1104 14-15 0.5422344297009047 1104 16-17 0.7562964638210516 1104 18-19 0.8377660840015508 1104 20-21 0.4595428045465155 1104 22-23 0.2715646933159306 1104 24-25 0.29027992938153346 1104 26-27 0.44463242641104017 1104 28-29 0.6138914326295932 1104 30-31 0.6033960336002906 1104 32-33 1.1615800663217897 1104 34-35 1.2811580183752511 1104 36-37 0.7173344768231757 1104 38-39 -0.816709591630449 1104 40-41 -1.160245318460717 1104 42-43 -1.3597480722576805 1104 44-45 -1.1566144759576744 1104 46-47 -0.15664737765614234 1104 48-49 0.4973798773296636 1104 50-51 1.0790825447360461 1104 52-53 0.7765296225837588 1104 54-55 1.220844602615223 1104 56-57 0.8390035408300491 1104 58-59 0.34112063883063115 1104 60-61 0.5439067309006802 1104 62-63 0.6740907465588251 1104 64-65 1.5182591181596834 1104 66-67 0.9662415562127862 1104 68-69 0.5203934481029293 1104 70-71 0.5138744443830348 1104 72-73 0.13701517056354007 1104 74-75 0.18361788505177756 1104 76-77 0.1418296489069526 1104 78-79 0.2852684552499376 1104 80-81 0.7424981786889617 1104 82-83 0.8381625129094843 1104 84-85 0.8880535981936717 1104 86-87 0.457931633860035 1104 88-89 1.1757038015385533 1104 90-91 1.0057965431407396 1104 92-93 0.40942777080958237 1104 94-95 0.6582716964722195 1104 96-97 0.6695152946448246 1104 98-99 0.9331345346097635 1104 100-101 1.5672114297707012 1105 1 0.33162542774926607 1105 2 0.21195097464099177 1105 3 0.018912137907193483 1105 4 0.19256603381993642 1105 5 0.24561282782185856 1105 6 0.3067937963430438 1105 7 0.42548442450626567 1105 8 0.18028182690031258 1105 9 -0.2149296450919138 1105 10-11 0.02523858489657016 1105 12-13 0.11864098873363815 1105 14-15 0.027267689124407468 1105 16-17 0.17037628643746672 1105 18-19 0.14957317934523928 1105 20-21 0.42378892428043713 1105 22-23 0.06757356249553226 1105 24-25 0.2886169582063616 1105 26-27 0.2896989452580456 1105 28-29 0.19676282952537605 1105 30-31 0.0715224194096038 1105 32-33 0.17211221709784041 1105 34-35 -0.23519453151388348 1105 36-37 -0.41792051209034753 1105 38-39 0.308290408369551 1105 40-41 0.8818832846435001 1105 42-43 0.7253406195383292 1105 44-45 0.43845204288933104 1105 46-47 -1.1444522557049197 1105 48-49 -1.092420566129313 1105 50-51 -1.3521813133570788 1105 52-53 -1.2412087366180131 1105 54-55 -1.2137451978282314 1105 56-57 -0.5005197407663999 1105 58-59 -0.7334359021893206 1105 60-61 -0.8987207635560779 1105 62-63 -1.6033482778314223 1105 64-65 -2.5302442077826655 1105 66-67 -2.9417407054280105 1105 68-69 -2.729883713759598 1105 70-71 -3.199817351625832 1105 72-73 -2.468860660922047 1105 74-75 -3.5189874364559834 1105 76-77 -2.896418688121873 1105 78-79 -3.5326362010693515 1105 80-81 -2.803787852353164 1105 82-83 -2.2314051145849696 1105 84-85 -2.8103942953761702 1105 86-87 -2.294839984765243 1105 88-89 -2.622522362541268 1105 90-91 -2.5823409291430757 1105 92-93 -1.42427511167379 1105 94-95 -1.4046440463215717 1105 96-97 -1.8493317120070571 1105 98-99 -2.3620428489822523 1105 100-101 -1.4167131822026917 1106 1 0.21746052288668238 1106 2 0.14429769768538847 1106 3 -0.07675382403783715 1106 4 -0.3317468625859803 1106 5 0.001955322536446147 1106 6 0.25393967372148296 1106 7 0.04916307144072363 1106 8 0.2421211503675451 1106 9 0.22640227879814745 1106 10-11 0.3674690288711986 1106 12-13 -0.11708839815853622 1106 14-15 0.3459780485324444 1106 16-17 -0.007742106797209658 1106 18-19 0.6086112343135213 1106 20-21 0.725850235062687 1106 22-23 0.46186531725240343 1106 24-25 0.2891454994325713 1106 26-27 0.4242126873299341 1106 28-29 0.151044013457728 1106 30-31 0.3413427153926918 1106 32-33 0.46333843274265973 1106 34-35 -0.21061736449485835 1106 36-37 -0.3671805543736397 1106 38-39 -0.8864770334909124 1106 40-41 -1.8834444109167592 1106 42-43 -1.7474395073115687 1106 44-45 -1.5956388662015755 1106 46-47 -1.5398010929142174 1106 48-49 -2.4592281771229665 1106 50-51 -1.7422447383042226 1106 52-53 -2.580532203848456 1106 54-55 -2.3004259589275975 1106 56-57 -2.632919317933421 1106 58-59 -2.4926853736480936 1106 60-61 -2.1960252033023764 1106 62-63 -2.519045952250025 1106 64-65 -2.348161755351377 1106 66-67 -3.071761847077056 1106 68-69 -2.9061522127025157 1106 70-71 -2.42259747847573 1106 72-73 -2.9778458617677153 1106 74-75 -3.167771791635687 1106 76-77 -3.0819366585235635 1106 78-79 -3.4509765816190345 1106 80-81 -2.8278364781459793 1106 82-83 -2.643403000420065 1106 84-85 -3.1116627943190878 1106 86-87 -2.981943578845577 1106 88-89 -2.6130086204693868 1106 90-91 -2.4845608022931778 1106 92-93 -2.058524583132563 1106 94-95 -2.4234072598522296 1106 96-97 -1.9169849889626605 1106 98-99 -2.070552362724328 1106 100-101 -2.2745355923506843 1107 1 -0.11222483963041441 1107 2 -0.09464439077790843 1107 3 -0.357647576887814 1107 4 -0.3992343226863042 1107 5 0.0366110452906625 1107 6 -0.14596912700811515 1107 7 0.03867515534405186 1107 8 -0.4199855527788259 1107 9 0.1019152746941856 1107 10-11 -0.22008227071840736 1107 12-13 -0.20577968862821194 1107 14-15 -0.7557091379700651 1107 16-17 -0.0822975103539747 1107 18-19 0.07760348237554382 1107 20-21 -0.17269057661082599 1107 22-23 -0.32814836263815295 1107 24-25 -0.4203848243248345 1107 26-27 0.07423548714753281 1107 28-29 -0.7785045501537624 1107 30-31 -1.7170248176670455 1107 32-33 -2.0172817222960973 1107 34-35 -2.6939734976458 1107 36-37 -2.4134641841046047 1107 38-39 -2.640239003395159 1107 40-41 -2.0521630611675548 1107 42-43 -2.023545298465237 1107 44-45 -2.0368153367898074 1107 46-47 -1.899354216489229 1107 48-49 -1.5843991757549816 1107 50-51 -1.912787373963134 1107 52-53 -2.1502996457089196 1107 54-55 -1.4962763921241375 1107 56-57 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-1.6808044045131538 2316 50-51 -1.4891762628520269 2316 52-53 -0.9211329790422553 2316 54-55 -1.403139137222169 2316 56-57 -0.4809490336957012 2316 58-59 -1.0023752961287187 2316 60-61 -0.21185207668739992 2316 62-63 -1.2977927222758652 2316 64-65 -1.9778452178836758 2316 66-67 -1.1216649478522456 2316 68-69 -0.9628382592141449 2316 70-71 -0.09943856374704296 2316 72-73 0.264725197663811 2316 74-75 0.038462058493511364 2316 76-77 -0.48164596084914635 2316 78-79 -0.1551109485440989 2316 80-81 -0.06136360992892165 2316 82-83 1.033115087435231 2316 84-85 0.1580400480581723 2316 86-87 0.5700590051337464 2316 88-89 0.30866450614560037 2316 90-91 -0.8424419392440861 2316 92-93 -0.08210339450207371 2316 94-95 0.7563408021632725 2316 96-97 0.9486480859727386 2316 98-99 1.0621995752601734 2316 100-101 1.2404837874942807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 13.0 15 34.0 16 125.0 17 302.0 18 584.0 19 722.0 20 844.0 21 990.0 22 1031.0 23 732.0 24 675.0 25 635.0 26 650.0 27 744.0 28 917.0 29 1193.0 30 860.0 31 698.0 32 832.0 33 1006.0 34 1430.0 35 4505.0 36 3928.0 37 7745.0 38 15424.0 39 3902.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11393860118517 22.504310601105892 15.064312186614345 19.317438611094595 2 15.585598068130086 24.807505789671623 40.73553571782031 18.871360424377983 3 17.82427109518814 29.3422537162764 37.71303022505493 15.120444963480534 4 9.45943271114982 25.26474139466756 40.01108449951506 25.26474139466756 5 8.242117139407375 40.62271134775638 38.72647018071693 12.408701332119316 6 19.550286019674985 42.53280813918964 19.172225411215138 18.744680429920233 7 17.945012964905683 39.9675382514202 24.69468141960769 17.392767364066426 8 21.335682191563905 35.30215158053087 24.28297143762 19.07919479028523 9 22.509451515211495 17.602581104887076 25.28453514380159 34.60343223609984 10-11 20.29354129965757 28.565349062765975 31.31668019239524 19.824429445181213 12-13 23.19431523524871 29.23932622077948 30.5239405395776 17.042418004394214 14-15 19.776924447259557 29.88262306763524 30.788187090516818 19.55226539458839 16-17 15.909225866471369 34.55493755072148 31.51065893390867 18.025177648898477 18-19 16.026998673818806 31.656142990043744 35.26355376971952 17.053304566417925 20-21 16.570337087547752 29.745056511153777 35.36945032758655 18.315156073711922 22-23 16.387244908058037 31.782822984501497 34.737040042754494 17.092892064685973 24-25 17.51054017141387 33.11593198867798 34.61431879812355 14.759209041784604 26-27 17.052314878961226 31.722452049642726 36.65505433384138 14.57017873755468 28-29 15.454959323845529 30.838661150808576 37.612082104471405 16.094297420874486 30-31 17.571900793729338 30.65754834623226 36.02561311137943 15.744937748658975 32-33 16.625759585123017 30.06472555966826 37.47253617307654 15.836978682132182 34-35 15.205558084756834 31.843193919360267 37.87929771778072 15.071950278102175 36-37 17.374952989845806 30.762455216642586 35.64656281546288 16.216028978048733 38-39 15.205558084756834 30.627857722531225 36.71245620633004 17.454127986381902 40-41 14.7562399794145 30.84163021317868 37.58041210585697 16.82171770154985 42-43 16.30708022406524 30.567486787672454 36.698600581936226 16.426832406326085 44-45 15.224362146434157 30.646661784208547 36.91930088478059 17.20967518457671 46-47 17.711446725124205 29.945963064864117 34.55295817580808 17.7896320342036 48-49 16.572316462461153 29.229429346212466 35.16854377387621 19.02971041745017 50-51 14.110963757645337 30.04394212307753 36.53332277666713 19.311771342610005 52-53 15.644979315532154 30.988103956770452 36.90643494784347 16.46048177985392 54-55 14.846301537974307 29.597593080105298 35.50404782169791 20.05205756022248 56-57 16.954335820747808 30.334910235347678 33.66520852714713 19.04554541675739 58-59 15.82510243265177 30.70109459432711 35.894974367094875 17.578828605926248 60-61 16.952356445834404 32.62108826032739 33.17927198590685 17.247283307931355 62-63 14.57809623720829 34.02149601155955 32.88929356109341 18.511114190138755 64-65 14.796817165139247 34.492587240949305 32.06686328457473 18.64373230933671 66-67 15.080857465212485 33.27725104412026 31.873874230518002 19.768017260149247 68-69 15.07491934047228 32.2984501494428 31.97284297618812 20.653787533896796 70-71 15.872607430573424 32.725995130737715 31.854080481383978 19.547316957304883 72-73 16.728687080619938 30.44476554304151 32.68838700738307 20.138160368955486 74-75 15.412402763207378 31.725421112012825 31.72344173709942 21.138734387680373 76-77 16.151699293363155 31.336473941529263 32.05696641000772 20.45486035509986 78-79 15.98543180063736 31.438411749569482 31.666039864610756 20.9101165851824 80-81 16.631697709863225 30.20229211614972 31.890698917281924 21.27531125670513 82-83 16.05372023514974 31.176144573543674 32.077749846598444 20.692385344708143 84-85 16.066586172086854 30.62488866016112 31.894657667108728 21.413867500643295 86-87 16.34072959759308 30.97919676966014 31.48492706003444 21.195146572712336 88-89 16.588151461768376 30.690208032303403 30.753548029532276 21.968092476395952 90-91 16.518873339799292 29.78266463450842 32.43601670592427 21.262445319768016 92-93 16.83062488866016 29.868767443241424 32.61712951050059 20.683478157597833 94-95 16.6099245858158 28.88699748619386 32.615150135587186 21.887927792403158 96-97 16.431780843609587 28.62473031016805 33.266364482096556 21.67712436412581 98-99 16.336770847766275 29.010708418281506 31.99362641277884 22.658894321173374 100-101 15.723164624611547 28.91470873498149 32.8041804398171 22.557946200589853 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1916.0 1 1098.0 2 318.5 3 408.0 4 294.5 5 154.5 6 232.0 7 436.0 8 619.0 9 587.5 10 349.5 11 160.0 12 145.0 13 133.5 14 127.0 15 133.5 16 130.5 17 132.5 18 140.5 19 150.5 20 159.0 21 171.5 22 203.0 23 220.5 24 230.0 25 311.5 26 517.5 27 681.0 28 718.5 29 858.0 30 1081.5 31 1240.0 32 1366.0 33 1512.5 34 1804.0 35 2125.5 36 2301.0 37 2326.0 38 2032.0 39 1689.0 40 1558.5 41 1527.5 42 1497.5 43 1435.0 44 1342.0 45 1245.0 46 1196.5 47 1388.0 48 1988.0 49 2070.0 50 1634.0 51 1358.0 52 890.0 53 476.5 54 353.5 55 329.5 56 281.0 57 220.5 58 204.5 59 162.0 60 133.5 61 119.0 62 122.5 63 106.0 64 68.0 65 71.5 66 79.0 67 66.5 68 62.5 69 47.0 70 25.0 71 29.5 72 28.5 73 18.5 74 19.5 75 20.0 76 18.5 77 14.0 78 11.0 79 12.5 80 10.0 81 8.5 82 6.0 83 3.0 84 3.0 85 2.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12667999445775024 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 50521.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.027750836285904 #Duplication Level Percentage of deduplicated Percentage of total 1 72.99604413907974 27.758753785554525 2 11.664584634603372 8.871558361869322 3 4.861544867790964 5.546208507353378 4 2.7118467624401417 4.125017319530492 5 1.6864459712679576 3.206587359711803 6 1.2179887570268582 2.779042378416896 7 0.9941703102227775 2.646424259218939 8 0.4944826150322715 1.504324934185784 9 0.38517593170934833 1.3182636923259634 >10 2.6858213616489697 18.695196057085173 >50 0.18738288569643974 4.997921656340928 >100 0.08328128253175099 6.721957205914372 >500 0.01561524047470331 4.198254191326379 >1k 0.01561524047470331 7.6304902911660495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1336 2.6444448843055364 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1307 2.5870430118168684 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1212 2.399002395043645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 768 1.520159933493003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 759 1.5023455592723818 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 1.1757486985609944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.8412343381959977 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 411 0.8135230894083647 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 342 0.6769462203836029 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 334 0.6611112210763841 TruSeq Adapter, Index 1 (95% over 21bp) GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 302 0.597771223847509 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 262 0.5185962273114151 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 197 0.38993685794026245 RNA PCR Primer, Index 1 (95% over 23bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 169 0.3345143603649967 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.30680311157736384 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.2632568634825122 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.24544248926189108 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 120 0.23752498960828172 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 109 0.2157518655608559 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105 0.2078343659072465 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 104 0.20585499099384413 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 104 0.20585499099384413 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 98 0.19397874151343006 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 98 0.19397874151343006 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 91 0.18012311711961362 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 89 0.17616436729280893 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 87 0.17220561746600424 TruSeq Adapter, Index 1 (96% over 26bp) ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 83 0.16428811781239483 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 83 0.16428811781239483 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 81 0.16032936798559017 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 80 0.1583499930721878 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 79 0.15637061815878545 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 79 0.15637061815878545 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 79 0.15637061815878545 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 79 0.15637061815878545 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 77 0.15241186833198075 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 76 0.15043249341857842 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75 0.14845311850517606 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 73 0.14449436867837137 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 71 0.14053561885156668 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 71 0.14053561885156668 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 67 0.1326181191979573 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 67 0.1326181191979573 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 63 0.1247006195443479 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 62 0.12272124463094554 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 62 0.12272124463094554 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 58 0.11480374497733616 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 56 0.11084499515053148 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 56 0.11084499515053148 TruSeq Adapter, Index 1 (96% over 28bp) CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 55 0.1088656202371291 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 55 0.1088656202371291 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 55 0.1088656202371291 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 54 0.10688624532372677 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.10688624532372677 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 54 0.10688624532372677 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 53 0.10490687041032443 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 53 0.10490687041032443 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 52 0.10292749549692207 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0019793749134023476 0.0 2 0.0 0.0 0.0 0.0019793749134023476 0.0 3 0.0 0.0 0.0 0.0019793749134023476 0.0 4 0.0 0.0 0.0 0.0019793749134023476 0.0 5 0.0 0.0 0.0 0.0019793749134023476 0.0 6 0.0 0.0 0.0 0.0019793749134023476 0.0 7 0.0 0.0 0.0 0.0019793749134023476 0.0 8 0.0 0.0 0.0 0.0019793749134023476 0.0 9 0.0 0.0 0.0 0.0019793749134023476 0.0 10-11 0.0 0.0 0.0 0.0019793749134023476 0.0 12-13 0.0 0.0 0.0 0.003958749826804695 0.0 14-15 0.0 0.0 0.0 0.003958749826804695 0.0 16-17 0.0 0.0 0.0 0.003958749826804695 0.0 18-19 0.0 0.0 0.0 0.003958749826804695 0.0 20-21 0.0 0.0 0.0 0.003958749826804695 0.0 22-23 0.0 0.0 0.0 0.0069278121969082165 0.0 24-25 0.0 0.0 0.0 0.02870093624433404 0.0 26-27 0.0 0.0 0.0 0.07224718433918569 0.0 28-29 0.0 0.0 0.0 0.17913342966291246 0.0 30-31 0.0 0.0 0.0 0.3661843589794343 0.0 32-33 0.0 0.0 0.0 0.570059975059876 0.0 34-35 0.0 0.0 0.0 0.8392549632825954 0.0 36-37 0.0 0.0 0.0 1.1886146354981095 0.0 38-39 0.0 0.0 0.0 1.5924071178321886 0.0 40-41 0.0 0.0 0.0 2.0981374082064885 0.0 42-43 0.0 0.0 0.0 2.483125828863245 0.0 44-45 0.0 0.0 0.0 2.77409394113339 0.0 46-47 0.0 0.0 0.0 3.0175570554818787 0.0 48-49 0.0 0.0 0.0 3.2432057956097466 0.0 50-51 0.0 0.0 0.0 3.506462659092259 0.0 52-53 0.0 0.0 0.0 3.726173274479919 0.0 54-55 0.0 0.0 0.0 4.011203262009857 0.0 56-57 0.0 0.0 0.0 4.396191682666615 0.0 58-59 0.0 0.0 0.0 4.725757605748105 0.0 60-61 0.0 0.0 0.0 5.029591654955365 0.0 62-63 0.0 0.0 0.0 5.283941331327567 0.0 64-65 0.0 0.0 0.0 5.554126007006987 0.0 66-67 0.0 0.0 0.0 5.891609429742088 0.0 68-69 0.0 0.0 0.0 6.381504720809168 0.0 70-71 0.0 0.0 0.0 6.862492824765939 0.0 72-73 0.0 0.0 0.0 7.351398428376319 0.0 74-75 0.0 0.0 0.0 7.788840284238237 0.0 76-77 0.0 0.0 0.0 8.225292452643455 0.0 78-79 0.0 0.0 0.0 8.697373369489915 0.0 80-81 0.0 0.0 0.0 9.226856158825044 0.0 82-83 0.0 0.0 0.0 9.728627699372538 0.0 84-85 0.0 0.0 0.0 10.170028305061262 0.0 86-87 0.0 0.0 0.0 10.610439223293284 0.0 88-89 0.0 0.0 0.0 11.18940638546347 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 85 1.2369128E-10 50.293594 9 ACCCATG 40 5.3393695E-4 47.499508 3 GTACCCA 45 9.4917923E-4 42.263626 1 CAGCCTC 50 0.0016013082 37.999603 5 AGCCTCT 55 0.0025565678 34.545094 6 TCAGCCT 55 0.0025565678 34.545094 4 TACCCAT 55 0.0025565678 34.545094 2 GGTACTC 30 0.0039342595 31.666338 50-51 CAGAAAA 115 0.0 28.912743 10-11 TGAAAAA 285 0.0 27.499714 58-59 CTTGAAA 300 0.0 26.916386 56-57 GGTATCA 850 0.0 26.849834 1 TTGAAAA 280 0.0 26.29437 58-59 TGCTTGA 320 0.0 25.234112 54-55 TCTGCTT 330 0.0 24.469442 52-53 ATTAACC 100 0.0016320264 23.749752 8 ATAGATT 100 0.0016320264 23.749752 4 GAGAAAA 90 1.914832E-7 23.74975 12-13 CTGCTTG 315 0.0 23.372772 54-55 GCAGAAA 105 8.4500607E-7 22.61881 9 >>END_MODULE