##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031859_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57884 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30500656485385 33.0 31.0 34.0 30.0 34.0 2 32.37176076290512 34.0 31.0 34.0 30.0 34.0 3 32.29814802017829 34.0 31.0 34.0 30.0 34.0 4 35.892146361688894 37.0 35.0 37.0 35.0 37.0 5 35.937046506806716 37.0 35.0 37.0 35.0 37.0 6 36.13283463478682 37.0 36.0 37.0 35.0 37.0 7 36.0594637550964 37.0 36.0 37.0 35.0 37.0 8 36.135356920738026 37.0 37.0 37.0 35.0 37.0 9 37.788110704166954 39.0 38.0 39.0 35.0 39.0 10-11 37.820537626978094 39.0 38.0 39.0 35.0 39.0 12-13 37.82645463340474 39.0 38.0 39.0 35.0 39.0 14-15 38.993253748877066 41.0 38.0 41.0 35.5 41.0 16-17 38.951152304609224 40.0 38.5 41.0 35.5 41.0 18-19 39.154541842305306 41.0 39.0 41.0 35.5 41.0 20-21 39.22200435353466 41.0 39.0 41.0 35.0 41.0 22-23 38.99263181535485 41.0 38.5 41.0 35.0 41.0 24-25 38.66364625803331 40.5 38.0 41.0 35.0 41.0 26-27 38.19594361136065 40.0 36.5 41.0 34.5 41.0 28-29 37.846978439637894 40.0 35.0 41.0 34.0 41.0 30-31 37.223179116854396 39.0 35.0 41.0 33.0 41.0 32-33 36.036011678529476 37.5 35.0 40.5 30.5 41.0 34-35 35.49457535761178 37.0 35.0 40.0 30.0 41.0 36-37 34.86578329071937 37.0 35.0 40.0 24.5 41.0 38-39 33.83427199226038 35.5 34.5 40.0 17.0 41.0 40-41 33.372667749291686 36.0 33.5 40.0 12.0 41.0 42-43 32.51165261557598 35.5 33.0 40.0 8.5 41.0 44-45 31.48186891023426 35.0 29.5 40.0 7.0 41.0 46-47 30.339817566166815 35.0 22.0 40.0 7.0 41.0 48-49 29.254716329210147 35.0 16.5 40.0 7.0 41.0 50-51 28.351850252228594 35.0 10.0 40.0 7.0 41.0 52-53 28.30960196254578 35.0 10.0 40.0 7.0 41.0 54-55 27.918198465897312 35.0 10.0 39.5 7.0 41.0 56-57 27.40624352152581 35.0 10.0 39.0 7.0 41.0 58-59 26.996907608320086 35.0 10.0 39.0 7.0 41.0 60-61 26.192678460369017 34.5 8.0 38.5 7.0 41.0 62-63 25.483181881003386 33.0 8.0 37.0 7.0 41.0 64-65 24.795133370188655 32.5 7.0 37.0 7.0 39.5 66-67 23.7464584341096 29.5 7.0 36.0 7.0 39.0 68-69 22.53742830488563 24.5 7.0 35.0 7.0 39.0 70-71 21.464463409577775 20.5 7.0 35.0 7.0 37.0 72-73 20.75491500241863 18.5 7.0 35.0 7.0 37.0 74-75 20.595069449243315 19.0 7.0 35.0 7.0 36.0 76-77 19.766386220717298 16.5 7.0 33.5 5.0 35.0 78-79 20.36164570520351 19.0 7.0 35.0 5.0 35.0 80-81 20.386592149816877 19.0 7.0 35.0 5.0 35.0 82-83 20.29277520558358 19.0 7.0 35.0 5.0 35.0 84-85 20.184740169995166 18.5 7.0 35.0 5.0 35.0 86-87 20.18056803261696 18.0 7.0 35.0 7.0 35.0 88-89 20.072325685854466 18.0 7.0 35.0 7.0 35.0 90-91 19.92207691244558 17.5 7.0 35.0 7.0 35.0 92-93 19.885106419736026 17.0 7.0 35.0 7.0 35.0 94-95 19.803063022596916 17.0 7.0 35.0 7.0 35.0 96-97 19.796005804712873 17.0 7.0 35.0 7.0 35.0 98-99 19.782487388570246 17.0 7.0 35.0 7.0 35.0 100-101 18.954279248151476 13.5 7.0 33.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3269833601342995 1101 2 0.11285345478307818 1101 3 -0.07663692566025304 1101 4 0.30970512494715763 1101 5 0.053656539951326465 1101 6 0.06800218763993371 1101 7 0.23729531415524718 1101 8 0.052217476420565845 1101 9 0.6656768408399856 1101 10-11 0.46595327966831945 1101 12-13 0.14688074200888224 1101 14-15 0.1966400971052309 1101 16-17 -0.4024758166071578 1101 18-19 0.006829798883217109 1101 20-21 0.26380762945993297 1101 22-23 -0.3789007890690286 1101 24-25 -0.2580878650231071 1101 26-27 0.06324537171303035 1101 28-29 0.04714225245861314 1101 30-31 -0.41547767505031175 1101 32-33 -0.8066543235905357 1101 34-35 -0.2115842008417701 1101 36-37 -0.616900651522009 1101 38-39 -0.5354223430470881 1101 40-41 -0.6219653607209352 1101 42-43 -1.0793222282027202 1101 44-45 -1.0668513039821477 1101 46-47 -0.6749278912443728 1101 48-49 -0.40641635463570225 1101 50-51 -0.22950499278408287 1101 52-53 0.30439274495440216 1101 54-55 0.9469453586931316 1101 56-57 0.14681903778390293 1101 58-59 0.03338279708757241 1101 60-61 1.4406180736206409 1101 62-63 1.125244476990396 1101 64-65 -0.4570652763530738 1101 66-67 0.10114771601234551 1101 68-69 -0.3445228480022102 1101 70-71 0.6800319131893069 1101 72-73 1.3857760548692255 1101 74-75 1.1920248120846786 1101 76-77 0.7077255245415763 1101 78-79 1.2792207948921295 1101 80-81 0.8126526483502765 1101 82-83 1.1727153955763931 1101 84-85 1.2161914667119404 1101 86-87 1.3435871384177425 1101 88-89 2.474686465404414 1101 90-91 1.798424750014334 1101 92-93 1.8513612393014363 1101 94-95 1.7254542242412256 1101 96-97 1.1014264394193098 1101 98-99 1.2774614534044169 1101 100-101 1.0766973264180173 1102 1 0.27055412794188527 1102 2 0.2506887924334009 1102 3 0.0667488282712938 1102 4 -0.035345753868753604 1102 5 -0.13783282175079847 1102 6 -4.5294649513039076E-4 1102 7 0.33674027252712335 1102 8 0.4735865791032623 1102 9 0.5070274421350831 1102 10-11 0.43820119826221315 1102 12-13 -0.05547818491063339 1102 14-15 0.702190513386455 1102 16-17 -0.16958960014091673 1102 18-19 0.2475791050811793 1102 20-21 0.635222985611648 1102 22-23 0.40486424330839554 1102 24-25 0.6790074171230529 1102 26-27 0.928879044238478 1102 28-29 0.6750330942717468 1102 30-31 1.0234677458562587 1102 32-33 0.7731329104520199 1102 34-35 -0.11306431185009558 1102 36-37 -0.33637336197529777 1102 38-39 0.4557894978409749 1102 40-41 1.0292834829423398 1102 42-43 1.9516675960341061 1102 44-45 2.1556278819568035 1102 46-47 1.3287723862764445 1102 48-49 0.23928207274635227 1102 50-51 -1.155036907677701 1102 52-53 -0.8450059599484625 1102 54-55 -0.4210009872828131 1102 56-57 -1.3848645884880675 1102 58-59 -2.4969132251140884 1102 60-61 -2.5263106960370827 1102 62-63 -3.287799001270475 1102 64-65 -3.0005435372226366 1102 66-67 -1.3993148186777553 1102 68-69 -1.4173720616932322 1102 70-71 -1.530421370806991 1102 72-73 -1.6634838896266082 1102 74-75 -1.6141731527626852 1102 76-77 -1.4754382127387196 1102 78-79 -1.545016022869202 1102 80-81 -0.8839245949429717 1102 82-83 -0.6369515794467304 1102 84-85 -0.46350326039259215 1102 86-87 0.3109784427655704 1102 88-89 -0.4210119619776407 1102 90-91 -1.4733143804204474 1102 92-93 -1.6208866792924574 1102 94-95 -1.3506327322805163 1102 96-97 -1.856020369091329 1102 98-99 -1.216063060934161 1102 100-101 -1.1365311657188926 1103 1 0.3636983359797128 1103 2 0.39111432434829396 1103 3 -0.15972871309986658 1103 4 -0.08546395717844746 1103 5 -0.12412123782645068 1103 6 -0.5493891167078928 1103 7 -0.2221249756998276 1103 8 -0.254545808603595 1103 9 0.03137732393177117 1103 10-11 0.28382767580358603 1103 12-13 0.0026778434581586907 1103 14-15 0.2019541067670687 1103 16-17 -0.0012680871385555292 1103 18-19 0.4776027457431127 1103 20-21 0.3314404797014845 1103 22-23 0.269166843781214 1103 24-25 -0.17016515970910007 1103 26-27 -0.06308313070242377 1103 28-29 0.13578959545377955 1103 30-31 -0.562820670219395 1103 32-33 -0.23443210136831283 1103 34-35 -0.08114941823307475 1103 36-37 0.6012152905069712 1103 38-39 0.11820084535870024 1103 40-41 -0.34660304188034985 1103 42-43 -0.6228004890722829 1103 44-45 -0.6881073426294861 1103 46-47 0.37676293001166883 1103 48-49 0.008076398987487465 1103 50-51 -0.018393881672974288 1103 52-53 -0.16179082992482208 1103 54-55 -0.43542179106531975 1103 56-57 -1.6756447303320385 1103 58-59 -2.0571969130573535 1103 60-61 -1.5272563225146243 1103 62-63 -2.3877990012704764 1103 64-65 -1.3338768705559723 1103 66-67 -2.0539247477557687 1103 68-69 -1.4684358914804676 1103 70-71 -1.2001613235256663 1103 72-73 -0.01833022532401074 1103 74-75 -1.3881684246302974 1103 76-77 -1.3787479054101155 1103 78-79 -1.4043540843349227 1103 80-81 -1.9598111197656642 1103 82-83 -1.8705213193994474 1103 84-85 -2.0294607072011033 1103 86-87 -1.600132668345541 1103 88-89 -1.3564729548854437 1103 90-91 -0.873314380420446 1103 92-93 -2.2887353790560496 1103 94-95 -1.0061882878360713 1103 96-97 -0.25412911613624445 1103 98-99 -0.8848573871752947 1103 100-101 -0.9117084706834326 1104 1 0.0289761137574871 1104 2 0.4036143243482968 1104 3 0.5374935091223563 1104 4 0.4048138205993297 1104 5 0.15782320661799076 1104 6 -0.21188911670789423 1104 7 -0.026291642366494727 1104 8 0.14823196917418358 1104 9 0.10498843504288402 1104 10-11 0.19285545358136602 1104 12-13 0.20892784345815585 1104 14-15 0.22834299565595728 1104 16-17 -0.23807364269411124 1104 18-19 -0.3029528098124388 1104 20-21 0.011996035257041626 1104 22-23 -0.1565276006632388 1104 24-25 -0.766692937486873 1104 26-27 -0.6790553529246495 1104 28-29 -0.5044881823239962 1104 30-31 -0.6600428924416164 1104 32-33 -0.6191543235905357 1104 34-35 -2.247816084899746 1104 36-37 -2.6821180428263602 1104 38-39 -1.8755491546412912 1104 40-41 -2.127853041880357 1104 42-43 -1.966550489072283 1104 44-45 -0.7874128981850461 1104 46-47 -1.0322648477661112 1104 48-49 -0.558590267679179 1104 50-51 -0.6565883261174186 1104 52-53 -0.8110963854803792 1104 54-55 -0.8673662355097633 1104 56-57 -0.061061396998706385 1104 58-59 0.2969697536093143 1104 60-61 -0.7862841002924021 1104 62-63 -1.0273823346038071 1104 64-65 -0.7609602038893009 1104 66-67 -1.3893414144224359 1104 68-69 -1.1746858914804683 1104 70-71 -1.1279391013034434 1104 72-73 -0.2940246697684543 1104 74-75 -0.5221962024080717 1104 76-77 -0.6926367942990055 1104 78-79 -1.0015763065571441 1104 80-81 -0.6764777864323328 1104 82-83 -0.21496576384389243 1104 84-85 -0.07321070720110257 1104 86-87 -1.4911048905677617 1104 88-89 -0.8835562882187773 1104 90-91 -0.5358143804204474 1104 92-93 -0.9692909346116068 1104 94-95 -0.2985493989471806 1104 96-97 -0.6902402272473545 1104 98-99 -0.28277405384196186 1104 100-101 -1.257541804016764 1105 1 0.13480944709082365 1105 2 0.15111432434829908 1105 3 -0.39750649087764245 1105 4 -0.18768617940067145 1105 5 -0.5263434600486718 1105 6 0.15061088329210293 1105 7 0.07120835763350186 1105 8 -0.26343469749247816 1105 9 0.06915510170954775 1105 10-11 -0.4483945464186334 1105 12-13 -0.28398882320851015 1105 14-15 0.057509662322623 1105 16-17 -0.3468236426941118 1105 18-19 -0.14795280981243764 1105 20-21 -0.14967063140962722 1105 22-23 0.14805573267009464 1105 24-25 0.06539039584645678 1105 26-27 0.058027980408688506 1105 28-29 0.4580118176760024 1105 30-31 -0.2650428924416204 1105 32-33 1.0733456764094598 1105 34-35 0.6388505817669241 1105 36-37 -0.08211804282635882 1105 38-39 0.7315341786920371 1105 40-41 0.4367302914529816 1105 42-43 1.3071995109277168 1105 44-45 2.0996704351482833 1105 46-47 1.3289851522338871 1105 48-49 1.061409732320822 1105 50-51 0.5904950072159174 1105 52-53 0.44265361451962093 1105 54-55 0.33346709782356854 1105 56-57 0.9176886030012952 1105 58-59 0.15946975360931503 1105 60-61 -0.9950341002924041 1105 62-63 -1.3377990012704721 1105 64-65 -2.2605435372226346 1105 66-67 -1.080591414422436 1105 68-69 -1.4884358914804672 1105 70-71 -2.3712724346367793 1105 72-73 -1.629441336435125 1105 74-75 -0.82927953574141 1105 76-77 -1.4509701276323383 1105 78-79 -1.979909639890483 1105 80-81 -2.756477786432331 1105 82-83 -1.3316324305105631 1105 84-85 -2.3994607072011043 1105 86-87 -2.6290215572344273 1105 88-89 -1.9031396215521106 1105 90-91 -2.113314380420448 1105 92-93 -2.334290934611605 1105 94-95 -0.7506327322805149 1105 96-97 -2.2085735605806924 1105 98-99 -2.838190720508628 1105 100-101 -1.5650418040167615 1106 1 0.3785131507945252 1106 2 0.16518839842237298 1106 3 0.14397499060383723 1106 4 0.3515730798585892 1106 5 0.1462491325439217 1106 6 0.3135738462550677 1106 7 0.3741713205964672 1106 8 0.30100974695196214 1106 9 0.46841436096880784 1106 10-11 -0.04209825012233637 1106 12-13 0.3582333990137201 1106 14-15 0.19825040306336206 1106 16-17 -0.1049717908422565 1106 18-19 -0.020545402405033997 1106 20-21 0.22958862784962975 1106 22-23 -0.02342574881138404 1106 24-25 0.5168718773279366 1106 26-27 0.4517316841123957 1106 28-29 0.900604410268599 1106 30-31 0.9742163668176431 1106 32-33 1.545197528261312 1106 34-35 0.6966283595447038 1106 36-37 0.5864004756921588 1106 38-39 1.6256082527661135 1106 40-41 1.3756191803418645 1106 42-43 1.6734958072240076 1106 44-45 2.5841148795927324 1106 46-47 2.8137999670486984 1106 48-49 2.8765949175060044 1106 50-51 2.251976488697398 1106 52-53 2.754875836741842 1106 54-55 2.338652283008752 1106 56-57 1.9447256400383317 1106 58-59 2.035395679535238 1106 60-61 2.4023733071150026 1106 62-63 2.4714602579887845 1106 64-65 1.5550120183329206 1106 66-67 1.2460752522442284 1106 68-69 2.361193738149158 1106 70-71 2.6405794172150756 1106 72-73 3.237225330231542 1106 74-75 3.1803500938882188 1106 76-77 2.247178020515811 1106 78-79 2.410460730479894 1106 80-81 3.3420407320861862 1106 82-83 3.2757749768968445 1106 84-85 2.705724477984081 1106 86-87 2.764682146469273 1106 88-89 3.1268603784478906 1106 90-91 2.961870804764736 1106 92-93 1.766820176499504 1106 94-95 2.084552452904667 1106 96-97 2.1292042171970884 1106 98-99 1.4706981683802631 1106 100-101 1.7679211589461978 1107 1 0.46428313130135024 1107 2 0.310412569962331 1107 3 -0.4954012277197499 1107 4 0.00459452235371316 1107 5 0.29927057503904564 1107 6 -0.18447683600614084 1107 7 0.3322609892124504 1107 8 0.312705653384711 1107 9 -0.0013712140799242434 1107 10-11 0.06511422551118784 1107 12-13 0.004432229423066758 1107 14-15 0.18557983776122455 1107 16-17 -0.30770083567656314 1107 18-19 0.04134543580159544 1107 20-21 0.0010311229763360075 1107 22-23 0.16858204845956948 1107 24-25 0.28539039584645565 1107 26-27 0.43223850672447384 1107 28-29 0.09309953697425044 1107 30-31 0.05706237071627385 1107 32-33 1.0310649746550808 1107 34-35 1.1381488273809595 1107 36-37 1.4450749396297766 1107 38-39 2.9462710207972975 1107 40-41 2.7411162563652596 1107 42-43 1.7193047740856144 1107 44-45 1.2054599088324949 1107 46-47 1.2533711171461697 1107 48-49 1.7571992060050334 1107 50-51 1.8143546563387254 1107 52-53 1.5194957197827783 1107 54-55 1.5920635890516373 1107 56-57 1.7103201819486635 1107 58-59 1.1094697536093143 1107 60-61 -0.02257795994152545 1107 62-63 0.22974485837864833 1107 64-65 0.2275266382159593 1107 66-67 -0.2451528179312099 1107 68-69 -1.5929972949892424 1107 70-71 -2.097237346917481 1107 72-73 -1.8504939680140708 1107 74-75 -1.4185777813554417 1107 76-77 -0.7255315311411081 1107 78-79 -1.9511377100659182 1107 80-81 -1.6019163829235588 1107 82-83 -0.337772781387752 1107 84-85 -1.3610396545695238 1107 86-87 -2.0135829607432036 1107 88-89 -1.737174709271411 1107 90-91 -1.1399810470871117 1107 92-93 -1.037273390751956 1107 94-95 -0.41203624105244785 1107 96-97 -0.23190689391402586 1107 98-99 -0.2076644047191536 1107 100-101 -0.3660944355957092 1108 1 0.12308530915979077 1108 2 -0.1847477446172192 1108 3 -0.02716166329143732 1108 4 0.12679657922002008 1108 5 -0.0842744945314351 1108 6 -0.3390442891216878 1108 7 -0.48534336650442356 1108 8 -0.16067607680282237 1108 9 -0.42877593277321324 1108 10-11 -0.3039117877979507 1108 12-13 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-0.39685936031473545 2316 60-61 -0.916426505355691 2316 62-63 -0.8890648240552856 2316 64-65 -1.0068726511466863 2316 66-67 -0.05350280682749897 2316 68-69 -0.02160044844249498 2316 70-71 0.9849300970087889 2316 72-73 1.5768877774889312 2316 74-75 2.0222394516003668 2316 76-77 1.6749792394562704 2316 78-79 0.9578118790968624 2316 80-81 1.21276271989678 2316 82-83 1.3525447846793135 2316 84-85 1.3844633434318077 2316 86-87 1.551484771879494 2316 88-89 1.38951860629599 2316 90-91 1.014027391731453 2316 92-93 0.8519115970339612 2316 94-95 0.9848103056941682 2316 96-97 1.1773758065079143 2316 98-99 1.4742143427825134 2316 100-101 1.6182493352237408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 14.0 14 55.0 15 212.0 16 657.0 17 1633.0 18 2404.0 19 2826.0 20 3487.0 21 3924.0 22 3118.0 23 1839.0 24 1471.0 25 1401.0 26 1676.0 27 2085.0 28 2716.0 29 3091.0 30 1469.0 31 730.0 32 773.0 33 871.0 34 1197.0 35 2185.0 36 3846.0 37 7475.0 38 6503.0 39 225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.484848484848484 18.51044693510447 12.755984502559844 20.2487200774872 2 16.647087278004285 22.185750811968767 41.29984106143321 19.86732084859374 3 20.938428581300535 31.604588487319468 36.73554004560845 10.721442885771543 4 9.106143321125009 26.81570036624974 30.091216916591808 33.98693939603344 5 7.343998341510607 39.71736576601479 36.93248566097713 16.00615023149748 6 18.702923087554417 38.48559187340197 15.168267569622001 27.6432174694216 7 14.154170409785088 39.373574735678254 19.542533342547163 26.929721511989495 8 22.603828346347868 32.986662981134685 23.339783014304473 21.069725658212977 9 22.23066823301776 13.722272130467832 19.361136065233918 44.6859235712805 10-11 21.452387533688068 27.055835809550132 26.18512887844655 25.30664777831525 12-13 27.316702370257754 25.3930274341787 27.408264805473014 19.882005390090526 14-15 22.268675281597677 26.281874093013613 27.703683228526018 23.74576739686269 16-17 17.07898555732154 34.366146085273996 28.01033100684127 20.544537350563196 18-19 17.292343307304264 27.794381867182643 33.92215465413585 20.991120171377236 20-21 17.17832216156451 25.562331559671065 33.890194181466384 23.369152097298045 22-23 14.93590629534932 32.30253610669615 33.90747011263907 18.854087485315457 24-25 18.467106627047198 33.35118512887845 34.00767051344067 14.174037730633682 26-27 19.2255200055283 31.48106557943473 36.62756547577914 12.665848939257826 28-29 14.9479994471702 28.203821435975403 39.628394720475434 17.219784396378966 30-31 18.09999308962753 31.699606108769263 35.79572938981411 14.404671411789097 32-33 17.172275585654067 27.790062884389467 38.36984313454495 16.667818395411512 34-35 11.864245732845001 32.242070347591735 41.81898279317255 14.074701126390712 36-37 17.148953078570937 31.29362172621104 35.58755441918319 15.969870776034828 38-39 14.280284707345725 27.248462442125632 38.56246976712045 19.908783083408196 40-41 13.280872089005596 27.60434662428305 39.37098334600235 19.743797940709005 42-43 17.68379590038612 26.025551322915856 38.448780740625565 17.841872036072456 44-45 13.001002004008017 28.24182848455532 38.48818326307788 20.268986248358786 46-47 18.668532483350177 23.50583499615607 33.98291395648155 23.8427185640122 48-49 17.620738206915615 22.046006202112864 33.81101695647292 26.522238634498606 50-51 13.304309518256499 22.331061528760355 38.85304102205291 25.51158793093023 52-53 14.66924658362559 22.998116890969715 40.68465698046058 21.647979544944114 54-55 13.229044295487528 22.633197429341443 36.75713495957432 27.380623315596708 56-57 18.497339506599406 24.441987423122104 30.82544399143114 26.23522907884735 58-59 13.742139451316426 27.404809619238478 36.863381936286366 21.98966899315873 60-61 16.773201575564922 32.54612673623109 28.374853154585033 22.30581853361896 62-63 11.713081335083961 37.110427752055834 27.628532927924816 23.54795798493539 64-65 11.615472323958262 37.20026259415383 26.47104553935457 24.713219542533345 66-67 12.688307649782324 34.96302950729044 25.661668163914037 26.6869946790132 68-69 13.038370506020765 33.5970837724375 26.09142580723183 27.273119914309902 70-71 14.992053071660564 32.4891161633612 24.94212563057149 27.576705134406744 72-73 14.874576739686269 29.181639140349663 26.09011125699675 29.85367286296731 74-75 14.530785709349733 29.825167576532372 24.723585101236957 30.920461612880935 76-77 16.413862207172965 28.240964687996684 24.328830073940985 31.016343030889367 78-79 16.86821919701472 28.207276622209935 24.11115334116509 30.813350839610255 80-81 17.427095570451247 27.64148987630433 24.264045332043395 30.667369221201024 82-83 17.398590284016308 27.78488010503766 24.308098956533758 30.508430654412273 84-85 18.196738304194596 27.757238615161356 23.42961785640246 30.61640522424159 86-87 18.72797318775482 27.998237855020385 22.61592149816875 30.657867459056042 88-89 18.749568101720683 27.758102411719992 22.539043604450278 30.953285882109043 90-91 19.345587727178497 26.351841614263012 23.63606523391611 30.666505424642388 92-93 19.58745076359616 26.21277036832285 24.409163153893996 29.790615714187 94-95 19.270437426577292 25.421532720613644 25.491500241863037 29.81652961094603 96-97 18.13800013820745 25.54591942505701 26.69304125492364 29.6230391818119 98-99 18.877573056225003 25.90893778019643 25.737904584207932 29.475584579370633 100-101 18.3219888051966 24.746043811761453 27.226003731601132 29.705963651440815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 178.0 1 114.5 2 57.5 3 62.5 4 45.5 5 34.0 6 55.0 7 97.0 8 141.5 9 158.5 10 123.0 11 79.0 12 73.0 13 77.0 14 89.5 15 95.0 16 89.5 17 103.5 18 130.0 19 144.5 20 165.0 21 217.0 22 260.5 23 305.0 24 362.0 25 452.0 26 566.0 27 691.0 28 897.0 29 1093.0 30 1235.0 31 1439.5 32 1660.0 33 1818.5 34 1896.5 35 1915.0 36 1968.5 37 1942.0 38 1862.0 39 1769.0 40 1642.5 41 1544.0 42 1420.0 43 1398.0 44 1365.0 45 1304.0 46 1403.0 47 2052.0 48 3196.0 49 3434.0 50 3180.5 51 2758.0 52 1691.5 53 943.0 54 685.0 55 586.5 56 543.5 57 491.5 58 451.5 59 471.0 60 489.0 61 460.0 62 389.5 63 313.5 64 261.0 65 208.5 66 158.5 67 139.0 68 121.0 69 90.0 70 68.5 71 58.5 72 46.0 73 30.5 74 21.5 75 19.5 76 17.0 77 15.5 78 11.5 79 8.5 80 6.5 81 4.5 82 3.5 83 2.0 84 2.0 85 2.0 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11747633197429341 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.637965586345104E-4 44-45 0.0 46-47 8.637965586345104E-4 48-49 8.637965586345104E-4 50-51 8.637965586345104E-4 52-53 0.0017275931172690208 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0017275931172690208 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 8.637965586345104E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 57884.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.242761384838644 #Duplication Level Percentage of deduplicated Percentage of total 1 84.44379433920865 39.89358026397623 2 8.450961749433189 7.984935388017414 3 2.779199882981058 3.938912307373368 4 1.2872083668543846 2.432451109114781 5 0.6947999707452645 1.6412134614055698 6 0.45344840196006725 1.2853292792481514 7 0.29620419805456005 0.9795452974915347 8 0.22672420098003362 0.8568861861654344 9 0.17552841366196154 0.7463202266602169 >10 0.9654062751407884 8.46866146085274 >50 0.09873473268485336 3.4638242001243866 >100 0.10239157463614423 9.354916730011748 >500 0.00731368390258173 2.321885149609564 >1k 0.018284209756454325 16.631538939948864 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 4636 8.00912169165918 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 1570 2.712321194112363 TruSeq Adapter, Index 1 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 2.069656554488287 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 1134 1.9590905949830695 RNA PCR Primer, Index 1 (95% over 23bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1089 1.8813489047059637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 690 1.1920392509156243 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 654 1.1298458986939395 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 411 0.7100407711975676 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 398 0.6875820606730703 TruSeq Adapter, Index 1 (96% over 26bp) TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 336 0.580471287402391 TruSeq Adapter, Index 1 (96% over 28bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.5683781355815078 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 262 0.45262939672448343 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 209 0.36106696150922535 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 197 0.3403358441019971 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.3403358441019971 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 192 0.331697878515652 TruSeq Adapter, Index 1 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 192 0.331697878515652 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 192 0.331697878515652 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 188 0.3247875060465759 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.31787713357749986 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 183 0.3161495404602308 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 177 0.3057839817566167 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 171 0.29541842305300253 No Hit ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC 168 0.2902356437011955 Illumina PCR Primer Index 5 (95% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 152 0.26259415382489115 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 150 0.2591389675903531 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 146 0.25222859512127704 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.24013544330039388 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 134 0.23149747771404877 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 128 0.22113191901043466 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 123 0.21249395342408955 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 119 0.20558358095501347 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC 118 0.20385598783774447 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 113 0.19521802225139936 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 107 0.18485246354778523 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.1727593117269021 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 99 0.17103171860963307 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 99 0.17103171860963307 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 98 0.16930412549236404 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 94 0.16239375302328796 No Hit TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT 89 0.15375578743694285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 86 0.1485730080851358 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 81 0.13993504249879068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 80 0.13820744938152166 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 76 0.13129707691244558 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 0.12611429756063852 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 72 0.12438670444336951 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.12265911132610047 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.12265911132610047 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 70 0.12093151820883145 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT 69 0.11920392509156244 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.11747633197429341 No Hit CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA 68 0.11747633197429341 RNA PCR Primer, Index 1 (96% over 28bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 67 0.11574873885702439 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 66 0.11402114573975537 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 65 0.11229355262248635 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 64 0.11056595950521733 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 61 0.10538318015341026 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0017275931172690208 0.0 18-19 0.0 0.0 0.0 0.0034551862345380416 0.0 20-21 0.0 0.0 0.0 0.016412134614055697 0.0 22-23 0.0 0.0 0.0 0.07342270748393338 0.0 24-25 0.0 0.0 0.0 0.24445442609356643 0.0 26-27 0.0 0.0 0.0 0.4915002418630364 0.0 28-29 0.0 0.0 0.0 0.9674521456706517 0.0 30-31 0.0 0.0 0.0 1.540149264045332 0.0 32-33 0.0 0.0 0.0 2.1266671273581648 0.0 34-35 0.0 0.0 0.0 2.874714947135651 0.0 36-37 0.0 0.0 0.0 3.799841061433211 0.0 38-39 0.0 0.0 0.0 4.919321401423536 0.0 40-41 0.0 0.0 0.0 6.424918803123488 0.0 42-43 0.0 0.0 0.0 7.255891092529888 0.0 44-45 0.0 0.0 0.0 7.80612950038007 0.0 46-47 0.0 0.0 0.0 8.362414484140695 0.0 48-49 0.0 0.0 0.0 8.869463064059154 0.0 50-51 0.0 0.0 0.0 9.319501071107732 0.0 52-53 0.0 0.0 0.0 9.666747287678806 0.0 54-55 0.0 0.0 0.0 10.059774721857508 0.0 56-57 0.0 0.0 0.0 10.578052657038214 0.0 58-59 0.0 0.0 0.0 10.909750535553865 0.0 60-61 0.0 0.0 0.0 11.198258586137793 0.0 62-63 0.0 0.0 0.0 11.445304401907263 0.0 64-65 0.0 0.0 0.0 11.669891507152236 0.0 66-67 0.0 0.0 0.0 11.941987423122107 0.0 68-69 0.0 0.0 0.0 12.242588625526917 0.0 70-71 0.0 0.0 0.0 12.59933660424297 0.0 72-73 0.0 0.0 0.0 12.900801603206412 0.0 74-75 0.0 0.0 0.0 13.195356229700781 0.0 76-77 0.0 0.0 0.0 13.46140556976021 0.0 78-79 0.0 0.0 0.0 13.798286227627669 0.0 80-81 0.0 0.0 0.0 14.136894478612398 0.0 82-83 0.0 0.0 0.0 14.420219749844517 0.0 84-85 0.0 0.0 0.0 14.701817427959366 0.0 86-87 0.0 0.0 0.0 15.008465206274618 0.0 88-89 0.0 0.0 0.0 15.34189067790754 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 205 0.0 53.291714 9 GAGTAAA 50 0.0015897105 38.06511 1 CTTATAC 1555 0.0 35.494797 1 TATACAC 1560 0.0 35.01539 3 TTATACA 1595 0.0 34.544827 2 GTACCCA 60 0.0038875083 31.720922 1 ATGGGGC 60 0.0039206306 31.666092 5 TGCCGTT 30 0.00393676 31.666092 94-95 ATACACA 1760 0.0 31.036367 4 TACACAT 1770 0.0 30.861021 5 TTATAAC 40 4.4694552E-4 29.68696 68-69 ACCCATG 65 0.005799424 29.230238 3 ACACATC 1895 0.0 29.075989 6 CACATCT 1900 0.0 28.749475 7 GTACATA 50 4.9158392E-5 28.548832 1 CAGAAAA 245 0.0 28.111732 10-11 ACATCTC 1940 0.0 27.911863 8 GGAAAGC 35 0.008361702 27.142365 20-21 TGGGGCA 70 0.008327749 27.142365 6 CATAAGC 35 0.008361702 27.142365 4 >>END_MODULE