FastQCFastQC Report
Fri 10 Feb 2017
SRR2031857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885509
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97980.5196474798051879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85990.4560572238053491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52980.2809851345180532No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38500.063.292211
GTATCAA70000.041.3251
TCAACGC87550.032.281554
TATCAAC88600.032.2742652
ATCAACG87950.032.188743
CAACGCA89750.031.6490255
AACGCAG90300.031.508866
ACGCAGA100700.027.783027
CGCAGAG100400.027.4402398
GTACATG52950.027.1813031
TACATGG52500.026.5095232
GCAGAGT110800.024.5645319
ACATGGG55150.024.1160453
GAGTACT81300.023.4286612-13
CAGAGTA107300.022.75396210-11
AGAGTAC100050.022.19515210-11
CATGGGG32500.021.338464
GTACTTT90450.020.97982214-15
GTGGTAT14650.020.426621
AGTACTT86200.020.05800412-13
Produced by FastQC (version 0.11.5)