FastQCFastQC Report
Fri 10 Feb 2017
SRR2031857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885509
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117420.62274961296923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104770.55565897590518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65210.3458482563594234No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT28910.15332729782780138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.065.13381
GTATCAA81250.041.739951
TCAACGC100100.033.1687854
ATCAACG100600.033.0511443
TATCAAC101900.032.9557842
CAACGCA101200.032.620515
AACGCAG103100.032.11156
ACGCAGA113600.029.0180117
CGCAGAG113250.028.8560358
GTACATG53350.026.7468451
GCAGAGT123750.026.215739
TACATGG54200.025.5023462
GAGTACT93250.025.13765512-13
CAGAGTA120800.024.5360210-11
ACATGGG55350.024.0285113
GTGGTAT15200.023.7824041
AGAGTAC114800.022.57040410-11
GTACTTT104100.022.42638614-15
GTGTACG2509.3514245E-920.9285161
TGGTATC16900.020.7984732
Produced by FastQC (version 0.11.5)