FastQCFastQC Report
Fri 10 Feb 2017
SRR2031854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100455
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113880.36730092841212014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99910.3222430256204331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53090.17123293194063452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58250.056.510731
GTATCAA98650.039.386721
TCAACGC116500.033.1072964
ATCAACG118200.032.5105743
CAACGCA119200.032.397235
AACGCAG122550.031.8217076
TATCAAC122450.031.5761532
ACGCAGA135100.028.689867
CGCAGAG136300.028.3675738
GTACATG78500.024.445861
GCAGAGT154900.024.4092969
GAGTACT108800.023.7512-13
TACATGG78600.023.6895662
CAGAGTA147700.022.96208610-11
ACATGGG79750.022.692793
GTACTTT118700.021.5690814-15
CATGGGG48050.021.3527584
AGAGTAC138950.021.21176710-11
AGTACTT115300.019.75390412-13
GTGGTAT24600.018.7296751
Produced by FastQC (version 0.11.5)