FastQCFastQC Report
Fri 10 Feb 2017
SRR2031854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100455
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137770.4443541351188777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121350.3913941663401017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67410.2174197013019057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60150.058.6011621
GTATCAA105700.041.8534851
TCAACGC129300.034.0906564
ATCAACG129650.033.9986233
CAACGCA131600.033.4226575
TATCAAC134300.032.8214572
AACGCAG135300.032.613986
ACGCAGA148450.029.5649837
CGCAGAG149450.029.144688
GCAGAGT167150.025.6606259
GTACATG77850.023.5858961
GAGTACT126000.023.5422412-13
CAGAGTA165950.022.84080910-11
TACATGG80250.022.2550542
ACATGGG78350.021.8247573
GTACTTT135550.021.60326614-15
AGAGTAC155950.021.5489910-11
GTGGTAT26150.021.4651531
TGGTATC26400.020.3310782
AGTACTT132700.019.8300912-13
Produced by FastQC (version 0.11.5)