FastQCFastQC Report
Fri 10 Feb 2017
SRR2031853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911264
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68530.3585585246203559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57460.3006387395985065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.15743507961223568No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.054.1012081
GTATCAA57400.038.645471
TCAACGC65000.033.980774
CAACGCA66000.033.6098485
ATCAACG65300.033.460953
AACGCAG66950.033.1329356
TATCAAC68350.032.1068042
ACGCAGA76650.028.9399857
CGCAGAG77300.028.6351858
GCAGAGT87150.024.85379
GAGTACT61250.022.91632712-13
CAGAGTA84300.022.48220610-11
AGAGTAC78100.020.4049310-11
TACATGG42150.020.1720052
GTGGTAT16800.020.0744061
GTACATG43650.020.0229111
GTACTTT70000.020.01785714-15
TGGTATC16900.019.3934922
ACATGGG43000.018.558143
AGTACTT66150.017.9875312-13
Produced by FastQC (version 0.11.5)