##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031853_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1911264 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67013452877258 34.0 31.0 34.0 31.0 34.0 2 32.797543405829856 34.0 33.0 34.0 31.0 34.0 3 32.87415448624575 34.0 33.0 34.0 31.0 34.0 4 36.05483962445795 37.0 37.0 37.0 35.0 37.0 5 36.083915147253336 37.0 37.0 37.0 35.0 37.0 6 36.159825120967064 37.0 37.0 37.0 35.0 37.0 7 36.188365919098565 37.0 37.0 37.0 35.0 37.0 8 36.18198532489494 37.0 37.0 37.0 35.0 37.0 9 38.0313687695682 39.0 39.0 39.0 37.0 39.0 10-11 38.072260817971774 39.0 39.0 39.0 37.0 39.0 12-13 38.050512906641885 39.0 39.0 39.0 37.0 39.0 14-15 39.64616897508664 41.0 40.0 41.0 38.0 41.0 16-17 39.587288830847015 41.0 40.0 41.0 37.0 41.0 18-19 39.59999194250507 41.0 40.0 41.0 37.5 41.0 20-21 39.546046752306324 41.0 40.0 41.0 37.5 41.0 22-23 39.46416900020091 41.0 40.0 41.0 37.0 41.0 24-25 39.43405463609423 41.0 40.0 41.0 37.0 41.0 26-27 39.32226081797177 41.0 40.0 41.0 36.5 41.0 28-29 39.20302454292029 41.0 39.5 41.0 36.0 41.0 30-31 39.051667639844624 41.0 39.0 41.0 35.5 41.0 32-33 38.92305196979591 41.0 39.0 41.0 35.0 41.0 34-35 38.73103166281581 41.0 39.0 41.0 35.0 41.0 36-37 38.61846715053494 40.0 38.0 41.0 35.0 41.0 38-39 38.429875203007015 40.0 38.0 41.0 35.0 41.0 40-41 38.34358544921057 40.0 38.0 41.0 34.0 41.0 42-43 38.17904172317377 40.0 38.0 41.0 34.0 41.0 44-45 38.31048562626617 40.0 38.5 41.0 34.5 41.0 46-47 38.17436732968339 40.0 38.0 41.0 34.0 41.0 48-49 38.00219880665361 40.0 38.0 41.0 33.0 41.0 50-51 36.95123619761581 39.0 37.0 40.0 31.5 40.5 52-53 36.967401677633234 39.5 37.5 40.0 32.5 40.5 54-55 37.75004630443518 40.0 38.0 41.0 33.0 41.0 56-57 37.73936724596916 41.0 38.0 41.0 33.0 41.0 58-59 37.62752764662548 40.0 37.5 41.0 33.0 41.0 60-61 37.44395436737154 40.0 37.0 41.0 33.0 41.0 62-63 37.18722400463777 40.0 36.5 41.0 32.0 41.0 64-65 36.877523722520806 39.5 36.0 41.0 31.0 41.0 66-67 36.614025848862326 39.0 35.5 41.0 31.0 41.0 68-69 36.287574610310244 39.0 35.0 41.0 31.0 41.0 70-71 35.931778132168034 37.5 35.0 40.5 31.0 41.0 72-73 35.520767408374766 37.0 35.0 39.5 30.0 41.0 74-75 35.1481454158086 36.5 35.0 39.0 30.0 41.0 76-77 34.7568221867832 36.0 35.0 39.0 29.5 40.5 78-79 34.366954538985716 36.0 35.0 37.5 29.0 39.5 80-81 33.993871594923576 35.0 35.0 37.0 29.0 39.0 82-83 33.68541787005877 35.0 34.5 37.0 29.0 39.0 84-85 33.366467688398885 35.0 34.0 36.0 28.5 37.0 86-87 33.093138101277475 35.0 34.0 36.0 27.5 37.0 88-89 32.87945987576808 35.0 34.0 35.5 27.0 37.0 90-91 32.70751842759556 35.0 34.0 35.0 27.0 36.0 92-93 32.572686975739614 35.0 34.0 35.0 26.5 36.0 94-95 32.40773880531418 35.0 34.0 35.0 25.5 36.0 96-97 32.30273970524218 35.0 34.0 35.0 25.0 36.0 98-99 32.19218381134161 35.0 34.0 35.0 25.0 35.5 100-101 30.718405986823377 34.0 31.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36921320997129925 1101 2 0.25192221564100237 1101 3 0.24976477568385036 1101 4 0.21419519299075063 1101 5 0.25009025244639815 1101 6 0.27389942131534184 1101 7 0.22939892697363007 1101 8 0.33108313276083834 1101 9 0.3427602701366723 1101 10-11 0.31504254788224983 1101 12-13 0.3023735562833423 1101 14-15 0.36814701550376583 1101 16-17 0.4286316897092135 1101 18-19 0.40181731552662825 1101 20-21 0.36609843418373345 1101 22-23 0.42325156496831795 1101 24-25 0.40149423491126157 1101 26-27 0.3180248537706447 1101 28-29 0.39708910164162603 1101 30-31 0.40268624528195573 1101 32-33 0.24896372764593622 1101 34-35 0.38505476233877545 1101 36-37 0.28751464059090637 1101 38-39 0.4983401399169196 1101 40-41 0.4328800892959137 1101 42-43 0.41177837267959205 1101 44-45 0.4659689476503033 1101 46-47 0.704772235743583 1101 48-49 0.5790581793945009 1101 50-51 0.5545768847728212 1101 52-53 0.5952040219756682 1101 54-55 0.6158941089339365 1101 56-57 0.4624267693516515 1101 58-59 0.5663549290950343 1101 60-61 0.5490701923038586 1101 62-63 0.5269879308263228 1101 64-65 0.6599928227710379 1101 66-67 0.5711329240718896 1101 68-69 0.5421163525304422 1101 70-71 0.5855121203633402 1101 72-73 0.5695681857033605 1101 74-75 0.6432699831932354 1101 76-77 0.5957480971115316 1101 78-79 0.5338734514938679 1101 80-81 0.5921855243037939 1101 82-83 0.5574365036787157 1101 84-85 0.5190779949183835 1101 86-87 0.5749232014364623 1101 88-89 0.5200835463309375 1101 90-91 0.4822800720268816 1101 92-93 0.3444503449872869 1101 94-95 0.4214620654450698 1101 96-97 0.350535481139552 1101 98-99 0.43971949314728676 1101 100-101 0.3620620117037845 1102 1 0.09456718207734127 1102 2 0.10227177308127011 1102 3 0.13158795696768522 1102 4 0.15595860390448024 1102 5 0.10946843205686463 1102 6 0.1277723024859938 1102 7 0.10796576669519453 1102 8 0.09247423178557312 1102 9 0.15293132371978402 1102 10-11 0.22033577418726935 1102 12-13 0.2496691740758763 1102 14-15 0.3171555236158241 1102 16-17 0.2817162377062985 1102 18-19 0.2661626247970901 1102 20-21 0.2893177261890614 1102 22-23 0.3340547146011019 1102 24-25 0.3133400865549518 1102 26-27 0.3325879952636228 1102 28-29 0.3640095905679601 1102 30-31 0.2834938432160641 1102 32-33 0.2912385670782385 1102 34-35 0.4927332956270405 1102 36-37 0.4703007235462593 1102 38-39 0.4478092448245121 1102 40-41 0.4481283448722664 1102 42-43 0.365439307848753 1102 44-45 0.39364095564211965 1102 46-47 0.4590035877749088 1102 48-49 0.4036126345070272 1102 50-51 0.414930915741067 1102 52-53 0.4371121077934035 1102 54-55 0.5412494685063649 1102 56-57 0.5736210583718346 1102 58-59 0.4697288269001447 1102 60-61 0.4860214073039728 1102 62-63 0.40330055811585197 1102 64-65 0.5371291005603069 1102 66-67 0.5054212547227834 1102 68-69 0.43890945522483094 1102 70-71 0.42838491576333837 1102 72-73 0.4161064150271585 1102 74-75 0.4604445886902937 1102 76-77 0.3630422349399254 1102 78-79 0.3531595285630047 1102 80-81 0.42304811636008566 1102 82-83 0.303606045343912 1102 84-85 0.2801295851230421 1102 86-87 0.47957819320416206 1102 88-89 0.49953853271694726 1102 90-91 0.42939027385698836 1102 92-93 0.40075331046543994 1102 94-95 0.528720154374092 1102 96-97 0.4300656850214395 1102 98-99 0.45574451967732443 1102 100-101 0.24957401539024104 1103 1 0.19009603616711956 1103 2 0.2123156560889825 1103 3 0.2565003622485946 1103 4 0.24235424861718258 1103 5 0.20224097231928795 1103 6 0.23180524121485035 1103 7 0.26238396646409967 1103 8 0.32385042657754326 1103 9 0.32864555159374476 1103 10-11 0.26484479216092893 1103 12-13 0.2892985822314529 1103 14-15 0.3127750090416228 1103 16-17 0.34565882540223924 1103 18-19 0.34910119792972694 1103 20-21 0.35980616991510317 1103 22-23 0.2979501179416886 1103 24-25 0.2724827950926709 1103 26-27 0.38151762672734435 1103 28-29 0.36285762268447286 1103 30-31 0.46678676618564907 1103 32-33 0.36692507608998426 1103 34-35 0.4505667550967729 1103 36-37 0.4305981251365125 1103 38-39 0.40663855892282186 1103 40-41 0.3722759898590411 1103 42-43 0.27737547037471444 1103 44-45 0.4176664351940005 1103 46-47 0.40210283687405024 1103 48-49 0.4186703613734508 1103 50-51 0.32276725610378065 1103 52-53 0.46486483997002637 1103 54-55 0.467777743303067 1103 56-57 0.38945919578887356 1103 58-59 0.37293393713712675 1103 60-61 0.4380798442715914 1103 62-63 0.5596018496902531 1103 64-65 0.6073025926074891 1103 66-67 0.6722039357285112 1103 68-69 0.5487477826250711 1103 70-71 0.5088677222825666 1103 72-73 0.5677605147714644 1103 74-75 0.5741966481394698 1103 76-77 0.47465659393345305 1103 78-79 0.45765457809878995 1103 80-81 0.413364033527408 1103 82-83 0.44519142411125756 1103 84-85 0.4633360813031331 1103 86-87 0.4234724130427736 1103 88-89 0.24898104099367657 1103 90-91 0.1836080569086178 1103 92-93 0.25149969533405425 1103 94-95 0.348668625320677 1103 96-97 0.42636685201667746 1103 98-99 0.35055649858607296 1103 100-101 0.414366155068393 1104 1 0.1719187712078778 1104 2 0.1999270853862285 1104 3 0.2461007756339697 1104 4 0.29472096647589296 1104 5 0.25226363345875313 1104 6 0.27221784715928976 1104 7 0.21447442049518628 1104 8 0.30540902222198696 1104 9 0.3558995411090038 1104 10-11 0.31966093382047234 1104 12-13 0.31895508554546126 1104 14-15 0.34055439058015224 1104 16-17 0.38369995031852255 1104 18-19 0.37497350746068037 1104 20-21 0.3369923925196616 1104 22-23 0.37297581018898285 1104 24-25 0.37346884333636154 1104 26-27 0.4749241011736558 1104 28-29 0.4614361052830276 1104 30-31 0.5125629895985071 1104 32-33 0.4798255431692624 1104 34-35 0.4934406927656454 1104 36-37 0.5636096317991743 1104 38-39 0.5329827109054577 1104 40-41 0.43935151929254346 1104 42-43 0.5197479040367554 1104 44-45 0.523366938646582 1104 46-47 0.5265438861569791 1104 48-49 0.5139950940586075 1104 50-51 0.44754244893997 1104 52-53 0.4920625374827807 1104 54-55 0.5290999414047306 1104 56-57 0.6123351108947617 1104 58-59 0.5022608435596112 1104 60-61 0.44494061249865524 1104 62-63 0.42823724494671467 1104 64-65 0.5623808529247967 1104 66-67 0.44029495845315836 1104 68-69 0.46806727005031235 1104 70-71 0.48264070096666245 1104 72-73 0.43149886665710113 1104 74-75 0.36447862777041706 1104 76-77 0.36820480875527295 1104 78-79 0.36258748957889253 1104 80-81 0.45509553222259314 1104 82-83 0.43933777754420333 1104 84-85 0.46510531007383094 1104 86-87 0.4599121026573272 1104 88-89 0.5102567621086607 1104 90-91 0.4519632190295866 1104 92-93 0.5226514152712411 1104 94-95 0.45477652409899605 1104 96-97 0.40701864123632703 1104 98-99 0.5201581677161187 1104 100-101 0.3855060392512044 1105 1 -0.017331314652984986 1105 2 0.014919733549881187 1105 3 0.0754563361980729 1105 4 0.05101631174388643 1105 5 1.2314898884113745E-4 1105 6 0.05949554802004542 1105 7 0.10794188459461651 1105 8 0.14207952579594263 1105 9 0.2600707691339892 1105 10-11 0.16071302453246972 1105 12-13 0.16051295040046654 1105 14-15 0.19942328104315976 1105 16-17 0.24101462501317883 1105 18-19 0.25880476671756014 1105 20-21 0.2859242968062645 1105 22-23 0.28372210207299275 1105 24-25 0.26113164358106644 1105 26-27 0.2537217002892902 1105 28-29 0.23537082687917632 1105 30-31 0.2724027785954348 1105 32-33 0.2369241570529681 1105 34-35 0.2585465028628846 1105 36-37 0.2065793712114612 1105 38-39 0.20928893635461066 1105 40-41 0.15727041297532907 1105 42-43 0.16083809550648454 1105 44-45 0.10767559771456092 1105 46-47 0.26023937792506757 1105 48-49 0.19673719485926 1105 50-51 0.12466569415859396 1105 52-53 0.08247268409019881 1105 54-55 0.10970316762663401 1105 56-57 0.16619133688055143 1105 58-59 0.2430572969693614 1105 60-61 0.28152223666418763 1105 62-63 0.26404511516802387 1105 64-65 0.3240975067123415 1105 66-67 0.2648460000527493 1105 68-69 0.1646930052339428 1105 70-71 0.20911683701319816 1105 72-73 0.31707819941087223 1105 74-75 0.14822194672206024 1105 76-77 0.304528917412199 1105 78-79 0.2796297975188722 1105 80-81 0.2640769138311967 1105 82-83 0.236513625956853 1105 84-85 0.17579411542725154 1105 86-87 0.2870124740055573 1105 88-89 0.23091917866263856 1105 90-91 0.22983144138775913 1105 92-93 0.18366501616848296 1105 94-95 0.07956623206474234 1105 96-97 0.19677121437904788 1105 98-99 0.08536244949000604 1105 100-101 0.21448361078403977 1106 1 0.1729420350895765 1106 2 0.13487442710070496 1106 3 0.19655305697522607 1106 4 0.1516795492068752 1106 5 0.2038310847356044 1106 6 0.1663815538469393 1106 7 0.16320313971313283 1106 8 0.1608958136392502 1106 9 0.19081296819513938 1106 10-11 0.17382479584230737 1106 12-13 0.1903105574506938 1106 14-15 0.24874930390367922 1106 16-17 0.29933731232999605 1106 18-19 0.2498424442500351 1106 20-21 0.290659120457029 1106 22-23 0.3238060713575166 1106 24-25 0.277317959484904 1106 26-27 0.30345264641732683 1106 28-29 0.3819836453074572 1106 30-31 0.4543107320751574 1106 32-33 0.4115241353238517 1106 34-35 0.48195081796499295 1106 36-37 0.47014570557255553 1106 38-39 0.49036063118795425 1106 40-41 0.3960171676573978 1106 42-43 0.46737375060801156 1106 44-45 0.5228892860566461 1106 46-47 0.5502100186466734 1106 48-49 0.4853650638202254 1106 50-51 0.3988739864376427 1106 52-53 0.5166506430271767 1106 54-55 0.5143807338161324 1106 56-57 0.4328671822242143 1106 58-59 0.3772682866555286 1106 60-61 0.4578608561661923 1106 62-63 0.523374896915314 1106 64-65 0.6036447294444827 1106 66-67 0.6057074095228998 1106 68-69 0.5988007191939033 1106 70-71 0.6208641698894652 1106 72-73 0.6247808577305705 1106 74-75 0.5551979285595152 1106 76-77 0.5445201008870839 1106 78-79 0.6064103671962755 1106 80-81 0.5686347825798563 1106 82-83 0.46295439726543464 1106 84-85 0.5688397378227208 1106 86-87 0.5462701375764567 1106 88-89 0.500620865416245 1106 90-91 0.45583201084036773 1106 92-93 0.5011560778854118 1106 94-95 0.5140070633157023 1106 96-97 0.5649407489139762 1106 98-99 0.48844248880222096 1106 100-101 0.537476259100842 1107 1 0.16290596797337287 1107 2 0.23067356636855862 1107 3 0.22010122487026962 1107 4 0.23634957284506442 1107 5 0.28630513790120204 1107 6 0.22486023010827694 1107 7 0.21381616453032848 1107 8 0.21305664626111565 1107 9 0.15864237828579775 1107 10-11 0.21876058940597432 1107 12-13 0.20897892002684415 1107 14-15 0.20931998906272753 1107 16-17 0.30558836501354136 1107 18-19 0.3181841119330784 1107 20-21 0.3402789927991847 1107 22-23 0.3296906449698582 1107 24-25 0.35717061282822726 1107 26-27 0.3152336998558951 1107 28-29 0.3014171999221418 1107 30-31 0.3223332551470719 1107 32-33 0.21094857152106528 1107 34-35 0.2720024502778742 1107 36-37 0.33431380969674507 1107 38-39 0.33861157932913244 1107 40-41 0.27224684723504566 1107 42-43 0.2317131880539236 1107 44-45 0.2921213062217092 1107 46-47 0.25976896602120547 1107 48-49 0.3153396832569655 1107 50-51 0.25515669222638593 1107 52-53 0.1978788996670673 1107 54-55 0.3032925639464068 1107 56-57 0.292833264034428 1107 58-59 0.22631027203120624 1107 60-61 0.20796191378646256 1107 62-63 0.18014837103236658 1107 64-65 0.22388442185761193 1107 66-67 0.20549193609117822 1107 68-69 0.2532234420579016 1107 70-71 0.20785096851183482 1107 72-73 0.21557125609506045 1107 74-75 0.18292227357255086 1107 76-77 0.12677461508452126 1107 78-79 0.11982518549583432 1107 80-81 0.19373453342055313 1107 82-83 0.2567675219786665 1107 84-85 0.20085235260152956 1107 86-87 0.19015818431850562 1107 88-89 0.17091863692147768 1107 90-91 0.19388601278021156 1107 92-93 0.1919807429142466 1107 94-95 0.20306786631723384 1107 96-97 0.1898288014639391 1107 98-99 0.22344414405634438 1107 100-101 0.26902249877002404 1108 1 0.1389237325921755 1108 2 0.10060530417594293 1108 3 0.09742666281746892 1108 4 0.17324953720617486 1108 5 0.12012644996085697 1108 6 0.20043113780353394 1108 7 0.22939584487684783 1108 8 0.22236388095695503 1108 9 0.2487018678271511 1108 10-11 0.15942594017495537 1108 12-13 0.11558171082652535 1108 14-15 0.17393741922420247 1108 16-17 0.1561489922581032 1108 18-19 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-0.37023546360762083 2316 90-91 -0.3673683963908658 2316 92-93 -0.6882551477383103 2316 94-95 -0.7784301495557884 2316 96-97 -0.6211886147618095 2316 98-99 -0.7428863233157941 2316 100-101 -0.6439459756567842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 14.0 7 356.0 8 1007.0 9 847.0 10 945.0 11 1201.0 12 1634.0 13 1913.0 14 2013.0 15 2154.0 16 2591.0 17 3587.0 18 5063.0 19 7019.0 20 9595.0 21 12508.0 22 14538.0 23 14003.0 24 12732.0 25 11345.0 26 10872.0 27 10947.0 28 12331.0 29 14179.0 30 16811.0 31 20682.0 32 26010.0 33 35093.0 34 51310.0 35 84216.0 36 161641.0 37 386479.0 38 708571.0 39 266749.0 40 308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90338749644215 19.75629740318449 13.96871389823698 23.37160120213639 2 15.094408726371658 21.236155758702093 40.63473177959717 23.03470373532908 3 17.80926130560718 25.851740000334857 31.54451713630351 24.794481557754448 4 12.137569692099051 18.27115458670283 39.72632770773687 29.86494801346125 5 12.737068243842817 38.113782292765414 36.12478443584978 13.02436502754198 6 29.63028655381988 38.84010790764646 18.41022485643009 13.119380682103571 7 25.504378254390815 33.965794364357826 23.027797311098833 17.50203007015253 8 24.337349523666013 35.56614889413498 21.554269844458954 18.54223173774005 9 25.434215262778974 15.915279103253136 21.48646131565289 37.164044318315 10-11 23.16056285264621 26.75810353776349 30.463007726823715 19.618325882766587 12-13 24.38443877978134 24.991262326920825 29.5155195723877 21.10877932091014 14-15 21.761907303229695 26.722708113583472 27.510799136069114 24.00458544711772 16-17 20.918931136671855 29.52334162104241 28.168897650978618 21.388829591307115 18-19 20.62865203341872 28.66404117903126 30.18021058315335 20.52709620439667 20-21 21.677617534783263 27.865067306243407 30.222957163426926 20.234357995546404 22-23 21.310575222628003 27.91323524795161 29.784697006163473 20.99149252325691 24-25 21.148361958842365 27.916954325716286 29.99720341774268 20.937480297698666 26-27 21.19500498099687 28.128113123043182 29.802842516784704 20.874039379175247 28-29 21.15395884608301 28.24693501264085 29.80629572889983 20.792810412376312 30-31 21.4198300182497 27.99610624173322 29.829604910676917 20.754458829340162 32-33 21.11471779932024 28.126334195589937 29.76859816330973 20.990349841780098 34-35 21.458129279890166 28.25588197130276 29.547487945150436 20.73850080365664 36-37 21.44499660957356 28.095019840273245 29.494512532020696 20.965471018132504 38-39 21.278980821069197 28.24073492725233 29.584897743064275 20.895386508614195 40-41 21.499175414804025 28.118093575769755 29.486376554991878 20.896354454434345 42-43 21.36868585396889 28.240839570043697 29.296763817034172 21.09371075895324 44-45 21.536009677365346 28.448712475095018 28.98398651363705 21.03129133390259 46-47 21.498599879451504 28.287954986856867 28.85271213186666 21.36073300182497 48-49 21.42359715873893 28.349432626785205 28.915026914125942 21.311943300349924 50-51 21.541424941818608 28.567403561203474 28.664590553685937 21.226580943291978 52-53 21.538835032732266 28.630817092772116 28.49295021514558 21.337397659350042 54-55 21.520784151221388 28.55076535737606 28.587338012958963 21.341112478443584 56-57 21.502680948314833 28.575277931253872 28.506082885462185 21.41595823496911 58-59 21.408423953990656 28.475474868987227 28.63288378790162 21.483217389120497 60-61 21.53109146617108 28.38163644582852 28.690332680362314 21.396939407638087 62-63 21.38537637919199 28.45857505818139 28.70908990071492 21.446958661911697 64-65 21.359712734609136 28.347575217238436 28.68489125521121 21.607820792941215 66-67 21.274507341738243 28.40340214643294 28.582916855023694 21.73917365680513 68-69 21.309981248011788 28.424461508195627 28.558038031376093 21.707519212416496 70-71 21.276966447335376 28.192939332295275 28.65739636177943 21.872697858589916 72-73 21.261897885378474 28.147681325028884 28.649155741959248 21.941265047633397 74-75 21.206541848745122 28.292663912468395 28.532191261908352 21.96860297687813 76-77 21.131356003147655 28.215437532439264 28.60839737472165 22.04480908969143 78-79 21.107968339277043 28.16180810186348 28.64578101193765 22.084442546921828 80-81 21.101192718536005 28.1776353240578 28.55599749694443 22.165174460461767 82-83 21.106294054615166 28.169237740050562 28.57145846936896 22.15300973596531 84-85 21.178340616471615 27.96738179550287 28.57182471913875 22.282452868886768 86-87 21.089629690089907 28.012849088352006 28.576141234282655 22.321379987275435 88-89 21.11189244395332 27.866689269509603 28.632517538131836 22.388900748405245 90-91 21.132794841528955 27.799613240243104 28.663387161585213 22.404204756642724 92-93 21.000186264168633 27.727383553501767 28.787310387262043 22.485119795067558 94-95 20.90692337636245 27.64223048202655 28.807872695765735 22.642973445845264 96-97 20.86074974467159 27.543186079997323 28.83782669479465 22.75823748053644 98-99 20.919585154117904 27.479667905637317 28.737735864851743 22.86301107539304 100-101 20.981350561722504 27.34483566896044 28.881070328327223 22.792743440989838 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 346.0 1 241.5 2 173.0 3 236.0 4 237.0 5 240.0 6 365.0 7 695.5 8 1290.5 9 1742.5 10 1664.0 11 1496.0 12 1599.5 13 1805.5 14 1934.0 15 2069.5 16 2287.5 17 2506.5 18 2763.5 19 3116.5 20 3570.5 21 4279.5 22 5267.5 23 6481.5 24 8202.0 25 10469.0 26 13186.0 27 16326.5 28 19679.5 29 23626.0 30 28121.0 31 32378.5 32 37030.5 33 41646.0 34 46093.0 35 49935.5 36 53872.5 37 58658.0 38 62683.0 39 66077.5 40 68947.0 41 70906.5 42 72994.0 43 75889.0 44 77872.0 45 78939.5 46 78996.0 47 78350.0 48 77186.5 49 75072.0 50 71703.5 51 66671.5 52 61637.5 53 56996.5 54 51286.5 55 44253.0 56 37339.5 57 31719.0 58 27073.0 59 22214.5 60 17388.0 61 13598.5 62 10522.0 63 7723.0 64 5460.0 65 3893.5 66 2884.0 67 2249.5 68 1795.0 69 1372.0 70 1038.0 71 794.0 72 600.5 73 439.5 74 323.5 75 240.0 76 174.0 77 117.5 78 89.5 79 61.0 80 49.0 81 42.0 82 31.0 83 23.0 84 19.5 85 14.0 86 9.5 87 7.5 88 6.5 89 8.5 90 8.5 91 5.5 92 2.5 93 1.5 94 1.0 95 2.0 96 1.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.0928558273477658E-4 24-25 1.3080348920923535E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1911264.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.09568582413834 #Duplication Level Percentage of deduplicated Percentage of total 1 70.29605421567095 42.94785643037228 2 16.956071312555355 20.718856114471347 3 6.274237886128087 11.499866000303625 4 2.776483365824895 6.7852462165753575 5 1.4339601207598571 4.380438851114386 6 0.7757388337110656 2.8436577639596856 7 0.45916819939021136 1.9637237235265768 8 0.2986691338825389 1.4597916455244089 9 0.19313115511225254 1.0619532338031998 >10 0.5237907389052935 4.621926954452871 >50 0.00856253916994896 0.3525760511245025 >100 0.0037022643560119615 0.4639872466245208 >500 1.7209381345319373E-4 0.07959241496173747 >1k 8.604690672659686E-5 0.1581859819153686 >5k 1.7209381345319373E-4 0.6623413712700994 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6853 0.3585585246203559 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5746 0.3006387395985065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3009 0.15743507961223568 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.232139568369414E-5 0.0 12-13 0.0 0.0 0.0 5.232139568369414E-5 0.0 14-15 0.0 0.0 0.0 5.232139568369414E-5 0.0 16-17 0.0 0.0 0.0 5.232139568369414E-5 0.0 18-19 0.0 0.0 0.0 7.84820935255412E-5 0.0 20-21 0.0 0.0 0.0 1.569641870510824E-4 2.616069784184707E-5 22-23 0.0 0.0 0.0 4.1857116546955317E-4 5.232139568369414E-5 24-25 0.0 0.0 0.0 0.001412677683459742 5.232139568369414E-5 26-27 0.0 0.0 0.0 0.0035840156043330487 5.232139568369414E-5 28-29 0.0 0.0 0.0 0.008816155172702463 5.232139568369414E-5 30-31 0.0 0.0 0.0 0.021137843856212434 5.232139568369414E-5 32-33 0.0 0.0 0.0 0.04211872352537378 5.232139568369414E-5 34-35 0.0 0.0 0.0 0.07544745257588695 5.232139568369414E-5 36-37 0.0 0.0 0.0 0.13041107874160765 5.232139568369414E-5 38-39 0.0 0.0 0.0 0.20350406851172836 5.232139568369414E-5 40-41 0.0 0.0 0.0 0.28154143017395816 5.232139568369414E-5 42-43 0.0 0.0 0.0 0.35513147320307403 5.232139568369414E-5 44-45 0.0 0.0 0.0 0.45135051986538754 5.232139568369414E-5 46-47 0.0 0.0 0.0 0.5693091064342759 5.232139568369414E-5 48-49 0.0 0.0 0.0 0.6911656369815996 7.84820935255412E-5 50-51 0.0 0.0 0.0 0.8156120766152661 1.0464279136738828E-4 52-53 0.0 0.0 0.0 0.9354803941266094 1.0464279136738828E-4 54-55 0.0 0.0 0.0 1.0736088787315619 1.0464279136738828E-4 56-57 0.0 0.0 0.0 1.2460601989050177 1.0464279136738828E-4 58-59 0.0 0.0 0.0 1.4390738275821655 1.0464279136738828E-4 60-61 0.0 0.0 0.0 1.6452201265759205 1.0464279136738828E-4 62-63 0.0 0.0 0.0 1.8552382088502688 1.0464279136738828E-4 64-65 0.0 0.0 0.0 2.0798539605203676 1.0464279136738828E-4 66-67 0.0 0.0 0.0 2.351454325514424 1.0464279136738828E-4 68-69 0.0 0.0 0.0 2.668600465451136 1.0464279136738828E-4 70-71 0.0 0.0 0.0 3.0037974868987227 1.0464279136738828E-4 72-73 0.0 0.0 0.0 3.347130485375123 1.0464279136738828E-4 74-75 0.0 0.0 0.0 3.6981285683191856 1.0464279136738828E-4 76-77 0.0 0.0 0.0 4.082350737522393 1.0464279136738828E-4 78-79 0.0 0.0 0.0 4.519495998459658 1.0464279136738828E-4 80-81 0.0 0.0 0.0 4.998655340130929 1.0464279136738828E-4 82-83 0.0 0.0 0.0 5.499318775428199 1.0464279136738828E-4 84-85 0.0 0.0 0.0 6.017562199675189 1.0464279136738828E-4 86-87 0.0 0.0 0.0 6.57222131531803 1.0464279136738828E-4 88-89 0.0 0.0 0.0 7.217370284795821 1.0464279136738828E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3310 0.0 54.101208 1 GTATCAA 5740 0.0 38.64547 1 TCAACGC 6500 0.0 33.98077 4 CAACGCA 6600 0.0 33.609848 5 ATCAACG 6530 0.0 33.46095 3 AACGCAG 6695 0.0 33.132935 6 TATCAAC 6835 0.0 32.106804 2 ACGCAGA 7665 0.0 28.939985 7 CGCAGAG 7730 0.0 28.635185 8 GCAGAGT 8715 0.0 24.8537 9 GAGTACT 6125 0.0 22.916327 12-13 CAGAGTA 8430 0.0 22.482206 10-11 AGAGTAC 7810 0.0 20.40493 10-11 TACATGG 4215 0.0 20.172005 2 GTGGTAT 1680 0.0 20.074406 1 GTACATG 4365 0.0 20.022911 1 GTACTTT 7000 0.0 20.017857 14-15 TGGTATC 1690 0.0 19.393492 2 ACATGGG 4300 0.0 18.55814 3 AGTACTT 6615 0.0 17.98753 12-13 >>END_MODULE