FastQCFastQC Report
Fri 10 Feb 2017
SRR2031853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911264
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85460.44713864751285015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72090.37718494148375104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37850.19803648266278231No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT32520.17014917876337335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34050.057.278221
GTATCAA61350.040.784391
TCAACGC72200.034.6704524
ATCAACG72350.034.4016153
CAACGCA72850.034.03515
AACGCAG75200.032.9715046
TATCAAC75200.032.8451772
ACGCAGA85250.029.1402577
CGCAGAG85300.029.1231778
CAACGCG700.00839364727.1423855
GCAGAGT95700.025.6604739
GAGTACT75000.023.4962612-13
GTGGTAT14750.023.2200551
CAGAGTA91700.023.05030810-11
AGAGTAC87450.020.85727110-11
GTACTTT84350.020.80728514-15
GTACATG37650.020.7205681
TACATGG35700.019.6915362
TGGTATC15450.019.3685972
AGTACTT79900.019.08289712-13
Produced by FastQC (version 0.11.5)