FastQCFastQC Report
Fri 10 Feb 2017
SRR2031852_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031852_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534190
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC7860.1471386585297366No Hit
CCATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT7640.14302027368539283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.13309871019674646No Hit
ATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGC5870.10988599561953612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.038.255031
GTATCAA10650.036.1267621
GTATAAG1001.297467E-633.2500041
GTATAAT600.00394907531.6666681
TTTATAT1102.7298393E-630.2272723
TATCAAC12600.029.7817462
TCAACGC12850.028.4630364
ATCAACG13250.027.9622633
CAACGCA13100.027.9198475
AACGCAG13100.027.9198476
GTACATG14300.027.90211
GTTCATA1205.3746044E-627.7083341
TACATGG14100.025.9397162
ATAATGC1301.0007978E-525.5769233
ACGCAGA15100.024.2218537
TATAAGG1201.7091463E-423.752
CGCAGAG15600.023.4455138
GTATAAA1252.2537084E-422.81
TATAAGA1050.002194693522.6190472
TATACAG1302.9386563E-421.9230775
Produced by FastQC (version 0.11.5)