##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031852_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 534190 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26972425541474 34.0 31.0 34.0 31.0 34.0 2 32.407980306632474 34.0 31.0 34.0 31.0 34.0 3 32.48642992193789 34.0 31.0 34.0 31.0 34.0 4 35.624262902712516 37.0 37.0 37.0 35.0 37.0 5 35.63447462513338 37.0 37.0 37.0 35.0 37.0 6 35.7106235609053 37.0 37.0 37.0 35.0 37.0 7 35.732252569310546 37.0 37.0 37.0 35.0 37.0 8 35.726576686197795 37.0 37.0 37.0 35.0 37.0 9 37.54850895748704 39.0 39.0 39.0 35.0 39.0 10-11 37.57126022576237 39.0 39.0 39.0 35.0 39.0 12-13 37.55005803178644 39.0 39.0 39.0 35.0 39.0 14-15 39.10931690971377 41.0 40.0 41.0 36.0 41.0 16-17 39.031010501881354 41.0 40.0 41.0 36.0 41.0 18-19 39.00158745015818 41.0 40.0 41.0 36.0 41.0 20-21 38.934486793088595 41.0 40.0 41.0 36.0 41.0 22-23 38.832697167674425 41.0 39.0 41.0 35.5 41.0 24-25 38.78720118309965 41.0 39.0 41.0 35.0 41.0 26-27 38.65361107471124 41.0 39.0 41.0 35.0 41.0 28-29 38.51452760253842 41.0 39.0 41.0 35.0 41.0 30-31 38.38252868829443 40.5 38.5 41.0 34.0 41.0 32-33 38.290757970010674 40.0 38.0 41.0 34.0 41.0 34-35 38.07778412175443 40.0 38.0 41.0 33.5 41.0 36-37 37.964243995582095 40.0 38.0 41.0 33.0 41.0 38-39 37.780872910387686 40.0 38.0 41.0 33.0 41.0 40-41 37.665344727531405 40.0 38.0 41.0 33.0 41.0 42-43 37.478817461951735 40.0 38.0 41.0 32.0 41.0 44-45 37.70388625769857 40.0 38.0 41.0 33.0 41.0 46-47 37.62759973043299 40.0 38.0 41.0 32.5 41.0 48-49 37.458753439787344 40.0 37.5 41.0 32.0 41.0 50-51 36.3438345906887 39.0 36.0 40.0 30.5 40.5 52-53 36.33286658305097 39.0 36.0 39.5 30.5 40.5 54-55 37.01289335255247 40.0 36.0 41.0 31.0 41.0 56-57 36.911245998614724 39.5 36.0 41.0 31.0 41.0 58-59 36.68230592111421 39.0 35.0 41.0 31.0 41.0 60-61 36.35519103689698 39.0 35.0 41.0 31.0 41.0 62-63 35.9667150264887 38.0 35.0 41.0 30.0 41.0 64-65 35.53092158220858 37.0 35.0 40.0 29.0 41.0 66-67 35.16515472023063 36.5 35.0 39.0 29.0 41.0 68-69 34.75452273535633 36.0 35.0 39.0 29.0 41.0 70-71 34.33737808644864 35.5 35.0 38.0 29.0 40.0 72-73 33.93192684250922 35.0 35.0 37.0 28.0 39.0 74-75 33.58192403451955 35.0 34.0 37.0 28.0 39.0 76-77 33.26813586926 35.0 34.0 36.0 27.0 37.5 78-79 32.98677343267377 35.0 34.0 36.0 27.0 37.0 80-81 32.748188846665045 35.0 34.0 35.0 26.0 36.5 82-83 32.571581272580914 35.0 34.0 35.0 26.0 36.0 84-85 32.38069413504559 35.0 34.0 35.0 25.5 36.0 86-87 32.23200172223366 35.0 34.0 35.0 25.0 35.5 88-89 32.11455568243509 35.0 34.0 35.0 25.0 35.0 90-91 32.02159531252925 35.0 33.0 35.0 24.5 35.0 92-93 31.935407813699243 35.0 33.0 35.0 24.0 35.0 94-95 31.811923660120932 35.0 33.0 35.0 23.5 35.0 96-97 31.74307736947528 35.0 33.0 35.0 23.0 35.0 98-99 31.632307793107323 35.0 33.0 35.0 23.0 35.0 100-101 29.942556019393848 33.5 29.0 34.5 15.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3899132121314892 1101 2 0.3399655959684935 1101 3 0.4680587835189627 1101 4 0.39457541759816905 1101 5 0.4475999296968709 1101 6 0.5061277452506658 1101 7 0.5333377319385875 1101 8 0.5757342021062897 1101 9 0.5474240848603458 1101 10-11 0.6325307170876542 1101 12-13 0.7291223877403183 1101 14-15 0.6392337758700535 1101 16-17 0.6392577659820873 1101 18-19 0.63711100471847 1101 20-21 0.5544387898536698 1101 22-23 0.7327127512851561 1101 24-25 0.459816351869641 1101 26-27 0.5460595016734544 1101 28-29 0.5979954474730462 1101 30-31 0.390251158755369 1101 32-33 0.25506382128750005 1101 34-35 0.5329635757018778 1101 36-37 0.6529544426578795 1101 38-39 0.5539956870503531 1101 40-41 0.5248943216925213 1101 42-43 0.5584714418137722 1101 44-45 0.5650818675251301 1101 46-47 0.6182569618976501 1101 48-49 0.6855726150338981 1101 50-51 0.5128229579169457 1101 52-53 0.37435978326020347 1101 54-55 0.5692626578611737 1101 56-57 0.5316910806718909 1101 58-59 0.4327961565877416 1101 60-61 0.5067976459268877 1101 62-63 0.5618161427045081 1101 64-65 0.7086171628555036 1101 66-67 0.6726725111748024 1101 68-69 0.6989883719308168 1101 70-71 0.6184356864576372 1101 72-73 0.6079916124316824 1101 74-75 0.5832861634026969 1101 76-77 0.3987881799129269 1101 78-79 0.5713827863717782 1101 80-81 0.6659586926949643 1101 82-83 0.5635739817537981 1101 84-85 0.42113751635823604 1101 86-87 0.6283128751785867 1101 88-89 0.6960095895687104 1101 90-91 0.5256657362537709 1101 92-93 0.44401104002155023 1101 94-95 0.39985897862424125 1101 96-97 0.43131352714794957 1101 98-99 0.30718938260538664 1101 100-101 0.5972285524547694 1102 1 -0.06648494184375409 1102 2 -0.05439144200088464 1102 3 0.018897885679649562 1102 4 0.04721858940433776 1102 5 -0.21109526644325882 1102 6 -0.07111160864067045 1102 7 0.08611725784586355 1102 8 -0.02554542868875842 1102 9 -0.05518762061479521 1102 10-11 0.14631297007695565 1102 12-13 0.16001708041914497 1102 14-15 0.07110916919918964 1102 16-17 0.29451264326339555 1102 18-19 0.2923029493377314 1102 20-21 0.30676729834958394 1102 22-23 0.23061499588343537 1102 24-25 0.3606764315843378 1102 26-27 0.4416751928838565 1102 28-29 0.48491069815481325 1102 30-31 0.37654232720512937 1102 32-33 0.45435582883941805 1102 34-35 0.42059206322023357 1102 36-37 0.20128672711351214 1102 38-39 0.4994278246631083 1102 40-41 0.278885301344296 1102 42-43 0.34542340321507226 1102 44-45 0.32825052705943136 1102 46-47 0.5372154263407012 1102 48-49 0.5151614549751642 1102 50-51 0.4011384603293635 1102 52-53 0.39713616253438033 1102 54-55 0.4562041304854674 1102 56-57 0.5071945419683104 1102 58-59 0.5384863181149058 1102 60-61 0.5802243293755964 1102 62-63 0.48298249470786914 1102 64-65 0.5087535169566166 1102 66-67 0.37783823385153426 1102 68-69 0.6089841764200159 1102 70-71 0.5911176667387394 1102 72-73 0.4137289734553846 1102 74-75 0.38180200145598775 1102 76-77 0.4926679785284094 1102 78-79 0.4580200844628237 1102 80-81 0.2012009834438615 1102 82-83 0.5255888758171565 1102 84-85 0.3474800798153339 1102 86-87 0.5333527325312204 1102 88-89 0.4549565163570435 1102 90-91 0.28470181764667757 1102 92-93 0.2602633999963757 1102 94-95 0.11387722909623577 1102 96-97 0.04049120703047393 1102 98-99 0.10043985460035287 1102 100-101 0.0928652212191885 1103 1 -0.032517865562745385 1103 2 -0.0342198676906591 1103 3 -0.04929710620534422 1103 4 -0.032617564857964965 1103 5 -0.1056582156665371 1103 6 -0.05001260562652732 1103 7 -0.17198808259775689 1103 8 -0.09462920641501427 1103 9 0.003752405662353908 1103 10-11 -0.1311910874236233 1103 12-13 -1.382638887577059E-4 1103 14-15 -0.03526497099711179 1103 16-17 0.07021014693447825 1103 18-19 0.015494463365087086 1103 20-21 -0.047660207640063845 1103 22-23 -0.21731857703564827 1103 24-25 -0.13701948772936845 1103 26-27 0.029737446535605727 1103 28-29 -0.1240283620507654 1103 30-31 0.048271208880684924 1103 32-33 -0.07657778272253069 1103 34-35 0.2502881371053931 1103 36-37 0.20242186120424321 1103 38-39 -0.12394917510503234 1103 40-41 -0.006308685826276417 1103 42-43 -0.01925412209600097 1103 44-45 0.11282622842738732 1103 46-47 0.1611141047547946 1103 48-49 -0.0047782621590855 1103 50-51 -0.08175724258430961 1103 52-53 9.888559419124476E-4 1103 54-55 0.12042180573084948 1103 56-57 0.017154739819766007 1103 58-59 -0.14483542235961977 1103 60-61 -0.11729383276985317 1103 62-63 -0.07314626331052665 1103 64-65 0.007019418494600416 1103 66-67 -0.052609443712420045 1103 68-69 -0.004896339848635023 1103 70-71 -0.047165817301753066 1103 72-73 -0.04764748531268026 1103 74-75 0.1075342837034512 1103 76-77 0.07341224006329838 1103 78-79 0.09287401444196064 1103 80-81 -0.14215534239275485 1103 82-83 -0.15938967739406706 1103 84-85 -0.12084243351645085 1103 86-87 -0.23456306467103616 1103 88-89 -0.2858513126869333 1103 90-91 -0.38153977753069057 1103 92-93 -0.4426744236375981 1103 94-95 -0.4080670865386615 1103 96-97 -0.23842331495731628 1103 98-99 -0.4951915697755638 1103 100-101 -0.33519625957530863 1104 1 0.38226376937566187 1104 2 0.37025941562401243 1104 3 0.3874610124956632 1104 4 0.28925626866200105 1104 5 0.3647225234152103 1104 6 0.3692483126265529 1104 7 0.31044006223240217 1104 8 0.2722894199380974 1104 9 0.3448404982342126 1104 10-11 0.3019836046256543 1104 12-13 0.27438581225905523 1104 14-15 0.36461371507978413 1104 16-17 0.4450328419699403 1104 18-19 0.3416702752351952 1104 20-21 0.4472959327108157 1104 22-23 0.4400582426731958 1104 24-25 0.5891983174218112 1104 26-27 0.3730605148446813 1104 28-29 0.41808156702725086 1104 30-31 0.30732309391240875 1104 32-33 0.3451094139926667 1104 34-35 0.4029990366947871 1104 36-37 0.45158666150285853 1104 38-39 0.45670592007973454 1104 40-41 0.3259328221990998 1104 42-43 0.522351887609112 1104 44-45 0.42959554533667443 1104 46-47 0.4063268707122418 1104 48-49 0.3114591398565878 1104 50-51 0.22432245133133222 1104 52-53 0.21703962115280717 1104 54-55 0.26591919281558063 1104 56-57 0.5047153967813145 1104 58-59 0.32410821332533146 1104 60-61 0.37587059425515434 1104 62-63 0.44732779417361 1104 64-65 0.4293365144259198 1104 66-67 0.3357677492700404 1104 68-69 0.4027371054667839 1104 70-71 0.45610032678185775 1104 72-73 0.41883254454920404 1104 74-75 0.5008900134533576 1104 76-77 0.3587425872077574 1104 78-79 0.4100352686412876 1104 80-81 0.3212778416311366 1104 82-83 -0.019734022298884213 1104 84-85 0.10824491250817658 1104 86-87 0.10599777892732476 1104 88-89 0.014601281564658564 1104 90-91 -2.9677641087033635E-4 1104 92-93 -0.28615613323072964 1104 94-95 -0.21815013991679066 1104 96-97 0.03734189594227644 1104 98-99 0.11881552242301652 1104 100-101 0.2884646213504105 1105 1 0.21882477675733725 1105 2 0.2141152558324393 1105 3 0.13778667467542505 1105 4 0.33233051963898674 1105 5 0.1346747596288438 1105 6 0.2537467928697126 1105 7 0.2074873918025304 1105 8 0.15804712727635462 1105 9 0.2831383705746333 1105 10-11 0.2723266354550091 1105 12-13 0.24153735372671292 1105 14-15 0.2524990819925108 1105 16-17 0.545380214961682 1105 18-19 0.4840497802286805 1105 20-21 0.3870918510781607 1105 22-23 0.40686241114909905 1105 24-25 0.5115170321417466 1105 26-27 0.5963996377278704 1105 28-29 0.5549682365886923 1105 30-31 0.45215592066013244 1105 32-33 0.583125045777301 1105 34-35 0.5300724192392963 1105 36-37 0.5794850549027757 1105 38-39 0.66028820405716 1105 40-41 0.4760847978829972 1105 42-43 0.6285394690246591 1105 44-45 0.5523267654843167 1105 46-47 0.4646855333262252 1105 48-49 0.5882937034692688 1105 50-51 0.6049998286652496 1105 52-53 0.6867747492465952 1105 54-55 0.6172218415346435 1105 56-57 0.7760788357739372 1105 58-59 0.5329662246149525 1105 60-61 0.542001727559537 1105 62-63 0.3509317889630168 1105 64-65 0.3184811084337369 1105 66-67 0.3041350962088174 1105 68-69 0.0656550385974839 1105 70-71 0.1584016728522002 1105 72-73 0.2229575988262411 1105 74-75 0.27733378245032014 1105 76-77 0.2544004248108891 1105 78-79 0.10675693603165115 1105 80-81 0.2424893049398591 1105 82-83 0.010682825291219444 1105 84-85 0.40668173404530705 1105 86-87 0.3128366847023969 1105 88-89 0.2681184331061175 1105 90-91 0.03161811720615759 1105 92-93 0.2526845094092991 1105 94-95 0.253430407195669 1105 96-97 0.29695978565135306 1105 98-99 0.18218938260538664 1105 100-101 0.32239862048197665 1106 1 0.16090583453256357 1106 2 0.07189047859760223 1106 3 0.08814597332446539 1106 4 0.18562169526416739 1106 5 0.15752615370760026 1106 6 0.2756783824069373 1106 7 0.2421237815697026 1106 8 0.22536532215994498 1106 9 0.2664381693673903 1106 10-11 0.08665546557860893 1106 12-13 0.21552413153642647 1106 14-15 0.24738266923021968 1106 16-17 0.44076682030804903 1106 18-19 0.2961653306742065 1106 20-21 0.2558472405578982 1106 22-23 0.39954709065470695 1106 24-25 0.3704132666919051 1106 26-27 0.3111165103924307 1106 28-29 0.42497398536707465 1106 30-31 0.49048590053940444 1106 32-33 0.30139178909434605 1106 34-35 0.3473331263390378 1106 36-37 0.27800474446874546 1106 38-39 0.35790246102754963 1106 40-41 0.49912302725925173 1106 42-43 0.2527705699157181 1106 44-45 0.4056754288933391 1106 46-47 0.10301551320549862 1106 48-49 0.21940896647588204 1106 50-51 0.11329244148502227 1106 52-53 0.26166695965189035 1106 54-55 0.1765061186257597 1106 56-57 0.1548802154807447 1106 58-59 0.20290011366353156 1106 60-61 0.38511756544398423 1106 62-63 0.30282888582993195 1106 64-65 0.19235291067575844 1106 66-67 0.3856671456483127 1106 68-69 0.46515202048883 1106 70-71 0.18119557914710782 1106 72-73 0.3531961731292057 1106 74-75 0.3226054122289881 1106 76-77 0.42197731472177935 1106 78-79 0.5452593792624683 1106 80-81 0.543222274183897 1106 82-83 0.5667765303788812 1106 84-85 0.5286995552582923 1106 86-87 0.5023739080424363 1106 88-89 0.309825820286342 1106 90-91 0.5365476946709364 1106 92-93 0.542753494749924 1106 94-95 0.6045370470347073 1106 96-97 0.7905355258065612 1106 98-99 0.640539483209011 1106 100-101 0.6336805980617406 1107 1 -0.08353001131173698 1107 2 0.023573654576551917 1107 3 0.15313204359222254 1107 4 0.22726772529048134 1107 5 0.34586000295693964 1107 6 0.25292261704554164 1107 7 0.15156117516202983 1107 8 0.12143017280225621 1107 9 0.08890760134386255 1107 10-11 0.1013493984063345 1107 12-13 0.21190627052419586 1107 14-15 0.06492058905686804 1107 16-17 0.1314280956524172 1107 18-19 0.06266045526792396 1107 20-21 0.1819113173261968 1107 22-23 0.18156806264047276 1107 24-25 0.25042990498318574 1107 26-27 0.1131382562522063 1107 28-29 0.2381511251287236 1107 30-31 0.26534273384694274 1107 32-33 0.32072316194683737 1107 34-35 0.2749507551890602 1107 36-37 0.2312511459545874 1107 38-39 0.27812572368038246 1107 40-41 0.14984853414673438 1107 42-43 0.2681784015207356 1107 44-45 0.4557412081844703 1107 46-47 0.18803718167787054 1107 48-49 0.21698287144415218 1107 50-51 0.2624566575506435 1107 52-53 0.2390117979122195 1107 54-55 0.6353340864326 1107 56-57 0.4319658059466178 1107 58-59 0.16709102838261458 1107 60-61 0.24319874482798554 1107 62-63 0.3644718203601869 1107 64-65 0.17780214454048604 1107 66-67 0.3021139031161937 1107 68-69 0.4788418517843027 1107 70-71 0.46344484396679775 1107 72-73 0.5468194512595232 1107 74-75 0.522800815417348 1107 76-77 0.5542826854074292 1107 78-79 0.526831504320505 1107 80-81 0.49672792346419925 1107 82-83 0.6966325898124097 1107 84-85 0.6651393478600625 1107 86-87 0.7850894319551429 1107 88-89 0.6911561097152301 1107 90-91 0.8412335018215416 1107 92-93 0.6673626883731956 1107 94-95 0.7980732643385302 1107 96-97 0.7152878861223115 1107 98-99 0.6157058661218713 1107 100-101 0.5804245231507394 1108 1 0.055786228004507166 1108 2 -0.01638361038071423 1108 3 0.05192121738139832 1108 4 0.15013097315372903 1108 5 0.06373749583443811 1108 6 0.11168330080622013 1108 7 0.05476630336715971 1108 8 0.17705695342903738 1108 9 0.31647170390796475 1108 10-11 0.1818693414262853 1108 12-13 0.13256154117946295 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40-41 -1.0241108968332497 2316 42-43 -0.6866688060662014 2316 44-45 -0.9795854514572611 2316 46-47 -0.9748447993547913 2316 48-49 -1.2262995310848268 2316 50-51 -1.1209996122069938 2316 52-53 -1.014860699453429 2316 54-55 -1.0343576943893211 2316 56-57 -1.0061269232420003 2316 58-59 -1.0944869484350903 2316 60-61 -1.1758344480065759 2316 62-63 -1.2041003608832241 2316 64-65 -1.0051140830675251 2316 66-67 -1.0368349904559864 2316 68-69 -1.072896820502315 2316 70-71 -0.8532701191522776 2316 72-73 -0.8417474612799936 2316 74-75 -1.5115116187238513 2316 76-77 -1.3325718983649608 2316 78-79 -0.9829551131715917 2316 80-81 -0.7443152799077737 2316 82-83 -0.9111650919578764 2316 84-85 -0.7930666584623829 2316 86-87 -0.8199421802682494 2316 88-89 -0.6718312453954169 2316 90-91 -0.7101627047116565 2316 92-93 -0.9705192926594677 2316 94-95 -0.9675089274422923 2316 96-97 -0.7677732308429199 2316 98-99 -0.9329769579034206 2316 100-101 -0.5702208930768684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 17.0 7 666.0 8 1840.0 9 1318.0 10 1154.0 11 1312.0 12 1448.0 13 1415.0 14 1236.0 15 1117.0 16 1131.0 17 1423.0 18 1621.0 19 1947.0 20 2172.0 21 2388.0 22 2529.0 23 2619.0 24 2653.0 25 2688.0 26 3026.0 27 3511.0 28 4071.0 29 5018.0 30 6035.0 31 7402.0 32 9467.0 33 12975.0 34 19981.0 35 33191.0 36 68072.0 37 159507.0 38 155390.0 39 13844.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.220445908759054 15.803553042924804 11.901196203597971 26.07480484471817 2 17.74069151425523 19.43540687770269 35.204702446694995 27.619199161347087 3 20.102023624553063 22.146801699769743 28.087384638424528 29.663790037252664 4 14.233512420674291 16.054212920496454 33.96300941612535 35.749265242703906 5 16.280911286246464 35.277710178026545 32.38342162900841 16.057956906718584 6 35.83313053407963 33.128287687901306 16.223066699114547 14.81551507890451 7 30.590801025852226 30.300080495703774 19.353600778749133 19.755517699694867 8 28.911810404537714 30.971190026020707 18.99342930418016 21.123570265261424 9 28.15271719800071 13.891312079971547 17.521481120949474 40.43448960107827 10-11 26.76257511372358 23.449053707482356 27.507160373649825 22.281210805144237 12-13 27.816694434564482 21.656339504670623 25.88320634980063 24.643759710964265 14-15 25.329002789269733 22.86967183960763 23.241262472154105 28.560062898968532 16-17 25.512083715531926 24.916415507590933 24.05679627098972 25.51470450588742 18-19 25.356146689380182 24.8281510324042 25.12720193189689 24.688500346318726 20-21 26.329021509200846 24.375690297459705 24.55306164473315 24.7422265486063 22-23 26.145965055471887 24.21125836430705 24.285857359607405 25.356919220613662 24-25 25.573345763390858 24.766421622309707 24.272214515836154 25.388018098463277 26-27 25.837716917201746 24.41846534004755 24.219285273030195 25.524532469720512 28-29 25.913626237855446 24.403021396881257 24.374473501937512 25.308878863325784 30-31 26.211460341825944 24.493064265523504 24.07364420898931 25.22183118366124 32-33 25.712386978415918 24.78144480428312 24.17903742114229 25.32713079615867 34-35 26.21080514423707 24.46769875886857 24.255882738351524 25.065613358542844 36-37 26.176360470993465 24.30736254890582 24.282839439150862 25.23343754094985 38-39 25.945824519365768 24.401804601359068 24.160504689342744 25.491866189932423 40-41 26.26294015238024 24.128025608865762 24.42623411145847 25.18280012729553 42-43 25.897807896066944 24.18446620116438 24.49671465209008 25.4210112506786 44-45 25.980924390198247 24.251296355229414 24.302214567850392 25.465564686721955 46-47 25.88180235496733 24.113330462943896 24.11258166569947 25.8922855163893 48-49 25.804769838446994 23.910312810048858 24.327860873471984 25.957056478032158 50-51 25.558228345719687 24.29182500608398 24.284337033639716 25.865609614556618 52-53 25.7218405436268 24.347329601827063 23.872311349894233 26.0585185046519 54-55 25.947228514199068 24.186899792208767 23.963758213369772 25.902113480222393 56-57 25.48147662816601 24.388139051648288 24.05876186375634 26.07162245642936 58-59 25.82667215784646 24.455343604335535 24.162844680731574 25.55513955708643 60-61 26.124506261816954 24.156854302776164 24.072708212433778 25.6459312229731 62-63 25.908665456111123 24.302588966472605 24.18596379565323 25.602781781763046 64-65 25.834815327879596 24.282184241561993 24.014208427712987 25.86879200284543 66-67 25.49645257305453 24.472191542335125 24.20814691401936 25.82320897059099 68-69 25.303543682959244 24.13981916546547 24.269080289784533 26.28755686179075 70-71 25.504314944121003 23.79855482131826 24.289765813661806 26.407364420898933 72-73 25.311780452647938 24.229394035829948 24.100132911510887 26.35869260001123 74-75 25.48727980681031 24.1521743199985 24.21254609783036 26.147999775360827 76-77 25.47005747018851 24.14758793687639 24.212826896797022 26.16952769613808 78-79 25.200958460472865 24.400400606525768 23.870345757127616 26.52829517587375 80-81 25.554016361219787 23.918830378704207 24.219566071996855 26.307587188079147 82-83 25.571706696119357 24.131675995432335 24.487822684812517 25.808794623635784 84-85 25.61569853422939 24.224152455118965 24.020479604635057 26.139669406016587 86-87 25.342293940358303 24.009622044590877 24.344708811471573 26.30337520357925 88-89 25.497201370298956 24.255040341451544 24.237256406896424 26.010501881353076 90-91 25.422134446545236 24.182687807708867 24.078136992455867 26.317040753290026 92-93 25.987008367809207 23.744735019375128 24.607349444954043 25.660907167861623 94-95 25.320110821992177 23.921357569404144 24.644414908553138 26.114116700050545 96-97 25.235871131994237 24.03002676950149 24.49961624141223 26.234485857092043 98-99 25.398266534379154 23.833561092495177 24.507104213856493 26.261068159269175 100-101 25.660626368894963 23.70841835302046 24.400962204459088 26.22999307362549 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 44.0 1 30.5 2 20.5 3 29.0 4 26.0 5 31.5 6 50.0 7 92.0 8 151.0 9 163.0 10 141.0 11 126.5 12 142.5 13 153.5 14 173.0 15 191.5 16 197.0 17 216.0 18 227.5 19 246.0 20 271.0 21 327.0 22 391.0 23 417.5 24 474.5 25 570.5 26 654.0 27 733.0 28 848.5 29 965.0 30 1062.5 31 1204.0 32 1395.0 33 1508.5 34 1570.5 35 1735.0 36 1955.0 37 2084.0 38 2229.0 39 2513.5 40 3062.0 41 3963.5 42 5372.5 43 7583.0 44 10814.0 45 14885.5 46 19082.5 47 23296.5 48 27429.0 49 30696.0 50 32336.5 51 32529.0 52 32566.5 53 32651.0 54 31574.5 55 29734.5 56 27510.0 57 26539.0 58 26928.5 59 23363.5 60 17032.0 61 12459.5 62 9305.5 63 6863.5 64 5134.5 65 3845.5 66 2897.0 67 2350.0 68 1875.5 69 1404.0 70 1002.5 71 720.0 72 497.5 73 347.5 74 285.0 75 234.0 76 159.0 77 110.5 78 86.0 79 64.5 80 52.5 81 44.5 82 32.5 83 26.0 84 23.5 85 21.0 86 17.0 87 13.5 88 7.5 89 4.5 90 5.5 91 3.0 92 0.5 93 1.0 94 1.0 95 1.5 96 1.0 97 0.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.359965555326756E-5 24-25 1.8719931110653513E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 534190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.11920015828909 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5119339242923 46.907409377826134 2 14.58972481267676 18.12604071786049 3 4.8009082512093775 8.946857417953707 4 2.077498390527267 5.162101533987469 5 0.9825269190317195 3.0516893172119244 6 0.6064790649072548 2.2604396654871457 7 0.36065233463682905 1.5682404194001611 8 0.2412625514537709 1.1989629379565052 9 0.15913363185390367 0.8896728536143317 >10 0.6095433588715541 6.187731664497674 >50 0.03047311318937759 1.3137179675294703 >100 0.027730529682060117 3.5612354138391424 >500 0.002133117667850715 0.8259007128358018 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC 786 0.1471386585297366 No Hit CCATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT 764 0.14302027368539283 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.13309871019674646 No Hit ATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGC 587 0.10988599561953612 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.8719931110653513E-4 0.0 0.0 0.0 0.0 6 1.8719931110653513E-4 0.0 0.0 0.0 0.0 7 1.8719931110653513E-4 0.0 0.0 0.0 0.0 8 1.8719931110653513E-4 0.0 0.0 0.0 0.0 9 1.8719931110653513E-4 0.0 0.0 0.0 0.0 10-11 3.7439862221307025E-4 0.0 0.0 0.0 0.0 12-13 3.7439862221307025E-4 0.0 0.0 0.0 0.0 14-15 3.7439862221307025E-4 0.0 0.0 0.0 0.0 16-17 3.7439862221307025E-4 0.0 0.0 0.0 0.0 18-19 3.7439862221307025E-4 0.0 0.0 1.8719931110653513E-4 0.0 20-21 3.7439862221307025E-4 0.0 0.0 7.487972444261405E-4 0.0 22-23 3.7439862221307025E-4 0.0 0.0 0.00383758587768397 0.0 24-25 4.679982777663378E-4 9.359965555326756E-5 0.0 0.010108762799752896 0.0 26-27 5.615979333196054E-4 1.8719931110653513E-4 0.0 0.01740953593290777 0.0 28-29 5.615979333196054E-4 1.8719931110653513E-4 0.0 0.03921825567681911 0.0 30-31 5.615979333196054E-4 1.8719931110653513E-4 0.0 0.07338212995376177 0.0 32-33 5.615979333196054E-4 1.8719931110653513E-4 0.0 0.11325558321945375 0.0 34-35 5.615979333196054E-4 1.8719931110653513E-4 0.0 0.17222336621801232 0.0 36-37 5.615979333196054E-4 1.8719931110653513E-4 0.0 0.2530934686160355 0.0 38-39 5.615979333196054E-4 1.8719931110653513E-4 0.0 0.37327542634643107 0.0 40-41 7.487972444261405E-4 1.8719931110653513E-4 0.0 0.5059997379209644 0.0 42-43 7.487972444261405E-4 1.8719931110653513E-4 0.0 0.6157921338849472 0.0 44-45 7.487972444261405E-4 1.8719931110653513E-4 0.0 0.7308261105599132 0.0 46-47 7.487972444261405E-4 1.8719931110653513E-4 0.0 0.8592448379789962 0.0 48-49 7.487972444261405E-4 1.8719931110653513E-4 0.0 0.9952451374978939 0.0 50-51 7.487972444261405E-4 1.8719931110653513E-4 0.0 1.1446301877609089 0.0 52-53 7.487972444261405E-4 1.8719931110653513E-4 0.0 1.285216870401917 0.0 54-55 7.487972444261405E-4 1.8719931110653513E-4 0.0 1.4368483123982103 0.0 56-57 7.487972444261405E-4 1.8719931110653513E-4 0.0 1.620303637282615 0.0 58-59 7.487972444261405E-4 1.8719931110653513E-4 0.0 1.8168629139444765 0.0 60-61 7.487972444261405E-4 1.8719931110653513E-4 0.0 2.0365413055279955 0.0 62-63 7.487972444261405E-4 1.8719931110653513E-4 0.0 2.2523821112338305 0.0 64-65 7.487972444261405E-4 1.8719931110653513E-4 0.0 2.4849772552837006 0.0 66-67 7.487972444261405E-4 1.8719931110653513E-4 0.0 2.7599730432992007 0.0 68-69 9.359965555326757E-4 1.8719931110653513E-4 0.0 3.065856717647279 0.0 70-71 9.359965555326757E-4 1.8719931110653513E-4 0.0 3.3814747561728975 0.0 72-73 9.359965555326757E-4 1.8719931110653513E-4 0.0 3.696437597109643 0.0 74-75 9.359965555326757E-4 1.8719931110653513E-4 0.0 4.038263539190176 0.0 76-77 9.359965555326757E-4 1.8719931110653513E-4 0.0 4.418090941425335 0.0 78-79 9.359965555326757E-4 1.8719931110653513E-4 0.0 4.843501375914936 0.0 80-81 9.359965555326757E-4 1.8719931110653513E-4 0.0 5.310376457814636 0.0 82-83 9.359965555326757E-4 1.8719931110653513E-4 0.0 5.794099477713922 0.0 84-85 9.359965555326757E-4 1.8719931110653513E-4 0.0 6.286059267301896 0.0 86-87 9.359965555326757E-4 1.8719931110653513E-4 0.0 6.843445216121605 0.0 88-89 9.359965555326757E-4 1.8719931110653513E-4 0.0 7.482356464928209 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 745 0.0 38.25503 1 GTATCAA 1065 0.0 36.126762 1 GTATAAG 100 1.297467E-6 33.250004 1 GTATAAT 60 0.003949075 31.666668 1 TTTATAT 110 2.7298393E-6 30.227272 3 TATCAAC 1260 0.0 29.781746 2 TCAACGC 1285 0.0 28.463036 4 ATCAACG 1325 0.0 27.962263 3 CAACGCA 1310 0.0 27.919847 5 AACGCAG 1310 0.0 27.919847 6 GTACATG 1430 0.0 27.9021 1 GTTCATA 120 5.3746044E-6 27.708334 1 TACATGG 1410 0.0 25.939716 2 ATAATGC 130 1.0007978E-5 25.576923 3 ACGCAGA 1510 0.0 24.221853 7 TATAAGG 120 1.7091463E-4 23.75 2 CGCAGAG 1560 0.0 23.445513 8 GTATAAA 125 2.2537084E-4 22.8 1 TATAAGA 105 0.0021946935 22.619047 2 TATACAG 130 2.9386563E-4 21.923077 5 >>END_MODULE