FastQCFastQC Report
Fri 10 Feb 2017
SRR2031852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534190
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT54981.02922181246373No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT18950.35474269454688406RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT16270.3045732791703326RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.1639865965293248No Hit
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC8650.1619274041071529No Hit
CCATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT7620.14264587506317977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.12692113293023083No Hit
GTGCAGGGTGGACTCTTTCTGGATGTTGTAGTCTGACAGGGTGCGGCCAT5770.10801400250847076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.041.348631
GTATTAC608.1992344E-539.653121
GTATCAA9800.034.959481
TAGCGCA702.0520917E-433.9279379
TCAACGC11850.029.6618944
ATCTTAT856.3370366E-427.9904351
CAACGCA12750.027.5681135
ATCAACG12750.027.5681133
TATCAAC12600.027.5193272
AACGCAG13150.026.7295386
TATATAC908.9422276E-426.3883975
TACTGAC1806.682967E-926.3883977
CGCAGAG14650.024.316958
ACGCAGA14600.024.0748927
TTATATA1401.777426E-523.7495564
TATTAAG1000.001652670923.7495562
GTACATG11650.023.2813151
TAATACT1652.4262317E-623.0298734
GGCTAGT1050.002194932422.6186247
CATGGGG9050.022.5686384
Produced by FastQC (version 0.11.5)