FastQCFastQC Report
Fri 10 Feb 2017
SRR2031851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.6007067137809188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.49469964664310956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.3886925795053004No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.21201413427561835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.17667844522968199No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1413427561837456No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT40.1413427561837456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1413427561837456No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30.10600706713780918No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30.10600706713780918No Hit
GGTATCAACGCAGAGTACATAAGCAGTGGTATCACTGTCTCTTATACACA30.10600706713780918No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10600706713780918No Hit
TGATACCACTGCTTATGTACTCTGCGTTGATACCTGTCTCTTATACACAT30.10600706713780918No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA155.944046E-495.09
ACGCAGA301.0885489E-463.3333368
TATCAAC301.0885489E-463.3333363
ATCAACG301.0885489E-463.3333364
TCAACGC352.3340713E-454.285715
GGTATCA352.3340713E-454.285711
AACGCAG404.5144634E-447.4999967
GTATCAA404.5144634E-447.4999962
CAACGCA404.5144634E-447.4999966
TCTCGGT250.001482342138.088-89
TCGGTGG300.00362734231.66666890-91
GATCTCG300.00362734231.66666886-87
TGTAGAT300.00362734231.66666882-83
GGTGGTC300.00362734231.66666892-93
TAGATCT300.00362734231.66666884-85
ACGCTGC350.00771007927.14285560-61
TGCCGAC350.00771007927.14285564-65
CATCTGA350.00771007927.14285554-55
CTCTTAT350.00771007927.14285544-45
TACACAT350.00771007927.14285550-51
Produced by FastQC (version 0.11.5)