FastQCFastQC Report
Fri 10 Feb 2017
SRR2031851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT57920.459363957597173No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT41014.487632508833922RNA PCR Primer, Index 46 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT34212.084805653710246RNA PCR Primer, Index 46 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT1123.9575971731448765RNA PCR Primer, Index 46 (96% over 26bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG993.498233215547703RNA PCR Primer, Index 46 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG742.614840989399293TruSeq Adapter, Index 4 (95% over 23bp)
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC431.519434628975265TruSeq Adapter, Index 18 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC220.7773851590106008No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA190.6713780918727915RNA PCR Primer, Index 46 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT180.6360424028268552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.6007067137809188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.5300353356890459No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC140.49469964664310956No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTT120.4240282685512367No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT100.35335689045936397No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90.3180212014134276No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAAGGCG80.2826855123674912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.24734982332155478No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTTCT70.24734982332155478Illumina PCR Primer Index 8 (95% over 21bp)
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT70.24734982332155478TruSeq Adapter, Index 4 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.21201413427561835No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGC60.21201413427561835No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.17667844522968199No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.17667844522968199No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGC40.1413427561837456No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG40.1413427561837456No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1413427561837456No Hit
NTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT40.1413427561837456RNA PCR Primer, Index 46 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC40.1413427561837456No Hit
GGCTCGGAGATGTGTATAAGAGACAGACTCCGAGCCCACGAGACTAAGGC40.1413427561837456No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30.10600706713780918No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCT30.10600706713780918Illumina PCR Primer Index 8 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTT30.10600706713780918No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGT30.10600706713780918No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTTCTGCT30.10600706713780918Illumina PCR Primer Index 8 (95% over 24bp)
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10600706713780918No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGTATGCCGT30.10600706713780918No Hit
TGATACCACTGCTTATGTACTCTGCGTTGATACCCTGTCTCTTATACACA30.10600706713780918No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT30.10600706713780918No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG30.10600706713780918TruSeq Adapter, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10600706713780918No Hit
GTATCAACGCAGAGTACATAAGCAGTGGTATCACTGTCTCTTATACACAT30.10600706713780918No Hit
TCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA30.10600706713780918TruSeq Adapter, Index 4 (96% over 27bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA30.10600706713780918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1000.061.751
TATACAC1050.058.8095253
TTATACA1050.058.8095252
TACACAT1500.041.1666685
ATACACA1500.041.1666684
ACACATC1950.031.6666686
CACATCT2050.030.1219527
ACATCTC2100.029.4047628
CATCTCC2200.028.0681849
CTCTTAT350.00771007927.14285544-45
GTCTCTT350.00771007927.14285542-43
CTGTCTC350.00771007927.14285540-41
CCGAGCC2353.0900992E-820.2127659
TCTCCGA2359.640644E-1120.2127656
TCCGAGC2359.640644E-1120.2127658
CTCCGAG2353.0900992E-820.2127657
ATCTCCG2201.2925739E-819.431825
TGCTTGA2009.094947E-1217.812554-55
CTTGAAA2009.094947E-1217.812556-57
TCTGCTT2051.2732926E-1117.3780552-53
Produced by FastQC (version 0.11.5)