FastQCFastQC Report
Fri 10 Feb 2017
SRR2031849_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031849_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50240
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6011.1962579617834397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.9156050955414012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.6468949044585988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.6090764331210191No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.4538216560509554No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.302547770700637No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.30055732484076436No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.17515923566878983No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA820.16321656050955416No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.15923566878980894No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA720.14331210191082802No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.12738853503184713No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG640.12738853503184713No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA590.11743630573248408No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT560.11146496815286625No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.11146496815286625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA200.00206250671.251
CCAGTGT250.00499342257.08
GGTATCA6600.035.9848481
TCATTAA658.129064E-932.88461794-95
CATTAAA601.4826946E-731.66666894-95
CGCAGAA1051.8542305E-631.6666669
GTATCAA9550.031.3350771
ATGGGGA650.00579168329.230777
AACGCAG10100.029.1584196
TATCATT753.2730895E-828.49999892-93
CAACGCA10500.028.047625
TATCAAC10700.027.5233672
TCAACGC10700.027.5233674
ACGCAGA10750.027.395357
GTCGGGG350.008355780527.14285932-33
ATCAACG11100.026.959463
CGCAGAG10150.026.6748758
AGAGTAA659.902777E-625.57692310-11
GCAGAGT10100.025.3960429
ATCATTA801.8315568E-623.74999892-93
Produced by FastQC (version 0.11.5)