FastQCFastQC Report
Fri 10 Feb 2017
SRR2031849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50240
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT45589.072452229299364No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT18043.590764331210191RNA PCR Primer, Index 46 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT17813.544984076433121RNA PCR Primer, Index 46 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT7091.4112261146496816RNA PCR Primer, Index 46 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6801.3535031847133758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5851.1644108280254777No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG4690.9335191082802548RNA PCR Primer, Index 46 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.8160828025477707No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG3980.7921974522292994TruSeq Adapter, Index 4 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.605095541401274No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.5453821656050956No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC2290.455812101910828TruSeq Adapter, Index 18 (95% over 24bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.3463375796178344No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.2627388535031847No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.24084394904458598No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC1150.2289012738853503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.2289012738853503No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT1000.19904458598726116No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC980.19506369426751594No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA790.1572452229299363RNA PCR Primer, Index 46 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.1512738853503185No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA750.14928343949044587No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGC710.1413216560509554No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAAGGCG690.13734076433121017No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA660.13136942675159236No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT620.12340764331210191No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT600.11942675159235669TruSeq Adapter, Index 4 (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT590.11743630573248408No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.10748407643312102No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT520.1035031847133758No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT510.10151273885350319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA254.5561683E-795.01
ACCCATG254.5561683E-795.03
TACCCAT353.369656E-667.857152
TGTGTAC250.00499342257.09
GGTATCA5600.044.955361
TATACAC13600.039.1176453
TTATACA13750.038.690912
CTTATAC13650.038.6263731
CGCAGAA1451.6370905E-1136.034489
ATGGGGA550.002556292834.5454565
ATACACA15550.034.212224
TACACAT15500.034.016135
GTATCAA8500.032.970591
CACATCT17950.029.3732597
AACGCAG9600.029.1927096
ACACATC18100.029.1298356
ACATCTC18300.028.8114768
TATCAAC9800.028.5969412
TCAACGC9800.028.5969414
CAACGCA9900.028.3080835
Produced by FastQC (version 0.11.5)