FastQCFastQC Report
Fri 10 Feb 2017
SRR2031847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49081
Sequences flagged as poor quality0
Sequence length101
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8541.7399808479859824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8381.707381675189992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7811.5912471221042765No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5741.169495324056152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.9351887695849718No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.7008822151137916No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.5501110409323363No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.39526497015138246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.31376703816140666No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.23634400277092968No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.2322691061714309No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.21393207147368637No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.20781972657443817No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT820.16707076057945028No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.1385464843829588No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG670.13650903608320938No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA620.12632179458446244No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT610.12428434628471302No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.11613455308571545No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT560.11409710478596606No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.11205965648621666No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCAT200.002062222771.259
CGCAGAA1800.063.3333329
TATATGG250.004992739857.04
GGAGTTC250.004992739857.06
AGTTCCA250.004992739857.08
ACAGAGC250.004992739857.08
GGTATCA8250.042.0303041
CAGGAGT500.001600776838.04
GTTCAGG500.001600776838.01
CTCCTGA500.001600776838.01
GCCGTAT401.0676036E-535.62594-95
CAGAAAA2150.033.13953410-11
GTATCAA10550.032.86732
AACGCAG11250.031.2444447
TATCAAC11500.030.1521743
CAACGCA11800.029.7881366
TCAACGC11850.029.6624475
ACGCAGA12000.029.2916688
ATCAACG12150.028.5390954
TCGCCGT504.9076996E-528.592-93
Produced by FastQC (version 0.11.5)