##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031847_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49081 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15236038385526 33.0 31.0 34.0 28.0 34.0 2 31.252949206413888 34.0 31.0 34.0 28.0 34.0 3 31.20472280515882 34.0 31.0 34.0 27.0 34.0 4 34.4007864550437 37.0 35.0 37.0 30.0 37.0 5 34.45001120596565 37.0 35.0 37.0 30.0 37.0 6 34.62955115013956 37.0 35.0 37.0 31.0 37.0 7 34.65650659114525 37.0 35.0 37.0 31.0 37.0 8 34.64199995925103 37.0 35.0 37.0 31.0 37.0 9 36.29787494142336 39.0 37.0 39.0 32.0 39.0 10-11 36.34930013650904 39.0 37.5 39.0 31.5 39.0 12-13 36.324718322772554 39.0 38.0 39.0 31.0 39.0 14-15 37.613027444428596 41.0 38.0 41.0 31.5 41.0 16-17 37.53474868075223 41.0 38.0 41.0 31.5 41.0 18-19 37.54602595709134 41.0 38.0 41.0 31.5 41.0 20-21 37.51075772702268 41.0 38.0 41.0 31.5 41.0 22-23 37.34599947026344 41.0 38.0 41.0 31.0 41.0 24-25 37.25668792404393 40.5 37.5 41.0 31.0 41.0 26-27 36.985758236384754 40.0 36.5 41.0 30.5 41.0 28-29 36.678195228296076 40.0 35.0 41.0 30.0 41.0 30-31 36.28968439925837 39.5 35.0 41.0 29.0 41.0 32-33 35.87019416882296 39.0 35.0 41.0 27.5 41.0 34-35 35.53018479656079 39.0 35.0 41.0 25.0 41.0 36-37 35.28200321916832 38.5 35.0 41.0 24.0 41.0 38-39 34.94771907662843 38.0 35.0 41.0 21.5 41.0 40-41 34.6186915507019 38.0 35.0 41.0 18.5 41.0 42-43 34.15144353212037 38.0 35.0 41.0 15.0 41.0 44-45 33.802367514924306 38.0 34.5 41.0 11.0 41.0 46-47 33.193262158472734 38.0 33.0 41.0 9.0 41.0 48-49 32.61470833927589 38.0 33.0 41.0 8.0 41.0 50-51 31.462704508873088 36.5 30.0 40.0 7.0 40.5 52-53 31.39927874330189 37.0 30.0 39.5 7.0 40.5 54-55 31.853833458975977 38.0 30.0 41.0 7.0 41.0 56-57 31.714258063201648 37.0 28.5 41.0 7.0 41.0 58-59 31.528972514822435 37.0 28.0 41.0 7.0 41.0 60-61 31.25567938713555 36.5 27.5 41.0 7.0 41.0 62-63 30.87667325441617 36.0 26.0 40.0 7.0 41.0 64-65 30.511929259795032 35.0 25.0 40.0 7.0 41.0 66-67 30.159389580489396 35.0 24.0 39.0 7.0 41.0 68-69 29.740785640064384 35.0 22.5 39.0 7.0 41.0 70-71 29.30391597563212 35.0 21.0 37.5 7.0 40.5 72-73 28.849014893747068 35.0 20.0 37.0 7.0 39.5 74-75 28.465587498217232 35.0 19.5 36.5 7.0 39.0 76-77 28.119149976569346 35.0 18.5 36.0 7.0 39.0 78-79 27.77567694219759 35.0 16.5 36.0 7.0 37.5 80-81 27.48567673845276 35.0 15.0 35.0 7.0 37.0 82-83 27.21831258531815 35.0 15.0 35.0 7.0 37.0 84-85 26.923626250483892 35.0 12.0 35.0 7.0 36.0 86-87 26.68608015321611 35.0 11.0 35.0 7.0 36.0 88-89 26.479482895621523 34.0 10.0 35.0 7.0 36.0 90-91 26.275065707707668 34.0 10.0 35.0 7.0 35.0 92-93 26.107363338155295 34.0 10.0 35.0 7.0 35.0 94-95 25.915466270043396 34.0 10.0 35.0 7.0 35.0 96-97 25.778498808092742 34.0 10.0 35.0 7.0 35.0 98-99 25.609757339907503 34.0 10.0 35.0 7.0 35.0 100-101 24.39774046983558 31.5 9.0 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6941210695582676 1101 2 0.6382665701652961 1101 3 0.4228708743686589 1101 4 1.0300501369525037 1101 5 0.7013264759030804 1101 6 -0.4768865149327084 1101 7 0.07482557366777343 1101 8 0.46520086949215766 1101 9 -0.009369941893140776 1101 10-11 0.22454937274635967 1101 12-13 0.37458825364142 1101 14-15 0.2221464745068431 1101 16-17 0.9979126447972178 1101 18-19 0.3714306254392028 1101 20-21 0.560072042006162 1101 22-23 -0.2021207625737489 1101 24-25 -0.05995291254589574 1101 26-27 0.995779418820824 1101 28-29 0.590181497129727 1101 30-31 0.4230685539838248 1101 32-33 1.2986320318673918 1101 34-35 0.6496786422233285 1101 36-37 0.6772223748251776 1101 38-39 1.1528590611755547 1101 40-41 1.2601470840627513 1101 42-43 1.8776686693058835 1101 44-45 1.4350380311808308 1101 46-47 1.2790322214792553 1101 48-49 2.585757139248507 1101 50-51 2.5135594389189606 1101 52-53 1.742271721523597 1101 54-55 1.198473519638192 1101 56-57 1.4155856087261682 1101 58-59 1.8249210895633325 1101 60-61 1.3050843992550547 1101 62-63 1.4181797623912509 1101 64-65 1.5815979539036569 1101 66-67 1.3375617098746488 1101 68-69 1.343851995553237 1101 70-71 1.132304970522835 1101 72-73 1.5378101411675544 1101 74-75 1.0297408220383666 1101 76-77 1.5113187025917973 1101 78-79 1.2445053187758397 1101 80-81 0.7980453990852538 1101 82-83 1.674438967633403 1101 84-85 2.505074154240429 1101 86-87 0.45754354055216595 1101 88-89 1.5765061797566666 1101 90-91 2.413084653918503 1101 92-93 0.9514527921255116 1101 94-95 1.5138671824883012 1101 96-97 1.398673511078531 1101 98-99 1.8555576956702957 1101 100-101 1.7353561328278921 1102 1 -1.1039558535186558 1102 2 -0.7920218913731638 1102 3 -1.1877060487082645 1102 4 -1.4955908886885254 1102 5 -1.3836094215328174 1102 6 -0.9296108739070661 1102 7 -0.4900782724860733 1102 8 -0.6910491305078423 1102 9 -0.937254557277754 1102 10-11 -0.34916857597158923 1102 12-13 -0.4735687976406311 1102 14-15 -0.028654807544441496 1102 16-17 -0.21242389366431524 1102 18-19 -0.2411495027659214 1102 20-21 -0.2940946246605023 1102 22-23 0.3840170579390687 1102 24-25 0.23011119001820646 1102 26-27 -0.04668852989712491 1102 28-29 0.7296045740528072 1102 30-31 0.9895749642402336 1102 32-33 1.2982313908417566 1102 34-35 1.482611334531022 1102 36-37 1.764562118414922 1102 38-39 1.6408398304063283 1102 40-41 0.981300930216598 1102 42-43 1.0351205923828033 1102 44-45 1.3625220055398088 1102 46-47 1.727349529171562 1102 48-49 0.28407444694081363 1102 50-51 1.5215722594317782 1102 52-53 1.340428772805648 1102 54-55 0.9204286478433232 1102 56-57 2.1675888138543726 1102 58-59 1.2568121152043545 1102 60-61 0.8291228607935146 1102 62-63 0.7747502752117619 1102 64-65 1.3163735949292992 1102 66-67 0.7630424791054153 1102 68-69 1.403547508373748 1102 70-71 2.115077406420273 1102 72-73 1.8294768078342187 1102 74-75 1.1591478733204177 1102 76-77 1.490084728232823 1102 78-79 1.2897777546732776 1102 80-81 1.0252088606237137 1102 82-83 0.6968748650693009 1102 84-85 0.8223818465481223 1102 86-87 1.2339858482444725 1102 88-89 0.60895810283359 1102 90-91 1.514046192380043 1102 92-93 1.6177188177665371 1102 94-95 1.3620242337703523 1102 96-97 0.6406606905657135 1102 98-99 1.3078814136190147 1102 100-101 1.2321510046227644 1103 1 1.2037364541736544 1103 2 0.9002858009345296 1103 3 0.5302426692404545 1103 4 0.4018450087473724 1103 5 0.5907495528261606 1103 6 0.0960301517339559 1103 7 -0.07982186222966448 1103 8 0.18074574128702636 1103 9 0.6268480068248081 1103 10-11 0.20211347531046187 1103 12-13 0.4751491510773178 1103 14-15 0.714934936045303 1103 16-17 0.8132171319767068 1103 18-19 0.8614145997981808 1103 20-21 1.1930848625189796 1103 22-23 1.0122221861442 1103 24-25 0.7685727284797466 1103 26-27 1.1712601880515976 1103 28-29 0.9860148304630556 1103 30-31 1.0408570155222918 1103 32-33 0.7597698523802094 1103 34-35 1.3672267191464087 1103 36-37 1.251741605594404 1103 38-39 0.9613526509191459 1103 40-41 1.6095060584217293 1103 42-43 1.6633257205879346 1103 44-45 0.7855989286167286 1103 46-47 0.6376059394279707 1103 48-49 0.19433085719722243 1103 50-51 -0.42714568928616714 1103 52-53 -0.5954686630917898 1103 54-55 -0.5795713521566768 1103 56-57 0.05220419846975943 1103 58-59 -0.32011096171871856 1103 60-61 -0.3247232930526387 1103 62-63 -0.7893522888908002 1103 64-65 -0.6323443537886497 1103 66-67 -0.46772675166381106 1103 68-69 0.09585520068144149 1103 70-71 -0.5131277217848584 1103 72-73 -0.9141129357555222 1103 74-75 -0.687005972833429 1103 76-77 -1.0868383486902538 1103 78-79 -0.5179145530190326 1103 80-81 0.48674732216217365 1103 82-83 0.19687486506930085 1103 84-85 0.30956133372760775 1103 86-87 0.43911405337267695 1103 88-89 1.0448555387310243 1103 90-91 -0.02441534608149709 1103 92-93 0.027975228022949494 1103 94-95 1.7979216696677902 1103 96-97 0.8970709469759655 1103 98-99 -0.17929807356047434 1103 100-101 -0.690925918454159 1104 1 -0.022984193599626224 1104 2 -0.11995711404523135 1104 3 0.024844558579186327 1104 4 0.6089975053735586 1104 5 0.27698437063992287 1104 6 -0.4390575675642907 1104 7 -0.433398110542754 1104 8 -0.11210176208678746 1104 9 0.28339321600159906 1104 10-11 -0.2820295746220651 1104 12-13 0.025081674694050093 1104 14-15 -0.2597614202299994 1104 16-17 -0.35817288151857696 1104 18-19 -0.25768121666605737 1104 20-21 0.03046677884827176 1104 22-23 0.3266621321630936 1104 24-25 0.8775470874541043 1104 26-27 0.44150310303135143 1104 28-29 0.678997286603412 1104 30-31 0.4082659224048797 1104 32-33 0.17609913713054937 1104 34-35 0.7245141685391161 1104 36-37 1.0867618485093828 1104 38-39 1.1216090611755547 1104 40-41 0.8983049787995938 1104 42-43 1.1252015640427189 1104 44-45 0.9671103996018857 1104 46-47 1.3012361688476801 1104 48-49 0.45006634977482207 1104 50-51 -0.483151087396827 1104 52-53 0.3023046162604395 1104 54-55 -0.6419870066775957 1104 56-57 -0.9454341281159344 1104 58-59 -1.4004078578050887 1104 60-61 -1.566626127060733 1104 62-63 -1.040701816556119 1104 64-65 -1.1404415197805555 1104 66-67 -1.1903988164411388 1104 68-69 -1.4250624781309753 1104 70-71 -0.8997673978982164 1104 72-73 -1.5871898588324456 1104 74-75 -2.181607862172161 1104 76-77 -1.5618720868818876 1104 78-79 -1.330330207539948 1104 80-81 -1.765276969335801 1104 82-83 -2.279508400787652 1104 84-85 -1.4883468983911499 1104 86-87 -0.8368643541846765 1104 88-89 -1.4456977676117546 1104 90-91 -1.524415346081497 1104 92-93 -1.1316064184008034 1104 94-95 -0.8603104490906475 1104 96-97 -0.7098791205004176 1104 98-99 -0.528488356961283 1104 100-101 -0.060696498751052985 1105 1 0.009597260034457378 1105 2 -0.40191200126327686 1105 3 0.30679944579723184 1105 4 -0.11280700590464221 1105 5 -1.4147449526683502 1105 6 -0.6658746101708033 1105 7 -1.0724958549036572 1105 8 -0.7148586543173678 1105 9 -0.22663184665504588 1105 10-11 0.07573984893683416 1105 12-13 -0.17228674635858 1105 14-15 -0.020413049302682396 1105 16-17 0.20699002574959735 1105 18-19 0.0180080063915824 1105 20-21 0.3011434705775926 1105 22-23 0.3373137612357766 1105 24-25 0.06802327793029406 1105 26-27 0.6758389426303495 1105 28-29 0.4383957828440188 1105 30-31 0.6507471254123942 1105 32-33 0.30830465091501225 1105 34-35 0.7783988803185693 1105 36-37 0.15192475577755715 1105 38-39 1.2168471564136496 1105 40-41 1.5518137507294227 1105 42-43 1.838234145496358 1105 44-45 2.602448745466546 1105 46-47 2.4672762690983063 1105 48-49 3.2025726154389886 1105 50-51 2.0582022960618147 1105 52-53 2.2653371977140786 1105 54-55 2.2803187577334327 1105 56-57 2.248174894440453 1105 58-59 2.2668853752776172 1105 60-61 2.7485367802074343 1105 62-63 2.3452630957245866 1105 64-65 2.8420146205703283 1105 66-67 3.0652402813032182 1105 68-69 3.343107947934186 1105 70-71 3.2148942562371197 1105 72-73 2.49614347450089 1105 74-75 2.500722964895509 1105 76-77 2.143015131163228 1105 78-79 2.711023175918694 1105 80-81 2.468432303847159 1105 82-83 2.9184865866810235 1105 84-85 2.9157884399547136 1105 86-87 2.8072459215045455 1105 88-89 2.388262132137619 1105 90-91 2.880346558680408 1105 92-93 3.1277920778397963 1105 94-95 3.096456468202586 1105 96-97 2.6635544634594837 1105 98-99 1.7804088861464855 1105 100-101 2.0151180375897972 1106 1 0.6857877362249347 1106 2 0.24729434794307537 1106 3 0.7353708743686589 1106 4 0.10782791473027942 1106 5 0.4249375870141918 1106 6 0.7849190406228459 1106 7 0.7782977958899977 1106 8 0.13117309171438052 1106 9 0.3892411692179678 1106 10-11 0.22177159496857968 1106 12-13 0.23326880919697146 1106 14-15 0.38672980784017597 1106 16-17 0.39270431146388063 1106 18-19 0.4315000698836471 1106 20-21 0.372572042006162 1106 22-23 0.7968375707595854 1106 24-25 -0.10578624587923002 1106 26-27 0.9575849743763811 1106 28-29 0.6193481637963956 1106 30-31 -0.3317925571272866 1106 32-33 0.0725903652007247 1106 34-35 -0.5319185799988944 1106 36-37 -0.05680540295259817 1106 38-39 -0.8172798277133353 1106 40-41 -0.20929736038169722 1106 42-43 -0.010178552916336514 1106 44-45 0.4992741422919451 1106 46-47 0.686323888145921 1106 48-49 0.3763821392485056 1106 50-51 0.35661499447451206 1106 52-53 0.911368943745817 1106 54-55 1.2477790751937476 1106 56-57 1.529127275392831 1106 58-59 -0.07994002154777746 1106 60-61 0.10091773258838899 1106 62-63 -0.27140357094208056 1106 64-65 0.40868128723699115 1106 66-67 -0.061743845680908294 1106 68-69 -0.13149522666898505 1106 70-71 -0.17637558503271933 1106 72-73 0.19614347450088587 1106 74-75 0.09675471092725374 1106 76-77 -0.6466674085193134 1106 78-79 -0.34611968122416314 1106 80-81 -0.3029962675814133 1106 82-83 -0.3783388101443741 1106 84-85 -0.014370290204016811 1106 86-87 -0.6737064594478355 1106 88-89 -1.080785486909999 1106 90-91 -1.53552645719261 1106 92-93 -1.0856999856522656 1106 94-95 -0.22259115084503023 1106 96-97 -0.6388264889214703 1106 98-99 -0.6878450821074829 1106 100-101 -1.0285327560609936 1107 1 -0.1343673025347556 1107 2 0.18114447714204118 1107 3 0.20048715343842716 1107 4 -0.2606475374660988 1107 5 0.002198568926338851 1107 6 -0.19854349167689378 1107 7 -0.06252907749501446 1107 8 0.029881102050296704 1107 9 -1.4148931977070944 1107 10-11 -0.5981250458582892 1107 12-13 -1.003682095195785 1107 14-15 -0.8977663161908325 1107 16-17 -1.3832646807841797 1107 18-19 -0.5431769327003337 1107 20-21 -0.7320791207845403 1107 22-23 -0.42486785559700735 1107 24-25 -0.08175523812729324 1107 26-27 -0.8072729067605664 1107 28-29 -0.8240626889167828 1107 30-31 -1.0253326088068704 1107 32-33 -0.9328359913884228 1107 34-35 -0.8259754275441153 1107 36-37 -0.409260183314359 1107 38-39 -0.360949078359333 1107 40-41 -0.06252733454189752 1107 42-43 -0.5042354004615603 1107 44-45 -0.26299975951684473 1107 46-47 0.43128512845599687 1107 48-49 1.476382139248507 1107 50-51 0.17780362496547042 1107 52-53 1.2193792796631335 1107 54-55 1.792223519638192 1107 56-57 0.9508327017494267 1107 58-59 1.1265199267726302 1107 60-61 1.3792123062317962 1107 62-63 1.4014646461121778 1107 64-65 2.1637200469269153 1107 66-67 2.5868349656886025 1107 68-69 2.3442153676462567 1107 70-71 2.016389272848418 1107 72-73 1.5465310714001106 1107 74-75 1.2822844266895324 1107 76-77 0.48769951654528754 1107 78-79 0.6562059001711908 1107 80-81 0.07529830606199539 1107 82-83 0.07850873507526401 1107 84-85 1.013795084472985 1107 86-87 1.6239679591568148 1107 88-89 2.325416063477597 1107 90-91 1.0337241888022248 1107 92-93 0.5455661642185348 1107 94-95 0.8107421824883012 1107 96-97 0.519313045962253 1107 98-99 0.10156060264704081 1107 100-101 0.023146830502309257 1108 1 1.8599373960888776 1108 2 1.2001288150632554 1108 3 1.2863912825319268 1108 4 1.285152177768829 1108 5 1.6974999452908364 1108 6 1.4701798115979017 1108 7 1.2132184308106275 1108 8 1.104869236839093 1108 9 1.3311912825966559 1108 10-11 1.3342432502973836 1108 12-13 1.4756724373148842 1108 14-15 1.5799270867517379 1108 16-17 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0.17833216619168724 2314 52-53 0.577025509402386 2314 54-55 0.610405337820012 2314 56-57 0.1518545481201059 2314 58-59 1.0872316956239416 2314 60-61 0.7236450053156638 2314 62-63 0.39071764117912977 2314 64-65 0.2738328023885046 2314 66-67 -0.6804307143677732 2314 68-69 -0.16987906505282524 2314 70-71 -0.2829412415983761 2314 72-73 0.6628101411675544 2314 74-75 -0.4678917537192113 2314 76-77 0.7220194601675551 2314 78-79 0.5735772884728085 2314 80-81 -0.9136023281874728 2314 82-83 -0.5414701232756869 2314 84-85 -1.2714409972747198 2314 86-87 0.0929602072188338 2314 88-89 0.6631539070293933 2314 90-91 0.2255846539185029 2314 92-93 0.576926276973996 2314 94-95 0.7046815764276957 2314 96-97 1.2566280565330779 2314 98-99 1.2187205744581746 2314 100-101 1.542174314646072 2315 1 -0.1650059145687166 2315 2 -0.235245334596609 2315 3 -0.20113706213927784 2315 4 0.21258981949218736 2315 5 0.7201756822522896 2315 6 0.08015713586094364 2315 7 0.2608374784296785 2315 8 -0.5323189717776842 2315 9 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2315 74-75 -1.4953087811362344 2315 76-77 -2.0752388370907404 2315 78-79 -2.169929205033686 2315 80-81 -3.1664883310734773 2315 82-83 -2.097386429191996 2315 84-85 -2.044529020362745 2315 86-87 -2.2879921737335494 2315 88-89 -2.5601505662750803 2315 90-91 -2.373621695287845 2315 92-93 -2.3682396681919506 2315 94-95 -2.157511785765667 2315 96-97 -1.4118423619373424 2315 98-99 -2.362448256710657 2315 100-101 -0.9967867243149655 2316 1 0.291343291780489 2316 2 0.5584054590541854 2316 3 0.8464819854797732 2316 4 0.7800501369525037 2316 5 0.16660425368085896 2316 6 -0.5011920704882655 2316 7 0.1755200181122163 2316 8 0.5867286472699362 2316 9 0.7475745025513021 2316 10-11 0.8564938171908025 2316 12-13 0.9943799203080914 2316 14-15 -0.42715908104871403 2316 16-17 -0.7364623552027822 2316 18-19 -0.043499930116354335 2316 20-21 0.12951648645060487 2316 22-23 0.16072645964847254 2316 24-25 0.41921375412076856 2316 26-27 0.277029418820824 2316 28-29 0.13879260824084128 2316 30-31 -0.2540147793495109 2316 32-33 -0.20657630146593675 2316 34-35 -0.3971963577766715 2316 36-37 -0.9651387362859367 2316 38-39 -1.4270020499355596 2316 40-41 -0.6217973603816915 2316 42-43 -1.1310118862496736 2316 44-45 -0.743781413263612 2316 46-47 -0.6775650007429661 2316 48-49 0.06665991702627849 2316 50-51 0.5621705500300749 2316 52-53 0.46275783263471126 2316 54-55 0.5977790751937491 2316 56-57 0.8791272753928396 2316 58-59 0.6200599784522183 2316 60-61 0.2425843992550547 2316 62-63 0.3244297623912509 2316 64-65 0.8281257316814354 2316 66-67 0.486867265430206 2316 68-69 0.3837825511087942 2316 70-71 0.38056885941172425 2316 72-73 0.6072545856119973 2316 74-75 0.39953248870503444 2316 76-77 -0.014722964074866951 2316 78-79 1.5274914298869504 2316 80-81 1.209503732418586 2316 82-83 1.2647167454111816 2316 84-85 0.8870185986848718 2316 86-87 0.926293540552166 2316 88-89 0.8664367353122238 2316 90-91 1.03114020947406 2316 92-93 0.8073555699032902 2316 94-95 0.8454644047105226 2316 96-97 1.530617955522974 2316 98-99 1.478821584559185 2316 100-101 1.554800577272335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 206.0 8 496.0 9 348.0 10 296.0 11 329.0 12 356.0 13 312.0 14 276.0 15 333.0 16 439.0 17 607.0 18 804.0 19 1188.0 20 1418.0 21 1554.0 22 1103.0 23 902.0 24 744.0 25 682.0 26 595.0 27 646.0 28 662.0 29 622.0 30 760.0 31 734.0 32 930.0 33 1149.0 34 1564.0 35 3632.0 36 4187.0 37 8097.0 38 10802.0 39 2301.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.55976854587315 21.325971353476906 14.55756810170942 17.556691998940526 2 17.67893889692549 24.16821173162731 38.36311403598134 19.789735335465863 3 18.830097186283897 30.967176707891035 32.845704040259974 17.357022065565086 4 11.59715572217355 22.976304476273913 37.18139402212669 28.245145779425844 5 9.891811495283308 40.172368126158794 34.72423137262892 15.211589005928975 6 23.36953199812555 39.8463763981989 18.161814143966097 18.622277459709462 7 20.93885617652452 40.351663576536744 22.660499989812756 16.048980257125976 8 24.23137262891954 33.32042949410159 21.519528941953098 20.928668935025772 9 26.708909761414805 17.50575579144679 21.727348668527537 34.05798577861086 10-11 22.56575864387441 28.513070230842892 27.12556793871356 21.79560318656914 12-13 25.536358264909026 30.22147063018276 27.196878629204786 17.04529247570343 14-15 22.425174711191705 30.44253377070557 28.62512988732911 18.50716163077362 16-17 17.19097002913551 34.19245736639433 29.128379617367212 19.488192987102952 18-19 16.934251543367086 31.430696196084025 34.41861412766651 17.216438132882377 20-21 18.341109594344044 30.820480430309082 34.47362523175974 16.364784743587133 22-23 18.164870316415723 31.106741916423868 33.66679570505898 17.061592062101425 24-25 17.422220411157067 32.44330800105947 33.55575477272264 16.57871681506082 26-27 17.17161427028789 31.391984678388784 34.168007986797335 17.268393064525988 28-29 17.06872313115055 31.309468022248936 35.191825757421405 16.42998308917911 30-31 17.60559075813451 32.02664982376072 34.25154336708706 16.116216051017705 32-33 16.43609543407836 32.255862757482525 35.16839510197428 16.139646706464823 34-35 16.482956744972597 32.4219147939121 34.04983598541187 17.04529247570343 36-37 16.87210937022473 32.408671379963735 33.55881094517227 17.16040830463927 38-39 16.978056681811697 32.38829689696624 33.49870622032966 17.1349402008924 40-41 16.6520649538518 32.47183227725596 34.061041951060496 16.815060817831746 42-43 16.62048450520568 32.824310833112605 33.2674558383081 17.287748823373608 44-45 16.911839612069844 31.671115095454454 33.38664656384344 18.03039872863226 46-47 17.382490169311954 31.514231576373746 32.53499317454819 18.5682850797661 48-49 17.29080499582323 31.46635154132964 32.549255312646444 18.69358815020069 50-51 17.23681261587987 30.944764776593793 32.451457794258474 19.366964813267863 52-53 17.23477516758012 31.17601515861535 32.446364173509096 19.14284550029543 54-55 17.53835496424278 30.874472810252442 32.083698376153706 19.50347384935107 56-57 17.243943684928993 30.487357633300054 32.26604999898128 20.002648682789676 58-59 17.401845928159574 30.073755628450925 32.235488274485036 20.288910168904465 60-61 17.430370204356063 29.975958110062955 32.32615472382388 20.267516961757096 62-63 17.126790407693406 29.660153623601804 32.625659623887046 20.58739634481775 64-65 16.717263299443776 29.87612314337524 32.869134695707096 20.53747886147389 66-67 16.81811699028137 29.5674497259632 32.08471710030358 21.529716183451843 68-69 16.85479105967686 29.446221552128115 32.25178786088303 21.447199527311994 70-71 16.788573989935003 29.464558586825863 32.22326358468654 21.523603838552596 72-73 17.625965241132004 28.615961369980237 31.83105478698478 21.927018601902976 74-75 17.315254375420224 28.849249200301543 31.74751940669506 22.087977017583178 76-77 17.518999205395165 28.585399645483996 31.62221633626047 22.27338481286037 78-79 17.44666979075406 28.006764328355167 31.667040198854956 22.87952568203582 80-81 17.705425724822234 28.009820500804793 31.511175403924124 22.77357837044885 82-83 17.639208655080377 28.051588190949655 31.10877936472362 23.200423789246347 84-85 17.12984658014303 27.600293392555166 31.006906949736152 24.26295307756566 86-87 17.735987449318475 27.69910963509301 30.64016625578126 23.92473665980726 88-89 18.097634522523993 27.124549214563682 30.47207677105194 24.305739491860397 90-91 17.940751003443285 26.98090910943135 31.22185774535971 23.856482141765653 92-93 18.330922352845295 26.889223935942624 31.24325095250708 23.536602758704998 94-95 18.332959801145048 26.35846865385791 31.46635154132964 23.842220003667407 96-97 18.517348872272365 25.940791752409282 31.419490230435404 24.12236914488295 98-99 18.689513253601188 26.23214685927345 30.98245757013916 24.095882316986206 100-101 19.350665226869868 25.047370672969173 31.555999266518615 24.045964833642348 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 910.0 1 556.5 2 204.0 3 232.0 4 177.0 5 116.0 6 202.5 7 403.0 8 518.5 9 463.0 10 335.0 11 205.5 12 185.5 13 193.0 14 182.5 15 169.0 16 165.5 17 175.5 18 172.5 19 176.0 20 190.5 21 214.0 22 246.5 23 279.0 24 319.0 25 391.5 26 512.5 27 632.0 28 690.0 29 779.0 30 910.0 31 1040.5 32 1216.0 33 1381.5 34 1531.0 35 1609.5 36 1650.0 37 1727.0 38 1681.0 39 1516.5 40 1423.0 41 1386.5 42 1314.5 43 1279.5 44 1277.0 45 1185.0 46 1134.5 47 1271.5 48 1655.5 49 1849.5 50 1727.0 51 1536.0 52 1239.5 53 1038.0 54 877.0 55 720.0 56 611.0 57 510.5 58 490.0 59 448.0 60 351.0 61 303.5 62 267.5 63 193.0 64 144.5 65 146.0 66 114.5 67 95.5 68 91.5 69 83.0 70 83.0 71 66.5 72 60.5 73 70.0 74 57.5 75 42.0 76 30.5 77 15.5 78 14.5 79 15.0 80 10.5 81 8.5 82 6.5 83 5.0 84 4.5 85 4.0 86 3.5 87 3.5 88 3.0 89 1.5 90 2.0 91 1.5 92 0.0 93 1.0 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 49081.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.39006947698702 #Duplication Level Percentage of deduplicated Percentage of total 1 79.58140219230486 44.08019396507814 2 9.799161333039063 10.85552454106477 3 3.9248142426248807 6.5218720074978105 4 2.0635621275656586 4.57203398463764 5 1.2653571691311705 3.5044110755689575 6 0.731994408886927 2.432713269900776 7 0.6473920400205988 2.5101363052912533 8 0.33105274773780624 1.4669627758195636 9 0.2574854704627382 1.283592428842118 >10 1.3205326270874715 11.743851999755506 >50 0.02942691091002722 1.0350237362726922 >100 0.03310527477378062 3.785578940934374 >500 0.01471345545501361 6.208104969336404 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 1.7399808479859824 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 838 1.707381675189992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 781 1.5912471221042765 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 574 1.169495324056152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 459 0.9351887695849718 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 344 0.7008822151137916 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.5501110409323363 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.39526497015138246 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.31376703816140666 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 116 0.23634400277092968 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.2322691061714309 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.21393207147368637 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.20781972657443817 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 82 0.16707076057945028 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.1385464843829588 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 67 0.13650903608320938 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 62 0.12632179458446244 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 61 0.12428434628471302 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.11613455308571545 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 56 0.11409710478596606 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.11205965648621666 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.001018724149874697 0.0 16-17 0.0 0.0 0.0 0.002037448299749394 0.0 18-19 0.0 0.0 0.0 0.002037448299749394 0.0 20-21 0.0 0.0 0.0 0.002037448299749394 0.0 22-23 0.0 0.0 0.0 0.014262138098245756 0.0 24-25 0.0 0.0 0.0 0.03871151769523848 0.0 26-27 0.0 0.0 0.0 0.09168517348872272 0.0 28-29 0.0 0.0 0.0 0.20374482997493937 0.0 30-31 0.0 0.0 0.0 0.38711517695238484 0.0 32-33 0.0 0.0 0.0 0.5725229722295797 0.0 34-35 0.0 0.0 0.0 0.8455410443959984 0.0 36-37 0.0 0.0 0.0 1.1368961512601619 0.0 38-39 0.0 0.0 0.0 1.428251258124325 0.0 40-41 0.0 0.0 0.0 1.8601902976711966 0.0 42-43 0.0 0.0 0.0 2.1749760599824777 0.0 44-45 0.0 0.0 0.0 2.4561439253478943 0.0 46-47 0.0 0.0 0.0 2.742405411462684 0.0 48-49 0.0 0.0 0.0 3.035797966626597 0.0 50-51 0.0 0.0 0.0 3.300666245594018 0.0 52-53 0.0 0.0 0.0 3.549234938163444 0.0 54-55 0.0 0.0 0.0 3.804934699781993 0.0 56-57 0.0 0.0 0.0 4.197143497483751 0.0 58-59 0.0 0.0 0.0 4.498685845846662 0.0 60-61 0.0 0.0 0.0 4.786984780261201 0.0 62-63 0.0 0.0 0.0 5.09260202522361 0.0 64-65 0.0 0.0 0.0 5.36154520079053 0.0 66-67 0.0 0.0 0.0 5.673274790652187 0.0 68-69 0.0 0.0 0.0 6.087895519651189 0.0 70-71 0.0 0.0 0.0 6.572808214991545 0.0 72-73 0.0 0.0 0.0 6.996597461339419 0.0 74-75 0.0 0.0 0.0 7.439742466534911 0.0 76-77 0.0 0.0 0.0 7.8676066094822845 0.0 78-79 0.0 0.0 0.0 8.33927589087427 0.0 80-81 0.0 0.0 0.0 8.872068621258736 0.0 82-83 0.0 0.0 0.0 9.377355799596586 0.0 84-85 0.0 0.0 0.0 9.862268494936941 0.0 86-87 0.0 0.0 0.0 10.346162466127423 0.0 88-89 0.0 0.0 0.0 10.86163688596402 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCCAT 20 0.0020622227 71.25 9 CGCAGAA 180 0.0 63.333332 9 TATATGG 25 0.0049927398 57.0 4 GGAGTTC 25 0.0049927398 57.0 6 AGTTCCA 25 0.0049927398 57.0 8 ACAGAGC 25 0.0049927398 57.0 8 GGTATCA 825 0.0 42.030304 1 CAGGAGT 50 0.0016007768 38.0 4 GTTCAGG 50 0.0016007768 38.0 1 CTCCTGA 50 0.0016007768 38.0 1 GCCGTAT 40 1.0676036E-5 35.625 94-95 CAGAAAA 215 0.0 33.139534 10-11 GTATCAA 1055 0.0 32.8673 2 AACGCAG 1125 0.0 31.244444 7 TATCAAC 1150 0.0 30.152174 3 CAACGCA 1180 0.0 29.788136 6 TCAACGC 1185 0.0 29.662447 5 ACGCAGA 1200 0.0 29.291668 8 ATCAACG 1215 0.0 28.539095 4 TCGCCGT 50 4.9076996E-5 28.5 92-93 >>END_MODULE