FastQCFastQC Report
Fri 10 Feb 2017
SRR2031847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49081
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT18313.73056783684114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9972.0313359548501455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8471.7257187098877365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8391.7094191234897416No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6821.3895397404290866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5071.0329862879729428No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT4820.9820500804792078RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT3540.7212566981112855RNA PCR Primer, Index 46 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.6560583525193048No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.5236242130355943No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.4013773150506306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.3545160041563945No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.2669057272671706No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT1220.24856869256942604RNA PCR Primer, Index 46 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.21189462317393695No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.21189462317393695No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT940.191520140176443No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG840.17114565717894908TruSeq Adapter, Index 4 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.16299586397995153No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.14873372588170575No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG730.14873372588170575No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.13447158778346No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT610.12428434628471302No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT590.12020944968521424No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC580.11817200138546484No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG550.11205965648621666RNA PCR Primer, Index 46 (96% over 28bp)
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG550.11205965648621666No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA530.10798475988671788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG510.10390986328721909No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.10390986328721909No Hit
GTTCAGGAGTGATCTGGGCACAGAAGCCGAAGTCAGTAAGTTTAACTGAG500.1018724149874697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCT200.002062398271.2484749
GTTTAGA250.00495293157.1152231
TTATAGC250.00499316356.9987758
GAAAGCG352.76194E-454.2845469
ACCCATG352.76194E-454.2845463
TAAAGAA405.3381466E-447.498985
TACCCAT459.5360976E-442.2213132
TTGGAGT459.5360976E-442.2213134
GTACCCA500.001584941938.0768171
TACGAAT250.001608789237.99918434-35
CGCAGAA1054.504909E-836.18979
GGTATCA7750.035.6202471
AGTATGA300.003933926631.66598770-71
TATGAGT300.003933926631.66598772-73
GCAGAGA650.005791010329.230149
GTATCAA11050.028.428391
TTAGTTC350.00835573927.14227366-67
CGCAGAG11800.026.1646928
AACGCAG12000.026.1244416
TCAACGC12450.025.1801824
Produced by FastQC (version 0.11.5)