##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031847_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49081 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64900878140217 34.0 31.0 34.0 31.0 34.0 2 32.738147144516205 34.0 31.0 34.0 31.0 34.0 3 32.70291966341354 34.0 31.0 34.0 31.0 34.0 4 36.197347242313725 37.0 35.0 37.0 35.0 37.0 5 36.22579002057823 37.0 37.0 37.0 35.0 37.0 6 36.358652024204886 37.0 37.0 37.0 35.0 37.0 7 36.29705996210346 37.0 37.0 37.0 35.0 37.0 8 36.348709276502106 37.0 37.0 37.0 35.0 37.0 9 38.09818463356493 39.0 39.0 39.0 37.0 39.0 10-11 38.102840202929855 39.0 39.0 39.0 37.0 39.0 12-13 38.10176035533098 39.0 39.0 39.0 37.0 39.0 14-15 39.47632485075691 41.0 39.5 41.0 36.5 41.0 16-17 39.392677410810705 41.0 39.0 41.0 36.5 41.0 18-19 39.469326215847275 41.0 39.5 41.0 36.0 41.0 20-21 39.502098571748746 41.0 39.0 41.0 36.0 41.0 22-23 39.3967217456857 41.0 39.0 41.0 35.0 41.0 24-25 39.24936329740633 41.0 39.0 41.0 35.0 41.0 26-27 39.000244493795975 41.0 38.0 41.0 35.0 41.0 28-29 38.793861168272855 40.5 38.0 41.0 35.0 41.0 30-31 38.405411462684135 40.0 37.0 41.0 35.0 41.0 32-33 37.59160367555673 40.0 35.0 41.0 33.5 41.0 34-35 37.44321631588599 40.0 35.0 41.0 33.0 41.0 36-37 37.25743159267334 40.0 35.0 41.0 33.0 41.0 38-39 36.76159817444632 39.0 35.0 41.0 33.0 41.0 40-41 36.59583138077871 39.0 35.0 41.0 32.5 41.0 42-43 36.17741081070068 39.5 35.0 41.0 30.5 41.0 44-45 35.58347425684073 39.0 35.0 41.0 25.0 41.0 46-47 34.988885719524866 39.0 35.0 41.0 21.0 41.0 48-49 34.41042358550152 39.0 35.0 41.0 12.5 41.0 50-51 33.89326827081763 39.0 35.0 41.0 10.0 41.0 52-53 33.88581121004054 39.0 35.0 41.0 10.0 41.0 54-55 33.69944581406247 38.5 35.0 41.0 9.0 41.0 56-57 33.39369613496058 38.0 34.0 41.0 8.0 41.0 58-59 33.16542042745665 37.5 34.0 41.0 8.0 41.0 60-61 32.76805688555653 37.0 33.5 41.0 7.0 41.0 62-63 32.342484871946375 36.5 33.0 40.0 7.0 41.0 64-65 31.901998736782055 36.0 32.5 40.0 7.0 41.0 66-67 31.349860434791466 35.5 30.5 39.0 7.0 41.0 68-69 30.533648458670363 35.0 27.5 39.0 7.0 41.0 70-71 29.822253010329863 35.0 24.0 38.0 7.0 41.0 72-73 29.20124895580775 35.0 20.0 37.0 7.0 39.5 74-75 28.806350726350317 35.0 20.0 37.0 7.0 39.0 76-77 27.699649558892446 33.5 19.5 35.5 7.0 38.0 78-79 28.256779609217418 35.0 20.0 36.0 7.0 37.5 80-81 28.124498278356185 35.0 19.5 35.5 7.0 37.0 82-83 27.902528473339988 35.0 19.0 35.0 7.0 37.0 84-85 27.738422200036673 35.0 19.0 35.0 7.0 36.0 86-87 27.526812819624702 35.0 18.5 35.0 7.0 36.0 88-89 27.345072431287058 35.0 18.0 35.0 7.0 36.0 90-91 27.1381899309305 35.0 17.0 35.0 7.0 35.5 92-93 27.001232656221347 35.0 15.5 35.0 7.0 35.0 94-95 26.906226442004034 35.0 15.0 35.0 7.0 35.0 96-97 26.82072492410505 35.0 15.0 35.0 7.0 35.0 98-99 26.7260447016157 35.0 15.0 35.0 7.0 35.0 100-101 25.849269574784543 33.0 12.5 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5003977471901209 1101 2 0.8334184025983902 1101 3 0.9299511018554867 1101 4 0.4319313346423357 1101 5 0.36170321116026827 1101 6 0.42868887154634905 1101 7 0.394603977115203 1101 8 0.3083062207999703 1101 9 0.4565774167187939 1101 10-11 0.07764276400573777 1101 12-13 0.4244331025894894 1101 14-15 0.8543875347113925 1101 16-17 0.979832730673678 1101 18-19 0.13708952155107568 1101 20-21 0.3907609290297174 1101 22-23 0.4868535516281156 1101 24-25 0.780975362669686 1101 26-27 0.9965846237592686 1101 28-29 1.0785217120176824 1101 30-31 0.7871145657643908 1101 32-33 0.9512749913086935 1101 34-35 1.427930510246405 1101 36-37 1.4059011217991824 1101 38-39 1.1744737209862208 1101 40-41 1.0220954253227035 1101 42-43 1.849221408026736 1101 44-45 2.3741255642770014 1101 46-47 -0.013561682298053768 1101 48-49 0.32018015603529193 1101 50-51 1.1538429289361574 1101 52-53 1.1307639110321546 1101 54-55 1.9680861951358466 1101 56-57 1.8402797482730122 1101 58-59 0.8582835393941508 1101 60-61 1.5418194751326553 1101 62-63 1.2102361046890096 1101 64-65 1.073069213825363 1101 66-67 1.4157865212693004 1101 68-69 0.9553841501257381 1101 70-71 1.1204703511480893 1101 72-73 0.08106889093023639 1101 74-75 -0.4692951703286745 1101 76-77 0.24966531650391133 1101 78-79 0.29891720267267985 1101 80-81 0.13595126881616793 1101 82-83 0.20826470265582486 1101 84-85 0.7076078332337978 1101 86-87 0.05551821066704221 1101 88-89 -0.19737701425576404 1101 90-91 1.3656781849395827 1101 92-93 0.8091466371326099 1101 94-95 0.7505355273812313 1101 96-97 1.1835352352546664 1101 98-99 0.831603215446787 1101 100-101 0.45360880899648137 1102 1 0.40664774719012087 1102 2 -0.10808800765801863 1102 3 -0.9258181289137433 1102 4 -0.013581485870481913 1102 5 -0.14951473755768063 1102 6 -0.5889393335818554 1102 7 0.2712065412177651 1102 8 0.3756139131076637 1102 9 0.35000690389828293 1102 10-11 0.17219404605702238 1102 12-13 0.3775581025894894 1102 14-15 0.015044585993443604 1102 16-17 -0.41640124368529285 1102 18-19 -0.019160478448924323 1102 20-21 0.38915836492714817 1102 22-23 0.04654906444862661 1102 24-25 0.11310677292609483 1102 26-27 0.5807192391438818 1102 28-29 0.48998004535101103 1102 30-31 0.7851113606361864 1102 32-33 0.9857301195138177 1102 34-35 1.0132670487079452 1102 36-37 0.20157419872226257 1102 38-39 0.5490730799605856 1102 40-41 -0.14096547211319432 1102 42-43 -0.49933628428095744 1102 44-45 0.1521704360718772 1102 46-47 0.703585753599377 1102 48-49 0.8914942585993941 1102 50-51 1.654644210987442 1102 52-53 1.5101709623142057 1102 54-55 2.0858746566743065 1102 56-57 1.4368342354525012 1102 58-59 1.3963444368300486 1102 60-61 1.7301207571839399 1102 62-63 1.615684822637725 1102 64-65 2.0862903676715163 1102 66-67 0.7463153674231471 1102 68-69 -0.5153690550024663 1102 70-71 -0.8518854180826807 1102 72-73 -0.660918288556946 1102 74-75 -1.4136060677645723 1102 76-77 -0.7883955809319865 1102 78-79 -0.5352174127119333 1102 80-81 -0.036324372209474376 1102 82-83 -1.2308378614467372 1102 84-85 -0.8388665257405599 1102 86-87 -0.05185358420475339 1102 88-89 0.4288249088211593 1102 90-91 0.6881942105806083 1102 92-93 -0.06545272184174777 1102 94-95 0.2285002709709758 1102 96-97 -0.6734359185914869 1102 98-99 -0.6776115281429576 1102 100-101 -0.8953495243368508 1103 1 0.6117759523183253 1103 2 0.4047325051624924 1103 3 0.3305921274965158 1103 4 0.11462364233464228 1103 5 0.28638269833975727 1103 6 0.25721451257199135 1103 7 0.1686424386536629 1103 8 0.17048570797945928 1103 9 0.5551351090264873 1103 10-11 0.4029632768262488 1103 12-13 0.4929427179741026 1103 14-15 0.3099163808652392 1103 16-17 0.6733423460582912 1103 18-19 0.3526343933459515 1103 20-21 0.6071070828758707 1103 22-23 0.226036243935809 1103 24-25 0.5361836960030146 1103 26-27 0.2986679570926043 1103 28-29 0.0028005581715291328 1103 30-31 0.31075238627720836 1103 32-33 0.10111473489843092 1103 34-35 -0.6533996179587263 1103 36-37 -0.8368873397392846 1103 38-39 0.15163718252468072 1103 40-41 -0.15378598493370532 1103 42-43 0.05194576700109366 1103 44-45 -0.2452654613640206 1103 46-47 -0.04000398999036037 1103 48-49 0.49405836116349633 1103 50-51 -0.819714763371536 1103 52-53 0.12555557769882597 1103 54-55 0.303823374623029 1103 56-57 0.6676034662217276 1103 58-59 0.9476264881120997 1103 60-61 0.12755665461983767 1103 62-63 0.9105566175095205 1103 64-65 1.2785980599792026 1103 66-67 1.207853828961607 1103 68-69 1.4077078680744535 1103 70-71 1.0070889408916806 1103 72-73 1.4416458140071597 1103 74-75 1.8043426501841466 1103 76-77 1.2628864703500646 1103 78-79 0.7211928436983222 1103 80-81 0.6944448585597591 1103 82-83 1.397367266758387 1103 84-85 0.7636975768235423 1103 86-87 1.1019925696414 1103 88-89 1.7621582421544915 1103 90-91 1.5856301080165025 1103 92-93 1.9217267653377377 1103 94-95 1.5490130914837934 1103 96-97 1.4034871583315898 1103 98-99 1.130080779549349 1103 100-101 1.1302915013041748 1104 1 0.2750687998217032 1104 2 0.24953682365102026 1104 3 0.19968794396074685 1104 4 0.3595629135897056 1104 5 0.43078215852868595 1104 6 0.30039939786213665 1104 7 0.12815660869414813 1104 8 -0.23774641077897485 1104 9 -0.08454100433383616 1104 10-11 0.021721711374162567 1104 12-13 0.24597915522106462 1104 14-15 0.10849937681665267 1104 16-17 -0.2841475324842122 1104 18-19 0.033471100498445594 1104 20-21 0.36115566587182 1104 22-23 0.17846539373337578 1104 24-25 -0.020833847856629006 1104 26-27 -0.2320337972933615 1104 28-29 0.22243618570189483 1104 30-31 -0.14133938160403403 1104 32-33 0.8575249913086935 1104 34-35 0.6458581418253502 1104 36-37 0.5851774375886549 1104 38-39 -0.7704275948032517 1104 40-41 -1.0724769430983514 1104 42-43 -0.6491338551311543 1104 44-45 -0.6275191725651013 1104 46-47 -1.021785366508574 1104 48-49 -1.2768593176489205 1104 50-51 -0.9168807552743701 1104 52-53 -1.6990058258099552 1104 54-55 -1.0072427522325782 1104 56-57 -0.39245051488487803 1104 58-59 -1.1195125132374244 1104 60-61 -0.7229831564462899 1104 62-63 -1.3259481058373055 1104 64-65 -1.1333452598588494 1104 66-67 -1.4945753208359598 1104 68-69 -2.033925060400577 1104 70-71 -1.4247599120098045 1104 72-73 -1.047220582753976 1104 74-75 -0.5951175387497294 1104 76-77 -1.0883281045487188 1104 78-79 -1.4066749025904777 1104 80-81 -0.9347724153943595 1104 82-83 -0.1272616131336477 1104 84-85 -0.5456816404504146 1104 86-87 -0.5727712630171702 1104 88-89 0.09785324890213332 1104 90-91 0.5836058165185278 1104 92-93 0.08299532134313736 1104 94-95 0.09017368527596759 1104 96-97 -1.006431870008491 1104 98-99 -0.9471796792900555 1104 100-101 -1.0883319804772036 1105 1 0.5733144138567852 1105 2 0.37359697402695957 1105 3 0.3525701494745377 1105 4 -0.2644972367862337 1105 5 0.5134889254459836 1105 6 0.30666506202253885 1105 7 0.4213896914009183 1105 8 0.13568717318091927 1105 9 0.4580655119568888 1105 10-11 0.4615713354343072 1105 12-13 0.4370819121132996 1105 14-15 0.3685244394732976 1105 16-17 0.42700534972129844 1105 18-19 0.12369666440821447 1105 20-21 0.46516569093447657 1105 22-23 0.8670618849614442 1105 24-25 0.750469410288737 1105 26-27 0.8462870047116482 1105 28-29 0.9066467120176824 1105 30-31 0.8830967086215296 1105 32-33 1.0956202294039343 1105 34-35 1.3676626531035438 1105 36-37 1.3508415979896569 1105 38-39 1.1781939590814616 1105 40-41 1.2921847110369953 1105 42-43 1.5024952175505462 1105 44-45 1.566089849991286 1105 46-47 1.255783555797187 1105 48-49 0.7889301560352919 1105 50-51 1.9440215003647268 1105 52-53 1.7565079586512056 1105 54-55 1.5648123856120364 1105 56-57 1.6282261768444428 1105 58-59 2.296527587013202 1105 60-61 2.2575932846564655 1105 62-63 2.731069438022338 1105 64-65 2.4086346900158446 1105 66-67 2.6025424736502494 1105 68-69 2.0469020072685993 1105 70-71 1.7506786844814215 1105 72-73 2.804283176644521 1105 74-75 2.9838298296713255 1105 76-77 2.5971355545991486 1105 78-79 2.5325481550536324 1105 80-81 2.5332726973875985 1105 82-83 2.6308837502748723 1105 84-85 2.3534411665671335 1105 86-87 2.885875353524188 1105 88-89 2.9938432238394768 1105 90-91 2.995886518272915 1105 92-93 3.0078073514183252 1105 94-95 2.580148622619326 1105 96-97 2.8732673781118123 1105 98-99 2.482644882113455 1105 100-101 2.7475076185202916 1106 1 0.13998108052345515 1106 2 0.1910572914872759 1106 3 -0.339493342588959 1106 4 -0.17084644313543862 1106 5 0.043647655604715396 1106 6 0.21618887154635047 1106 7 0.084881754892983 1106 8 -0.20558266808891545 1106 9 0.17393852782990393 1106 10-11 0.12347609733907206 1106 12-13 -0.4779974529660649 1106 14-15 -0.1584596875108275 1106 16-17 -0.5087089359929848 1106 18-19 0.5697284104399643 1106 20-21 0.6199275956963888 1106 22-23 -0.032938115038547267 1106 24-25 0.11396147378079746 1106 26-27 -0.11244315401851424 1106 28-29 -0.4655755102045376 1106 30-31 0.11246178798661077 1106 32-33 0.5464138801975835 1106 34-35 -0.02519448975359495 1106 36-37 0.29131778846584666 1106 38-39 -0.29879016790267343 1106 40-41 -0.7213073524550708 1106 42-43 -1.0403619253065983 1106 44-45 -1.4751799912785586 1106 46-47 -1.9767561267424938 1106 48-49 -2.0110698439647052 1106 50-51 -2.6940737377305126 1106 52-53 -2.4077777556345126 1106 54-55 -1.9867749159752606 1106 56-57 -2.2059008072825463 1106 58-59 -1.7455359050502928 1106 60-61 -1.7297083026451219 1106 62-63 -1.7244861175332105 1106 64-65 -1.723111341730192 1106 66-67 -2.388727367619591 1106 68-69 -1.799129738763149 1106 70-71 -1.3937657599630207 1106 72-73 -1.1036533312919836 1106 74-75 -1.482836836995343 1106 76-77 -1.1576263501627544 1106 78-79 -0.7198327973273173 1106 80-81 -0.7508542867393864 1106 82-83 -0.6556241862330623 1106 84-85 -0.12433661121064787 1106 86-87 0.9207959884448194 1106 88-89 1.161303541299791 1106 90-91 -0.30411348172708585 1106 92-93 -0.9064783628673929 1106 94-95 -0.5706450281743258 1106 96-97 -0.586256431412 1106 98-99 -0.8562440067754373 1106 100-101 0.026872697885369945 1107 1 -0.0584685318796474 1107 2 0.06961026306350959 1107 3 0.11890459022757938 1107 4 0.24879179975861376 1107 5 -0.061261905118804805 1107 6 -0.10474136101178999 1107 7 -0.15336113916387006 1107 8 -0.10739145361863223 1107 9 -0.13717258328120607 1107 10-11 0.029677647726664702 1107 12-13 0.028720893287164984 1107 14-15 -0.4083304885444221 1107 16-17 -0.1629725018844681 1107 18-19 0.004095335504558761 1107 20-21 -0.023483257016792436 1107 22-23 0.02210064465137407 1107 24-25 0.28424571150689104 1107 26-27 0.4885904377127517 1107 28-29 0.08687927015721897 1107 30-31 0.8739604959969469 1107 32-33 1.066827316890084 1107 34-35 -0.5949619316140584 1107 36-37 0.48511623807825544 1107 38-39 0.038935930288552356 1107 40-41 0.08205182067153771 1107 42-43 0.9139016405848821 1107 44-45 1.152468587532816 1107 46-47 1.7676883177019462 1107 48-49 1.6842789932445896 1107 50-51 1.8144533940524354 1107 52-53 1.7960982133577374 1107 54-55 2.4848013114149197 1107 56-57 1.5194221901334757 1107 58-59 1.7896062138127533 1107 60-61 1.541092730946609 1107 62-63 0.6070384302703999 1107 64-65 0.5487232835928069 1107 66-67 1.2838824515018565 1107 68-69 0.8176661268699235 1107 70-71 0.7080430255666954 1107 72-73 1.0069409839534913 1107 74-75 1.123364713392256 1107 76-77 0.922993804876004 1107 78-79 1.6499346445331433 1107 80-81 0.825631501374307 1107 82-83 1.065822842190709 1107 84-85 0.9270845774198442 1107 86-87 0.7662740246205324 1107 88-89 0.6801665903953982 1107 90-91 0.7672043477302779 1107 92-93 1.5842193115512124 1107 94-95 1.4616547134277411 1107 96-97 0.852503258510481 1107 98-99 1.6590014712607406 1107 100-101 0.9961233438802033 1108 1 -0.042331844646611216 1108 2 0.15931125974124427 1108 3 0.38658375491671393 1108 4 0.337543579540295 1108 5 -0.03753148271728435 1108 6 -0.016464189678139007 1108 7 0.4009815281356097 1108 8 0.43840826161629565 1108 9 0.5158886412085906 1108 10-11 0.1350407231894124 1108 12-13 0.26531320463030283 1108 14-15 0.45355845307874176 1108 16-17 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0.1989818178250431 2316 52-53 -0.6383333111900669 2316 54-55 -0.43121936041971054 2316 56-57 0.21354363716189795 2316 58-59 -0.3135914606058492 2316 60-61 0.12168058624376954 2316 62-63 0.20329166024456669 2316 64-65 -0.9078335639524155 2316 66-67 -0.25956070095292105 2316 68-69 -0.3588519609853762 2316 70-71 0.7020675733703108 2316 72-73 0.7685688909302364 2316 74-75 1.3727187185602148 2316 76-77 1.2548736498372435 2316 78-79 1.3579449804504584 2316 80-81 1.2991457132606108 2316 82-83 1.6943758137669391 2316 84-85 1.3464967221226836 2316 86-87 1.263851544000378 2316 88-89 1.0474146524109003 2316 90-91 0.7667198516062506 2316 92-93 2.10602163713261 2316 94-95 1.7001883051590099 2316 96-97 1.2234657908102236 2316 98-99 1.9132004376690084 2316 100-101 1.8268726978853707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 22.0 15 51.0 16 179.0 17 379.0 18 604.0 19 920.0 20 1252.0 21 1518.0 22 1479.0 23 1114.0 24 839.0 25 838.0 26 967.0 27 1144.0 28 1460.0 29 1262.0 30 762.0 31 696.0 32 796.0 33 952.0 34 1304.0 35 3143.0 36 3607.0 37 7974.0 38 12925.0 39 2888.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88616891064871 20.632394940840474 14.581803345573235 20.899632802937575 2 16.279211914997656 23.02724068376765 41.62099386728062 19.072553533954075 3 16.60112874635806 31.034412501782764 36.58034677370062 15.784111978158554 4 9.58008190542165 26.130274444285977 36.20138138994723 28.088262260345143 5 8.903649069904851 39.220879770175834 37.535910026283084 14.339561133636233 6 21.173162730995703 42.22000366740694 17.762474277215215 18.844359324382143 7 18.284061041951063 39.78729039750616 22.09408936248243 19.83455919806035 8 22.32432102035411 35.95688759397731 22.179662191071902 19.53912919459669 9 24.26600925001528 15.812636254355045 21.389132250769137 38.532222244860534 10-11 21.07740266090748 28.81155640675618 28.676066094822843 21.434974837513497 12-13 24.80797049774862 28.44685316110104 28.22986491717773 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15.317536317515943 30.056437317903058 36.3837330127748 18.2422933518062 40-41 15.278824799820704 29.9178908335201 37.1528697459302 17.650414620729 42-43 17.172632994437766 29.5755995191622 36.60683360159736 16.644933884802672 44-45 15.511093905992135 30.74203867076873 36.22379332124447 17.523074101994663 46-47 17.912226727246797 28.640410749577228 33.525193048226406 19.922169474949573 48-49 17.840916036755566 28.13104867463988 33.485462806381285 20.542572482223264 50-51 16.120290947617207 28.01593284570404 34.93408854750311 20.92968765917565 52-53 15.940995497239257 28.447871885250915 36.99293005439987 18.61820256310996 54-55 15.782074529858805 28.519182575742143 34.569385301847966 21.12935759255109 56-57 18.02326765958314 29.492064138872475 31.840223304333655 20.644444897210732 58-59 15.539618182188628 29.834355453230376 35.46076893298832 19.16525743159267 60-61 17.47213789450093 32.01646258226197 31.369572747091546 19.141826776145553 62-63 15.10666041849188 34.25969316028606 31.31965526374768 19.313991157474376 64-65 14.791874656180598 34.56836657769809 30.853079603105073 19.786679163016238 66-67 14.951814347710926 33.83590391393818 30.78788125751309 20.424400480837797 68-69 15.265581385872334 33.30514863185346 30.53116277174467 20.89810721052953 70-71 15.607872700230233 33.13298425052464 30.069680731851427 21.189462317393694 72-73 16.083616878221715 31.370591471241415 30.497544874798805 22.048246775738065 74-75 15.866628634298404 31.27992502190257 30.2398076648805 22.61363867891852 76-77 16.303661294594647 30.85104215480532 30.294818768973737 22.55047778162629 78-79 16.29551150139565 30.309080907071984 30.204152319634893 23.191255271897475 80-81 16.573623194311445 30.470039322752186 29.803793728734135 23.152543754202238 82-83 16.3016238462949 30.882622603451438 29.51243862186997 23.303314928383692 84-85 16.32811067419164 30.118579491045416 29.21497117010656 24.338338664656384 86-87 16.575660642611194 30.813349361259956 28.93380330474114 23.677186691387707 88-89 17.184857684236263 30.24693873392963 28.626148611478985 23.942054970355127 90-91 17.13697764919215 30.21332083698376 28.93991564964039 23.709785864183697 92-93 17.34479737576659 30.535237668344166 28.806462786006804 23.31350216988244 94-95 17.514924308795663 30.005501110409323 28.69847802612009 23.781096554674924 96-97 17.723762759519975 29.765082211038894 28.571137507385753 23.940017522055378 98-99 17.389621238361077 29.8333367290805 28.071962673947148 24.705079358611275 100-101 17.433426376805688 29.45131517287749 28.815631303355676 24.299627146961143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 962.0 1 584.0 2 215.5 3 241.0 4 187.5 5 129.0 6 186.5 7 331.5 8 483.0 9 484.0 10 308.5 11 180.0 12 179.5 13 174.5 14 166.0 15 168.0 16 181.5 17 180.0 18 167.0 19 188.0 20 204.5 21 222.5 22 230.5 23 246.5 24 282.5 25 347.0 26 444.0 27 562.0 28 675.5 29 782.0 30 902.5 31 1013.0 32 1166.0 33 1332.5 34 1572.0 35 1739.0 36 1734.0 37 1788.0 38 1810.0 39 1781.5 40 1759.5 41 1676.0 42 1541.5 43 1511.0 44 1484.5 45 1365.0 46 1315.0 47 1356.5 48 1681.5 49 1796.5 50 1768.5 51 1689.5 52 1219.5 53 887.5 54 728.0 55 591.0 56 475.0 57 356.5 58 321.5 59 293.5 60 217.5 61 162.0 62 127.0 63 96.0 64 75.0 65 65.5 66 61.5 67 54.5 68 39.5 69 39.0 70 38.0 71 35.5 72 38.5 73 47.0 74 44.0 75 28.0 76 17.0 77 6.5 78 4.0 79 4.5 80 2.0 81 2.5 82 3.0 83 1.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12428434628471302 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 49081.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.531244269676655 #Duplication Level Percentage of deduplicated Percentage of total 1 75.063490673439 34.92797620260386 2 11.813643926788686 10.99407102544773 3 4.816533847096944 6.723579389173 4 2.4476749277519922 4.555734398239645 5 1.5193974954023997 3.5349728000651983 6 0.9939574393554602 2.7750045842586744 7 0.6392854015237761 2.0822721623438807 8 0.5648480602504597 2.1026466453413746 9 0.2977493650932656 1.246918359446629 >10 1.7120588492862774 12.998920152401134 >50 0.06130133987214292 1.8601902976711964 >100 0.04378667133724494 4.578146329536888 >500 0.02189333566862247 7.888999816629654 >1k 0.004378667133724494 3.73056783684114 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1831 3.73056783684114 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 997 2.0313359548501455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 847 1.7257187098877365 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 839 1.7094191234897416 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 682 1.3895397404290866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 507 1.0329862879729428 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 482 0.9820500804792078 RNA PCR Primer, Index 46 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 354 0.7212566981112855 RNA PCR Primer, Index 46 (95% over 23bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 322 0.6560583525193048 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 257 0.5236242130355943 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.4013773150506306 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.3545160041563945 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 131 0.2669057272671706 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 122 0.24856869256942604 RNA PCR Primer, Index 46 (96% over 26bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.21189462317393695 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.21189462317393695 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 94 0.191520140176443 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 84 0.17114565717894908 TruSeq Adapter, Index 4 (95% over 23bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.16299586397995153 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.14873372588170575 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 73 0.14873372588170575 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66 0.13447158778346 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 61 0.12428434628471302 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 59 0.12020944968521424 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC 58 0.11817200138546484 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 55 0.11205965648621666 RNA PCR Primer, Index 46 (96% over 28bp) CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 55 0.11205965648621666 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 53 0.10798475988671788 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 51 0.10390986328721909 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.10390986328721909 No Hit GTTCAGGAGTGATCTGGGCACAGAAGCCGAAGTCAGTAAGTTTAACTGAG 50 0.1018724149874697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.001018724149874697 0.0 16-17 0.0 0.0 0.0 0.002037448299749394 0.0 18-19 0.0 0.0 0.0 0.002037448299749394 0.0 20-21 0.0 0.0 0.0 0.002037448299749394 0.0 22-23 0.0 0.0 0.0 0.014262138098245756 0.0 24-25 0.0 0.0 0.0 0.04074896599498788 0.0 26-27 0.0 0.0 0.0 0.0977975183879709 0.0 28-29 0.0 0.0 0.0 0.20476355412481406 0.0 30-31 0.0 0.0 0.0 0.38304028035288606 0.0 32-33 0.0 0.0 0.0 0.5684480756300809 0.0 34-35 0.0 0.0 0.0 0.8424848719463743 0.0 36-37 0.0 0.0 0.0 1.1307838063609137 0.0 38-39 0.0 0.0 0.0 1.4292699822741999 0.0 40-41 0.0 0.0 0.0 1.8744524357694423 0.0 42-43 0.0 0.0 0.0 2.194331818830097 0.0 44-45 0.0 0.0 0.0 2.4724435117458894 0.0 46-47 0.0 0.0 0.0 2.752592652961431 0.0 48-49 0.0 0.0 0.0 3.044966483975469 0.0 50-51 0.0 0.0 0.0 3.3098347629428906 0.0 52-53 0.0 0.0 0.0 3.5573847313624416 0.0 54-55 0.0 0.0 0.0 3.8181781137303643 0.0 56-57 0.0 0.0 0.0 4.219555428780994 0.0 58-59 0.0 0.0 0.0 4.514985432244657 0.0 60-61 0.0 0.0 0.0 4.805321814958946 0.0 62-63 0.0 0.0 0.0 5.105845439171981 0.0 64-65 0.0 0.0 0.0 5.372751166439151 0.0 66-67 0.0 0.0 0.0 5.683462032150935 0.0 68-69 0.0 0.0 0.0 6.1021576577494345 0.0 70-71 0.0 0.0 0.0 6.596238870438663 0.0 72-73 0.0 0.0 0.0 7.021046840936411 0.0 74-75 0.0 0.0 0.0 7.468266742731403 0.0 76-77 0.0 0.0 0.0 7.896130885678776 0.0 78-79 0.0 0.0 0.0 8.37187506367026 0.0 80-81 0.0 0.0 0.0 8.903649069904851 0.0 82-83 0.0 0.0 0.0 9.420142213891323 0.0 84-85 0.0 0.0 0.0 9.915242150730425 0.0 86-87 0.0 0.0 0.0 10.40219229437053 0.0 88-89 0.0 0.0 0.0 10.916647990057253 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATAGCT 20 0.0020623982 71.248474 9 GTTTAGA 25 0.004952931 57.115223 1 TTATAGC 25 0.004993163 56.998775 8 GAAAGCG 35 2.76194E-4 54.284546 9 ACCCATG 35 2.76194E-4 54.284546 3 TAAAGAA 40 5.3381466E-4 47.49898 5 TACCCAT 45 9.5360976E-4 42.221313 2 TTGGAGT 45 9.5360976E-4 42.221313 4 GTACCCA 50 0.0015849419 38.076817 1 TACGAAT 25 0.0016087892 37.999184 34-35 CGCAGAA 105 4.504909E-8 36.1897 9 GGTATCA 775 0.0 35.620247 1 AGTATGA 30 0.0039339266 31.665987 70-71 TATGAGT 30 0.0039339266 31.665987 72-73 GCAGAGA 65 0.0057910103 29.23014 9 GTATCAA 1105 0.0 28.42839 1 TTAGTTC 35 0.008355739 27.142273 66-67 CGCAGAG 1180 0.0 26.164692 8 AACGCAG 1200 0.0 26.124441 6 TCAACGC 1245 0.0 25.180182 4 >>END_MODULE