FastQCFastQC Report
Fri 10 Feb 2017
SRR2031846_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031846_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30341
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5361.7665864671566527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4791.5787218615075311No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.9623941201674303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.8635180119310504No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.5767772980455489No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.3856168221218813No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT930.3065159355327774No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.28674071388550143No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.2702613625127715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.26366962196367955No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG720.23730265976731157No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT680.2241191786691276No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA680.2241191786691276No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT620.20434395702185162No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA590.19445634619821364No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.19445634619821364No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.18786460564912166No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA520.17138525427639167No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG430.14172242180547773No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT430.14172242180547773No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT430.14172242180547773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.13183481098183977No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT400.13183481098183977No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.13183481098183977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC330.1087637190600178No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA330.1087637190600178No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT320.1054678487854718No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.10217197851092581No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC310.10217197851092581No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC310.10217197851092581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGG200.002054581471.252
GTATATG200.002054581471.251
GTACCCA301.2809715E-463.3333361
TACCCAT301.2809715E-463.3333362
ACCCATG405.305046E-447.5000043
GGTATCA7250.045.2068981
CGCAGAA1350.042.222229
GTACATA308.1980834E-539.58333614-15
CATAAGC308.1980834E-539.58333616-17
TACATAA352.0305483E-433.92857414-15
AACGCAG9850.033.2741136
CGCAGAG9250.032.3513538
GTATCAA10900.032.2477071
ACGCAGA10300.031.8203897
CAACGCA10850.031.0829475
TCAACGC10900.030.9403674
GCAGAGT8850.030.5932229
TATCAAC11800.030.1906782
ATCAACG11800.030.1906783
ATAAGCA404.4441444E-429.68750218-19
Produced by FastQC (version 0.11.5)