##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031846_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30341 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2939916284895 33.0 31.0 34.0 30.0 34.0 2 32.418905111894794 34.0 31.0 34.0 31.0 34.0 3 32.32929039912989 34.0 31.0 34.0 30.0 34.0 4 35.95418740318381 37.0 35.0 37.0 35.0 37.0 5 35.982465970139415 37.0 35.0 37.0 35.0 37.0 6 36.11515770739264 37.0 36.0 37.0 35.0 37.0 7 36.029201410632474 37.0 35.0 37.0 35.0 37.0 8 36.10052404337365 37.0 36.0 37.0 35.0 37.0 9 37.77789130219835 39.0 38.0 39.0 35.0 39.0 10-11 37.75976401568835 39.0 38.0 39.0 35.0 39.0 12-13 37.73326521868099 39.0 38.0 39.0 35.0 39.0 14-15 38.93339046175143 41.0 38.0 41.0 35.5 41.0 16-17 38.81612339738308 40.0 38.0 41.0 35.0 41.0 18-19 38.99850037902508 41.0 38.5 41.0 35.0 41.0 20-21 39.125770409676676 41.0 38.5 41.0 35.0 41.0 22-23 38.956576909132856 41.0 38.0 41.0 35.0 41.0 24-25 38.705513990969315 40.0 38.0 41.0 35.0 41.0 26-27 38.289459806862 40.0 36.5 41.0 35.0 41.0 28-29 37.84997198510267 40.0 35.0 41.0 33.5 41.0 30-31 37.04968524438878 38.5 35.0 41.0 33.0 41.0 32-33 35.62427078870176 37.0 35.0 40.0 30.0 41.0 34-35 35.040753435944765 37.0 35.0 40.0 27.0 41.0 36-37 34.57898553112949 37.0 35.0 40.0 23.5 41.0 38-39 33.36951649583072 35.0 33.0 40.0 17.0 41.0 40-41 33.12872021357239 35.0 33.0 40.0 14.5 41.0 42-43 32.35120793645562 35.0 32.0 40.0 9.0 41.0 44-45 31.230002307109192 35.0 29.5 40.0 7.0 41.0 46-47 30.227464486997793 35.0 24.0 39.5 7.0 41.0 48-49 29.611944233874954 35.0 21.0 39.0 7.0 41.0 50-51 28.340018456873537 35.0 16.0 37.5 7.0 41.0 52-53 28.352542763916812 35.0 15.0 37.0 7.0 41.0 54-55 28.14697933489338 35.0 13.0 37.0 7.0 41.0 56-57 27.37068652977819 34.0 10.0 36.0 7.0 41.0 58-59 26.983026268086086 34.0 10.0 36.0 7.0 40.5 60-61 25.946952967931182 32.5 10.0 36.0 7.0 40.0 62-63 24.732490689166475 31.0 9.0 35.0 7.0 39.5 64-65 23.499901123891764 27.5 8.0 35.0 7.0 39.0 66-67 22.082726343891103 22.5 7.0 35.0 7.0 37.5 68-69 20.28962460037573 18.5 7.0 35.0 6.0 37.0 70-71 18.854800435054877 15.0 7.0 34.0 5.0 36.0 72-73 17.854783955703503 10.0 7.0 33.0 5.0 35.0 74-75 17.733331136086484 10.0 7.0 33.0 5.0 35.0 76-77 17.304011074124123 10.0 7.0 30.5 5.0 35.0 78-79 17.813255990244222 10.0 7.0 32.5 5.0 35.0 80-81 17.823473188095313 10.0 7.0 33.0 5.0 35.0 82-83 17.755265152763585 10.0 7.0 32.0 5.0 35.0 84-85 17.574321874691012 10.0 7.0 32.0 5.0 35.0 86-87 17.48170791997627 10.0 7.0 32.0 5.0 35.0 88-89 17.3527899541874 10.0 7.0 31.0 5.0 35.0 90-91 17.198164200257075 10.0 7.0 31.0 5.0 35.0 92-93 17.082858178702086 10.0 7.0 31.0 7.0 35.0 94-95 17.051827560067238 10.0 7.0 31.0 7.0 35.0 96-97 16.974209815101677 10.0 7.0 31.0 7.0 35.0 98-99 16.850515803697967 10.0 7.0 30.5 7.0 35.0 100-101 16.11947529745229 10.0 7.0 27.5 7.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25780526567822193 1101 2 -0.021778177767885154 1101 3 -0.5252401742745931 1101 4 -0.3624930633037522 1101 5 0.08301345665829984 1101 6 -0.11280868102513608 1101 7 0.1481989769833305 1101 8 -0.07315950612301947 1101 9 -0.32503365310829224 1101 10-11 -0.1388831494872491 1101 12-13 0.20289565296868517 1101 14-15 0.29958115002740016 1101 16-17 -0.6694431996422239 1101 18-19 0.5004310203515772 1101 20-21 0.8013061515272639 1101 22-23 1.2947677646367879 1101 24-25 0.9881023479096029 1101 26-27 0.9456191696617182 1101 28-29 0.5471395022905128 1101 30-31 0.3772135701913939 1101 32-33 -0.44187277710716444 1101 34-35 -0.041647709713465986 1101 36-37 -0.1753553026633199 1101 38-39 0.40714108184555897 1101 40-41 0.31858327620792437 1101 42-43 0.2306507379867071 1101 44-45 -0.7079826109917455 1101 46-47 -2.131476120038837 1101 48-49 -1.5419499935386831 1101 50-51 -1.6191877638321905 1101 52-53 -1.5546798171523903 1101 54-55 -1.351590231088938 1101 56-57 -0.4278237314058835 1101 58-59 -0.9270993489036741 1101 60-61 -0.3067591971534185 1101 62-63 0.5369512405249957 1101 64-65 0.452473601026135 1101 66-67 0.4152925354030792 1101 68-69 -0.781248859549148 1101 70-71 0.6168926394683787 1101 72-73 0.8658249285471449 1101 74-75 -0.5937992040701445 1101 76-77 -0.8683741034815817 1101 78-79 -0.1800189558598646 1101 80-81 0.7935772235902689 1101 82-83 -1.6949190952210138 1101 84-85 -2.0007145302426164 1101 86-87 -1.71692435912305 1101 88-89 -0.8094751665296052 1101 90-91 -2.1882381612551995 1101 92-93 -2.9399995543572572 1101 94-95 -1.0064799855244289 1101 96-97 -1.7543101027903667 1101 98-99 -3.5782445836487167 1101 100-101 -2.8665367508496864 1102 1 0.6409221487951058 1102 2 0.5496503936606842 1102 3 0.5743269252925032 1102 4 -0.024830725641415086 1102 5 -0.010060136415297904 1102 6 0.4781004098839574 1102 7 0.5464673752517228 1102 8 0.4246759917124763 1102 9 0.7896849616103268 1102 10-11 -0.05771431831841056 1102 12-13 0.6119865620595917 1102 14-15 0.8536937041399497 1102 16-17 0.7299074497084206 1102 18-19 0.9290024489230078 1102 20-21 0.9149425151636237 1102 22-23 0.9397894096584309 1102 24-25 1.0205698803771313 1102 26-27 0.4293854034279505 1102 28-29 0.38696634211735415 1102 30-31 1.1683391113169392 1102 32-33 0.7464389112045282 1102 34-35 1.4064042383384887 1102 36-37 2.3657702384622254 1102 38-39 2.5456692203737035 1102 40-41 2.901916609541253 1102 42-43 2.7328152401512114 1102 44-45 0.8017576487485165 1102 46-47 0.6672251786624592 1102 48-49 1.6138941623054741 1102 50-51 1.711981067336641 1102 52-53 1.5015972391246706 1102 54-55 2.072652193153491 1102 56-57 2.1317000781179267 1102 58-59 1.6107794389751149 1102 60-61 1.3360979457037239 1102 62-63 0.8226655262392804 1102 64-65 1.1104822590347894 1102 66-67 1.3871540072645523 1102 68-69 1.2425606642603775 1102 70-71 1.2489272715030104 1102 72-73 1.9881193008415146 1102 74-75 2.780659670388733 1102 76-77 1.92275143764396 1102 78-79 2.0829680571271467 1102 80-81 0.8779928080058532 1102 82-83 5.0680679177659975 1102 84-85 3.1280733485452643 1102 86-87 2.629395987197295 1102 88-89 3.0693127122582773 1102 90-91 1.6732337002166595 1102 92-93 2.3435502291925268 1102 94-95 3.116896637852193 1102 96-97 1.7705816721014074 1102 98-99 1.0527077973036647 1102 100-101 -0.2550648893778238 1103 1 0.39471002758298823 1103 2 0.6746503936606842 1103 3 0.9152360162015967 1103 4 0.6494116986010141 1103 5 0.7020610757059131 1103 6 0.6371913189748639 1103 7 0.576770405554754 1103 8 0.5042214462579295 1103 9 0.28210920403456896 1103 10-11 0.6468311362270427 1103 12-13 0.5778956529686852 1103 14-15 0.8233906738369186 1103 16-17 0.8871044194053894 1103 18-19 1.1373357822563364 1103 20-21 1.0096394848605925 1103 22-23 1.089410621779649 1103 24-25 0.8898880621953182 1103 26-27 0.775976312518857 1103 28-29 1.16916331181433 1103 30-31 1.219475474953299 1103 32-33 1.4718176990833172 1103 34-35 2.398828480762724 1103 36-37 3.062739935431921 1103 38-39 3.4964267961312814 1103 40-41 2.4435832762079173 1103 42-43 3.0699364522724224 1103 44-45 1.860469769960634 1103 46-47 -0.5392142152769317 1103 48-49 1.5116214350327475 1103 50-51 2.770693188548762 1103 52-53 2.9334154209428505 1103 54-55 4.231743102244398 1103 56-57 1.8608667447845946 1103 58-59 2.489567317762994 1103 60-61 2.586097945703724 1103 62-63 1.5518321929059447 1103 64-65 0.5953307438832738 1103 66-67 0.2962449163554588 1103 68-69 0.8258939975937096 1103 70-71 1.5216545442302838 1103 72-73 1.722967785689999 1103 74-75 1.9567960340250963 1103 76-77 1.0363878012803234 1103 78-79 1.54319532985442 1103 80-81 3.4632200807331266 1103 82-83 4.153295190493271 1103 84-85 1.9992854697573819 1103 86-87 2.006289926591233 1103 88-89 2.6488581668037305 1103 90-91 1.7849761244590852 1103 92-93 1.6522623504046443 1103 94-95 2.5203057287612864 1103 96-97 2.522475611495345 1103 98-99 1.9902077973036647 1103 100-101 1.5858442015312662 1104 1 0.3263766942496531 1104 2 0.1896503936606848 1104 3 -0.17476398379840674 1104 4 0.10607836526767755 1104 5 0.47539440903924657 1104 6 0.5671913189748636 1104 7 0.35010373888808743 1104 8 0.4392214462579318 1104 9 0.8387758707012338 1104 10-11 0.6268311362270467 1104 12-13 0.5828956529686806 1104 14-15 1.0900573405035843 1104 16-17 0.3562710860720557 1104 18-19 1.1690024489230098 1104 20-21 0.5413061515272659 1104 22-23 0.7052439551129766 1104 24-25 0.46238806219532336 1104 26-27 0.6284763125188633 1104 28-29 0.5033299784809984 1104 30-31 1.6019754749532993 1104 32-33 -0.5390156342500134 1104 34-35 -0.5045048525706051 1104 36-37 -0.13059339790141422 1104 38-39 -1.805239870535388 1104 40-41 -3.0480833904587463 1104 42-43 -2.781730214394244 1104 44-45 -2.2536968967060282 1104 46-47 -1.7600475486102667 1104 48-49 -1.6533785649672517 1104 50-51 -1.873473478117905 1104 52-53 -0.8165845790571495 1104 54-55 -2.438256897755604 1104 56-57 -2.3782999218820713 1104 58-59 -1.6337660155703375 1104 60-61 -0.9639020542962733 1104 62-63 -0.9973344737607199 1104 64-65 -0.6113359227833897 1104 66-67 -0.5237550836445415 1104 68-69 0.30256066426037975 1104 70-71 -1.2783454557697134 1104 72-73 -0.1836988809766673 1104 74-75 0.20429603402509855 1104 76-77 -1.1436121987196763 1104 78-79 -1.2343046701455798 1104 80-81 -1.369279919266873 1104 82-83 -0.389204809506726 1104 84-85 -0.4073811969092809 1104 86-87 -0.9778767400754305 1104 88-89 -0.8961418331962712 1104 90-91 0.08414279112575329 1104 92-93 -1.9619043162620198 1104 94-95 -2.5921942712387143 1104 96-97 -2.00669105517132 1104 98-99 0.4027077973036661 1104 100-101 -0.574155798468734 1105 1 0.32923383710679843 1105 2 -0.34320674919646166 1105 3 0.09380744477302727 1105 4 0.4589355081248243 1105 5 0.42825155189639474 1105 6 0.6014770332605792 1105 7 0.2672465960309438 1105 8 0.23636430340078363 1105 9 0.020204442129802658 1105 10-11 -0.138883149487242 1105 12-13 -0.40424720417417603 1105 14-15 -0.5635140880678406 1105 16-17 0.16984251464349143 1105 18-19 0.41114530606586897 1105 20-21 -0.7701224199013055 1105 22-23 -0.5861846163155917 1105 24-25 -0.33332622351896646 1105 26-27 0.5170477410902876 1105 28-29 0.576901407052425 1105 30-31 0.8712611892390072 1105 32-33 0.7545557943214121 1105 34-35 -0.005933423999174181 1105 36-37 -0.3598791121871301 1105 38-39 -1.17023987053539 1105 40-41 -0.6516548190301776 1105 42-43 -0.900301642965669 1105 44-45 -1.1722683252774608 1105 46-47 -2.220761834324552 1105 48-49 -2.434807136395822 1105 50-51 -2.6191877638321905 1105 52-53 -2.3522988647714342 1105 54-55 -2.7325426120413177 1105 56-57 -3.225442779024931 1105 58-59 -1.4866231584274807 1105 60-61 -1.1103306257248455 1105 62-63 -1.8380487594750043 1105 64-65 -1.01776449421196 1105 66-67 0.3855306306411741 1105 68-69 -1.6502964785967684 1105 70-71 -2.353345455769716 1105 72-73 -2.550841738119523 1105 74-75 -2.569989680260619 1105 76-77 -2.535040770148248 1105 78-79 -2.072876098717007 1105 80-81 -2.7421370621240158 1105 82-83 -3.8913476666495868 1105 84-85 -2.52452405405214 1105 86-87 -2.865733882932574 1105 88-89 -3.958284690339127 1105 90-91 -0.9501429231599623 1105 92-93 -0.9340471734048776 1105 94-95 -0.36362284266728295 1105 96-97 -0.992405340885604 1105 98-99 -0.21514934555347764 1105 100-101 -1.9141557984687338 1106 1 0.4794801425255173 1106 2 0.13585729021240667 1106 3 -0.0933846734535777 1106 4 0.3419404342331944 1106 5 0.5526357883495905 1106 6 0.2665016638024511 1106 7 -0.12437901973260068 1106 8 0.3274973083268975 1106 9 0.7160172500115749 1106 10-11 0.5433828603649715 1106 12-13 0.013240480554891576 1106 14-15 -0.3720116250136485 1106 16-17 -0.9871771897900032 1106 18-19 -0.8123768614218179 1106 20-21 -0.7331766070934265 1106 22-23 -0.48027328626633903 1106 24-25 -0.5389912481495074 1106 26-27 -0.5666961012742462 1106 28-29 -0.13184243531210882 1106 30-31 0.38542375081537017 1106 32-33 0.9768464347154975 1106 34-35 1.2120468715673312 1106 36-37 1.074234188305482 1106 38-39 2.851311853602546 1106 40-41 2.7898476440240145 1106 42-43 3.9334421993988613 1106 44-45 2.109751379156041 1106 46-47 3.146849003113875 1106 48-49 4.265931779860335 1106 50-51 3.8044575563648557 1106 52-53 3.7351395588738825 1106 54-55 4.016225860865088 1106 56-57 4.2265276643248235 1106 58-59 1.492440880981384 1106 60-61 1.5602358767382078 1106 62-63 1.4605965607220384 1106 64-65 0.5838364910097127 1106 66-67 1.7617621577347684 1106 68-69 2.914974457363826 1106 70-71 1.8837235097475258 1106 72-73 2.3838873259198863 1106 74-75 1.4029167236802706 1106 76-77 1.2432843530044622 1106 78-79 0.7263849850268329 1106 80-81 1.3576166324572654 1106 82-83 2.4528641560105093 1106 84-85 1.7464119065389951 1106 86-87 0.04005429440732655 1106 88-89 -0.21752114354109509 1106 90-91 0.19517727388437223 1106 92-93 0.8153370630483217 1106 94-95 2.6043574528992153 1106 96-97 1.7415848068976487 1106 98-99 1.6647767628209067 1106 100-101 0.6030855808416113 1107 1 0.35304336091631683 1107 2 -0.017016273005985738 1107 3 0.1985693495349281 1107 4 0.466078365267677 1107 5 0.5020610757059174 1107 6 0.6871913189748611 1107 7 0.576770405554754 1107 8 0.6792214462579267 1107 9 0.16544253736790182 1107 10-11 0.6134978028937113 1107 12-13 0.6862289863020195 1107 14-15 1.0067240071702557 1107 16-17 0.46627108607205514 1107 18-19 0.6123357822563449 1107 20-21 0.8846394848605925 1107 22-23 0.6519106217796491 1107 24-25 1.0523880621953126 1107 26-27 0.8218096458521913 1107 28-29 -0.010003354852337054 1107 30-31 0.14864214161996614 1107 32-33 -0.7823489675833528 1107 34-35 -0.5345048525706062 1107 36-37 -0.25392673123474907 1107 38-39 0.4047601294646128 1107 40-41 0.38524994287458725 1107 42-43 -1.7050635477275762 1107 44-45 -0.19369689670603307 1107 46-47 -0.34338088194359884 1107 48-49 -1.5633785649672554 1107 50-51 -1.7334734781179044 1107 52-53 -2.3999179123904852 1107 54-55 -1.318256897755603 1107 56-57 -0.884966588548739 1107 58-59 -1.393766015570339 1107 60-61 -1.3305687209629404 1107 62-63 -0.9273344737607196 1107 64-65 -2.7046692561167234 1107 66-67 -2.8704217503112055 1107 68-69 -1.7574393357396225 1107 70-71 -2.5783454557697176 1107 72-73 -1.8936988809766646 1107 74-75 -3.4057039659749044 1107 76-77 -3.6802788653863434 1107 78-79 -3.64430467014558 1107 80-81 -2.899279919266874 1107 82-83 -2.7425381428400613 1107 84-85 -3.0507145302426153 1107 86-87 -3.997876740075432 1107 88-89 -2.076141833196271 1107 90-91 -1.0358572088742477 1107 92-93 -1.2685709829286864 1107 94-95 -4.125527604572046 1107 96-97 -4.106691055171318 1107 98-99 -3.0972922026963357 1107 100-101 -2.2974891318020667 1108 1 0.7898249701117237 1108 2 0.6530986695227554 1108 3 0.9066153265464223 1108 4 0.7212507790607816 1108 5 -0.137019384064196 1108 6 0.12857062931968954 1108 7 0.6342416699225737 1108 8 0.3274973083268975 1108 9 0.4746379396667493 1108 10-11 0.5261414810546299 1108 12-13 0.27186117021006595 1108 14-15 0.9383332025725579 1108 16-17 0.943857292968616 1108 18-19 0.39451969030231737 1108 20-21 -0.06076281398997452 1108 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2312 96-97 -0.5924053408856054 2312 98-99 0.0884220830179494 2312 100-101 -1.5712986556115904 2313 1 -0.7302899724170153 2313 2 -0.7559051618948729 2313 3 -1.0292084282428462 2313 4 -0.8505883013989859 2313 5 -0.3257167020718654 2313 6 -0.22391979213625035 2313 7 -0.11767403888968886 2313 8 -0.12077855374207047 2313 9 -0.30122412929876674 2313 10-11 -1.3809466415507359 2313 12-13 -1.352659902586872 2313 14-15 -0.8571648817186315 2313 16-17 -0.28650669170571774 2313 18-19 -0.29321977329921367 2313 20-21 -1.0320271818060647 2313 22-23 -3.861978267109251 2313 24-25 -2.6865008266935675 2313 26-27 -1.7587459097033644 2313 28-29 -1.448892243741227 2313 30-31 -0.5235800806022581 2313 32-33 -0.6184600786944614 2313 34-35 -2.406727074792826 2313 36-37 -3.326148953456972 2313 38-39 -3.5869065372020543 2313 40-41 -3.848083390458747 2313 42-43 -2.402285769949799 2313 44-45 -2.410363563372698 2313 46-47 -2.837825326388046 2313 48-49 -3.585600787189474 2313 50-51 -2.5556957003401273 2313 52-53 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10-11 -0.9557329663370595 2314 12-13 -0.7073607572877236 2314 14-15 -0.44584009539384795 2314 16-17 -0.793985324184348 2314 18-19 -0.04535652543597024 2314 20-21 0.07053692075803752 2314 22-23 -0.3224483525793289 2314 24-25 -0.37453501472776196 2314 26-27 -0.6807544567119166 2314 28-29 -0.8997469445959254 2314 30-31 -1.743665550687723 2314 32-33 -0.6579899932243762 2314 34-35 0.09754642948067982 2314 36-37 -0.860336987645006 2314 38-39 -0.8272911525866746 2314 40-41 -2.3737244160997726 2314 42-43 -2.9781404708044974 2314 44-45 -2.4936968967060302 2314 46-47 -1.0600475486102674 2314 48-49 -0.12619907778776351 2314 50-51 -0.16680681145124154 2314 52-53 -0.36786663033920064 2314 54-55 0.03302515352644875 2314 56-57 0.9329821293999778 2314 58-59 0.8934134716091506 2314 60-61 1.2848158944216728 2314 62-63 -0.8952831917094386 2314 64-65 -0.9303102817577482 2314 66-67 -1.4729858528753077 2314 68-69 -1.4369265152268014 2314 70-71 -1.362960840385103 2314 72-73 -0.14882708610486972 2314 74-75 -0.2531398634108015 2314 76-77 -0.5918173269248079 2314 78-79 0.8877466119057047 2314 80-81 0.855848285861331 2314 82-83 -0.0630509633528824 2314 84-85 0.5890290595009731 2314 86-87 0.658533516334824 2314 88-89 0.33155047449603714 2314 90-91 0.03337356035652306 2314 92-93 -1.253186367544071 2314 94-95 -1.1896301686746114 2314 96-97 -1.8989987474790109 2314 98-99 -1.6972922026963353 2314 100-101 -1.6449250292379638 2315 1 -0.1191788613059046 2315 2 -0.061460717450430025 2315 3 0.08190268286826097 2315 4 0.010522809712121273 2315 5 -1.3812722576274155 2315 6 -1.0850309032473575 2315 7 -0.20100737222302456 2315 8 -0.06522299818651334 2315 9 0.6987758707012333 2315 10-11 -0.4642799748840716 2315 12-13 -1.2971043470313148 2315 14-15 -1.2877204372741886 2315 16-17 -0.008728913927939175 2315 18-19 -0.9043308844103208 2315 20-21 -0.8514716262505146 2315 22-23 -0.36197826710924375 2315 24-25 0.20238806219531824 2315 26-27 -0.48096813192558585 2315 28-29 0.009441089592108654 2315 30-31 -0.07913563615781527 2315 32-33 -0.7990156342500185 2315 34-35 -2.2122826303483833 2315 36-37 -1.006704509012529 2315 38-39 -0.12857320386872573 2315 40-41 -0.05641672379207563 2315 42-43 0.7504920078279866 2315 44-45 -2.007585785594916 2315 46-47 -1.4350475486102674 2315 48-49 -2.099489676078367 2315 50-51 -3.333473478117906 2315 52-53 -3.4276956901682603 2315 54-55 -1.0182568977556024 2315 56-57 -1.9932999218820733 2315 58-59 -0.5243215711258955 2315 60-61 -0.33056872096294043 2315 62-63 -1.2328900293162768 2315 64-65 -1.349113700561169 2315 66-67 -1.1343106392000983 2315 68-69 0.03422733092704533 2315 70-71 1.4660989886747267 2315 72-73 0.945190007912224 2315 74-75 1.8665182562473177 2315 76-77 1.4808322457247662 2315 78-79 1.1473619965210844 2315 80-81 0.9146089696220123 2315 82-83 1.0630174127154923 2315 84-85 -0.028492308020393153 2315 86-87 1.7076788154801257 2315 88-89 1.8571915001370662 2315 90-91 1.0835872355701959 2315 92-93 2.0758734615157586 2315 94-95 2.846694617650172 2315 96-97 3.1266422781620165 2315 98-99 3.038818908414779 2315 100-101 4.183066423753488 2316 1 0.1085989164718768 2316 2 -0.09479405078376146 2316 3 -0.045875094909511915 2316 4 0.3549672541565698 2316 5 0.4576166312614731 2316 6 -0.15725312546958037 2316 7 -0.3343407055563574 2316 8 -0.20966744263095904 2316 9 -1.7345574626320968 2316 10-11 -0.5976133082173973 2316 12-13 -1.1526599025868691 2316 14-15 -0.9210537706075215 2316 16-17 -0.7726178028168249 2316 18-19 -0.3932197732992151 2316 20-21 -0.1098049595838475 2316 22-23 0.19635506622408627 2316 24-25 -0.4309452711380146 2316 26-27 0.22180964585218987 2316 28-29 -0.15444779929678276 2316 30-31 0.03753103050885187 2316 32-33 0.11209547686109289 2316 34-35 -0.7845048525705991 2316 36-37 -0.3928156201236348 2316 38-39 0.7769823516868328 2316 40-41 0.24080549843014154 2316 42-43 -0.3661746588386876 2316 44-45 0.8063031032939669 2316 46-47 0.7843968958341776 2316 48-49 0.6088436572549689 2316 50-51 0.6887487441043163 2316 52-53 -0.349917912390481 2316 54-55 1.015076435577729 2316 56-57 1.0705889670068167 2316 58-59 1.6729006510963274 2316 60-61 0.5583201679259453 2316 62-63 0.4115544151281689 2316 64-65 0.3397751883277209 2316 66-67 1.5518004719110117 2316 68-69 -0.16855044685073395 2316 70-71 -0.44501212243638477 2316 72-73 -0.8325877698655546 2316 74-75 -0.8779261881971259 2316 76-77 -1.508056643164121 2316 78-79 -0.2943046701455785 2316 80-81 0.6062756362886823 2316 82-83 0.13523963493771518 2316 84-85 -0.7118256413537267 2316 86-87 -0.14232118451987574 2316 88-89 -0.30947516652960516 2316 90-91 -0.7803016533186913 2316 92-93 -0.2685709829286864 2316 94-95 -0.48108316012760355 2316 96-97 -0.5511354996157607 2316 98-99 -1.0195144249185581 2316 100-101 -0.6363780206909553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 9.0 14 27.0 15 102.0 16 305.0 17 679.0 18 967.0 19 1288.0 20 1638.0 21 1820.0 22 1618.0 23 1114.0 24 1048.0 25 1413.0 26 1975.0 27 2450.0 28 2527.0 29 1945.0 30 718.0 31 372.0 32 419.0 33 519.0 34 528.0 35 954.0 36 1332.0 37 2692.0 38 1773.0 39 108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.0938489968321 22.74947201689546 12.496700105596622 25.659978880675823 2 13.84595102336772 20.978214297485252 44.952374674532805 20.223460004614218 3 14.630368148709666 35.905210770904056 40.33815629016842 9.126264790217856 4 6.8224514683102075 31.56784548960153 23.819254474143897 37.79044856794437 5 5.247025477077223 38.350746514617185 39.96242707887018 16.439800929435417 6 13.691045120464059 38.950594904584555 17.774628390626546 29.58373158432484 7 9.937048877756173 38.78580139085726 16.22556936158993 35.05158036979664 8 20.36188655614515 34.27045911472925 26.729507926568008 18.638146402557595 9 19.27424936554497 13.137338914340333 14.119508256155038 53.46890346395966 10-11 19.874097755512345 27.495797765399953 25.393032530239612 27.23707194884809 12-13 25.514979730397812 25.393032530239612 24.798127945684058 24.29385979367852 14-15 20.068554101710557 26.131307471737912 22.382255034441844 31.417883392109687 16-17 17.896575590784746 36.99120002636696 22.186150753106357 22.926073629741932 18-19 20.40473286971425 26.182393460993374 29.502982762598464 23.909890906693914 20-21 17.67245641211562 25.473781351965986 29.85728881711216 26.99647341880624 22-23 14.745723608318775 36.91209913977786 29.11901387561386 19.22316337628951 24-25 20.511848653636992 36.90221152895422 30.224778352724037 12.36116146468475 26-27 21.284730233018028 35.98101578721862 31.440954484031508 11.293299495731848 28-29 15.432912560561615 26.742691407666197 37.79868824363073 20.02570778814146 30-31 15.512013447150721 38.952242839721826 31.74417454928974 13.79156916383771 32-33 25.032134735176825 27.495797765399953 36.42925414455687 11.042813354866352 34-35 15.6718631554662 24.789888269997693 38.10190830888896 21.436340265647143 36-37 11.73988991793283 37.18565637256518 33.522296562407305 17.55215714709469 38-39 13.53284334728585 24.311987080188523 39.54055568372829 22.614613888797336 40-41 12.692396427276623 23.151840743548334 41.8707359678323 22.28502686134274 42-43 18.302269244079696 20.119971655048534 41.86648209488967 19.711277005982105 44-45 10.344088856662601 25.402920141063245 43.81365149467717 20.439339507596983 46-47 17.682344022939255 18.346461883260275 36.28423585247685 27.686958241323623 48-49 17.36515104072249 16.91853853886847 35.35160434417179 30.364706076237248 50-51 13.692693055601332 18.265713061533898 41.70759038924228 26.334003493622493 52-53 12.512977703070154 21.548754964486413 46.76422603450833 19.174041297935105 54-55 11.329554068751854 23.20951847335289 37.94370653571075 27.517220922184503 56-57 17.57358030387924 29.59361919514848 27.82373685771728 25.009063643255004 58-59 8.981246498137834 35.83764543027586 37.43614251343067 17.74496555815563 60-61 15.414785274051612 43.146237764081604 23.00682245146831 18.432154510398473 62-63 8.602221416565044 50.31969941663096 22.151544115223626 18.92653505158037 64-65 8.818100919547806 49.25183744767806 22.036188655614513 19.893872977159617 66-67 9.45749975280973 47.2479483207541 21.73626446063083 21.558287465805346 68-69 10.490755083879899 45.300088988497414 21.606077584786263 22.603078342836426 70-71 11.682212188128275 43.26653702910254 21.33416828713622 23.717082495632972 72-73 12.702284038100261 39.199433110312775 21.676938795689 26.42134405589796 74-75 13.420783757951288 36.874196631620585 21.602781714511718 28.102237895916417 76-77 14.228271975215057 36.775320523384195 21.3292244817244 27.667183019676344 78-79 15.355459609109786 35.95629676015952 20.869450578425234 27.818793052305463 80-81 15.508717576876174 35.48333937576217 20.788701756698856 28.2192412906628 82-83 14.925348538281533 36.04693319270954 21.034244092152534 27.9934741768564 84-85 16.367291783395405 34.21442932006196 21.250123595135296 28.16815530140734 86-87 17.209386638541908 33.76289509244916 20.7738703404634 28.253847928545532 88-89 17.810882963646552 33.66237104907551 21.23694011403711 27.28980587324083 90-91 18.249233710161167 32.84993902639992 22.055963877261792 26.84486338617712 92-93 18.092679872120232 32.302824560825286 22.054315942124518 27.55017962492996 94-95 18.598595959263044 31.302527932500578 22.871691770211925 27.227184338024458 96-97 18.105863353218417 31.417883392109687 23.443525262845654 27.032727991826246 98-99 17.84384166639201 31.772189446623383 23.857156982301177 26.526811904683434 100-101 17.7103589202729 32.319303912198016 24.041725717675753 25.928611449853335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 173.0 1 116.5 2 54.0 3 58.5 4 50.5 5 33.5 6 43.5 7 82.5 8 119.5 9 130.5 10 101.0 11 71.5 12 70.5 13 65.0 14 67.5 15 80.0 16 91.5 17 92.5 18 94.5 19 113.0 20 120.5 21 117.5 22 143.0 23 173.0 24 197.0 25 242.0 26 317.5 27 378.0 28 434.5 29 533.0 30 625.0 31 715.0 32 808.5 33 869.0 34 912.0 35 969.5 36 1022.5 37 1060.5 38 1105.0 39 1181.0 40 1222.0 41 1189.5 42 1148.5 43 1082.0 44 999.0 45 916.5 46 873.5 47 932.0 48 1069.0 49 1221.0 50 1484.0 51 1524.0 52 952.5 53 359.0 54 179.5 55 148.0 56 127.0 57 116.0 58 102.0 59 82.5 60 64.5 61 79.5 62 96.0 63 107.5 64 114.0 65 109.5 66 114.5 67 109.5 68 76.0 69 47.5 70 38.5 71 29.5 72 20.0 73 16.5 74 12.0 75 7.5 76 6.0 77 4.5 78 2.0 79 2.5 80 2.5 81 3.0 82 2.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12194720015820178 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.001647935137272997 44-45 0.0 46-47 0.0 48-49 0.00494380541181899 50-51 0.0 52-53 0.001647935137272997 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 30341.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.17860321017765 #Duplication Level Percentage of deduplicated Percentage of total 1 86.46550344165199 35.60528657592037 2 6.779254041940132 5.583204245080914 3 2.2730910837201854 2.808081473913187 4 1.2245878021450296 2.0170726080221484 5 0.7123419241235793 1.4666622721729674 6 0.4081959340483432 1.0085363040110742 7 0.2881383063870658 0.8305593091855905 8 0.26412678085481034 0.8701097524801424 9 0.16007683688170324 0.5932566494182788 >10 1.1845685929246037 8.486865956955935 >50 0.11205378581719225 3.0915263175241425 >100 0.07203457659676644 6.440130516462872 >500 0.03201536737634064 8.07817804291223 >1k 0.02401152553225548 23.120529975940148 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 3768 12.418839194489305 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 1671 5.507399228766356 RNA PCR Primer, Index 46 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1576 5.194291552684486 RNA PCR Primer, Index 46 (95% over 21bp) CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 782 2.577370554694967 RNA PCR Primer, Index 46 (96% over 26bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 585 1.9280841106094064 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 550 1.8127286510002967 RNA PCR Primer, Index 46 (96% over 28bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 534 1.7599947266075606 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 414 1.3644902936620416 TruSeq Adapter, Index 4 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 1.170033947463828 No Hit ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC 244 0.8041923469892225 TruSeq Adapter, Index 18 (95% over 24bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 236 0.7778253847928546 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 227 0.7481625523219406 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.530635114201905 No Hit CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA 109 0.35924985992551334 RNA PCR Primer, Index 46 (96% over 28bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.35265811937642133 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 101 0.33288289772914537 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.2801489733364095 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC 81 0.2669654922382255 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 80 0.26366962196367955 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 76 0.25048614086549553 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 76 0.25048614086549553 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 74 0.24389440031640355 No Hit GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGC 68 0.2241191786691276 No Hit CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAAGGCG 63 0.20763982729639763 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.20104808674730562 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.19116047592366764 No Hit ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT 56 0.18456873537457566 TruSeq Adapter, Index 4 (96% over 26bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 55 0.18127286510002966 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 54 0.17797699482548368 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 51 0.1680893840018457 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 48 0.1582017731782077 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 45 0.1483141623545697 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT 45 0.1483141623545697 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45 0.1483141623545697 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.14501829208002373 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT 40 0.13183481098183977 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG 40 0.13183481098183977 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 39 0.12853894070729377 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT 39 0.12853894070729377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 38 0.12524307043274777 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.1120595893345638 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 33 0.1087637190600178 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGTCTCT 32 0.1054678487854718 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.04284631356909792 0.0 22-23 0.0 0.0 0.0 0.19280841106094065 0.0 24-25 0.0 0.0 0.0 0.40374410863188426 0.0 26-27 0.0 0.0 0.0 0.7893609307537655 0.0 28-29 0.0 0.0 0.0 1.6446392669984509 0.0 30-31 0.0 0.0 0.0 2.737220263010448 0.0 32-33 0.0 0.0 0.0 3.701262318315151 0.0 34-35 0.0 0.0 0.0 5.0229062984080945 0.0 36-37 0.0 0.0 0.0 6.588444678817442 0.0 38-39 0.0 0.0 0.0 8.17705415114861 0.0 40-41 0.0 0.0 0.0 10.251804488975313 0.0 42-43 0.0 0.0 0.0 11.438317787811872 0.0 44-45 0.0 0.0 0.0 12.12385880491744 0.0 46-47 0.0 0.0 0.0 12.657789789393888 0.0 48-49 0.0 0.0 0.0 13.221383606341254 0.0 50-51 0.0 0.0 0.0 13.705876536699515 0.0 52-53 0.0 0.0 0.0 14.02228008305593 0.0 54-55 0.0 0.0 0.0 14.536435845885105 0.0 56-57 0.0 0.0 0.0 15.193961965657031 0.0 58-59 0.0 0.0 0.0 15.51201344715072 0.0 60-61 0.0 0.0 0.0 15.755907847467125 0.0 62-63 0.0 0.0 0.0 15.9404765828417 0.0 64-65 0.0 0.0 0.0 16.064071718137175 0.0 66-67 0.0 0.0 0.0 16.237104907550837 0.0 68-69 0.0 0.0 0.0 16.50736627006361 0.0 70-71 0.0 0.0 0.0 16.766092086615473 0.0 72-73 0.0 0.0 0.0 16.93088560034277 0.0 74-75 0.0 0.0 0.0 17.082495632971884 0.0 76-77 0.0 0.0 0.0 17.23740153587555 0.0 78-79 0.0 0.0 0.0 17.346165254935563 0.0 80-81 0.0 0.0 0.0 17.451633103721036 0.0 82-83 0.0 0.0 0.0 17.55545301736924 0.0 84-85 0.0 0.0 0.0 17.63949770937016 0.0 86-87 0.0 0.0 0.0 17.738373817606536 0.0 88-89 0.0 0.0 0.0 17.852081342078378 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCATG 30 1.3356566E-6 79.16391 3 GTACCCA 35 3.2729567E-6 68.079475 1 CGCAGAA 65 9.094947E-12 65.766945 9 TACCCAT 40 7.3718547E-6 59.37294 2 GGGTACC 20 5.3485454E-4 47.498352 50-51 CTTATAC 880 0.0 44.94792 1 TTATACA 910 0.0 42.800713 2 GGTATCA 625 0.0 41.93695 1 TATACAC 930 0.0 41.880264 3 ATAAAGG 25 0.0016040369 37.99868 90-91 ATACACA 1115 0.0 35.357513 4 TACACAT 1130 0.0 34.88817 5 CATGGGG 70 2.0071073E-4 33.92739 4 ATGGGTA 30 0.003922411 31.665565 48-49 CAGAGAA 85 1.03682396E-10 30.734226 10-11 GTATCAA 855 0.0 30.554495 2 CACATCT 1325 0.0 29.753683 7 ACACATC 1330 0.0 29.641829 6 ACATCTC 1365 0.0 28.881783 8 GAGTAAA 35 0.008331499 27.141914 12-13 >>END_MODULE