##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031845_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9324
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.645002145002145	31.0	16.0	34.0	7.0	34.0
2	25.61936936936937	31.0	16.0	34.0	7.0	34.0
3	25.614114114114113	31.0	16.0	34.0	7.0	34.0
4	28.118511368511367	35.0	17.0	37.0	7.0	37.0
5	28.21203346203346	35.0	17.0	37.0	7.0	37.0
6	28.505469755469754	35.0	17.0	37.0	8.0	37.0
7	28.455812955812956	35.0	17.0	37.0	8.0	37.0
8	28.546117546117546	35.0	17.0	37.0	8.0	37.0
9	29.676962676962678	37.0	17.0	39.0	8.0	39.0
10-11	29.51329901329901	37.0	17.0	39.0	8.0	39.0
12-13	29.51678464178464	37.0	17.0	39.0	8.0	39.0
14-15	30.143607893607893	38.0	17.0	41.0	8.0	41.0
16-17	30.191387816387817	37.5	17.0	41.0	8.0	41.0
18-19	30.307968682968685	38.0	17.5	41.0	8.0	41.0
20-21	30.335478335478335	38.5	18.0	41.0	8.0	41.0
22-23	30.1498283998284	37.5	17.5	41.0	8.0	41.0
24-25	30.003968253968253	37.5	17.0	41.0	8.0	41.0
26-27	29.60735735735736	36.5	16.0	41.0	8.0	41.0
28-29	29.28946803946804	35.0	16.0	40.5	8.0	41.0
30-31	28.82968682968683	35.0	16.0	40.0	8.0	41.0
32-33	28.304536679536678	35.0	15.0	39.5	8.0	41.0
34-35	27.807646932646932	35.0	13.0	39.5	7.5	41.0
36-37	27.492063492063494	35.0	13.0	39.5	7.0	41.0
38-39	27.0751287001287	35.0	13.0	39.0	7.0	41.0
40-41	26.651758901758903	34.0	12.0	39.0	7.0	41.0
42-43	26.11808236808237	33.0	10.5	38.5	7.0	41.0
44-45	25.55239167739168	32.0	9.0	39.0	7.0	41.0
46-47	24.73916773916774	29.0	8.0	39.0	7.0	41.0
48-49	23.925085800085803	25.5	8.0	39.0	7.0	41.0
50-51	23.013406263406264	23.5	8.0	38.0	7.0	40.0
52-53	22.902885027885027	23.0	8.0	37.5	7.0	39.5
54-55	23.067031317031315	23.0	8.0	38.5	7.0	41.0
56-57	22.833440583440584	22.5	7.5	38.5	7.0	41.0
58-59	22.57957957957958	21.0	7.0	38.0	7.0	41.0
60-61	22.20404332904333	20.0	7.0	37.5	7.0	41.0
62-63	21.76474688974689	20.0	7.0	36.5	7.0	41.0
64-65	21.484770484770486	19.5	7.0	36.0	7.0	40.5
66-67	21.01539039039039	18.0	7.0	35.0	7.0	39.0
68-69	20.580169455169454	17.5	7.0	35.0	7.0	39.0
70-71	20.015336765336762	15.0	7.0	35.0	7.0	37.0
72-73	19.54912054912055	13.5	7.0	34.5	7.0	36.5
74-75	19.197983697983698	13.0	7.0	34.0	7.0	35.5
76-77	18.939886314886316	13.0	7.0	34.0	7.0	35.0
78-79	18.58891033891034	12.0	7.0	34.0	6.0	35.0
80-81	18.351190476190474	11.0	7.0	33.0	5.5	35.0
82-83	18.032175032175033	11.0	7.0	32.5	5.0	35.0
84-85	17.65588803088803	10.0	7.0	32.0	5.0	35.0
86-87	17.473670098670098	10.0	7.0	31.5	5.0	35.0
88-89	17.181038181038183	10.0	7.0	31.0	5.0	35.0
90-91	16.988416988416986	10.0	7.0	30.0	5.0	35.0
92-93	16.658515658515658	10.0	7.0	29.0	5.0	35.0
94-95	16.455062205062205	10.0	7.0	29.0	5.0	35.0
96-97	16.171546546546544	10.0	7.0	27.5	5.0	35.0
98-99	15.973455598455597	10.0	7.0	27.0	5.0	35.0
100-101	14.997854997854997	9.0	7.0	25.0	5.0	33.5
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.09987018190144781
1101	2	0.8931870502183052
1101	3	1.6076804561179507
1101	4	1.7701563771876216
1101	5	1.4456788870851405
1101	6	3.556455205673945
1101	7	3.119533331252075
1101	8	1.0218759828134836
1101	9	0.8281761352073858
1101	10-11	1.3124313909470118
1101	12-13	1.9242028515465996
1101	14-15	3.00282584891961
1101	16-17	2.498203908360157
1101	18-19	3.1134507853257816
1101	20-21	2.6129013058700643
1101	22-23	2.704559835419218
1101	24-25	2.2750171703296758
1101	26-27	-0.04844659102471027
1101	28-29	1.5269142749611397
1101	30-31	2.9274178801522446
1101	32-33	0.3884662212787333
1101	34-35	2.7944746370527582
1101	36-37	3.162467899577276
1101	38-39	3.1075777376558733
1101	40-41	-1.465300339128465
1101	42-43	-3.4099231353137753
1101	44-45	-2.0814170782920804
1101	46-47	-1.9873957350519937
1101	48-49	-4.344699975949975
1101	50-51	-1.551788662726164
1101	52-53	-1.6205917202011015
1101	54-55	-2.650744351134975
1101	56-57	-2.930124432858804
1101	58-59	-3.6858631241443724
1101	60-61	-3.7311522100584646
1101	62-63	-2.7339078889860176
1101	64-65	-4.066707525691903
1101	66-67	-2.3139738444425895
1101	68-69	-1.2048940353627877
1101	70-71	-1.6483386405261413
1101	72-73	-3.0982512712981425
1101	74-75	-1.3766740348771584
1101	76-77	-1.254136849839977
1101	78-79	-0.316234214281085
1101	80-81	0.4833232450419871
1101	82-83	-0.3086833016520494
1101	84-85	-0.8669484017140299
1101	86-87	-2.3774404848623654
1101	88-89	-0.21837845314408355
1101	90-91	-1.2839400038618827
1101	92-93	0.3678808460058498
1101	94-95	-0.23517009610760198
1101	96-97	1.4340851422882661
1101	98-99	0.39799822573259824
1101	100-101	-0.7544493267149548
1102	1	-1.7248701819014478
1102	2	-1.4818129497816948
1102	3	-1.6423195438820493
1102	4	-1.1048436228123784
1102	5	0.19567888708514047
1102	6	1.3064552056739451
1102	7	0.4945333312520752
1102	8	-2.8531240171865164
1102	9	1.4531761352073858
1102	10-11	-0.4375686090529882
1102	12-13	0.6742028515465996
1102	14-15	-1.3721741510803902
1102	16-17	0.12320390836015704
1102	18-19	-1.5740492146742184
1102	20-21	-2.4495986941299357
1102	22-23	-2.982940164580782
1102	24-25	-1.4749828296703242
1102	26-27	-3.6109465910247103
1102	28-29	-5.22308572503886
1102	30-31	-2.4475821198477554
1102	32-33	-3.6115337787212667
1102	34-35	-3.1430253629472418
1102	36-37	-5.712532100422724
1102	38-39	-3.8924222623441267
1102	40-41	-4.402800339128465
1102	42-43	-4.284923135313775
1102	44-45	-4.3314170782920804
1102	46-47	-0.42489573505199374
1102	48-49	-0.5321999759499754
1102	50-51	-3.926788662726164
1102	52-53	-5.1830917202011015
1102	54-55	-5.400744351134975
1102	56-57	-2.992624432858804
1102	58-59	-3.3108631241443724
1102	60-61	-2.1061522100584646
1102	62-63	-7.2339078889860176
1102	64-65	-5.316707525691903
1102	66-67	-2.8139738444425895
1102	68-69	-0.07989403536278772
1102	70-71	-0.5858386405261413
1102	72-73	-0.4107512712981425
1102	74-75	-3.1266740348771584
1102	76-77	-1.004136849839977
1102	78-79	-1.128734214281085
1102	80-81	-2.079176754958013
1102	82-83	-1.4961833016520494
1102	84-85	-0.6169484017140299
1102	86-87	-1.0649404848623654
1102	88-89	-3.5308784531440836
1102	90-91	-1.5339400038618827
1102	92-93	0.4928808460058498
1102	94-95	0.389829903892398
1102	96-97	0.9965851422882661
1102	98-99	1.2104982257325982
1102	100-101	-1.0044493267149548
1103	1	4.060844103812837
1103	2	1.4646156216468782
1103	3	2.3041090275465237
1103	4	3.9665849486161946
1103	5	2.1599646013708558
1103	6	1.752883777102518
1103	7	-0.18403809731935183
1103	8	2.4683045542420565
1103	9	2.9531761352073858
1103	10-11	2.5445742480898694
1103	12-13	2.638488565832315
1103	14-15	3.4135401346339016
1103	16-17	4.917846765503018
1103	18-19	2.7205936424686428
1103	20-21	4.621829877298634
1103	22-23	3.847416978276364
1103	24-25	4.105374313186815
1103	26-27	3.7372676946895744
1103	28-29	4.009057132103994
1103	30-31	3.909560737295106
1103	32-33	5.9152519355644415
1103	34-35	4.312331779909901
1103	36-37	5.698182185291561
1103	38-39	4.089720594798727
1103	40-41	2.9275568037286774
1103	42-43	0.7061482932576553
1103	44-45	0.17751149313648895
1103	46-47	0.08403283637657566
1103	48-49	-4.300057118807118
1103	50-51	-2.2928600912975945
1103	52-53	-1.8795202916296745
1103	54-55	-7.025744351134975
1103	56-57	-7.314053004287377
1103	58-59	-10.784077409858657
1103	60-61	-10.552580781487036
1103	62-63	-9.546407888986018
1103	64-65	-9.638136097120475
1103	66-67	-11.778259558728303
1103	68-69	-9.231679749648501
1103	70-71	-9.514410069097568
1103	72-73	-9.178608414155285
1103	74-75	-10.82310260630573
1103	76-77	-10.370208278411406
1103	78-79	-5.6555199285667985
1103	80-81	-8.829176754958013
1103	82-83	-9.397969015937765
1103	84-85	-6.5990912588568875
1103	86-87	-6.957797627719508
1103	88-89	-9.013021310286941
1103	90-91	-7.757154289576169
1103	92-93	-7.13211915399415
1103	94-95	-7.681598667536173
1103	96-97	-6.744486286283163
1103	98-99	-6.735930345695975
1103	100-101	-6.46873504100067
1104	1	-1.5105844676157325
1104	2	-0.10681294978169475
1104	3	-0.4101766867391916
1104	4	-3.461986479955236
1104	5	-4.840035398629144
1104	6	-3.389973365754628
1104	7	-6.755466668747925
1104	8	-3.5316954457579435
1104	9	-7.046823864792614
1104	10-11	-3.5268543233387035
1104	12-13	-3.2186542913105427
1104	14-15	-3.37217415108039
1104	16-17	-3.796438948782697
1104	18-19	-3.2794063575313643
1104	20-21	-4.163884408415651
1104	22-23	-4.581154450295067
1104	24-25	-7.680339972527467
1104	26-27	-6.191303733881853
1104	28-29	-6.34808572503886
1104	30-31	-4.161867834133471
1104	32-33	-2.4418909215784126
1104	34-35	-4.544811077232957
1104	36-37	0.34103932814870674
1104	38-39	-6.695993690915557
1104	40-41	-2.643871767699892
1104	42-43	-2.650994563885206
1104	44-45	-1.9653456497206498
1104	46-47	-0.5588243064805631
1104	48-49	-1.514342833092833
1104	50-51	-1.0071458055833062
1104	52-53	0.6204797083703255
1104	54-55	2.1885413631507404
1104	56-57	1.757375567141196
1104	58-59	-0.6412202670015148
1104	60-61	-1.4811522100584646
1104	62-63	-1.2606936032717329
1104	64-65	-1.5667075256919034
1104	66-67	0.15031186984312583
1104	68-69	0.6254631074943582
1104	70-71	0.9855899309024281
1104	72-73	-0.8928941284410001
1104	74-75	2.6054688222656956
1104	76-77	3.6297917215885924
1104	78-79	1.3444800714331997
1104	80-81	-0.5434624692437282
1104	82-83	0.03060241263366592
1104	84-85	-2.0990912588568875
1104	86-87	0.756488086566204
1104	88-89	-0.2987355960012259
1104	90-91	1.385702853280975
1104	92-93	-1.0606905825655808
1104	94-95	1.1755441896066827
1104	96-97	2.1126565708596967
1104	98-99	2.5497839400183135
1104	100-101	-1.46873504100067
1105	1	-6.224870181901448
1105	2	-6.2179240608928055
1105	3	-6.489541766104271
1105	4	-5.715954733923489
1105	5	-8.220987779581527
1105	6	-6.262989238770498
1105	7	-9.088800002081257
1105	8	-5.769790683853184
1105	9	-11.269046087014836
1105	10-11	-5.090346386830767
1105	12-13	-7.520241592897843
1105	14-15	-8.538840817747058
1105	16-17	-7.605962758306507
1105	18-19	-8.136549214674218
1105	20-21	-8.005154249685493
1105	22-23	-6.406551275691893
1105	24-25	-6.9263717185592135
1105	26-27	-5.1595577021358245
1105	28-29	-6.84808572503886
1105	30-31	-5.947582119847755
1105	32-33	-5.576811556499045
1105	34-35	-6.4416364740583525
1105	36-37	-7.198643211533835
1105	38-39	-5.100755595677462
1105	40-41	-7.159744783572908
1105	42-43	-4.000200913091554
1105	44-45	-6.338361522736523
1105	46-47	-6.654062401718658
1105	48-49	-5.046088864838865
1105	50-51	-3.364288662726164
1105	52-53	-4.58586949797888
1105	54-55	-2.1924110178016427
1105	56-57	-1.9092910995254684
1105	58-59	-5.16502979081104
1105	60-61	-5.036707765614022
1105	62-63	-6.601963444541573
1105	64-65	-5.844485303469682
1105	66-67	-4.1750849555537
1105	68-69	-4.406282924251677
1105	70-71	-5.86361641830392
1105	72-73	-5.5913068268537
1105	74-75	-4.418340701543826
1105	76-77	-6.2194146276177555
1105	78-79	-7.274567547614417
1105	80-81	-3.718065643846902
1105	82-83	-4.683683301652049
1105	84-85	-6.24194840171403
1105	86-87	-4.037162707084587
1105	88-89	-5.6558784531440836
1105	90-91	-6.693662226084104
1105	92-93	-5.798785820660818
1105	94-95	-5.999058984996491
1105	96-97	-4.649248191045066
1105	98-99	-5.497835107600734
1105	100-101	-5.254449326714955
1106	1	3.9751298180985515
1106	2	6.793187050218304
1106	3	7.032680456117948
1106	4	7.5951563771876245
1106	5	5.845678887085139
1106	6	6.181455205673945
1106	7	7.844533331252077
1106	8	6.296875982813482
1106	9	5.753176135207383
1106	10-11	6.537431390947013
1106	12-13	6.9242028515466
1106	14-15	7.37782584891961
1106	16-17	7.210703908360163
1106	18-19	7.363450785325782
1106	20-21	6.050401305870064
1106	22-23	5.104559835419224
1106	24-25	7.762517170329673
1106	26-27	5.851553408975288
1106	28-29	3.351914274961139
1106	30-31	2.5024178801522474
1106	32-33	3.650966221278736
1106	34-35	4.469474637052759
1106	36-37	4.762467899577278
1106	38-39	4.732577737655873
1106	40-41	1.6346996608715365
1106	42-43	3.127576864686226
1106	44-45	5.056082921707922
1106	46-47	2.0626042649480034
1106	48-49	1.0428000240500275
1106	50-51	1.5357113372738347
1106	52-53	0.4919082797988956
1106	54-55	1.9242556488650244
1106	56-57	1.0573755671411966
1106	58-59	3.451636875855627
1106	60-61	0.7688477899415354
1106	62-63	1.0035921110139832
1106	64-65	2.4832924743080937
1106	66-67	1.2360261555574077
1106	68-69	0.9826059646372123
1106	70-71	0.5641613594738573
1106	72-73	-1.935751271298141
1106	74-75	-1.0516740348771556
1106	76-77	2.2083631501600216
1106	78-79	-1.7912342142810864
1106	80-81	-3.279176754958012
1106	82-83	-2.333683301652048
1106	84-85	1.1580515982859687
1106	86-87	-2.414940484862367
1106	88-89	-1.5558784531440821
1106	90-91	-2.8714400038618813
1106	92-93	-2.9821191539941516
1106	94-95	-1.860170096107602
1106	96-97	0.034085142288267534
1106	98-99	2.5354982257325975
1106	100-101	-0.4544493267149541
1107	1	0.44179648476522004
1107	2	-0.6623685053372519
1107	3	1.8437915672290615
1107	4	1.1729341549654002
1107	5	0.7790122204184726
1107	6	1.4036774278961666
1107	7	2.4667555534742966
1107	8	1.6746537605912621
1107	9	3.7309539129851643
1107	10-11	2.6874313909470118
1107	12-13	1.2575361848799353
1107	14-15	5.794492515586281
1107	16-17	2.94959279724905
1107	18-19	1.3634507853257816
1107	20-21	1.7726235280922857
1107	22-23	3.204559835419218
1107	24-25	2.2958505036630115
1107	26-27	3.118220075641954
1107	28-29	3.818580941627811
1107	30-31	2.441306769041134
1107	32-33	4.923188443500955
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1309	4	0.4860654680967116
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1309	6	1.4541824784012185
1309	7	1.5172606039793486
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1309	9	-0.6831875011562509
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1310	4	2.576974559005805
1310	5	3.627497068903324
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1310	7	3.1536242403429853
1310	8	3.5332396191771203
1310	9	1.7713579533892023
1310	10-11	2.3237950273106485
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1310	14-15	3.2187349398287033
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1310	32-33	2.7009662212787333
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1310	52-53	4.1919082797988985
1310	54-55	3.383346557955935
1310	56-57	3.075557385323016
1310	58-59	1.592545966764721
1310	60-61	1.9733932444869886
1310	62-63	2.044501201923076
1310	64-65	2.2514742924899167
1310	66-67	2.799662519193774
1310	68-69	2.4371514191826655
1310	70-71	2.5505249958374954
1310	72-73	1.8278850923382208
1310	74-75	1.021053237850115
1310	76-77	3.6038176956145698
1310	78-79	3.9224021493552783
1310	80-81	5.216277790496534
1310	82-83	5.225407607438857
1310	84-85	3.1216879619223334
1310	86-87	3.775968606046728
1310	88-89	3.571394274128643
1310	90-91	2.210378177956297
1310	92-93	2.4133353914603966
1310	94-95	1.389829903892398
1310	96-97	1.6386305968337194
1310	98-99	2.6991345893689616
1310	100-101	1.927368855103225
1311	1	-0.12487018190144639
1311	2	-0.40681294978169547
1311	3	0.8326804561179522
1311	4	-1.1048436228123784
1311	5	0.5456788870851419
1311	6	-0.8185447943260549
1311	7	-0.2554666687479248
1311	8	-0.8031240171865157
1311	9	0.7531761352073865
1311	10-11	1.1374313909470146
1311	12-13	1.624202851546599
1311	14-15	0.8778258489196098
1311	16-17	-0.6392960916398458
1311	18-19	-0.18654921467421914
1311	20-21	2.950401305870063
1311	22-23	1.3545598354192165
1311	24-25	1.5125171703296765
1311	26-27	1.901553408975289
1311	28-29	-0.4480857250388617
1311	30-31	0.45241788015224316
1311	32-33	2.3009662212787347
1311	34-35	1.9694746370527554
1311	36-37	1.4624678995772769
1311	38-39	1.0825777376558747
1311	40-41	0.18469966087153367
1311	42-43	0.17757686468622325
1311	44-45	-0.7439170782920819
1311	46-47	-0.8873957350519923
1311	48-49	1.292800024050024
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1311	52-53	1.4419082797988985
1311	54-55	0.47425564886502514
1311	56-57	0.8573755671411973
1311	58-59	1.2516368758556276
1311	60-61	0.9188477899415339
1311	62-63	-0.046407888986017554
1311	64-65	0.33329247430809517
1311	66-67	0.9860261555574077
1311	68-69	1.5826059646372137
1311	70-71	2.0641613594738573
1311	72-73	4.014248728701855
1311	74-75	1.348325965122843
1311	76-77	1.8583631501600202
1311	78-79	2.9087657857189164
1311	80-81	2.420823245041987
1311	82-83	2.666316698347952
1311	84-85	2.50805159828597
1311	86-87	1.2350595151376318
1311	88-89	2.444121546855918
1311	90-91	1.928559996138116
1311	92-93	2.7178808460058512
1311	94-95	1.6898299038923987
1311	96-97	3.0340851422882675
1311	98-99	4.1854982257326
1311	100-101	2.645550673285044
1312	1	2.075129818098553
1312	2	3.4931870502183067
1312	3	2.43268045611795
1312	4	4.495156377187623
1312	5	1.545678887085142
1312	6	2.8814552056739444
1312	7	2.644533331252074
1312	8	1.0968759828134829
1312	9	0.25317613520738647
1312	10-11	2.6874313909470118
1312	12-13	-0.32579714845340035
1312	14-15	0.3278258489196091
1312	16-17	-0.48929609163984367
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1312	20-21	2.0504013058700643
1312	22-23	2.2545598354192187
1312	24-25	3.1625171703296786
1312	26-27	3.6015534089752883
1312	28-29	3.5519142749611383
1312	30-31	5.802417880152245
1312	32-33	2.7009662212787333
1312	34-35	1.7694746370527596
1312	36-37	2.4624678995772733
1312	38-39	2.132577737655872
1312	40-41	2.534699660871535
1312	42-43	4.327576864686225
1312	44-45	3.8560829217079196
1312	46-47	6.012604264948006
1312	48-49	1.692800024050026
1312	50-51	-1.5142886627261625
1312	52-53	-1.2580917202011008
1312	54-55	-0.8757443511349763
1312	56-57	0.3073755671411966
1312	58-59	-3.098363124144374
1312	60-61	-1.3811522100584632
1312	62-63	-0.046407888986017554
1312	64-65	-0.216707525691902
1312	66-67	1.3360261555574091
1312	68-69	0.9326059646372116
1312	70-71	-0.43583864052614274
1312	72-73	1.7142487287018575
1312	74-75	0.7483259651228416
1312	76-77	-2.2416368498399777
1312	78-79	1.1087657857189157
1312	80-81	-1.3791767549580136
1312	82-83	0.4663166983479492
1312	84-85	2.00805159828597
1312	86-87	1.2350595151376353
1312	88-89	1.3441215468559164
1312	90-91	1.8785599961381187
1312	92-93	0.5678808460058526
1312	94-95	1.2898299038923966
1312	96-97	0.28408514228826753
1312	98-99	-1.5145017742674014
1312	100-101	0.4455506732850445
1313	1	-4.315779272810538
1313	2	-0.8340856770544214
1313	3	-0.26731954388204926
1313	4	-2.150298168266925
1313	5	-4.281593840187586
1313	6	-3.818544794326055
1313	7	-2.028193941475198
1313	8	-1.3758512899137898
1313	9	-0.6831875011562509
1313	10-11	-1.6762049726893515
1313	12-13	-1.303069875726127
1313	14-15	-1.7358105147167535
1313	16-17	-3.5756597280034796
1313	18-19	-4.4547310328560386
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1313	22-23	-3.0681674373080554
1313	24-25	-2.1738464660339574
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1313	28-29	-3.8480857250388603
1313	30-31	-2.3566730289386655
1313	32-33	-3.48085196053945
1313	34-35	-4.785070817492695
1313	36-37	-7.042077554968177
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1313	84-85	-6.105584765350393
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1313	92-93	-2.4048464267214236
1313	94-95	-3.7919882779257836
1313	96-97	-5.725005766802644
1313	98-99	-5.709956319721947
1313	100-101	-4.163540235805865
1314	1	-2.30820351523478
1314	2	-1.7734796164483626
1314	3	0.23268045611795074
1314	4	-0.35484362281237836
1314	5	-2.1376544462481917
1314	6	-3.651878127659387
1314	7	-3.1721333354145926
1314	8	-1.7697906838531843
1314	9	-4.546823864792614
1314	10-11	-0.3959019423863239
1314	12-13	-2.7007971484534004
1314	14-15	-1.6638408177470545
1314	16-17	-1.564296091639843
1314	18-19	-3.5532158813408827
1314	20-21	-1.9079320274632678
1314	22-23	-1.795440164580782
1314	24-25	-2.62081616300366
1314	26-27	-2.2151132576913746
1314	28-29	-2.6397523917055246
1314	30-31	-3.3225821198477554
1314	32-33	-1.6740337787212667
1314	34-35	-2.288858696280574
1314	36-37	-3.337532100422724
1314	38-39	-2.1840889290107945
1314	40-41	-3.9236336724618006
1314	42-43	-4.972423135313775
1314	44-45	-5.685583744958745
1314	46-47	-7.695729068385326
1314	48-49	-5.657199975949975
1314	50-51	-4.5726219960595
1314	52-53	-4.599758386867769
1314	54-55	-4.359077684468307
1314	56-57	-3.659291099525472
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1314	60-61	-5.356152210058465
1314	62-63	-4.9214078889860176
1314	64-65	-5.608374192358571
1314	66-67	-5.772307177775922
1314	68-69	-5.017394035362788
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1314	72-73	-4.5357512712981425
1314	74-75	-4.0016740348771584
1314	76-77	-1.7749701831733127
1314	78-79	-2.857900880947753
1314	80-81	-2.787510088291347
1314	82-83	-2.0170166349853833
1314	84-85	-4.325281735047364
1314	86-87	-2.4399404848623654
1314	88-89	-3.1142117864774157
1314	90-91	-3.554773337195215
1314	92-93	-2.7154524873274823
1314	94-95	-4.985170096107602
1314	96-97	-2.274248191045068
1314	98-99	-3.081168440934068
1314	100-101	-2.629449326714955
1315	1	-5.532562489593754
1315	2	-1.5683514113201547
1315	3	-4.113473390035896
1315	4	-5.297151315120072
1315	5	-5.015859574453319
1315	6	-5.433929409710672
1315	7	-4.140082053363308
1315	8	-3.1031240171865164
1315	9	-4.739131557100308
1315	10-11	-5.081799378283758
1315	12-13	-4.268104840761094
1315	14-15	-6.064481843388084
1315	16-17	-6.7469883993321496
1315	18-19	-4.9057799839049885
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1315	22-23	-4.180055549196169
1315	24-25	-3.1144059065934044
1315	26-27	-3.894600437178557
1315	28-29	-4.3865472635004
1315	30-31	-4.562966735232369
1315	32-33	-5.875956855644343
1315	34-35	-4.792063824485702
1315	36-37	-3.1644551773458005
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1315	62-63	-1.4310232736014008
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1315	76-77	2.1737477655446362
1315	78-79	0.4433811703343018
1315	80-81	-1.2522536780349363
1315	82-83	-0.991375609344356
1315	84-85	-0.5496407094063365
1315	86-87	0.8004441305222478
1315	88-89	2.151813854548223
1315	90-91	0.7593292269073473
1315	92-93	0.8294193075443097
1315	94-95	1.120599134661628
1315	96-97	0.6840851422882661
1315	98-99	1.681652071886445
1315	100-101	1.9763199040542787
1316	1	-1.939155896187163
1316	2	-2.749670092638837
1316	3	-2.553033829596334
1316	4	-3.0334150513838054
1316	5	-1.4828925414862866
1316	6	-3.1042590800403396
1316	7	-0.7554666687479248
1316	8	0.8968759828134836
1316	9	-1.9753952933640413
1316	10-11	-3.0268543233387035
1316	12-13	-1.6472257198819733
1316	14-15	-1.8364598653661055
1316	16-17	-1.046438948782697
1316	18-19	-0.029406357531360783
1316	20-21	-2.2353129798442204
1316	22-23	-2.8311544502950667
1316	24-25	-5.2517685439560395
1316	26-27	-2.905589448167568
1316	28-29	-3.1695142964674297
1316	30-31	-3.1975821198477554
1316	32-33	-1.441890921578409
1316	34-35	-3.1162396486615265
1316	36-37	-3.7661035289941545
1316	38-39	-3.6959936909155573
1316	40-41	-1.0367289105570343
1316	42-43	-1.9724231353137753
1316	44-45	-1.7153456497206498
1316	46-47	-0.8802528779091396
1316	48-49	1.5928000240500246
1316	50-51	0.5285684801309785
1316	52-53	-0.1652345773439592
1316	54-55	0.7956842202935945
1316	56-57	1.7930898528554806
1316	58-59	2.180208304427058
1316	60-61	1.6617049327986813
1316	62-63	1.882163539585413
1316	64-65	3.254721045736666
1316	66-67	1.8645975841288411
1316	68-69	4.411177393208643
1316	70-71	1.6998756451881434
1316	72-73	0.07139158584471517
1316	74-75	0.35546882226569565
1316	76-77	-0.8344939926971229
1316	78-79	-0.3698056428525156
1316	80-81	-0.7934624692437282
1316	82-83	1.4234595554908047
1316	84-85	-0.3490912588568875
1316	86-87	1.9350595151376346
1316	88-89	0.7012644039987741
1316	90-91	2.064274281852402
1316	92-93	2.2250237031487075
1316	94-95	1.6041156181781133
1316	96-97	0.9340851422882661
1316	98-99	-0.3430732028388306
1316	100-101	-0.2901636124292395
2101	1	-0.05820351523477996
2101	2	2.3931870502183052
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2208	82-83	-5.33368330165205
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2208	94-95	-5.710170096107603
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2209	4	-4.513934531903288
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2209	6	-3.0912720670533282
2209	7	-5.846375759657015
2209	8	-2.2849421990047
2209	9	-4.228642046610798
2209	10-11	-2.539841336325715
2209	12-13	-2.666706239362494
2209	14-15	-2.9630832419894837
2209	16-17	-3.5756597280034796
2209	18-19	-4.227458305583312
2209	20-21	-2.358689603220842
2209	22-23	1.022741653601038
2209	24-25	-3.4920282842157775
2209	26-27	-3.4120829546610736
2209	28-29	-0.9389948159479502
2209	30-31	-1.4475821198477554
2209	32-33	-3.57176105144854
2209	34-35	-0.3759799084017885
2209	36-37	-0.9966230095136304
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2209	40-41	-3.0789367027648282
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2209	48-49	-0.7026545214045221
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2209	56-57	1.9391937489593758
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2209	64-65	0.34238338339900665
2209	66-67	0.7542079737392307
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2209	82-83	-0.36550148347022926
2209	84-85	-0.24194840171402987
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2209	100-101	0.20009612783050024
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2310	60-61	11.074403345497092
2310	62-63	11.175814333236204
2310	64-65	10.044403585419207
2310	66-67	10.93602615555741
2310	68-69	12.093717075748327
2310	70-71	11.13638358169608
2310	72-73	11.353137617590747
2310	74-75	10.192770409567284
2310	76-77	10.780585372382244
2310	78-79	11.003210230163358
2310	80-81	11.670823245041987
2310	82-83	10.427427809459065
2310	84-85	11.369162709397084
2310	86-87	11.407281737359856
2310	88-89	11.455232657967027
2310	90-91	12.139671107249228
2310	92-93	11.034547512672514
2310	94-95	10.667607681670177
2310	96-97	9.184085142288266
2310	98-99	10.002164892399263
2310	100-101	8.023328451062824
2311	1	2.775129818098552
2311	2	0.7681870502183052
2311	3	2.6076804561179507
2311	4	3.6451563771876216
2311	5	-0.6793211129148595
2311	6	2.431455205673945
2311	7	2.744533331252075
2311	8	5.646875982813484
2311	9	3.5781761352073858
2311	10-11	1.5624313909470118
2311	12-13	3.2992028515465996
2311	14-15	2.75282584891961
2311	16-17	4.560703908360157
2311	18-19	4.488450785325782
2311	20-21	4.175401305870064
2311	22-23	0.517059835419218
2311	24-25	0.6500171703296758
2311	26-27	1.0765534089752897
2311	28-29	1.3394142749611397
2311	30-31	1.8024178801522446
2311	32-33	-0.8615337787212667
2311	34-35	2.4819746370527582
2311	36-37	6.099967899577276
2311	38-39	3.5450777376558733
2311	40-41	4.284699660871535
2311	42-43	1.2775768646862247
2311	44-45	0.9810829217079196
2311	46-47	3.8876042649480063
2311	48-49	7.217800024050025
2311	50-51	6.010711337273836
2311	52-53	4.5669082797988985
2311	54-55	4.411755648865025
2311	56-57	4.632375567141196
2311	58-59	4.8141368758556276
2311	60-61	5.081347789941535
2311	62-63	3.6410921110139824
2311	64-65	4.933292474308097
2311	66-67	4.6860261555574105
2311	68-69	8.045105964637212
2311	70-71	6.851661359473859
2311	72-73	7.6517487287018575
2311	74-75	7.1858259651228416
2311	76-77	3.995863150160023
2311	78-79	4.871265785718915
2311	80-81	5.108323245041987
2311	82-83	6.128816698347951
2311	84-85	6.19555159828597
2311	86-87	6.185059515137635
2311	88-89	4.4066215468559164
2311	90-91	7.403559996138117
2311	92-93	5.80538084600585
2311	94-95	5.014829903892398
2311	96-97	5.434085142288266
2311	98-99	7.272998225732598
2311	100-101	6.120550673285045
2312	1	2.6084631514318843
2312	2	3.809853716884973
2312	3	4.816013789451283
2312	4	4.978489710520957
2312	5	4.612345553751805
2312	6	5.181455205673945
2312	7	0.5778666645854074
2312	8	2.3968759828134836
2312	9	3.86984280187405
2312	10-11	2.7707647242803475
2312	12-13	1.1742028515465996
2312	14-15	2.544492515586274
2312	16-17	2.810703908360157
2312	18-19	1.4467841186591137
2312	20-21	1.9670679725367322
2312	22-23	2.7878931687525466
2312	24-25	3.8375171703296758
2312	26-27	2.1598867423086254
2312	28-29	1.9019142749611397
2312	30-31	1.5524178801522446
2312	32-33	1.909299554612069
2312	34-35	1.502807970386094
2312	36-37	2.704134566243944
2312	38-39	0.3992444043225376
2312	40-41	1.3263663275381994
2312	42-43	0.7359101980195568
2312	44-45	2.9810829217079196
2312	46-47	6.554270931614671
2312	48-49	5.217800024050025
2312	50-51	4.219044670607172
2312	52-53	3.9419082797988985
2312	54-55	5.390922315531693
2312	56-57	2.9657089004745316
2312	58-59	5.168303542522295
2312	60-61	7.893847789941535
2312	62-63	6.9535921110139824
2312	64-65	5.349959140974761
2312	66-67	5.644359488890746
2312	68-69	5.649272631303877
2312	70-71	5.455828026140523
2312	72-73	6.4642487287018575
2312	74-75	6.664992631789506
2312	76-77	4.808363150160023
2312	78-79	6.767099119052251
2312	80-81	6.254156578375323
2312	82-83	3.9413166983479506
2312	84-85	3.13305159828597
2312	86-87	5.060059515137635
2312	88-89	3.9274548801892486
2312	90-91	3.6535599961381173
2312	92-93	0.576214179339182
2312	94-95	3.97316323722573
2312	96-97	0.6840851422882661
2312	98-99	3.4604982257325982
2312	100-101	1.1205506732850452
2313	1	3.1084631514318843
2313	2	2.809853716884973
2313	3	3.7326804561179507
2313	4	5.311823043854286
2313	5	6.362345553751805
2313	6	6.181455205673945
2313	7	6.9111999979187395
2313	8	2.6468759828134836
2313	9	4.7865094685407215
2313	10-11	3.6874313909470118
2313	12-13	4.715869518213271
2313	14-15	6.12782584891961
2313	16-17	7.269037241693486
2313	18-19	3.6134507853257816
2313	20-21	6.258734639203393
2313	22-23	6.829559835419218
2313	24-25	5.004183836996347
2313	26-27	4.82655340897529
2313	28-29	4.52691427496114
2313	30-31	5.760751213485573
2313	32-33	5.700966221278733
2313	34-35	3.4611413037194225
2313	36-37	3.8291345662439404
2313	38-39	5.107577737655873
2313	40-41	5.784699660871535
2313	42-43	5.944243531352889
2313	44-45	7.2310829217079196
2313	46-47	7.637604264948006
2313	48-49	8.217800024050025
2313	50-51	5.760711337273836
2313	52-53	4.3169082797988985
2313	54-55	0.8909223155316894
2313	56-57	3.340708900474528
2313	58-59	7.168303542522292
2313	60-61	7.477181123274871
2313	62-63	9.370258777680647
2313	64-65	8.433292474308097
2313	66-67	7.769359488890746
2313	68-69	8.565939297970548
2313	70-71	9.830828026140527
2313	72-73	8.464248728701858
2313	74-75	8.914992631789506
2313	76-77	6.933363150160023
2313	78-79	8.058765785718915
2313	80-81	7.087489911708655
2313	82-83	8.982983365014615
2313	84-85	8.50805159828597
2313	86-87	7.476726181804299
2313	88-89	7.5941215468559164
2313	90-91	6.528559996138117
2313	92-93	5.49288084600585
2313	94-95	4.306496570559062
2313	96-97	5.184085142288266
2313	98-99	4.918831559065934
2313	100-101	2.5788840066183774
2314	1	2.4751298180985515
2314	2	3.0931870502183045
2314	3	1.3326804561179522
2314	4	0.9951563771876231
2314	5	2.6456788870851398
2314	6	-1.5185447943260542
2314	7	-0.9554666687479241
2314	8	0.7968759828134822
2314	9	-1.0468238647926142
2314	10-11	1.987431390947009
2314	12-13	2.9742028515466004
2314	14-15	0.07782584891960909
2314	16-17	-2.1392960916398422
2314	18-19	2.1634507853257787
2314	20-21	-0.09959869412993427
2314	22-23	-1.8454401645807827
2314	24-25	0.36251717032967434
2314	26-27	-7.79844659102471
2314	28-29	-0.5480857250388631
2314	30-31	1.3524178801522453
2314	32-33	0.30096622127873474
2314	34-35	-0.3805253629472425
2314	36-37	-0.48753210042272244
2314	38-39	-0.9174222623441253
2314	40-41	0.43469966087153367
2314	42-43	2.0775768646862254
2314	44-45	0.9060829217079203
2314	46-47	-1.1373957350519923
2314	48-49	-0.7571999759499768
2314	50-51	2.385711337273836
2314	52-53	4.0419082797989
2314	54-55	2.8242556488650266
2314	56-57	7.957375567141195
2314	58-59	4.351636875855629
2314	60-61	4.5688477899415325
2314	62-63	2.003592111013983
2314	64-65	2.2332924743080937
2314	66-67	4.886026155557413
2314	68-69	6.682605964637212
2314	70-71	2.864161359473858
2314	72-73	0.9642487287018575
2314	74-75	0.5483259651228423
2314	76-77	2.60836315016002
2314	78-79	2.1587657857189164
2314	80-81	2.3208232450419857
2314	82-83	3.1163166983479513
2314	84-85	0.6080515982859716
2314	86-87	1.3350595151376332
2314	88-89	4.5941215468559164
2314	90-91	3.4785599961381166
2314	92-93	2.817880846005849
2314	94-95	0.5898299038923973
2314	96-97	-0.16591485771173353
2314	98-99	-0.614501774267401
2314	100-101	1.5955506732850466
2315	1	2.3751298180985536
2315	2	2.3931870502183052
2315	3	-0.6673195438820478
2315	4	1.2951563771876202
2315	5	1.545678887085142
2315	6	-1.8185447943260549
2315	7	2.244533331252075
2315	8	3.3968759828134836
2315	9	2.9531761352073858
2315	10-11	2.537431390947013
2315	12-13	3.0742028515465982
2315	14-15	2.87782584891961
2315	16-17	4.6607039083601585
2315	18-19	0.8134507853257809
2315	20-21	3.000401305870067
2315	22-23	2.254559835419215
2315	24-25	1.1625171703296786
2315	26-27	0.25155340897529044
2315	28-29	1.451914274961137
2315	30-31	3.702417880152243
2315	32-33	6.050966221278735
2315	34-35	0.8694746370527611
2315	36-37	1.9624678995772769
2315	38-39	-0.0674222623441274
2315	40-41	4.584699660871536
2315	42-43	2.077576864686222
2315	44-45	4.006082921707918
2315	46-47	2.712604264948009
2315	48-49	3.692800024050026
2315	50-51	2.0357113372738347
2315	52-53	5.1919082797988985
2315	54-55	5.024255648865026
2315	56-57	2.1073755671411973
2315	58-59	2.7516368758556276
2315	60-61	5.0688477899415325
2315	62-63	4.153592111013985
2315	64-65	5.333292474308095
2315	66-67	5.536026155557412
2315	68-69	3.182605964637215
2315	70-71	2.214161359473856
2315	72-73	-1.7357512712981418
2315	74-75	2.9983259651228416
2315	76-77	1.1083631501600202
2315	78-79	0.7587657857189178
2315	80-81	1.2208232450419878
2315	82-83	1.8663166983479513
2315	84-85	2.5580515982859673
2315	86-87	3.035059515137636
2315	88-89	0.5941215468559164
2315	90-91	-0.02144000386187983
2315	92-93	0.4678808460058512
2315	94-95	1.9398299038923987
2315	96-97	-1.7659148577117332
2315	98-99	1.2354982257325968
2315	100-101	-0.6544493267149534
2316	1	-4.224870181901448
2316	2	-4.337582180550925
2316	3	-3.575011851574356
2316	4	-3.8356128535816083
2316	5	-3.862013420607166
2316	6	-2.741621717402978
2316	7	-6.524697437978695
2316	8	-3.180047094109593
2316	9	-3.508362326331074
2316	10-11	-4.351030147514528
2316	12-13	-5.883489456145707
2316	14-15	-4.91063568954193
2316	16-17	-3.2469883993321496
2316	18-19	-5.751933830058832
2316	20-21	-3.488060232591476
2316	22-23	-4.218517087657705
2316	24-25	-4.883636675824171
2316	26-27	-4.6638312064093235
2316	28-29	-3.2711626481157836
2316	30-31	-5.178351350616989
2316	32-33	-4.568264547952033
2316	34-35	-6.330525362947242
2316	36-37	-4.933685946576571
2316	38-39	-1.19049918542105
2316	40-41	-3.0229926468207715
2316	42-43	-4.183961596852235
2316	44-45	-6.586224770599774
2316	46-47	-4.525857273513534
2316	48-49	-6.695661514411512
2316	50-51	-8.056596355033857
2316	52-53	-8.577322489431872
2316	54-55	-6.025744351134975
2316	56-57	-7.127239817474187
2316	58-59	-8.459901585682834
2316	60-61	-7.404229133135388
2316	62-63	-8.469484812062941
2316	64-65	-6.8359382949226735
2316	66-67	-6.756281536750283
2316	68-69	-7.632778650747403
2316	70-71	-8.39353094821845
2316	72-73	-7.381905117451989
2316	74-75	-6.097827881031005
2316	76-77	-6.826252234455362
2316	78-79	-7.518157291204162
2316	80-81	-6.44456137034263
2316	82-83	-6.875990993959743
2316	84-85	-4.8188714786371065
2316	86-87	-5.507248177170059
2316	88-89	-3.0020322992979303
2316	90-91	-4.125286157708036
2316	92-93	-3.593657615532612
2316	94-95	-2.8024777884152954
2316	96-97	-5.123607165404042
2316	98-99	-4.433732543498172
2316	100-101	-4.792910865176493
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	217.0
8	543.0
9	369.0
10	282.0
11	303.0
12	312.0
13	267.0
14	218.0
15	200.0
16	180.0
17	259.0
18	269.0
19	334.0
20	397.0
21	449.0
22	328.0
23	252.0
24	216.0
25	163.0
26	142.0
27	116.0
28	141.0
29	148.0
30	148.0
31	144.0
32	152.0
33	184.0
34	277.0
35	401.0
36	585.0
37	774.0
38	504.0
39	44.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.429000429000425	21.675246675246676	12.966537966537967	14.929214929214929
2	19.20849420849421	25.868725868725868	33.987558987558984	20.935220935220936
3	23.637923637923638	33.26898326898327	27.938652938652943	15.154440154440154
4	14.124839124839125	23.08022308022308	33.29043329043329	29.504504504504503
5	11.068211068211069	39.37151437151437	29.139854139854137	20.42042042042042
6	23.584298584298587	37.676962676962674	14.993564993564993	23.745173745173744
7	20.345345345345343	41.484341484341485	19.36936936936937	18.800943800943802
8	25.74002574002574	29.933504933504935	18.98326898326898	25.343200343200344
9	28.54997854997855	19.004719004719004	19.176319176319176	33.26898326898327
10-11	24.78013728013728	28.68940368940369	21.235521235521233	25.294937794937795
12-13	30.49120549120549	29.708279708279704	22.324109824109826	17.47640497640498
14-15	26.38888888888889	28.56070356070356	27.627627627627625	17.422779922779924
16-17	17.43886743886744	33.23680823680824	27.547190047190046	21.777134277134277
18-19	18.463105963105964	31.05963105963106	31.263406263406264	19.213856713856714
20-21	23.144573144573144	27.708065208065207	30.64671814671815	18.500643500643502
22-23	20.538395538395537	30.137280137280136	30.357142857142854	18.96718146718147
24-25	21.567996567996566	30.952380952380953	28.791291291291294	18.68833118833119
26-27	20.42042042042042	29.61175461175461	31.32775632775633	18.64006864006864
28-29	19.573144573144575	31.53153153153153	31.006006006006004	17.88931788931789
30-31	21.047833547833548	29.912054912054913	30.947018447018447	18.093093093093092
32-33	19.21921921921922	30.904118404118403	32.45388245388246	17.422779922779924
34-35	18.205705705705704	30.78078078078078	31.53153153153153	19.48198198198198
36-37	18.194980694980696	30.807593307593308	32.0945945945946	18.902831402831403
38-39	19.128056628056626	30.67889317889318	30.753968253968257	19.43908193908194
40-41	18.11990561990562	31.134706134706136	30.829043329043326	19.916344916344915
42-43	18.473830973830975	30.947018447018447	30.79150579150579	19.787644787644787
44-45	17.658730158730158	30.244530244530242	31.279493779493777	20.817245817245816
46-47	17.84105534105534	30.41076791076791	30.68961818961819	21.058558558558556
48-49	17.942942942942945	29.87987987987988	30.244530244530242	21.932646932646932
50-51	17.294079794079796	29.965679965679964	30.60918060918061	22.131059631059628
52-53	17.186829686829686	30.2981552981553	28.5982410982411	23.916773916773916
54-55	17.487129987129986	28.93071643071643	28.72694122694123	24.855212355212355
56-57	18.763406263406264	27.220077220077222	28.909266409266408	25.107250107250106
58-59	20.447232947232948	26.372801372801373	28.657228657228657	24.522737022737022
60-61	19.13341913341913	27.10746460746461	28.925353925353924	24.833762333762333
62-63	18.699056199056198	25.975975975975974	28.94144144144144	26.383526383526384
64-65	18.44165594165594	27.187902187902186	28.86100386100386	25.509438009438007
66-67	18.661518661518663	26.195838695838695	27.654440154440152	27.48820248820249
68-69	18.082368082368085	27.46138996138996	28.330115830115833	26.126126126126124
70-71	19.192406692406692	26.43178893178893	27.595452595452596	26.78035178035178
72-73	18.54890604890605	25.573788073788073	29.429429429429426	26.44787644787645
74-75	17.235092235092235	27.246889746889746	28.812741312741313	26.705276705276702
76-77	18.538181038181037	24.222436722436722	29.252466752466756	27.986915486915485
78-79	19.16023166023166	23.954311454311455	29.096954096954097	27.78850278850279
80-81	20.60810810810811	24.60853710853711	29.000429000429	25.78292578292578
82-83	20.96739596739597	24.115186615186616	28.308665808665808	26.608751608751607
84-85	20.163020163020164	25.15551265551266	27.927927927927925	26.753539253539255
86-87	21.08000858000858	25.16087516087516	26.013513513513516	27.745602745602742
88-89	20.045045045045047	24.125911625911627	26.97876447876448	28.850278850278848
90-91	21.385671385671387	22.704847704847705	26.807164307164307	29.102316602316602
92-93	23.21964821964822	23.251823251823254	26.528314028314025	27.000214500214497
94-95	22.17932217932218	22.442084942084943	27.885027885027885	27.493564993564995
96-97	22.195409695409694	22.726297726297727	27.9011154011154	27.177177177177175
98-99	21.84148434148434	23.428785928785928	26.67846417846418	28.05126555126555
100-101	21.986271986271984	22.86036036036036	27.944015444015445	27.20935220935221
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38.0
1	24.5
2	10.5
3	11.0
4	9.5
5	8.0
6	13.0
7	20.0
8	24.5
9	33.0
10	29.0
11	17.5
12	16.5
13	15.5
14	20.0
15	22.0
16	17.5
17	17.5
18	17.5
19	20.0
20	34.0
21	44.0
22	38.5
23	45.0
24	63.5
25	80.0
26	101.5
27	113.0
28	128.5
29	158.0
30	196.0
31	222.5
32	228.0
33	223.5
34	218.0
35	208.0
36	185.5
37	188.0
38	183.5
39	160.0
40	156.0
41	149.0
42	134.5
43	134.0
44	152.0
45	173.0
46	203.5
47	306.5
48	539.5
49	628.0
50	556.5
51	561.0
52	455.0
53	278.0
54	192.5
55	146.5
56	127.0
57	124.5
58	121.0
59	117.5
60	108.5
61	96.0
62	82.0
63	73.5
64	70.5
65	63.0
66	55.0
67	47.5
68	40.0
69	37.0
70	33.5
71	27.0
72	22.0
73	19.0
74	15.5
75	16.5
76	12.5
77	9.0
78	9.0
79	7.0
80	7.0
81	5.5
82	5.5
83	4.0
84	3.5
85	3.5
86	3.5
87	2.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9324.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.38652938652939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.58254525803837	75.08580008580009
2	2.8235611996757632	4.483054483054483
3	0.8646311807619562	2.0592020592020592
4	0.4728451769791948	1.5015015015015014
5	0.3242366927857336	1.287001287001287
6	0.10807889759524453	0.5148005148005148
7	0.1215887597946501	0.6756756756756757
8	0.027019724398811132	0.1716001716001716
9	0.10807889759524453	0.7722007722007722
>10	0.49986490137800593	7.968682968682969
>50	0.027019724398811132	1.6194766194766195
>100	0.0405295865982167	3.861003861003861
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	133	1.4264264264264264	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	1.2977262977262978	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	106	1.136851136851137	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	85	0.9116259116259117	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66	0.7078507078507079	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41	0.43972543972543976	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	40	0.42900042900042895	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	34	0.36465036465036466	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	34	0.36465036465036466	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA	29	0.31102531102531106	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	28	0.3003003003003003	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	27	0.28957528957528955	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA	27	0.28957528957528955	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	27	0.28957528957528955	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	26	0.27885027885027885	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	23	0.24667524667524665	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	23	0.24667524667524665	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	22	0.23595023595023595	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	21	0.22522522522522523	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21	0.22522522522522523	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	21	0.22522522522522523	No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	21	0.22522522522522523	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	19	0.2037752037752038	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.2037752037752038	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	19	0.2037752037752038	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	18	0.19305019305019305	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.1716001716001716	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	16	0.1716001716001716	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.1716001716001716	No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.16087516087516088	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	15	0.16087516087516088	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.15015015015015015	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	14	0.15015015015015015	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	12	0.1287001287001287	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	12	0.1287001287001287	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC	11	0.11797511797511798	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT	11	0.11797511797511798	No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.11797511797511798	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	10	0.10725010725010724	No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.10725010725010724	No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.10725010725010724	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	10	0.10725010725010724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.010725010725010725	0.0
18-19	0.0	0.0	0.0	0.02145002145002145	0.0
20-21	0.0	0.0	0.0	0.0429000429000429	0.0
22-23	0.0	0.0	0.0	0.16087516087516088	0.0
24-25	0.0	0.0	0.0	0.3861003861003861	0.0
26-27	0.0	0.0	0.0	0.8043758043758044	0.0
28-29	0.0	0.0	0.0	1.5926640926640925	0.0
30-31	0.0	0.0	0.0	2.7616902616902617	0.0
32-33	0.0	0.0	0.0	3.844916344916345	0.0
34-35	0.0	0.0	0.0	5.239167739167739	0.0
36-37	0.0	0.0	0.0	6.885456885456886	0.0
38-39	0.0	0.0	0.0	8.44058344058344	0.0
40-41	0.0	0.0	0.0	11.23981123981124	0.0
42-43	0.0	0.0	0.0	12.8003003003003	0.0
44-45	0.0	0.0	0.0	13.652938652938653	0.0
46-47	0.0	0.0	0.0	14.446589446589446	0.0
48-49	0.0	0.0	0.0	15.100815100815101	0.0
50-51	0.0	0.0	0.0	15.578078078078079	0.0
52-53	0.0	0.0	0.0	15.974903474903474	0.0
54-55	0.0	0.0	0.0	16.457528957528957	0.0
56-57	0.0	0.0	0.0	17.13856713856714	0.0
58-59	0.0	0.0	0.0	17.524667524667525	0.0
60-61	0.0	0.0	0.0	17.937580437580436	0.0
62-63	0.0	0.0	0.0	18.194980694980693	0.0
64-65	0.0	0.0	0.0	18.404118404118407	0.0
66-67	0.0	0.0	0.0	18.661518661518663	0.0
68-69	0.0	0.0	0.0	18.956456456456458	0.0
70-71	0.0	0.0	0.0	19.272844272844274	0.0
72-73	0.0	0.0	0.0	19.535607035607036	0.0
74-75	0.0	0.0	0.0	19.77155727155727	0.0
76-77	0.0	0.0	0.0	20.018232518232516	0.0
78-79	0.0	0.0	0.0	20.318532818532816	0.0
80-81	0.0	0.0	0.0	20.586658086658087	0.0
82-83	0.0	0.0	0.0	20.785070785070786	0.0
84-85	0.0	0.0	0.0	21.004933504933504	0.0
86-87	0.0	0.0	0.0	21.176533676533676	0.0
88-89	0.0	0.0	0.0	21.32132132132132	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCCT	30	0.003852699	31.666668	36-37
CGCCTGT	35	0.008184729	27.142855	38-39
GAGTACT	110	6.366463E-11	25.909092	12-13
ACTTTTT	120	1.891749E-10	23.75	16-17
GGGAAGC	80	1.7014136E-6	23.749998	20-21
GTACTTT	125	3.1650416E-10	22.8	14-15
AGTACTT	115	3.083187E-9	22.71739	12-13
ATGGGAA	85	2.8824325E-6	22.35294	18-19
TACTTTT	120	5.0422386E-9	21.770834	14-15
TGGTCGC	125	8.074494E-9	20.9	94-95
TCGGTGG	140	1.3023964E-9	20.357141	90-91
TCTCGGT	140	1.3023964E-9	20.357141	88-89
GGTGGTC	130	1.2687451E-8	20.096155	92-93
GAAGCAG	95	7.559316E-6	20.0	22-23
GAGTACA	145	2.0172592E-9	19.655172	12-13
GATCTCG	150	3.075911E-9	18.999998	86-87
AGAGTAC	230	0.0	17.554346	10-11
TAGATCT	150	6.554728E-8	17.416666	84-85
TGTAGAT	155	9.534415E-8	16.85484	82-83
TCCTTGT	170	1.4517354E-8	16.764706	76-77
>>END_MODULE