FastQCFastQC Report
Fri 10 Feb 2017
SRR2031845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9324
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT118012.655512655512654No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT6366.82110682110682RNA PCR Primer, Index 46 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT5095.459030459030459RNA PCR Primer, Index 46 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT2672.8635778635778637RNA PCR Primer, Index 46 (96% over 26bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG1882.016302016302016RNA PCR Primer, Index 46 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1601.7160017160017158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1431.5336765336765337No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG1371.4693264693264694TruSeq Adapter, Index 4 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1041.1154011154011154No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC910.975975975975976TruSeq Adapter, Index 18 (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT910.975975975975976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.8043758043758044No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT470.504075504075504No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA440.4719004719004719RNA PCR Primer, Index 46 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.3861003861003861No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC360.3861003861003861No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT330.35392535392535396No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.33247533247533245No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA300.32175032175032175No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT300.32175032175032175No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT280.3003003003003003TruSeq Adapter, Index 4 (96% over 26bp)
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT280.3003003003003003No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT270.28957528957528955No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC260.27885027885027885No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC260.27885027885027885No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC260.27885027885027885No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA260.27885027885027885No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC220.23595023595023595No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC220.23595023595023595No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC220.23595023595023595No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.22522522522522523No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG200.21450021450021448No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2037752037752038No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA190.2037752037752038No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA180.19305019305019305No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.19305019305019305No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT170.18232518232518233No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA170.18232518232518233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.18232518232518233No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC170.18232518232518233No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT150.16087516087516088No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAAGGCG150.16087516087516088No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG140.15015015015015015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC140.15015015015015015No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGC120.1287001287001287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC120.1287001287001287No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1287001287001287No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC120.1287001287001287No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1287001287001287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.11797511797511798No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT100.10725010725010724No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA100.10725010725010724No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC100.10725010725010724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10725010725010724No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG100.10725010725010724No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA100.10725010725010724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3850.035.9705851
TATACAC4000.034.435553
TTATACA4000.034.435552
TACACAT4450.030.9533045
ATACACA4500.030.6093794
GAGTACT652.77636E-729.22911512-13
GCCTGTC350.008186988527.14132138-39
ACATCTC5350.026.6340088
ACACATC5300.025.9890966
CATCTCC5500.025.9076239
CACATCT5350.025.7462087
ATGGGAA857.434284E-522.3516775
GTACTTT852.8838494E-622.35167714-15
GAGTACA1358.276402E-1021.2240241
ACTTTTT904.7355934E-621.10991716-17
GCAGAGT3451.8189894E-1220.6510079
CGCAGAG3551.8189894E-1220.0692888
AGAGTAC1703.274181E-1119.55771610-11
AACGCAG3703.6379788E-1219.2556696
TGAAAAA3700.019.25566958-59
Produced by FastQC (version 0.11.5)