##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031844_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21522 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.558637673078714 34.0 31.0 34.0 31.0 34.0 2 32.7253972679119 34.0 31.0 34.0 31.0 34.0 3 32.75164947495586 34.0 31.0 34.0 31.0 34.0 4 36.15704860143109 37.0 35.0 37.0 35.0 37.0 5 36.17252114115789 37.0 35.0 37.0 35.0 37.0 6 36.28515007898894 37.0 37.0 37.0 35.0 37.0 7 36.23547997398011 37.0 37.0 37.0 35.0 37.0 8 36.27144317442617 37.0 37.0 37.0 35.0 37.0 9 38.00608679490754 39.0 39.0 39.0 35.0 39.0 10-11 38.012034197565285 39.0 39.0 39.0 35.0 39.0 12-13 38.03719449865254 39.0 39.0 39.0 35.0 39.0 14-15 39.44751881795372 41.0 39.0 41.0 36.0 41.0 16-17 39.36172288820741 41.0 39.0 41.0 36.0 41.0 18-19 39.38332868692501 41.0 39.0 41.0 36.0 41.0 20-21 39.29936808846762 41.0 39.0 41.0 36.0 41.0 22-23 39.18378868134932 41.0 39.0 41.0 35.0 41.0 24-25 38.90205371248025 40.5 38.5 41.0 35.0 41.0 26-27 38.569394108354246 40.0 38.0 41.0 34.5 41.0 28-29 38.34636650868878 40.0 38.0 41.0 34.5 41.0 30-31 38.028993587956506 40.0 37.0 41.0 33.5 41.0 32-33 37.363256202955114 39.5 35.5 41.0 33.0 41.0 34-35 37.394619459158065 39.5 36.0 41.0 33.0 41.0 36-37 37.20476721494285 40.0 35.5 41.0 33.0 41.0 38-39 36.49486571879937 39.0 35.0 41.0 30.0 41.0 40-41 36.59090697890531 39.0 35.0 41.0 30.5 41.0 42-43 36.56978905306198 39.5 35.5 41.0 30.0 41.0 44-45 36.14134374128798 39.0 35.0 41.0 28.0 41.0 46-47 35.91664343462503 39.0 35.0 41.0 27.0 41.0 48-49 35.97072762754391 39.5 35.0 41.0 26.5 41.0 50-51 35.36664808103336 39.0 35.0 40.5 23.5 41.0 52-53 35.61337236316328 39.0 35.0 40.5 26.5 41.0 54-55 35.516610909766754 39.0 35.0 41.0 25.5 41.0 56-57 34.60073413251557 38.0 34.5 40.0 22.5 41.0 58-59 33.71624384350896 37.0 33.0 40.0 19.0 41.0 60-61 32.26461295418641 36.5 30.5 39.5 11.5 41.0 62-63 30.93244122293467 35.5 27.0 39.0 8.0 41.0 64-65 29.883468079174797 35.0 24.5 39.0 7.0 40.5 66-67 28.459669175727164 35.0 19.0 37.0 7.0 39.5 68-69 26.872223771025 34.0 13.5 37.0 7.0 39.0 70-71 25.448424867577362 33.0 10.0 36.0 7.0 38.0 72-73 24.385675123129822 32.5 8.0 35.0 7.0 37.0 74-75 24.22061146733575 32.0 9.0 35.0 7.0 37.0 76-77 23.53001579778831 30.0 10.0 34.5 7.0 35.5 78-79 24.144015426075644 31.5 10.0 35.0 7.0 36.0 80-81 24.099061425518073 32.0 10.0 35.0 7.0 35.5 82-83 23.869552086237338 31.5 10.0 35.0 7.0 35.0 84-85 23.69967939782548 31.0 8.0 35.0 7.0 35.0 86-87 23.706556082148502 31.0 8.0 35.0 7.0 35.0 88-89 23.51695939039123 31.0 8.0 35.0 7.0 35.0 90-91 23.450376359074433 31.0 8.0 35.0 7.0 35.0 92-93 23.41283337979742 31.0 8.0 35.0 7.0 35.0 94-95 23.333356565374963 31.0 8.0 35.0 7.0 35.0 96-97 23.26342812006319 31.0 8.0 35.0 7.0 35.0 98-99 23.23836074714246 31.0 8.0 35.0 7.0 35.0 100-101 22.21438528017842 27.5 7.5 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5994661591365471 1101 2 0.5722505730547027 1101 3 0.6561771944948873 1101 4 0.4484634832557646 1101 5 0.8548828922778284 1101 6 0.6939131767060402 1101 7 0.705997301763226 1101 8 0.426123963281853 1101 9 0.6185721431474533 1101 10-11 0.8006925760699986 1101 12-13 0.24473311609284565 1101 14-15 0.23339538247984848 1101 16-17 0.4783338111579525 1101 18-19 1.0576814704415014 1101 20-21 0.5256559143380244 1101 22-23 0.8241542680984182 1101 24-25 0.7162172310543014 1101 26-27 1.0394420679844316 1101 28-29 1.480890970051405 1101 30-31 1.9905201896421403 1101 32-33 1.4280911580734923 1101 34-35 1.4921405550308933 1101 36-37 1.588218728902568 1101 38-39 1.786971502930328 1101 40-41 2.7315212930654056 1101 42-43 2.551252319526462 1101 44-45 2.575463176354411 1101 46-47 0.3941808141884309 1101 48-49 1.2540398569009028 1101 50-51 2.0484653576547984 1101 52-53 1.4509969231974509 1101 54-55 1.7265269161281296 1101 56-57 2.031991240271978 1101 58-59 1.1212999717614096 1101 60-61 1.2842069521078088 1101 62-63 0.8643560998926674 1101 64-65 2.162711045988388 1101 66-67 4.065457510486052 1101 68-69 3.1793491380876517 1101 70-71 1.7022581464307187 1101 72-73 3.319991091967882 1101 74-75 3.4050713526524703 1101 76-77 3.385673562701868 1101 78-79 2.2845883040981683 1101 80-81 2.2055039886430876 1101 82-83 2.9283380171018223 1101 84-85 2.9807619937794954 1101 86-87 4.53452510057441 1101 88-89 3.4958596871192285 1101 90-91 2.3730316875113715 1101 92-93 2.0154196992582847 1101 94-95 1.2567551318508627 1101 96-97 3.570191877712304 1101 98-99 3.1654163491993828 1101 100-101 1.214997415643115 1102 1 0.39946615913654426 1102 2 0.41538782795666407 1102 3 0.7267654297890047 1102 4 0.3778752479616472 1102 5 0.5019417158072415 1102 6 0.6350896472942722 1102 7 0.5295267135279289 1102 8 0.6927906299485187 1102 9 0.7166113588337311 1102 10-11 0.6104964976386213 1102 12-13 0.5623801749163775 1102 14-15 0.5118267550288635 1102 16-17 0.6744122425305008 1102 18-19 0.5400344116179738 1102 20-21 0.9060480712007646 1102 22-23 0.22807583672587128 1102 24-25 0.527981936936655 1102 26-27 0.6237557934746292 1102 28-29 1.004420381816118 1102 30-31 -0.1271268691813887 1102 32-33 0.988875471798984 1102 34-35 -0.04315356261616188 1102 36-37 0.9980226504711922 1102 38-39 -0.7659696735402619 1102 40-41 -1.0567140010522493 1102 42-43 -1.4526692491009854 1102 44-45 -0.8225760393318637 1102 46-47 -0.86464271522334 1102 48-49 -0.8165483783932075 1102 50-51 0.5092496713802959 1102 52-53 0.8666831977072533 1102 54-55 -0.1538652407346106 1102 56-57 -1.1993813087476326 1102 58-59 -0.4787000282385918 1102 60-61 -0.6412832439706229 1102 62-63 0.18004237440247195 1102 64-65 1.1901620263805484 1102 66-67 1.8458496673487979 1102 68-69 -0.8343763521084284 1102 70-71 1.0081404993718976 1102 72-73 0.3964616802031742 1102 74-75 -0.2341443336220408 1102 76-77 0.8543010136822602 1102 78-79 0.841451049196209 1102 80-81 1.4917784984470082 1102 82-83 -0.3108776691726831 1102 84-85 0.9886051310343973 1102 86-87 0.454132943711663 1102 88-89 0.5919381184917789 1102 90-91 1.6651885502564703 1102 92-93 0.9291451894543634 1102 94-95 1.4077355240077232 1102 96-97 -0.18863165169946328 1102 98-99 0.7928673295915445 1102 100-101 0.7640170234862502 1103 1 0.17724393691432283 1103 2 0.23891723972136703 1103 3 -0.4660450277273327 1103 4 0.07068570547798458 1103 5 0.07710511450004987 1103 6 -0.028309045516181186 1103 7 0.15044174620766881 1103 8 0.41501285217074013 1103 9 0.6741276987030105 1103 10-11 0.2673592427366671 1103 12-13 0.1947331160928485 1103 14-15 0.23895093803540135 1103 16-17 -0.4661106332864975 1103 18-19 0.6299036926637243 1103 20-21 -0.3521218634397556 1103 22-23 0.06304315698731244 1103 24-25 -0.3560049911679215 1103 26-27 0.17277540131777158 1103 28-29 -0.04133125217081357 1103 30-31 0.07385352297546888 1103 32-33 -0.08301995303761345 1103 34-35 0.24769611058645324 1103 36-37 -0.1228923822085406 1103 38-39 -1.968584052625232 1103 40-41 -2.551812040267926 1103 42-43 -0.7876365693624265 1103 44-45 -0.5300923792011432 1103 46-47 -0.9447080747004577 1103 48-49 0.7095954124564656 1103 50-51 0.6873542465436984 1103 52-53 0.23433025653078232 1103 54-55 0.326526916128131 1103 56-57 0.2597690180497523 1103 58-59 -2.0620333615719275 1103 60-61 -2.0269041590033012 1103 62-63 -2.6745327889962205 1103 64-65 -4.631733398456056 1103 66-67 -6.1900980450695045 1103 68-69 -7.10398419524568 1103 70-71 -5.392186298013723 1103 72-73 -6.224453352476562 1103 74-75 -5.417150869569749 1103 76-77 -6.547659770631462 1103 78-79 -7.315411695901833 1103 80-81 -7.611162678023579 1103 82-83 -5.71610642734262 1103 84-85 -6.647015783998281 1103 86-87 -6.259919343870038 1103 88-89 -5.270806979547441 1103 90-91 -5.838079423599737 1103 92-93 -5.773469189630607 1103 94-95 -4.154355979260249 1103 96-97 -5.879808122287699 1103 98-99 -6.612361428578392 1103 100-101 -6.50722480657911 1104 1 1.2818191003130153 1104 2 0.944799592662541 1104 3 0.5502948415537148 1104 4 0.6131693656087052 1104 5 0.7372358334542994 1104 6 0.6350896472942722 1104 7 0.588350242939697 1104 8 0.5751435711249897 1104 9 0.657787829421963 1104 10-11 0.6987317917562734 1104 12-13 0.8859095866810804 1104 14-15 1.1882973432641606 1104 16-17 0.9685298895893268 1104 18-19 1.2459167645591478 1104 20-21 0.9648716006125326 1104 22-23 1.2868993661376393 1104 24-25 1.233864289877829 1104 26-27 1.8590499111216943 1104 28-29 2.180890970051408 1104 30-31 2.5493437190539083 1104 32-33 2.312404883563694 1104 34-35 1.7803758491485482 1104 36-37 2.0568461798829603 1104 38-39 3.175206797047977 1104 40-41 3.3844624695359897 1104 42-43 3.6355660450166596 1104 44-45 2.9715416077269623 1104 46-47 2.841239637717841 1104 48-49 3.3305104451361984 1104 50-51 4.332779083144999 1104 52-53 3.9255067271190143 1104 54-55 3.6696641710300852 1104 56-57 4.212383397134715 1104 58-59 4.550711736467292 1104 60-61 4.770481461911729 1104 62-63 4.503571786167175 1104 64-65 4.866632614615838 1104 66-67 4.11055554970174 1104 68-69 5.547976589068043 1104 70-71 4.566964028783662 1104 72-73 4.602344033144352 1104 74-75 4.265855666377963 1104 76-77 4.207242190152851 1104 78-79 3.3414510491962055 1104 80-81 2.991778498447008 1104 82-83 3.600887036709665 1104 84-85 3.5180168957402778 1104 86-87 3.4247211790057825 1104 88-89 3.944879294962366 1104 90-91 3.253423844374119 1104 92-93 3.6938510718073054 1104 94-95 3.26067670047831 1104 96-97 4.664309524771127 1104 98-99 4.8811026237091895 1104 100-101 4.852252317603895 1105 1 0.08367668545233187 1105 2 0.028390923931894463 1105 3 0.20354561554751882 1105 4 -0.15153651674423685 1105 5 0.4338302606988833 1105 6 0.06233422933762256 1105 7 -0.18873954034203422 1105 8 -0.6756304226830636 1105 9 -0.5744103129928959 1105 10-11 -0.2501846169124562 1105 12-13 -0.4236879365387267 1105 14-15 0.0474304701991457 1105 16-17 -0.14447320638590355 1105 18-19 -0.6317922137690246 1105 20-21 -0.7936423312760112 1105 22-23 -0.5319860827787721 1105 24-25 -0.19957224262990536 1105 26-27 -0.23073337061205734 1105 28-29 0.12825939110403795 1105 30-31 -0.9305324419368048 1105 32-33 -0.778926385786157 1105 34-35 -0.8604910239164738 1105 36-37 -1.1784479377640977 1105 38-39 -0.01829165496441476 1105 40-41 0.6894160299075125 1105 42-43 0.4389716177720757 1105 44-45 1.100024579863181 1105 46-47 0.29944397208316786 1105 48-49 0.6540398569009085 1105 50-51 -2.0480258704153727 1105 52-53 -1.0946171118902726 1105 54-55 -0.29628010141573213 1105 56-57 0.14427194202636429 1105 58-59 -0.16291055455438652 1105 60-61 0.4438560749148266 1105 62-63 1.5345315384891585 1105 64-65 1.764465431953301 1105 66-67 1.9356329490825424 1105 68-69 1.128471945105197 1105 70-71 0.24962656748335021 1105 72-73 -0.2273773290847494 1105 74-75 0.6822643351086093 1105 76-77 1.4084805802457296 1105 78-79 1.5968690058525574 1105 80-81 1.7704162693448424 1105 82-83 1.368688894294806 1105 84-85 2.0737444499198467 1105 86-87 1.574875977767391 1105 88-89 0.5362105643122099 1105 90-91 1.9484702840026031 1105 92-93 1.518928471188108 1105 94-95 -0.20991153481580582 1105 96-97 1.0280866145544074 1105 98-99 1.4461181035853485 1105 100-101 2.1448219770466217 1106 1 0.6626240538733867 1106 2 0.5547067134055794 1106 3 0.7298614050212038 1106 4 0.21688453588734546 1106 5 0.4338302606988833 1106 6 0.4833868609165677 1106 7 0.33757624913164364 1106 8 0.5875274720537789 1106 9 0.7413791606913165 1106 10-11 0.7761311725612217 1106 12-13 0.7605225897770538 1106 14-15 0.8895357333570431 1106 16-17 1.1976320567719867 1106 18-19 1.105049891494133 1106 20-21 1.2063576687239888 1106 22-23 0.7838033909054403 1106 24-25 0.9320067047385052 1106 26-27 1.137687682019525 1106 28-29 0.8914172858408804 1106 30-31 1.2273622949052978 1106 32-33 1.3263367721085828 1106 34-35 1.797403712925636 1106 36-37 2.1899731148674775 1106 38-39 0.11328729240401003 1106 40-41 1.873626556223293 1106 42-43 0.5968663546141855 1106 44-45 -0.47892278855786685 1106 46-47 0.8257597615568528 1106 48-49 0.8382503832166961 1106 50-51 0.8203951822162097 1106 52-53 0.5632776249518372 1106 54-55 0.5721409512158502 1106 56-57 0.6705877315000492 1106 58-59 0.9423526033403533 1106 60-61 0.9175402854411416 1106 62-63 0.7187420648049461 1106 64-65 1.6065706951111949 1106 66-67 2.3040540017141247 1106 68-69 2.3916298398420395 1106 70-71 2.74962656748335 1106 72-73 3.851570039336302 1106 74-75 4.603316966687554 1106 76-77 3.8295332118246748 1106 78-79 3.0968690058525574 1106 80-81 2.9809425851343185 1106 82-83 3.237109946926388 1106 84-85 3.626376028867213 1106 86-87 3.574875977767391 1106 88-89 3.1414737222069498 1106 90-91 3.4747860734762845 1106 92-93 4.597875839609159 1106 94-95 4.237456886236824 1106 96-97 4.396507667185986 1106 98-99 5.551381261480088 1106 100-101 4.118506187572937 1107 1 0.04946615913654284 1107 2 0.08891723972136845 1107 3 -0.14382280550510984 1107 4 0.24846348325576173 1107 5 0.35488289227782843 1107 6 0.1439131767060431 1107 7 0.1559973017632288 1107 8 0.14279062994852154 1107 9 0.0019054764807862057 1107 10-11 -0.34097409059666717 1107 12-13 0.5197331160928513 1107 14-15 0.5750620491465099 1107 16-17 0.29500047782462246 1107 18-19 0.8576814704415057 1107 20-21 0.6339892476713587 1107 22-23 0.5824876014317582 1107 24-25 0.6412172310542985 1107 26-27 0.9311087346510973 1107 28-29 0.2808909700514093 1107 30-31 0.6655201896421374 1107 32-33 0.7947578247401523 1107 34-35 0.767140555030899 1107 36-37 1.1215520622358994 1107 38-39 1.4369715029303194 1107 40-41 0.5065212930653971 1107 42-43 0.2929189861931363 1107 44-45 1.3421298430210769 1107 46-47 0.7941808141884223 1107 48-49 0.1290398569009099 1107 50-51 -0.43486797567853586 1107 52-53 -1.4406697434692148 1107 54-55 -2.431806417205202 1107 56-57 -1.5596754263946906 1107 58-59 -1.3287000282385932 1107 60-61 0.06754028544114021 1107 62-63 -1.0523105667740005 1107 64-65 -1.453955620678279 1107 66-67 -0.7012091561806137 1107 68-69 -2.0623175285790154 1107 70-71 -2.69774185356928 1107 72-73 -0.7300089080321222 1107 74-75 -3.4032619806808633 1107 76-77 -2.3309931039648006 1107 78-79 -3.157078362568498 1107 80-81 -3.2611626780235774 1107 82-83 -3.6299953162315077 1107 84-85 -3.652571339553841 1107 86-87 -3.6238082327589254 1107 88-89 -4.762473646214104 1107 90-91 -3.5936349791552935 1107 92-93 -1.8429136340750496 1107 94-95 -3.784911534815805 1107 96-97 -3.554808122287696 1107 98-99 -3.6762503174672823 1107 100-101 -3.2683359176902194 1108 1 -0.3831425365156278 1108 2 -0.02195232549602366 1108 3 0.16922067275575614 1108 4 -0.8471886906572834 1108 5 -0.23207362946130417 1108 6 -0.21913030155482716 1108 7 0.18425817132844458 1108 8 0.12757323864416747 1108 9 0.4736446069155704 1108 10-11 0.1079389528815895 1108 12-13 0.07516789870154383 1108 14-15 -0.378198820418703 1108 16-17 -0.007173435218859936 1108 18-19 0.02724668783280748 1108 20-21 -0.19753249145907859 1108 22-23 0.14879194925784844 1108 24-25 0.44230418757603474 1108 26-27 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16-17 0.2908338111579454 2311 18-19 0.032681470441502825 2311 20-21 -0.09517741899531273 2311 22-23 -0.1800123985682447 2311 24-25 -0.09211610227902867 2311 26-27 -1.2855579320155712 2311 28-29 -0.6107756966152635 2311 30-31 0.13635352297546888 2311 32-33 -0.20107550859317058 2311 34-35 -1.620359444969104 2311 36-37 -1.8034479377640977 2311 38-39 -0.01719516373634633 2311 40-41 0.010687959732074148 2311 42-43 0.1637523195264592 2311 44-45 -0.24537015697892173 2311 46-47 -0.8266525191449077 2311 48-49 -0.07512680976576291 2311 50-51 0.6317986909881341 2311 52-53 -0.7240030768025463 2311 54-55 -0.4026397505385404 2311 56-57 -1.5388420930613549 2311 58-59 -1.9995333615719275 2311 60-61 -3.915793047892194 2311 62-63 -4.681477233440667 2311 64-65 -3.9581222873449455 2311 66-67 -3.2178758228472795 2311 68-69 -3.1248175285790154 2311 70-71 -1.6352418535692799 2311 72-73 -1.113342241365455 2311 74-75 -2.7782619806808633 2311 76-77 -2.255993103964798 2311 78-79 -2.1070783625684975 2311 80-81 -2.4444960113569145 2311 82-83 -2.979995316231509 2311 84-85 -1.3275713395538382 2311 86-87 -1.2946415660922597 2311 88-89 -1.4999736462141051 2311 90-91 -2.0603016458219585 2311 92-93 -2.6554136340750496 2311 94-95 -2.855744868149138 2311 96-97 -3.629808122287699 2311 98-99 -3.3970836508006172 2311 100-101 -4.347502584356889 2312 1 0.014850774521157462 2312 2 -1.3380058372017096 2312 3 -0.6668997285820311 2312 4 0.0023096371019164508 2312 5 0.23949827689321523 2312 6 0.30929779209065345 2312 7 0.39830499407091935 2312 8 0.3850983222562121 2312 9 -0.20194067736536425 2312 10-11 0.2994105247879446 2312 12-13 0.425502346862082 2312 14-15 0.6577543568388222 2312 16-17 0.5296158624400036 2312 18-19 0.6384507012107363 2312 20-21 0.8359123245944389 2312 22-23 0.4497952937394487 2312 24-25 -1.5568596920226199 2312 26-27 -0.4169681884258196 2312 28-29 0.08473712389756116 2312 30-31 0.4135971127190601 2312 32-33 0.483219363201691 2312 34-35 0.8459867088770494 2312 36-37 0.8792443699282089 2312 38-39 0.7792791952380185 2312 40-41 0.608444369988483 2312 42-43 1.9794574477315905 2312 44-45 0.24982215071338487 2312 46-47 -0.30581918581157197 2312 48-49 -0.7113447584837047 2312 50-51 0.31128587047531653 2312 52-53 0.2727917949923224 2312 54-55 -0.006806417205204696 2312 56-57 0.007632265913002811 2312 58-59 0.3097615102229412 2312 60-61 -1.0632289453280919 2312 62-63 -0.9907721052355392 2312 64-65 0.492198225475569 2312 66-67 0.12186776689630818 2312 68-69 -0.7834713747328621 2312 70-71 0.30225814643072013 2312 72-73 0.008452630429420793 2312 74-75 -0.3167235191424034 2312 76-77 0.4651607421890489 2312 78-79 -0.5782322087223442 2312 80-81 -0.38039344725434887 2312 82-83 0.4142354529992609 2312 84-85 0.8358901989077019 2312 86-87 1.1781148441641527 2312 88-89 0.600987892247435 2312 90-91 1.1640573285370124 2312 92-93 1.1090094428480306 2312 94-95 0.11701154210727083 2312 96-97 0.08173033925076112 2312 98-99 0.24874968253271845 2312 100-101 0.5739717746174726 2313 1 -1.9453614270703525 2313 2 -0.7266000016579426 2313 3 -0.5714090124016664 2313 4 -0.8411916891580304 2313 5 -0.7658067628945844 2313 6 -0.23712130605257897 2313 7 -0.25951993961608366 2313 8 -0.20376109418941013 2313 9 0.4346640971704474 2313 10-11 -1.2383878837001134 2313 12-13 -1.2794048149416284 2313 14-15 -0.693041399129342 2313 16-17 -0.44120641872711275 2313 18-19 0.0025090566483996213 2313 20-21 -0.05135557991484774 2313 22-23 -1.2080296399475543 2313 24-25 -0.1596448379112232 2313 26-27 -0.028374023969590212 2313 28-29 -0.3880745471899729 2313 30-31 -0.14741084484062128 2313 32-33 -0.06386286491501636 2313 34-35 0.6921405550308961 2313 36-37 0.26982792430487024 2313 38-39 0.7593852960337699 2313 40-41 1.3935902585826412 2313 42-43 1.0696431241241626 2313 44-45 0.9248884637107295 2313 46-47 -0.9782329789150168 2313 48-49 0.6367984775905597 2313 50-51 1.9220285760456122 2313 52-53 1.5877785323928464 2313 54-55 0.11388323796720812 2313 56-57 -0.41743404708434184 2313 58-59 -0.3924931316868623 2313 60-61 -0.9445286800761004 2313 62-63 -2.4954140150498603 2313 64-65 -2.397059068954139 2313 66-67 -3.301209156180615 2313 68-69 -1.7218002871997058 2313 70-71 -1.077052198396867 2313 72-73 -1.9006985632045321 2313 74-75 -1.2006757737843117 2313 76-77 -0.9672000005165202 2313 78-79 0.3110250857073673 2313 80-81 1.0009062874936632 2313 82-83 0.05017709756159405 2313 84-85 0.8577734880323682 2313 86-87 1.1701572844824568 2313 88-89 1.5970091124065853 2313 90-91 0.8822270898102218 2313 92-93 -0.5972239789026368 2313 94-95 0.15215743070143262 2313 96-97 0.990881532884714 2313 98-99 -0.67366411057073 2313 100-101 0.17476753058564043 2314 1 -1.1560893964190129 2314 2 -0.5388605380564115 2314 3 -1.7438228055051113 2314 4 -1.0404254056331226 2314 5 -0.7006726632777287 2314 6 -0.34312386033099784 2314 7 -2.5902989945330717 2314 8 -1.3442464070885194 2314 9 -2.4184648938895847 2314 10-11 -1.325233349855921 2314 12-13 -1.4811928098330753 2314 14-15 -1.3073453582608892 2314 16-17 -2.4290735962494594 2314 18-19 -1.8793555665955353 2314 20-21 -1.9632329745508628 2314 22-23 -1.5202901763460162 2314 24-25 -1.4763753615382953 2314 26-27 -0.6420394134970522 2314 28-29 -1.8191090299485921 2314 30-31 -2.4909612918393407 2314 32-33 -2.1756125456302087 2314 34-35 -0.6597112968209515 2314 36-37 -1.2154849748011358 2314 38-39 -3.3204359044770797 2314 40-41 -1.9499601884160782 2314 42-43 -0.815414347140198 2314 44-45 -0.9097220088307765 2314 46-47 -0.7502636302560148 2314 48-49 -0.10521940235835103 2314 50-51 0.5484653576547984 2314 52-53 0.3917376639381871 2314 54-55 0.882082471683681 2314 56-57 0.38013938842011896 2314 58-59 0.8546333050947439 2314 60-61 0.1582810261818821 2314 62-63 0.6680598035963712 2314 64-65 -0.06691858364123959 2314 66-67 -0.727135082106539 2314 68-69 0.36823802697654173 2314 70-71 0.6911470353196094 2314 72-73 1.886657758634545 2314 74-75 -0.7690027214216038 2314 76-77 -0.2698819928536835 2314 78-79 0.31421793372780016 2314 80-81 0.37957806271716166 2314 82-83 1.3024120911758992 2314 84-85 2.0289101419276427 2314 86-87 2.082673248722557 2314 88-89 2.7477115389710782 2314 90-91 1.5693279838076677 2314 92-93 0.8931974770360611 2314 94-95 1.2715699466656751 2314 96-97 2.685006692527118 2314 98-99 2.276527460310497 2314 100-101 1.807590008235703 2315 1 -0.8908564215086159 2315 2 -1.373985986085085 2315 3 -0.9373711926018871 2315 4 -0.18379458126036496 2315 5 -1.0806009786899153 2315 6 -0.6609255329713761 2315 7 -0.8746478595270943 2315 8 -0.2749513055353532 2315 9 -0.43519129771276255 2315 10-11 -1.2917805422095725 2315 12-13 -2.3455894645523117 2315 14-15 -1.9805831121438047 2315 16-17 -0.8711285544334473 2315 18-19 -0.4068346585907534 2315 20-21 -0.24101075232864133 2315 22-23 -0.09735110824566817 2315 24-25 0.29363658589301167 2315 26-27 0.24804421852206815 2315 28-29 0.22927806682560004 2315 30-31 0.7647137380292364 2315 32-33 1.0173384699014463 2315 34-35 0.9824631356760563 2315 36-37 1.1925198041713898 2315 38-39 1.2160037609948375 2315 40-41 2.4186180672589543 2315 42-43 0.9856609216770025 2315 44-45 1.5389040365694626 2315 46-47 2.5490195238658515 2315 48-49 0.5411366310944601 2315 50-51 0.5377126694827652 2315 52-53 1.0165883210469104 2315 54-55 1.5254516473109234 2315 56-57 1.1943568316698219 2315 58-59 0.21484835885817688 2315 60-61 1.3852822209250135 2315 62-63 2.023495884838905 2315 64-65 3.2508830889991387 2315 66-67 4.166532779303257 2315 68-69 4.603005052066145 2315 70-71 4.770000081914592 2315 72-73 4.590958833903365 2315 74-75 4.003996083835265 2315 76-77 4.15126496055133 2315 78-79 4.050179701947631 2315 80-81 3.332385709073197 2315 82-83 3.782101457962039 2315 84-85 1.3216222088332579 2315 86-87 3.6818369285314 2315 88-89 3.707687644108475 2315 90-91 2.654752117618898 2315 92-93 2.4917637852797903 2315 94-95 2.98363685228097 2315 96-97 3.450837039002625 2315 98-99 4.1277819405972345 2315 100-101 2.252631824245263 2316 1 -0.03803384086345574 2316 2 -0.292332760278633 2316 3 0.03742719449488874 2316 4 0.004713483255763151 2316 5 -0.7701171077221716 2316 6 -0.05608682329395975 2316 7 -0.13775269823677405 2316 8 -0.1197093700514813 2316 9 -0.673094523519211 2316 10-11 0.12152590940333141 2316 12-13 -0.02401688390715151 2316 14-15 -0.22806295085348438 2316 16-17 -0.4331245221753832 2316 18-19 0.07955647044150282 2316 20-21 0.32148924767135867 2316 22-23 -0.3362623985682447 2316 24-25 0.10059223105429993 2316 26-27 -0.6813912653488998 2316 28-29 -0.47535902994859214 2316 30-31 -0.04072981035785972 2316 32-33 -0.836492175259842 2316 34-35 0.08276555503089611 2316 36-37 0.25905206223590227 2316 38-39 -0.20990349706967493 2316 40-41 -0.37472870693459726 2316 42-43 -0.4039560138068694 2316 44-45 0.16087984302107827 2316 46-47 -1.758944185811572 2316 48-49 -1.1115851430990915 2316 50-51 -0.47236797567853017 2316 52-53 -1.7344197434692177 2316 54-55 -2.3349314172052047 2316 56-57 -2.8253004263946906 2316 58-59 -1.6349500282385918 2316 60-61 -4.691834714558858 2316 62-63 -4.671060566773999 2316 64-65 -4.197705620678278 2316 66-67 -3.066834156180615 2316 68-69 -2.3279425285790154 2316 70-71 -0.33836685356927987 2316 72-73 -0.2175089080321193 2316 74-75 0.11236301931913673 2316 76-77 0.1971318960352022 2316 78-79 -1.4195783625684975 2316 80-81 -1.2049126780235788 2316 82-83 -0.24562031623150915 2316 84-85 -0.8744463395538382 2316 86-87 -1.164433232758924 2316 88-89 -0.24997364621410512 2316 90-91 -1.2842599791552942 2316 92-93 -0.7179136340750496 2316 94-95 -1.2567865348158058 2316 96-97 -0.19230812228769878 2316 98-99 0.07687468253271845 2316 100-101 -1.0558359176902208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 5.0 15 34.0 16 80.0 17 199.0 18 256.0 19 267.0 20 319.0 21 331.0 22 319.0 23 324.0 24 450.0 25 800.0 26 1439.0 27 1380.0 28 1573.0 29 1527.0 30 560.0 31 317.0 32 298.0 33 377.0 34 477.0 35 920.0 36 1679.0 37 3914.0 38 3481.0 39 193.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.246511627906976 19.91627906976744 15.093023255813954 28.744186046511626 2 11.18855124988384 19.74258897871945 40.50274138091255 28.566118390484156 3 18.697147105287613 26.91199702629867 40.948796580243474 13.442059288170244 4 7.383142830591953 26.117461202490478 27.520676517052316 38.97871944986526 5 8.298485270885605 36.005018120992474 38.63023882538798 17.066257782733945 6 22.81386488244587 37.92863116810705 14.770932069510268 24.486571879936808 7 15.25880494377846 35.16401821392064 17.688876498466684 31.888300343834214 8 23.283152123408605 29.263079639438715 24.356472446798623 23.097295790354057 9 14.83598178607936 15.556175076665738 18.43230183068488 51.17554130657002 10-11 20.225815444661276 23.74779295604498 25.768980578013196 30.25741102128055 12-13 25.703930861444103 21.522163367716754 24.191524951212713 28.58238081962643 14-15 22.96487315305269 24.31233156769817 21.719635721587213 31.00315955766193 16-17 22.672149428491775 33.83282222841743 20.43025741102128 23.06477093206951 18-19 25.515751324226372 25.74807174054456 21.431558405352664 27.304618529876407 20-21 21.710342904934485 24.67475141715454 21.33166062633584 32.28324505157513 22-23 19.937738128426727 33.869993495028346 21.745190967382214 24.447077409162716 24-25 32.70374500511105 29.899637580150547 21.020351268469472 16.376266146268932 26-27 27.522999721215502 32.239104172474676 26.491497072762755 13.74639903354707 28-29 24.642226558869993 26.867856147198214 24.916364650125452 23.573552643806337 30-31 19.73097295790354 28.503391878078244 30.826596041260107 20.939039122758107 32-33 25.234643620481368 29.985596134188274 29.75792212619645 15.021838119133909 34-35 29.660347551342813 22.181953350060404 29.809032617786453 18.348666480810333 36-37 18.77148963850943 30.12963479230555 27.643806337700955 23.455069231484064 38-39 20.71601152309265 20.78338444382492 31.553758944336032 26.9468450887464 40-41 16.341418083821207 20.562680048322648 36.12814794164111 26.96775392621504 42-43 21.290337569407335 18.521013869851082 36.15454313128732 24.034105429454268 44-45 16.285661183904843 21.735898150729486 35.90512034197565 26.07332032339002 46-47 25.94554409441502 17.465848898801227 28.42207973236688 28.166527274416875 48-49 21.652765838812353 16.727458587923707 33.18309597379365 28.4366795994703 50-51 15.906978905306199 22.497909116253137 36.67642412415203 24.918687854288635 52-53 15.714152959762103 27.653099154353683 37.02490474862931 19.607843137254903 54-55 13.579128333797975 26.45200260198866 31.602546231762847 28.366322832450514 56-57 22.0030666294954 30.82427283709692 21.907815258804945 25.26484527460273 58-59 16.601616950097576 35.20351268469473 31.521234086051482 16.673636279156213 60-61 20.555710435833102 43.4415946473376 20.51853916922219 15.484155747607101 62-63 13.251556546789331 49.72353870458136 18.88300343834216 18.14190131028715 64-65 12.851965430722052 50.51575132422638 17.56806988198123 19.064213363070344 66-67 14.73376080289936 46.422265588699936 16.299600408883933 22.544373199516773 68-69 14.827033431684594 43.809678693399626 19.266779731896012 22.096508143019772 70-71 19.282594554409442 41.24616671313075 17.52160579871759 21.949632933742215 72-73 18.504321159743515 35.94461481274974 22.706997490939504 22.844066536567233 74-75 18.146547718613512 38.54892667967661 20.400055756899917 22.904469844809963 76-77 18.864417805036705 35.49391320509246 20.585912089954466 25.055756899916364 78-79 20.379146919431278 35.152402193104734 21.72660533407676 22.741845553387233 80-81 22.172660533407676 33.15212340860515 20.76479881051947 23.91041724746771 82-83 20.07945358238082 32.36688040144968 24.409906142551808 23.143759873617693 84-85 20.72995074807174 32.006783756156494 22.955580336399965 24.307685159371808 86-87 21.310751788867204 32.3134467056965 22.54204999535359 23.833751510082706 88-89 20.511569556732645 31.61416225257876 21.436204813679026 26.438063377009573 90-91 21.28984295139857 28.902982994145525 23.199516773534057 26.60765728092185 92-93 22.744168757550415 29.748629309543723 23.369110677446333 24.13809125545953 94-95 21.694080475792212 28.477836632283243 24.37970448843044 25.448378403494097 96-97 18.213920639345783 28.954093485735527 27.701886441780506 25.130099433138188 98-99 20.397732552736734 30.001858563330547 23.808196264287705 25.792212619645017 100-101 19.4289564166899 28.54520955301552 25.543629774184556 26.482204256110027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 132.0 1 77.5 2 23.0 3 31.0 4 25.5 5 10.5 6 17.0 7 34.0 8 51.5 9 56.5 10 35.5 11 18.5 12 19.0 13 22.0 14 22.5 15 21.0 16 24.0 17 25.0 18 25.5 19 24.0 20 22.5 21 28.0 22 34.0 23 44.0 24 46.0 25 49.0 26 62.5 27 70.0 28 78.0 29 86.0 30 92.0 31 114.0 32 158.5 33 200.5 34 251.0 35 334.0 36 457.5 37 588.0 38 635.5 39 698.0 40 764.0 41 778.5 42 815.0 43 771.0 44 682.5 45 636.5 46 672.5 47 1121.5 48 2144.5 49 2236.0 50 1546.0 51 1223.5 52 798.5 53 449.0 54 322.5 55 264.0 56 232.0 57 208.5 58 181.0 59 167.5 60 145.5 61 123.5 62 97.0 63 71.0 64 60.5 65 62.5 66 51.0 67 28.5 68 24.0 69 19.0 70 16.0 71 16.0 72 16.5 73 15.5 74 10.5 75 10.0 76 13.0 77 11.0 78 10.5 79 9.0 80 7.5 81 5.0 82 1.5 83 1.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10222098318000186 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0023232041631818605 44-45 0.0 46-47 0.0 48-49 0.0023232041631818605 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0023232041631818605 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 21522.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.74017284638974 #Duplication Level Percentage of deduplicated Percentage of total 1 82.64430577223088 29.53721773069417 2 10.413416536661467 7.443546138834681 3 2.8211128445137805 3.0248118204627823 4 1.2220488819552782 1.747049530712759 5 0.7280291211648465 1.300994331381842 6 0.4030161206448258 0.8642319487036522 7 0.20800832033281333 0.5203977325527367 8 0.22100884035361415 0.631911532385466 9 0.1170046801872075 0.3763590744354614 >10 0.7540301612064483 5.366601616950097 >50 0.13000520020800832 3.03410463711551 >100 0.26001040041601664 16.006876684323018 >500 0.05200208008320333 13.228324505157513 >1k 0.026001040041601666 16.91757271629031 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2474 11.495214199423845 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 1167 5.4223585168664625 TruSeq Adapter, Index 3 (96% over 28bp) ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 877 4.074900102220983 TruSeq Adapter, Index 3 (95% over 21bp) ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 859 3.9912647523464364 RNA PCR Primer, Index 3 (95% over 23bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 571 2.6530991543536846 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 540 2.509060496236409 No Hit CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA 400 1.8585633305454885 RNA PCR Primer, Index 3 (96% over 28bp) CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT 342 1.5890716476163926 TruSeq Adapter, Index 3 (96% over 26bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 302 1.4032153145618438 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 193 0.896756806988198 No Hit CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG 189 0.8781711736827432 TruSeq Adapter, Index 3 (95% over 24bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 188 0.8735247653563795 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 172 0.7991822321345601 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 153 0.7109004739336493 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 146 0.6783756156491032 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 141 0.6551435740172846 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 135 0.6272651240591023 No Hit ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC 131 0.6086794907536475 Illumina PCR Primer Index 12 (95% over 24bp) GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 129 0.59938667410092 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 126 0.5854474491218289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.5854474491218289 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 119 0.5529225908372828 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 117 0.5436297741845554 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 0.5250441408791005 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 112 0.5203977325527367 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.5157513242263729 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 92 0.42746956602546227 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.3856518910881888 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 72 0.3345413994981879 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 71 0.3298949911718242 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 63 0.29272372456091444 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC 60 0.2787844995818232 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.26019886627636835 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 54 0.25090604962364094 No Hit TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC 51 0.23696682464454977 No Hit TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT 51 0.23696682464454977 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 46 0.21373478301273113 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42 0.1951491497072763 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.18120992472818512 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 39 0.18120992472818512 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 38 0.17656351640182139 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 38 0.17656351640182139 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT 37 0.17191710807545765 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 36 0.16727069974909395 No Hit GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG 34 0.1579778830963665 No Hit ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT 34 0.1579778830963665 TruSeq Adapter, Index 3 (96% over 27bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 34 0.1579778830963665 No Hit CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG 30 0.1393922497909116 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.13009943313818417 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 26 0.12080661648545674 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 25 0.11616020815909303 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 24 0.1115137998327293 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 24 0.1115137998327293 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 23 0.10686739150636557 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.10222098318000186 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 22 0.10222098318000186 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT 22 0.10222098318000186 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT 22 0.10222098318000186 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.020908837468636744 0.0 22-23 0.0 0.0 0.0 0.06969612489545582 0.0 24-25 0.0 0.0 0.0 0.19514914970727626 0.0 26-27 0.0 0.0 0.0 0.42049995353591674 0.0 28-29 0.0 0.0 0.0 0.8758479695195613 0.0 30-31 0.0 0.0 0.0 1.482204256110027 0.0 32-33 0.0 0.0 0.0 2.132701421800948 0.0 34-35 0.0 0.0 0.0 3.0085493913205092 0.0 36-37 0.0 0.0 0.0 3.8867205650032526 0.0 38-39 0.0 0.0 0.0 5.00882817582009 0.0 40-41 0.0 0.0 0.0 7.034662206114673 0.0 42-43 0.0 0.0 0.0 7.956974258897872 0.0 44-45 0.0 0.0 0.0 8.433231112350153 0.0 46-47 0.0 0.0 0.0 8.886255924170616 0.0 48-49 0.0 0.0 0.0 9.21382771117926 0.0 50-51 0.0 0.0 0.0 9.522813864882446 0.0 52-53 0.0 0.0 0.0 9.783012731158813 0.0 54-55 0.0 0.0 0.0 10.122200538983366 0.0 56-57 0.0 0.0 0.0 10.661183904841558 0.0 58-59 0.0 0.0 0.0 11.005018120992474 0.0 60-61 0.0 0.0 0.0 11.267540191432023 0.0 62-63 0.0 0.0 0.0 11.4626893411393 0.0 64-65 0.0 0.0 0.0 11.67642412415203 0.0 66-67 0.0 0.0 0.0 11.983087073692037 0.0 68-69 0.0 0.0 0.0 12.345506923148406 0.0 70-71 0.0 0.0 0.0 12.631261035219776 0.0 72-73 0.0 0.0 0.0 12.923984759780689 0.0 74-75 0.0 0.0 0.0 13.202769259362512 0.0 76-77 0.0 0.0 0.0 13.43741287984388 0.0 78-79 0.0 0.0 0.0 13.804479137626615 0.0 80-81 0.0 0.0 0.0 14.17154539540935 0.0 82-83 0.0 0.0 0.0 14.403865811727535 0.0 84-85 0.0 0.0 0.0 14.72214478208345 0.0 86-87 0.0 0.0 0.0 14.99860607750209 0.0 88-89 0.0 0.0 0.0 15.419106031038009 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 30 1.3221088E-6 79.16667 4 TATACAC 630 0.0 48.25397 3 CTTATAC 635 0.0 47.874012 1 TTATACA 640 0.0 47.499996 2 ATACACA 740 0.0 41.08108 4 TACACAT 745 0.0 40.16778 5 ACACATC 870 0.0 34.94253 6 CACATCT 890 0.0 34.157303 7 CATCTCC 895 0.0 33.96648 9 ACATCTC 900 0.0 33.77778 8 ACATGGG 100 1.2224209E-6 33.25 3 GTACATG 105 1.7895945E-6 31.666668 1 TACATGG 115 3.638008E-6 28.913044 2 GAGTACT 155 0.0 24.516129 1 AGTACTT 160 1.7224211E-6 23.749998 2 ACTTTTT 180 3.6379788E-12 21.11111 5 GTACTTT 190 9.094947E-12 20.0 3 TACTTTT 195 9.577383E-6 19.48718 4 CTTGAAA 845 0.0 18.550297 56-57 TGCTTGA 870 0.0 18.290232 54-55 >>END_MODULE