FastQCFastQC Report
Fri 10 Feb 2017
SRR2031841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37397
Sequences flagged as poor quality0
Sequence length101
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.5749124261304384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.5107361553065755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.29948926384469343No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.14974463192234672No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.11230847394176004No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.11230847394176004No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT390.10428644008877716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3050.043.6065561
GTATCAA4100.032.4390262
GGTCCCA300.003927552631.66666866-67
AGGGTCT300.003927552631.66666818-19
AACGCAG4050.030.4938287
TCAACGC4450.029.8876425
CAACGCA4350.029.4827586
TATCAAC4600.028.9130443
ATCAACG4650.028.602154
ACGCAGA4650.025.5376348
CGCAGAG4400.024.8295489
CATGGGG1000.001623981523.754
CAGAGTA4200.022.61904710-11
GAGTACT2450.021.3265321
GCAGAGT4500.021.111119
AGTACTT2503.8198777E-1120.9000022
GTACTTT2600.020.0961553
TACTTTT2751.72804E-1019.04
GAGTACA2400.018.80208412-13
TACATGG2402.931327E-617.81252
Produced by FastQC (version 0.11.5)