FastQCFastQC Report
Fri 10 Feb 2017
SRR2031841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37397
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT25166.727812391368292No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG13063.4922587373318716TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT9732.601812979650774RNA PCR Primer, Index 3 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT9272.478808460571704TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT4451.189935021525791TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA4321.1551728748295318RNA PCR Primer, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.7915073401609753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.7834853063079926No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1970.5267802230125411TruSeq Adapter, Index 3 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.4117977377864535No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1500.40110169264914297Illumina PCR Primer Index 12 (95% over 24bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.21659491403053724No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT580.15509265449100196No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC540.14439660935369147No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC440.11765649651041528No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT440.11765649651041528TruSeq Adapter, Index 3 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.11230847394176004No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.11230847394176004No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT400.1069604513731048No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG380.10161242880444955No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA250.00495729457.07561
GTGTAGC250.00495729457.07561
GGTATCA2200.051.886911
CGCAGAA557.9783604E-751.8174489
CCCTGGT459.5064304E-442.2216269
GTATCAA3550.037.514481
CTTATAC6850.036.800571
TTATACA7000.036.6423382
TATACAC7200.035.6244963
CGCAGAG3700.032.0941438
AACGCAG4000.032.0620466
TACACAT8200.031.8593085
CAACGCA4050.031.666225
TCAACGC4050.031.666224
ATACACA8400.031.1007524
ATCAACG4150.030.9031773
TATCAAC4200.030.5352842
ACGCAGA4300.029.8251617
GCAGAGT3900.029.2303569
ACACATC9150.028.551516
Produced by FastQC (version 0.11.5)