##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031841_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37397 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.775463272455006 34.0 31.0 34.0 31.0 34.0 2 32.901997486429394 34.0 33.0 34.0 31.0 34.0 3 32.9053400005348 34.0 33.0 34.0 31.0 34.0 4 36.33326202636575 37.0 37.0 37.0 35.0 37.0 5 36.328154664812686 37.0 37.0 37.0 35.0 37.0 6 36.41254646094607 37.0 37.0 37.0 35.0 37.0 7 36.36540364200337 37.0 37.0 37.0 35.0 37.0 8 36.403989624836214 37.0 37.0 37.0 35.0 37.0 9 38.21718319651309 39.0 39.0 39.0 37.0 39.0 10-11 38.20146001016124 39.0 39.0 39.0 37.0 39.0 12-13 38.205176885846456 39.0 39.0 39.0 37.0 39.0 14-15 39.69490868251464 41.0 40.0 41.0 37.0 41.0 16-17 39.60804342594326 41.0 40.0 41.0 37.0 41.0 18-19 39.68953391983314 41.0 40.0 41.0 37.0 41.0 20-21 39.64581383533439 41.0 40.0 41.0 37.0 41.0 22-23 39.5572773217103 41.0 40.0 41.0 36.5 41.0 24-25 39.37424124929807 41.0 39.0 41.0 35.5 41.0 26-27 39.12260341738642 41.0 39.0 41.0 35.0 41.0 28-29 38.881314009145115 41.0 38.0 41.0 35.0 41.0 30-31 38.47688317244699 40.0 38.0 41.0 35.0 41.0 32-33 37.829571890793375 40.0 36.5 41.0 33.5 41.0 34-35 37.77607829505041 40.0 37.0 41.0 33.0 41.0 36-37 37.59262775088911 40.0 37.0 41.0 33.0 41.0 38-39 37.042690590154294 40.0 35.0 41.0 32.0 41.0 40-41 36.88067224643688 40.0 35.5 41.0 31.5 41.0 42-43 36.6769660667968 40.0 35.0 41.0 30.5 41.0 44-45 36.19294328422066 39.0 35.0 41.0 29.0 41.0 46-47 35.70464208358959 39.0 35.0 41.0 25.0 41.0 48-49 35.485292937936194 39.0 35.0 41.0 23.0 41.0 50-51 35.04266385004145 39.0 35.0 41.0 20.0 41.0 52-53 35.00187180789903 39.0 35.0 41.0 20.0 41.0 54-55 34.86301040190389 39.0 35.0 41.0 20.0 41.0 56-57 34.15183036072412 37.5 34.5 40.0 19.0 41.0 58-59 33.435516217878444 37.0 33.5 40.0 16.0 41.0 60-61 32.44887290424366 35.5 32.5 39.0 10.5 41.0 62-63 31.457108858999383 35.0 30.5 39.0 8.0 41.0 64-65 30.668329010348423 35.0 28.5 38.5 7.5 41.0 66-67 29.722210337727624 35.0 24.5 37.0 7.0 40.5 68-69 28.700123004519078 35.0 21.0 36.5 7.0 39.5 70-71 27.782308741342888 35.0 17.0 36.0 7.0 39.0 72-73 27.13035805011097 35.0 12.5 35.0 7.0 38.5 74-75 26.906650266064123 35.0 11.0 35.0 7.0 37.0 76-77 26.050311522314622 32.5 12.5 35.0 7.0 36.0 78-79 26.698224456507205 34.5 15.0 35.0 7.0 36.0 80-81 26.698090755942992 35.0 13.5 35.0 7.0 36.0 82-83 26.520148675027407 35.0 11.0 35.0 7.0 35.5 84-85 26.34687274380298 34.0 10.0 35.0 7.0 35.0 86-87 26.234818300933227 34.0 10.0 35.0 7.0 35.0 88-89 26.138273123512583 34.0 10.0 35.0 7.0 35.0 90-91 26.020014974463194 34.0 10.0 35.0 7.0 35.0 92-93 25.93893895232238 34.0 10.0 35.0 7.0 35.0 94-95 25.90229162767067 34.0 10.0 35.0 7.0 35.0 96-97 25.826122416236593 34.0 10.0 35.0 7.0 35.0 98-99 25.780891515362192 34.0 10.0 35.0 7.0 35.0 100-101 24.6566970612616 31.5 10.0 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48264617402876553 1101 2 0.4602088795308319 1101 3 0.39858512142891556 1101 4 0.030838796536137636 1101 5 0.23862130137933946 1101 6 0.20346933281101087 1101 7 0.12215072067483135 1101 8 0.022251730092051503 1101 9 -0.3446647135747085 1101 10-11 0.02608261867369066 1101 12-13 0.17009780900918514 1101 14-15 0.31285736418865184 1101 16-17 0.3443722271429479 1101 18-19 0.06277868395162045 1101 20-21 0.5077823601228886 1101 22-23 0.4743623425993633 1101 24-25 0.548525577926064 1101 26-27 0.6163576661201873 1101 28-29 0.9041501264314746 1101 30-31 0.3720674116856415 1101 32-33 0.735226882467984 1101 34-35 0.6039993252457947 1101 36-37 1.0247251743015937 1101 38-39 -0.352855693067319 1101 40-41 1.0757716330459814 1101 42-43 0.2540219664781276 1101 44-45 0.9511173888300917 1101 46-47 1.641701257230558 1101 48-49 2.1972005959533263 1101 50-51 1.3942160984634242 1101 52-53 1.707894162614373 1101 54-55 1.6313662144993017 1101 56-57 1.2281464373825486 1101 58-59 1.9491726906618538 1101 60-61 0.8328137425412834 1101 62-63 1.752620127607205 1101 64-65 1.696052058165666 1101 66-67 1.4097014604471951 1101 68-69 -1.6899011814599199 1101 70-71 -1.7247449633665006 1101 72-73 -3.4013477327980333 1101 74-75 -2.901981351204725 1101 76-77 -1.0922057796455569 1101 78-79 -3.985304021394242 1101 80-81 -2.0931869075626572 1101 82-83 -3.0959474964332223 1101 84-85 -2.4344498206604612 1101 86-87 -3.303741521002319 1101 88-89 -2.1440967245431253 1101 90-91 -1.7749025100331046 1101 92-93 -2.993664427392673 1101 94-95 -3.4500359730835726 1101 96-97 -3.809346222128738 1101 98-99 -3.0992821394501426 1101 100-101 -2.8095206320083754 1102 1 -0.34799898726155476 1102 2 -0.4096067886719297 1102 3 0.012640420968089927 1102 4 0.28659916519973194 1102 5 0.14760747649454942 1102 6 0.2057734802764486 1102 7 -0.22001517794267755 1102 8 0.041836983548272144 1102 9 -0.2052637919157263 1102 10-11 0.048548056461719113 1102 12-13 -0.2550173983640889 1102 14-15 -0.0488937878850777 1102 16-17 0.3409160059447842 1102 18-19 -0.17800472618662866 1102 20-21 0.2986809776344117 1102 22-23 0.651781697438075 1102 24-25 0.6360831816126975 1102 26-27 -0.21255938457105827 1102 28-29 0.08617777620106182 1102 30-31 0.25513192781467353 1102 32-33 0.42531904836658896 1102 34-35 0.893745869024599 1102 36-37 -0.6238923372191394 1102 38-39 0.3107387769787735 1102 40-41 0.6005412182994405 1102 42-43 -0.17109324089513933 1102 44-45 0.42807591417571444 1102 46-47 -0.8300729363178263 1102 48-49 -0.0205413395305456 1102 50-51 -0.15762721950893166 1102 52-53 -0.7137648235607443 1102 54-55 0.5582095324716576 1102 56-57 0.7442754696406126 1102 58-59 1.705509096191804 1102 60-61 0.7838506089007353 1102 62-63 0.8897169018007567 1102 64-65 0.6315359291334097 1102 66-67 -0.7596533782624846 1102 68-69 -0.8454311353769661 1102 70-71 1.04484029008972 1102 72-73 0.8457720828701731 1102 74-75 0.36587579165241735 1102 76-77 -0.08471730038288783 1102 78-79 -0.5561565559564521 1102 80-81 0.6418361339119976 1102 82-83 -0.045832289059948295 1102 84-85 -0.38779083448534735 1102 86-87 -0.0508613366705255 1102 88-89 -0.8318847429763032 1102 90-91 -1.3042803902174391 1102 92-93 -1.4844478375309222 1102 94-95 -1.1216949592586865 1102 96-97 -1.7425259456310407 1102 98-99 -1.5750885910630466 1102 100-101 -0.9869399868470872 1103 1 0.6993128406954341 1103 2 0.4721136414355982 1103 3 0.8104898833336804 1103 4 0.5403626060599507 1103 5 0.4981451109031525 1103 6 0.493945523287195 1103 7 0.2197697682938795 1103 8 0.35796601580633336 1103 9 0.5291448102348113 1103 10-11 0.5915588091498805 1103 12-13 0.6439073328187064 1103 14-15 0.8580954594267496 1103 16-17 0.6586579414286575 1103 18-19 0.47468344585638533 1103 20-21 0.5804014077419382 1103 22-23 0.8576956759326961 1103 24-25 0.38662081602129916 1103 26-27 1.2175481423106547 1103 28-29 0.8851025073838557 1103 30-31 0.9804007450189758 1103 32-33 1.0495125967536936 1103 34-35 0.6313802776267536 1103 36-37 0.9223442219206461 1103 38-39 0.9209538307422065 1103 40-41 1.0817240139983682 1103 42-43 1.161164823620986 1103 44-45 1.3296888174015251 1103 46-47 2.2500345905638923 1103 48-49 1.8805339292866634 1103 50-51 1.691835146082461 1103 52-53 1.4840846388048448 1103 54-55 1.0420805002135936 1103 56-57 1.1781464373825514 1103 58-59 2.0813155478047065 1103 60-61 1.8435280282555695 1103 62-63 1.9859534609405394 1103 64-65 0.9293853914990002 1103 66-67 1.8597014604471944 1103 68-69 3.0362892947305617 1103 70-71 1.694302655681117 1103 72-73 2.368890362440066 1103 74-75 1.832542458319086 1103 76-77 1.1685085060687292 1103 78-79 0.032553121462900236 1103 80-81 0.6020511876754391 1103 82-83 0.8719096464239229 1103 84-85 0.41435970314906356 1103 86-87 1.1760203837595817 1103 88-89 1.63209375164735 1103 90-91 1.621526061395464 1103 92-93 1.3682403345120875 1103 94-95 1.026154503106902 1103 96-97 1.2811299683474537 1103 98-99 1.3507178605498567 1103 100-101 0.47381270132495956 1104 1 0.013896174028765529 1104 2 -0.017469691897737505 1104 3 -0.20409344999965384 1104 4 0.01744593939328354 1104 5 -0.46227155576351464 1104 6 0.06061218995386497 1104 7 0.27393643496054665 1104 8 0.2454660158063362 1104 9 0.13747814356814558 1104 10-11 -0.6792745241834481 1104 12-13 -0.12900933384796076 1104 14-15 -0.08669620723991756 1104 16-17 -0.003842058571343898 1104 18-19 0.0069751125230510524 1104 20-21 -0.18418192559139612 1104 22-23 -0.07251265740063673 1104 24-25 0.35432914935463344 1104 26-27 0.32171480897732607 1104 28-29 -0.35031415928281007 1104 30-31 0.2626924116856415 1104 32-33 0.32451259675369215 1104 34-35 0.9834636109600865 1104 36-37 1.114010888587309 1104 38-39 1.8636621640755422 1104 40-41 1.2588073473316967 1104 42-43 1.4861648236209888 1104 44-45 1.5984388174015223 1104 46-47 0.719826257230558 1104 48-49 1.1659505959533263 1104 50-51 2.1085018127491324 1104 52-53 1.819501305471512 1104 54-55 2.3858305002135936 1104 56-57 1.6656464373825486 1104 58-59 0.6031905478047079 1104 60-61 0.00915302825557518 1104 62-63 -0.7786298723927949 1104 64-65 -0.632072941834334 1104 66-67 -1.3715485395528049 1104 68-69 -1.515794038602774 1104 70-71 -2.173405677652216 1104 72-73 -2.1915263042266027 1104 74-75 -2.0716242083475827 1104 76-77 -2.165866493931272 1104 78-79 -1.5924468785370998 1104 80-81 -1.1333654789912266 1104 82-83 -2.0780903535760764 1104 84-85 -1.1866819635176036 1104 86-87 -1.3729379495737497 1104 88-89 -0.8606145816859829 1104 90-91 -1.4378489386045352 1104 92-93 0.21615700117875747 1104 94-95 -0.24467883022643022 1104 96-97 -0.8897033649858805 1104 98-99 -0.6774071394501426 1104 100-101 -1.1376456320083754 1105 1 0.4270906184732084 1105 2 0.3887803081022625 1105 3 0.2993787722225676 1105 4 0.39591816161550497 1105 5 0.3259228886809282 1105 6 0.31061218995386497 1105 7 0.33643643496054665 1105 8 0.19685490469522193 1105 9 0.5958114769014813 1105 10-11 0.09155880914988046 1105 12-13 0.48557399948536784 1105 14-15 0.6580954594267467 1105 16-17 0.8364357192064347 1105 18-19 0.9496834458563796 1105 20-21 0.5137347410752753 1105 22-23 0.7521401203771418 1105 24-25 0.9116208160213048 1105 26-27 1.0925481423106547 1105 28-29 1.1878802851616328 1105 30-31 1.45540074501897 1105 32-33 1.7411792634203564 1105 34-35 0.8897136109600865 1105 36-37 1.4473442219206376 1105 38-39 1.3653982751866565 1105 40-41 1.456724013998361 1105 42-43 1.9028314902876602 1105 44-45 2.1713554840681937 1105 46-47 2.0028123683416723 1105 48-49 2.4888672626199977 1105 50-51 2.6085018127491324 1105 52-53 2.156306861027069 1105 54-55 2.1254138335469293 1105 56-57 0.9364797707158772 1105 58-59 1.2229822144713793 1105 60-61 1.5490835838111323 1105 62-63 0.7387312387183194 1105 64-65 0.6543853914989981 1105 66-67 0.34025701600275227 1105 68-69 0.5501781836194475 1105 70-71 -0.17514178876332664 1105 72-73 -0.7783318597821598 1105 74-75 -1.3424575416809184 1105 76-77 -1.667602605042383 1105 78-79 -1.148002434092657 1105 80-81 -0.9840599234356695 1105 82-83 -1.3697570202427407 1105 84-85 -0.7856402968509357 1105 86-87 -0.7323129495737497 1105 88-89 -0.30679513724154006 1105 90-91 0.032637172506575496 1105 92-93 -0.16231522104346396 1105 94-95 -0.7238454968930945 1105 96-97 -0.10498114276365911 1105 98-99 -0.8492821394501426 1105 100-101 -2.2261872986750397 1106 1 0.6871916285742188 1106 2 0.9872651565871138 1106 3 0.7438232166670105 1106 4 0.370665636362979 1106 5 0.5102663230243607 1106 6 0.4394000687417403 1106 7 0.465224313748422 1106 8 0.43675389459421154 1106 9 0.4291448102348099 1106 10-11 0.5006497182407941 1106 12-13 0.5651194540308211 1106 14-15 0.6353681866994734 1106 16-17 0.4601730929438119 1106 18-19 0.49892587009880884 1106 20-21 0.5288862562267838 1106 22-23 0.7622411304781522 1106 24-25 0.52904505844554 1106 26-27 0.2933057180682326 1106 28-29 0.19419341647476784 1106 30-31 0.4213098359280636 1106 32-33 0.487391384632474 1106 34-35 0.15865300489947742 1106 36-37 0.2617381613145824 1106 38-39 -0.344197684409302 1106 40-41 -1.1834275011531474 1106 42-43 -0.9570169945608242 1106 44-45 -1.0445536068408998 1106 46-47 -2.127238136708833 1106 48-49 -1.9467387979860646 1106 50-51 -0.7324072781599611 1106 52-53 -1.0280365733163634 1106 54-55 -2.4579194997864064 1106 56-57 -2.9536717444356313 1106 58-59 -1.0989874824983232 1106 60-61 -0.5064719717444248 1106 62-63 -0.45192532693824816 1106 64-65 -1.1524327903191853 1106 66-67 -0.9766621759164416 1106 68-69 -0.08492282648156291 1106 70-71 -0.4541821928037315 1106 72-73 -0.21141266786296598 1106 74-75 0.9264818522584797 1106 76-77 0.15184183940205997 1106 78-79 -0.2629014239916465 1106 80-81 -0.7555245699003166 1106 82-83 -0.881120656606381 1106 84-85 -0.46745847866911916 1106 86-87 -0.38761597987678087 1106 88-89 -0.008815339261740718 1106 90-91 0.573041212910617 1106 92-93 0.843997910269664 1106 94-95 0.2610029879553899 1106 96-97 0.2144633016807873 1106 98-99 0.3022330120650061 1106 100-101 0.6147217922340467 1107 1 0.4431724898182381 1107 2 0.34930662389173506 1107 3 0.5508407605266612 1107 4 0.09474857097222866 1107 5 -0.18431102944772704 1107 6 -0.2814930732040324 1107 7 0.06012064548686169 1107 8 0.16322917370107604 1107 9 -0.05331133011606681 1107 10-11 0.179278107395497 1107 12-13 0.10618803457309411 1107 14-15 0.29406037170745236 1107 16-17 0.46655267827076585 1107 18-19 0.13608695462831122 1107 20-21 0.3996996533559738 1107 22-23 -0.4861639731901093 1107 24-25 0.10679625461779096 1107 26-27 0.02895165108258624 1107 28-29 -0.24998521191438527 1107 30-31 -0.1213536409459337 1107 32-33 0.31793364938526736 1107 34-35 -0.39318112588202325 1107 36-37 -1.4978312166758485 1107 38-39 -1.9036075727665605 1107 40-41 -1.3086268631946183 1107 42-43 -0.8493614921684909 1107 44-45 -0.35715328786163525 1107 46-47 -1.404351374348387 1107 48-49 -0.7238520356256188 1107 50-51 -0.9178139767245526 1107 52-53 -0.24135395768637835 1107 54-55 -0.8131826576811463 1107 56-57 -1.1402746152490266 1107 58-59 -0.6739476100900319 1107 60-61 -0.5064719717444248 1107 62-63 0.0026201276072050916 1107 64-65 -1.0671058365711765 1107 66-67 -0.51135117113175 1107 68-69 0.40032438244985613 1107 70-71 0.4951798486635717 1107 72-73 1.2443289589312911 1107 74-75 0.7342968442839961 1107 76-77 -0.18149149393127217 1107 78-79 -0.4279731943265723 1107 80-81 -0.9277733737280691 1107 82-83 -1.262300879891864 1107 84-85 -0.9259911740439186 1107 86-87 -0.38362873904743466 1107 88-89 -0.3828185290544006 1107 90-91 0.1583681666586223 1107 92-93 0.26385436959981234 1107 94-95 0.28492643293146713 1107 96-97 0.6662176876456982 1107 98-99 0.33492838686564497 1107 100-101 0.4141635785179396 1108 1 0.3642251213971832 1108 2 0.03351715020752266 1108 3 0.182419707895086 1108 4 -0.06314616586987398 1108 5 0.1051626547628004 1108 6 -0.018335178467189905 1108 7 0.11275222443423161 1108 8 -0.07361293156208149 1108 9 0.28879393304183054 1108 10-11 -0.018090313657133095 1108 12-13 0.27724066615203924 1108 14-15 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-2.8743096236351384 2316 80-81 -2.9391252829127943 2316 82-83 -3.087894275144702 2316 84-85 -3.1679932380274067 2316 86-87 -3.465156086828653 2316 88-89 -3.9767297777644153 2316 90-91 -3.6372974680163033 2316 92-93 -2.790583194899675 2316 94-95 -4.145414124344075 2316 96-97 -4.060046502240784 2316 98-99 -3.947321355136417 2316 100-101 -4.76050102416524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 7.0 15 32.0 16 113.0 17 284.0 18 424.0 19 481.0 20 569.0 21 654.0 22 750.0 23 694.0 24 734.0 25 1055.0 26 1780.0 27 1641.0 28 1648.0 29 1629.0 30 677.0 31 439.0 32 452.0 33 677.0 34 967.0 35 1871.0 36 4328.0 37 8052.0 38 6441.0 39 995.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.98339937881546 17.644853807432796 13.593766734497162 28.77798007925458 2 15.102815733882396 17.824959221327912 39.34272802631227 27.729497018477417 3 16.08685188651496 25.32823488515121 35.810359119715486 22.77455410861834 4 10.230767173837474 21.89747840735888 27.590448431692383 40.28130598711127 5 12.316495975613018 33.909137096558545 35.36112522394845 18.41324170387999 6 29.202877236141937 35.107094151937325 15.330106693050244 20.359921918870498 7 23.18902585768912 32.374254619354495 16.340882958526084 28.095836564430304 8 27.069016231248494 31.26453993635853 21.442896489023184 20.223547343369788 9 22.183597614781934 11.573120838569938 15.068053587186139 51.17522795946199 10-11 23.246517100302164 23.3708586250234 25.764098724496616 27.618525550177818 12-13 25.952616520041715 21.539160895258977 24.21049816830227 28.297724416397035 14-15 23.727839131481137 23.124849586865256 20.889376153167365 32.257935128486245 16-17 24.425756076690643 29.170789100730005 21.04446880765837 25.35898601492098 18-19 26.123753242238685 21.59264112094553 24.7412894082413 27.54231622857449 20-21 23.151589699708534 22.630157499264648 26.499451827686716 27.71880097334011 22-23 22.995160039575367 28.561114527903307 24.955210310987514 23.488515121533812 24-25 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18.49638335138315 30.521572874466862 27.81142620298691 50-51 21.74639676979437 21.833302136535014 30.6227772281199 25.797523865550716 52-53 19.920313398312675 24.483574666078376 33.116735523377855 22.47937641223109 54-55 19.88662192154451 25.544829799181752 28.45549108217237 26.11305719710137 56-57 22.86547049228548 28.761665374227878 23.779982351525522 24.592881781961122 58-59 18.603096505067253 31.543974115570766 28.84322271839987 21.00970666096211 60-61 22.10605128753643 35.45471561889991 21.056501858437844 21.382731235125814 62-63 18.847768537583228 38.640800064176275 20.639356098082732 21.872075300157768 64-65 19.04430836698131 37.81453057731904 20.53640666363612 22.604754392063533 66-67 19.01355723721154 36.611225499371606 20.710217397117418 23.664999866299436 68-69 20.213121548808044 35.19045900017381 20.73456072092308 23.86185873009506 70-71 20.656737171430866 33.67382410353772 21.081904965638955 24.587533759392464 72-73 21.224964569350483 31.545311121212933 21.587293098376875 25.642431211059712 74-75 21.508409765489212 30.68829050458593 22.190282642992752 25.613017086932103 76-77 21.915152487532254 30.7675852018237 21.650421830920006 25.66684047972404 78-79 22.615450437200845 29.757734577639916 21.81993208011338 25.806882905045857 80-81 22.829371339947055 29.316522715725863 21.758429820573845 26.095676123753243 82-83 22.801294221461614 28.642671872075297 22.398855523170308 26.157178383292777 84-85 23.324063427547664 27.861860577051633 22.409551568307617 26.404524427093083 86-87 23.496537155386797 27.753563120036368 22.08064818033532 26.66925154424152 88-89 24.103537716929164 27.4273337433484 22.184934620424098 26.28419391929834 90-91 23.717143086343825 26.713372730432923 22.725084899858278 26.84439928336498 92-93 23.43904591277375 26.216006631547984 23.43503489584726 26.909912559831 94-95 23.612856646255047 25.87105917586972 23.572746476990133 26.9433377008851 96-97 23.242506083375673 25.673182340829477 23.83881059978073 27.24550097601412 98-99 23.197047891542102 25.933898441051422 23.850843650560204 27.01821001684627 100-101 23.507233200524105 25.777468780918255 23.916356927026232 26.798941091531407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 27.0 1 19.0 2 13.5 3 16.5 4 14.5 5 11.5 6 14.0 7 28.0 8 53.5 9 62.5 10 51.5 11 43.0 12 40.5 13 42.0 14 49.0 15 54.5 16 53.0 17 58.0 18 72.0 19 77.5 20 79.5 21 89.5 22 106.5 23 118.0 24 141.0 25 163.0 26 160.0 27 186.5 28 220.5 29 244.5 30 298.0 31 353.0 32 391.5 33 426.5 34 476.0 35 583.5 36 720.0 37 845.5 38 951.0 39 999.0 40 1044.0 41 1114.5 42 1163.5 43 1125.5 44 1077.0 45 1074.0 46 1037.5 47 1017.5 48 1012.0 49 1103.5 50 1304.0 51 1321.5 52 1082.0 53 874.0 54 815.0 55 841.0 56 874.5 57 900.0 58 966.0 59 1104.5 60 1192.0 61 1147.0 62 1089.5 63 985.5 64 842.0 65 710.5 66 548.5 67 413.5 68 319.0 69 232.0 70 175.0 71 137.0 72 103.5 73 81.0 74 70.0 75 47.0 76 34.5 77 23.0 78 12.0 79 11.0 80 7.0 81 8.5 82 8.5 83 2.5 84 0.5 85 1.0 86 0.5 87 1.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13102655293205337 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0013370056421638098 44-45 0.0 46-47 0.0 48-49 0.0013370056421638098 50-51 0.0 52-53 0.0013370056421638098 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0013370056421638098 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0013370056421638098 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 37397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.43321656817392 #Duplication Level Percentage of deduplicated Percentage of total 1 91.00642398286938 61.36855897531888 2 6.221746371639305 8.39104741022007 3 1.502894757712745 3.0403508302805036 4 0.4798160044412721 1.294221461614568 5 0.24585613450709812 0.8289434981415622 6 0.14672059639939727 0.5936305051207317 7 0.05155047981600444 0.24333502687381342 8 0.04758505829169641 0.2567050832954515 9 0.04361963676738837 0.2647271171484344 >10 0.1982710762154017 2.6419231489156885 >50 0.011896264572924102 0.5160841778752306 >100 0.02775795067015624 5.259780196272428 >500 0.007930843048616068 5.080621440222478 >1k 0.007930843048616068 10.220071128700162 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2516 6.727812391368292 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 1306 3.4922587373318716 TruSeq Adapter, Index 3 (96% over 28bp) ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 973 2.601812979650774 RNA PCR Primer, Index 3 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 927 2.478808460571704 TruSeq Adapter, Index 3 (95% over 21bp) CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT 445 1.189935021525791 TruSeq Adapter, Index 3 (96% over 26bp) CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA 432 1.1551728748295318 RNA PCR Primer, Index 3 (96% over 28bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 296 0.7915073401609753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.7834853063079926 No Hit CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG 197 0.5267802230125411 TruSeq Adapter, Index 3 (95% over 24bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.4117977377864535 No Hit ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC 150 0.40110169264914297 Illumina PCR Primer Index 12 (95% over 24bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.21659491403053724 No Hit TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT 58 0.15509265449100196 No Hit TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC 54 0.14439660935369147 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC 44 0.11765649651041528 No Hit ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT 44 0.11765649651041528 TruSeq Adapter, Index 3 (96% over 27bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42 0.11230847394176004 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42 0.11230847394176004 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT 40 0.1069604513731048 No Hit GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG 38 0.10161242880444955 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.00267401128432762 0.0 10-11 0.0 0.0 0.0 0.00267401128432762 0.0 12-13 0.0 0.0 0.0 0.00267401128432762 0.0 14-15 0.0 0.0 0.0 0.00267401128432762 0.0 16-17 0.0 0.0 0.0 0.00267401128432762 0.0 18-19 0.0 0.0 0.0 0.00267401128432762 0.0 20-21 0.0 0.0 0.0 0.00802203385298286 0.0 22-23 0.0 0.0 0.0 0.03208813541193144 0.0 24-25 0.0 0.0 0.0 0.09760141187795812 0.0 26-27 0.0 0.0 0.0 0.21258389710404577 0.0 28-29 0.0 0.0 0.0 0.41179773778645345 0.0 30-31 0.0 0.0 0.0 0.7286680749792764 0.0 32-33 0.0 0.0 0.0 1.0575714629515738 0.0 34-35 0.0 0.0 0.0 1.5830146803219511 0.0 36-37 0.0 0.0 0.0 2.149905072599406 0.0 38-39 0.0 0.0 0.0 2.780971735700725 0.0 40-41 0.0 0.0 0.0 3.8331951760836436 0.0 42-43 0.0 0.0 0.0 4.373345455517823 0.0 44-45 0.0 0.0 0.0 4.728988956333396 0.0 46-47 0.0 0.0 0.0 5.069925395085168 0.0 48-49 0.0 0.0 0.0 5.361392625076878 0.0 50-51 0.0 0.0 0.0 5.6394897986469505 0.00267401128432762 52-53 0.0 0.0 0.0 5.852073695750995 0.00267401128432762 54-55 0.0 0.0 0.0 6.130170869321068 0.00267401128432762 56-57 0.0 0.0 0.0 6.491162392705297 0.00267401128432762 58-59 0.0 0.0 0.0 6.74118244778993 0.00267401128432762 60-61 0.0 0.0 0.0 6.933711260261518 0.00267401128432762 62-63 0.0 0.0 0.0 7.120892050164452 0.00267401128432762 64-65 0.0 0.0 0.0 7.301387811856566 0.00267401128432762 66-67 0.0 0.0 0.0 7.5447228387303795 0.00267401128432762 68-69 0.0 0.0 0.0 7.810786961520978 0.00267401128432762 70-71 0.0 0.0 0.0 8.044762948899645 0.00267401128432762 72-73 0.0 0.0 0.0 8.273390913709655 0.00267401128432762 74-75 0.0 0.0 0.0 8.452549669759607 0.00267401128432762 76-77 0.0 0.0 0.0 8.655774527368505 0.00267401128432762 78-79 0.0 0.0 0.0 8.854988368050915 0.00267401128432762 80-81 0.0 0.0 0.0 9.035484129743029 0.00267401128432762 82-83 0.0 0.0 0.0 9.221327914003798 0.00267401128432762 84-85 0.0 0.0 0.0 9.416530737759713 0.00267401128432762 86-87 0.0 0.0 0.0 9.663876781560019 0.00267401128432762 88-89 0.0 0.0 0.0 9.994117175174479 0.00267401128432762 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 25 0.004957294 57.0756 1 GTGTAGC 25 0.004957294 57.0756 1 GGTATCA 220 0.0 51.88691 1 CGCAGAA 55 7.9783604E-7 51.817448 9 CCCTGGT 45 9.5064304E-4 42.221626 9 GTATCAA 355 0.0 37.51448 1 CTTATAC 685 0.0 36.80057 1 TTATACA 700 0.0 36.642338 2 TATACAC 720 0.0 35.624496 3 CGCAGAG 370 0.0 32.094143 8 AACGCAG 400 0.0 32.062046 6 TACACAT 820 0.0 31.859308 5 CAACGCA 405 0.0 31.66622 5 TCAACGC 405 0.0 31.66622 4 ATACACA 840 0.0 31.100752 4 ATCAACG 415 0.0 30.903177 3 TATCAAC 420 0.0 30.535284 2 ACGCAGA 430 0.0 29.825161 7 GCAGAGT 390 0.0 29.230356 9 ACACATC 915 0.0 28.55151 6 >>END_MODULE