FastQCFastQC Report
Fri 10 Feb 2017
SRR2031840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7858
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1241.578009671672181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1081.374395520488674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.7635530669381522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG150.19088826673453804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG140.17816238228556885No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.16543649783659964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.13998472893866123No Hit
GTACATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA110.13998472893866123No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.13998472893866123No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA110.13998472893866123No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA100.12725884448969205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.12725884448969205No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.12725884448969205No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11453296004072283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC90.11453296004072283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.11453296004072283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA90.11453296004072283No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10180707559175363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA80.10180707559175363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1250.053.1999971
AACGCAG1900.035.06
GAGTACT1100.034.54545612-13
GTATCAA1950.034.1025621
TCAACGC1950.034.1025624
CAACGCA1950.034.1025625
CGCAGAG2100.033.928578
ACGCAGA2050.032.4390227
TATCAAC2050.032.4390222
GTACTTT1200.031.66666814-15
GCAGAGT2100.031.6666669
ATCAACG2100.031.6666663
CAGAGTA2150.030.93023110-11
ACTTTTT1400.028.83928716-17
ACATGGG853.092282E-927.94117516-17
GTACATG905.691618E-926.38888714-15
GAGTACA1005.9117156E-1026.12512-13
AGTACTT1104.8035872E-821.5909112-13
TACTTTT1201.2052806E-719.79166814-15
AGAGTAC2051.8189894E-1218.53658510-11
Produced by FastQC (version 0.11.5)