FastQCFastQC Report
Fri 10 Feb 2017
SRR2031840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7858
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1822.316110969712395No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT1752.2270297785696105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1612.0488673962840416No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1301.6543649783659964TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT981.247136675998982TruSeq Adapter, Index 3 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT851.0817001781623823No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT760.9671672181216595RNA PCR Primer, Index 3 (95% over 23bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA550.6999236446933061RNA PCR Primer, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT500.6362942224484602TruSeq Adapter, Index 3 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.27996945787732247No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG210.26724357342835325TruSeq Adapter, Index 3 (95% over 24bp)
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC170.21634003563247647Illumina PCR Primer Index 12 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.21634003563247647No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT120.15271061338763045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC110.13998472893866123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.13998472893866123No Hit
GTACATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA110.13998472893866123No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.13998472893866123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC110.13998472893866123No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA100.12725884448969205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG100.12725884448969205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.12725884448969205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG100.12725884448969205No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11453296004072283No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11453296004072283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG80.10180707559175363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.10180707559175363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC80.10180707559175363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1101.1514203E-939.110141
AACGCAG1506.82121E-1031.6645436
GTATCAA1601.2714736E-929.8758011
TATCAAC1801.4188117E-1029.0258332
TCAACGC1651.897206E-928.785954
GCAGAGT1651.897206E-928.785959
CAACGCA1651.897206E-928.785955
GAGTACT1250.028.49808912-13
ACGCAGA1702.6102498E-927.9393027
CGCAGAG1702.6102498E-927.9393028
ATCAACG1702.6102498E-927.9393023
GTACTTT1300.027.40200814-15
ACTTTTT1400.025.44472316-17
CAGAGTA1600.025.23268310-11
CTTATAC950.001080141525.1585671
TATACAC950.00112183924.9983233
ATACACA950.00112183924.9983234
TTATACA1000.001512965723.7484072
TACACAT1000.001512965723.7484075
ACACATC1050.002010044622.617536
Produced by FastQC (version 0.11.5)