##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031840_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7858 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69088826673454 34.0 31.0 34.0 31.0 34.0 2 32.79969457877323 34.0 31.0 34.0 31.0 34.0 3 32.816238228556884 34.0 31.0 34.0 31.0 34.0 4 36.278187834054464 37.0 37.0 37.0 35.0 37.0 5 36.25693560702469 37.0 37.0 37.0 35.0 37.0 6 36.38203105115806 37.0 37.0 37.0 35.0 37.0 7 36.30656655637567 37.0 37.0 37.0 35.0 37.0 8 36.36777806057521 37.0 37.0 37.0 35.0 37.0 9 38.12013234919827 39.0 39.0 39.0 37.0 39.0 10-11 38.16486383303639 39.0 39.0 39.0 37.0 39.0 12-13 38.151119877831505 39.0 39.0 39.0 37.0 39.0 14-15 39.501972512089594 41.0 39.5 41.0 36.5 41.0 16-17 39.464685670654106 41.0 39.0 41.0 36.5 41.0 18-19 39.68306184779842 41.0 40.0 41.0 37.0 41.0 20-21 39.70628658691779 41.0 40.0 41.0 37.0 41.0 22-23 39.62694069737847 41.0 40.0 41.0 36.5 41.0 24-25 39.473530160346144 41.0 39.5 41.0 35.5 41.0 26-27 39.20832272842962 41.0 39.0 41.0 35.0 41.0 28-29 39.001145329600405 41.0 38.5 41.0 35.0 41.0 30-31 38.51151692542632 40.0 37.5 41.0 35.0 41.0 32-33 37.63247645711377 39.5 35.0 41.0 33.5 41.0 34-35 37.48364723848307 40.0 35.0 41.0 33.0 41.0 36-37 37.20208704504963 40.0 35.0 41.0 33.0 41.0 38-39 36.87185034359888 40.0 35.0 41.0 33.0 41.0 40-41 36.47811147874778 39.0 35.0 41.0 31.5 41.0 42-43 36.06286586917791 39.0 35.0 41.0 29.5 41.0 44-45 35.33882667345381 39.0 35.0 41.0 23.0 41.0 46-47 34.337872232120134 39.0 34.5 41.0 15.0 41.0 48-49 33.458449987274115 39.0 33.0 41.0 9.0 41.0 50-51 32.82151947060321 39.0 33.0 41.0 7.0 41.0 52-53 32.609188088572154 39.0 33.0 41.0 7.0 41.0 54-55 32.370323237465 38.5 32.0 41.0 7.0 41.0 56-57 31.87668617968949 37.5 30.5 41.0 7.0 41.0 58-59 31.325719012471367 37.0 27.5 41.0 7.0 41.0 60-61 30.64246627640621 36.0 23.5 40.0 7.0 41.0 62-63 30.03086026978875 35.0 21.0 40.0 7.0 41.0 64-65 29.447951132603716 35.0 19.5 39.0 7.0 41.0 66-67 28.91562738610333 35.0 18.0 39.0 7.0 41.0 68-69 28.193242555357596 35.0 14.0 38.5 7.0 41.0 70-71 27.56331127513362 35.0 10.0 37.0 7.0 40.0 72-73 27.043140748282006 35.0 10.0 37.0 7.0 39.0 74-75 26.687452277933318 35.0 10.0 36.0 7.0 39.0 76-77 25.601552557902775 32.5 10.0 35.0 7.0 37.0 78-79 26.167345380503946 34.0 10.0 35.0 7.0 37.0 80-81 26.150356324764573 35.0 10.0 35.0 7.0 37.0 82-83 25.860206159328072 34.0 10.0 35.0 7.0 36.5 84-85 25.627004326800716 34.0 10.0 35.0 7.0 36.0 86-87 25.519088826673453 34.0 10.0 35.0 7.0 36.0 88-89 25.29059557139221 34.0 10.0 35.0 7.0 35.5 90-91 25.129040468312546 34.0 10.0 35.0 7.0 35.0 92-93 25.046004072283026 34.0 10.0 35.0 7.0 35.0 94-95 24.90582845507763 34.0 10.0 35.0 7.0 35.0 96-97 24.76953423262917 34.0 8.0 35.0 7.0 35.0 98-99 24.717612624077375 34.0 9.0 35.0 7.0 35.0 100-101 23.74713667599898 31.5 7.5 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.8858213545713518 1101 2 -0.7519489191364102 1101 3 0.544291009916023 1101 4 -0.2251141219891366 1101 5 -0.27929050116551224 1101 6 0.2992767359954769 1101 7 0.8112027729215114 1101 8 0.6454717157842182 1101 9 0.8727443563380959 1101 10-11 0.5268885772792089 1101 12-13 0.6797833243145774 1101 14-15 0.9912651584526699 1101 16-17 1.4085056581150397 1101 18-19 1.310928018740519 1101 20-21 0.10256342326654533 1101 22-23 -0.5614799696831057 1101 24-25 -0.7837811783124238 1101 26-27 -0.6240920104201351 1101 28-29 -0.48715107230732 1101 30-31 -0.2003300085331432 1101 32-33 -1.1925925058737548 1101 34-35 -2.6345135622479248 1101 36-37 -5.472924977221844 1101 38-39 -7.591354146432266 1101 40-41 -5.875627063908318 1101 42-43 -7.559103252072006 1101 44-45 -6.578690031033787 1101 46-47 -5.963977125305242 1101 48-49 -4.13926678067304 1101 50-51 -2.868564667392789 1101 52-53 -2.1992441586191624 1101 54-55 -2.183621919168793 1101 56-57 -2.4236166611166716 1101 58-59 -1.8469951778545486 1101 60-61 -1.3594564868002443 1101 62-63 -1.4830401080401003 1101 64-65 -1.6955564979002489 1101 66-67 -1.4282067903161533 1101 68-69 -1.0047295730889552 1101 70-71 1.001673080969944 1101 72-73 -0.08996014113202477 1101 74-75 -0.27634016735579436 1101 76-77 0.013216630013491937 1101 78-79 1.7170708198051798 1101 80-81 -0.5916170201326381 1101 82-83 -0.14699076964701874 1101 84-85 -0.35484707711270147 1101 86-87 -0.5858822456478734 1101 88-89 0.16348856583229932 1101 90-91 2.0834313834313853 1101 92-93 2.1076850088568833 1101 94-95 4.262741410788287 1101 96-97 1.8006069162319065 1101 98-99 0.326989546911431 1101 100-101 0.8823652909590471 1102 1 0.32251197876198034 1102 2 -0.0019489191364101544 1102 3 -0.9140423234173127 1102 4 0.024885878010863394 1102 5 0.7207094988344878 1102 6 0.3826100693288126 1102 7 0.31120277292151144 1102 8 0.27047171578421825 1102 9 0.3727443563380959 1102 10-11 0.4435552439458803 1102 12-13 0.5339499909812488 1102 14-15 -0.5504015082139944 1102 16-17 -0.5706610085516317 1102 18-19 -0.0015719812594809923 1102 20-21 0.22756342326654533 1102 22-23 0.646853363650223 1102 24-25 0.19538548835424052 1102 26-27 1.063407989579865 1102 28-29 1.32534892769268 1102 30-31 0.6538366581335282 1102 32-33 -0.00509250587375476 1102 34-35 -0.7386802289145962 1102 36-37 -0.5770916438885081 1102 38-39 -0.6538541464322662 1102 40-41 -0.12562706390831835 1102 42-43 0.357563414594658 1102 44-45 1.2546433022995487 1102 46-47 0.8485228746947584 1102 48-49 -3.88926678067304 1102 50-51 -6.681064667392789 1102 52-53 -6.24091082528583 1102 54-55 -6.183621919168793 1102 56-57 -5.881949994450004 1102 58-59 -3.6803285111878843 1102 60-61 -5.088623153466909 1102 62-63 -5.6080401080401 1102 64-65 -4.903889831233585 1102 66-67 -3.8032067903161533 1102 68-69 -2.504729573088955 1102 70-71 -5.144160252363392 1102 72-73 -2.9649601411320248 1102 74-75 -3.1513401673557944 1102 76-77 -3.424283369986508 1102 78-79 -3.5745958468614845 1102 80-81 -3.133283686799306 1102 82-83 -5.292824102980351 1102 84-85 -4.334013743779366 1102 86-87 -3.7525489123145377 1102 88-89 -3.565678100834365 1102 90-91 -5.291568616568615 1102 92-93 -5.183981657809781 1102 94-95 -3.9455919225450486 1102 96-97 -5.178559750434758 1102 98-99 -3.6938437864219047 1102 100-101 -5.263468042374289 1103 1 1.0189405501905497 1103 2 0.6766225094350204 1103 3 0.7823862480112567 1103 4 0.4891715922965787 1103 5 0.43499521312020306 1103 6 0.6326100693288126 1103 7 0.5254884872072267 1103 8 0.6454717157842182 1103 9 0.8727443563380959 1103 10-11 0.6578409582315956 1103 12-13 0.48930713383838764 1103 14-15 1.146027063214575 1103 16-17 0.9918389914483683 1103 18-19 0.8823565901690884 1103 20-21 0.3168491375522535 1103 22-23 0.41471050650736174 1103 24-25 0.3114569169256711 1103 26-27 0.9830508467227261 1103 28-29 0.26284892769268 1103 30-31 1.7163366581335282 1103 32-33 0.05740749412624524 1103 34-35 0.19881977108540383 1103 36-37 1.2175512132543531 1103 38-39 1.6586458535677338 1103 40-41 2.5529443646631123 1103 42-43 2.964706271737512 1103 44-45 2.1832147308709793 1103 46-47 4.107451446123328 1103 48-49 5.182161790755529 1103 50-51 6.20286390403578 1103 52-53 6.5626606032856 1103 54-55 6.780663795116922 1103 56-57 6.7073357198357115 1103 58-59 7.033957203097831 1103 60-61 7.1881625608188 1103 62-63 7.588388463388469 1103 64-65 6.113967311623554 1103 66-67 7.571793209683847 1103 68-69 7.45955614119676 1103 70-71 7.204054033350893 1103 72-73 7.69575414458226 1103 74-75 8.259374118358497 1103 76-77 6.9417880585849225 1103 78-79 7.586118438852807 1103 80-81 7.170287741772125 1103 82-83 7.44824732559108 1103 84-85 8.085629113363495 1103 86-87 8.033165373399754 1103 88-89 8.889679042022774 1103 90-91 8.904859954859955 1103 92-93 8.36958977076165 1103 94-95 7.715122363169236 1103 96-97 8.919654535279527 1103 98-99 8.850799070720957 1103 100-101 8.263317671911427 1104 1 -0.26677373552373496 1104 2 0.9623367951493051 1104 3 0.9252433908684026 1104 4 0.4891715922965787 1104 5 0.43499521312020306 1104 6 0.6326100693288126 1104 7 0.8112027729215114 1104 8 0.6454717157842182 1104 9 0.72988721348095 1104 10-11 0.5149838153744497 1104 12-13 0.060735705266964146 1104 14-15 0.646027063214575 1104 16-17 0.9204104200197989 1104 18-19 1.0966423044548037 1104 20-21 0.17399199469511473 1104 22-23 -0.6567180649212077 1104 24-25 -0.04568594021719008 1104 26-27 0.19733656100843433 1104 28-29 0.8342774991212494 1104 30-31 0.0020509438478129027 1104 32-33 -0.15687822015947006 1104 34-35 0.2702483425139732 1104 36-37 1.7175512132543531 1104 38-39 2.3015029964248725 1104 40-41 2.481515793234543 1104 42-43 2.89327770030895 1104 44-45 3.826071873728118 1104 46-47 4.3931657318376125 1104 48-49 5.61073321932696 1104 50-51 6.345721046892926 1104 52-53 6.7769463175713085 1104 54-55 6.709235223688346 1104 56-57 7.2073357198357115 1104 58-59 7.4625286316692545 1104 60-61 8.11673398939023 1104 62-63 8.231245606245608 1104 64-65 7.899681597337846 1104 66-67 8.643221781112416 1104 68-69 8.888127569768184 1104 70-71 9.132625461922323 1104 72-73 8.195754144582267 1104 74-75 7.687945546929921 1104 76-77 7.9417880585849225 1104 78-79 8.01468986742423 1104 80-81 8.170287741772125 1104 82-83 8.591104468448219 1104 84-85 7.157057684792065 1104 86-87 7.890308230542601 1104 88-89 9.246821899165635 1104 90-91 6.904859954859955 1104 92-93 8.79816119933308 1104 94-95 6.857979506026382 1104 96-97 8.991083106708103 1104 98-99 7.493656213578095 1104 100-101 7.263317671911427 1105 1 -0.052488021238019655 1105 2 0.7480510808635898 1105 3 0.37762434324935157 1105 4 0.1082192113441991 1105 5 -0.27929050116551224 1105 6 -0.03405659733785171 1105 7 0.47786943958817574 1105 8 0.6454717157842182 1105 9 0.8727443563380959 1105 10-11 0.2768885772792089 1105 12-13 0.8464499909812488 1105 14-15 1.4079318251193342 1105 16-17 0.9085056581150326 1105 18-19 0.560928018740519 1105 20-21 1.0192300899332167 1105 22-23 1.1885200303168943 1105 24-25 1.2162188216875691 1105 26-27 1.625907989579865 1105 28-29 1.51284892769268 1105 30-31 1.2163366581335282 1105 32-33 1.140740827459581 1105 34-35 0.9488197710854038 1105 36-37 0.9437416894448205 1105 38-39 1.1586458535677338 1105 40-41 1.9577062694250102 1105 42-43 0.857563414594658 1105 44-45 -0.9953566977004513 1105 46-47 -0.9639771253052416 1105 48-49 0.1107332193269599 1105 50-51 -3.5352313340594534 1105 52-53 -3.282577491952498 1105 54-55 -3.100288585835461 1105 56-57 -2.4236166611166716 1105 58-59 -2.8469951778545486 1105 60-61 -1.8594564868002443 1105 62-63 -1.066373441373436 1105 64-65 -1.1955564979002489 1105 66-67 0.3217932096838467 1105 68-69 1.9952704269110448 1105 70-71 0.0016730809699438964 1105 72-73 0.8267065255346395 1105 74-75 0.8069931659775413 1105 76-77 0.9298832966801562 1105 78-79 1.0504041531385155 1105 80-81 -0.3416170201326416 1105 82-83 -0.2303241029803509 1105 84-85 3.31181958955397 1105 86-87 1.4141177543521266 1105 88-89 3.0801552324989707 1105 90-91 2.6667647167647175 1105 92-93 4.607685008856883 1105 94-95 4.762741410788287 1105 96-97 4.967273582898578 1105 98-99 1.076989546911431 1105 100-101 3.4656986242923757 1106 1 -0.8858213545713518 1106 2 -0.41861558580307445 1106 3 -3.9557089900839806 1106 4 -1.891780788655801 1106 5 -0.27929050116551224 1106 6 -0.3673899306711874 1106 7 -0.18879722707848856 1106 8 -0.02119495088244605 1106 9 -1.4605889769952398 1106 10-11 -0.8897780893874554 1106 12-13 -1.3202166756854155 1106 14-15 -1.5920681748806658 1106 16-17 -1.0081610085516317 1106 18-19 -0.689071981259481 1106 20-21 -0.7307699100667833 1106 22-23 -0.6448133030164485 1106 24-25 -0.4504478449790952 1106 26-27 -1.0407586770868065 1106 28-29 -1.1538177389739843 1106 30-31 -0.9503300085331432 1106 32-33 -0.27592583920708336 1106 34-35 -0.7178468955812676 1106 36-37 -1.7229249772218367 1106 38-39 -0.008020813098937651 1106 40-41 -0.8756270639083183 1106 42-43 0.7742300812613294 1106 44-45 1.5879766356328844 1106 46-47 2.369356208028094 1106 48-49 3.527399885993624 1106 50-51 1.7147686659405394 1106 52-53 0.9674225080474983 1106 54-55 1.6497114141645355 1106 56-57 4.4097166722166605 1106 58-59 3.4030048221454443 1106 60-61 2.1405435131997557 1106 62-63 2.0169598919598997 1106 64-65 2.6377768354330797 1106 66-67 -3.011540123649489 1106 68-69 -3.838062906422291 1106 70-71 -3.248326919030056 1106 72-73 -5.589960141132025 1106 74-75 -2.1930068340224587 1106 76-77 -4.153450036653176 1106 78-79 -3.7829291801948166 1106 80-81 -1.758283686799306 1106 82-83 -2.8969907696470187 1106 84-85 -2.0215137437793658 1106 86-87 0.9141177543521266 1106 88-89 0.08015523249896717 1106 90-91 2.8334313834313853 1106 92-93 -1.4756483244764453 1106 94-95 -1.4872585892117165 1106 96-97 -1.5327264171014257 1106 98-99 -0.5063437864219047 1106 100-101 0.2156986242923793 1107 1 1.0725119787619803 1107 2 0.49805108086358985 1107 3 -0.03904232341731273 1107 4 0.5248858780108634 1107 5 0.22070949883448776 1107 6 0.3826100693288126 1107 7 0.8112027729215114 1107 8 0.5204717157842182 1107 9 0.6227443563380959 1107 10-11 0.8810552439458803 1107 12-13 0.8464499909812488 1107 14-15 1.2620984917860056 1107 16-17 1.3043389914483683 1107 18-19 0.623428018740519 1107 20-21 0.7900634232665453 1107 22-23 0.646853363650223 1107 24-25 0.4453854883542405 1107 26-27 1.250907989579865 1107 28-29 1.26284892769268 1107 30-31 1.5288366581335282 1107 32-33 1.0574074941262452 1107 34-35 1.8238197710854038 1107 36-37 0.8604083561114919 1107 38-39 2.0336458535677338 1107 40-41 1.5618729360916817 1107 42-43 2.795063414594658 1107 44-45 3.9421433022995487 1107 46-47 4.786022874694758 1107 48-49 5.73573321932696 1107 50-51 3.568935332607211 1107 52-53 2.2590891747141697 1107 54-55 3.378878080831207 1107 56-57 4.618050005549996 1107 58-59 4.319671488812116 1107 60-61 6.411376846533091 1107 62-63 4.3294598919599 1107 64-65 4.283610168766415 1107 66-67 4.696793209683847 1107 68-69 4.495270426911045 1107 70-71 5.605839747636608 1107 72-73 5.972539858867975 1107 74-75 6.036159832644206 1107 76-77 5.825716630013492 1107 78-79 7.1754041531385155 1107 80-81 8.366716313200694 1107 82-83 6.019675897019649 1107 84-85 8.603486256220634 1107 86-87 8.622451087685462 1107 88-89 8.121821899165635 1107 90-91 6.020931383431385 1107 92-93 5.816018342190219 1107 94-95 5.679408077454951 1107 96-97 5.883940249565242 1107 98-99 6.931156213578095 1107 100-101 9.486531957625711 1108 1 0.8919564232064232 1108 2 0.3591621919747041 1108 3 0.21095767658268727 1108 4 -0.0028918997669151736 1108 5 0.2762650543900449 1108 6 -0.9229454862267445 1108 7 -0.29990833818960283 1108 8 -1.7989727286602246 1108 9 -0.4605889769952398 1108 10-11 -2.723111422720784 1108 12-13 -0.2091055645743083 1108 14-15 -0.09206817488065866 1108 16-17 -0.39704989744051744 1108 18-19 -0.8001830923705953 1108 20-21 -0.7863254656223404 1108 22-23 -0.5059244141275556 1108 24-25 -0.2837811783124238 1108 26-27 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-0.7525489123145377 2311 88-89 -0.20317810083436783 2311 90-91 -0.5665686165686132 2311 92-93 -0.5089816578097803 2311 94-95 0.9294080774549514 2311 96-97 1.833940249565245 2311 98-99 1.8436562135780967 2311 100-101 1.5990319576257086 2312 1 1.0030675343175375 2312 2 0.803606636419147 2312 3 0.4331798988049087 2312 4 0.552663655788642 2312 5 0.2762650543900449 2312 6 0.18816562488436972 2312 7 0.03342499514373287 2312 8 0.20102727133977538 2312 9 0.31718880078253875 2312 10-11 0.33244413283476604 2312 12-13 0.17978332431458455 2312 14-15 -1.7031792859917658 2312 16-17 -1.8970498974405174 2312 18-19 0.36648357429607614 2312 20-21 0.3803412010443239 2312 22-23 0.8274089192057801 2312 24-25 0.605107710576462 2312 26-27 -1.6518697881979136 2312 28-29 -0.8482621834184343 2312 30-31 -2.950330008533136 2312 32-33 -2.609259172540426 2312 34-35 -1.4122913400257104 2312 36-37 -1.8618138661107295 2312 38-39 -1.1191319242100377 2312 40-41 -4.15340484168609 2312 42-43 -6.225769918738674 2312 44-45 -9.578690031033787 2312 46-47 -9.74175490308302 2312 48-49 -8.333711225117483 2312 50-51 -8.36856466739279 2312 52-53 -7.643688603063609 2312 54-55 -7.933621919168793 2312 56-57 -7.506949994450004 2312 58-59 -6.958106288965663 2312 60-61 -6.359456486800244 2312 62-63 -6.760817885817879 2312 64-65 -6.640000942344695 2312 66-67 -5.928206790316153 2312 68-69 -5.754729573088955 2312 70-71 -2.748326919030056 2312 72-73 -4.4232934744653605 2312 74-75 -4.526340167355794 2312 76-77 -4.375672258875394 2312 78-79 -6.310706957972595 2312 80-81 -5.091617020132638 2312 82-83 -6.535879658535908 2312 84-85 -5.493735966001587 2312 86-87 -4.752548912314538 2312 88-89 -5.642066989723254 2312 90-91 -5.6110130610130575 2312 92-93 -5.003426102254224 2312 94-95 -6.459480811433938 2312 96-97 -5.254948639323647 2312 98-99 -4.228566008644126 2312 100-101 -4.839856931263178 2313 1 -0.7747102434602411 2313 2 -0.6408378080252959 2313 3 -2.4557089900839806 2313 4 -0.8917807886558009 2313 5 0.05404283216782346 2313 6 -0.03405659733785171 2313 7 0.58898055069929 2313 8 0.20102727133977538 2313 9 0.09496657856031732 2313 10-11 -0.3897780893874483 2313 12-13 -1.9868833423520798 2313 14-15 -1.5365126193251086 2313 16-17 -1.2303832307738531 2313 18-19 -0.30018309237059526 2313 20-21 -2.3418810211778975 2313 22-23 -4.8948133030164485 2313 24-25 -1.0060034005346452 2313 26-27 -0.8740920104201351 2313 28-29 -2.292706627862877 2313 30-31 0.8274477692446425 2313 32-33 0.5018519385706881 2313 34-35 0.25437532664096096 2313 36-37 0.02707502277815621 2313 38-39 -1.4524652575433805 2313 40-41 -1.153404841686097 2313 42-43 -1.7257699187386777 2313 44-45 -0.46757891992267275 2313 46-47 -1.0750882364163559 2313 48-49 -3.88926678067304 2313 50-51 -5.424120222948346 2313 52-53 -2.7547997141747196 2313 54-55 -4.822510808057682 2313 56-57 -9.395838883338893 2313 58-59 -7.235884066743441 2313 60-61 -4.74834537568913 2313 62-63 -6.316373441373436 2313 64-65 -4.751112053455806 2313 66-67 -2.0393179014272675 2313 68-69 -5.032507350866734 2313 70-71 -6.470549141252281 2313 72-73 -3.256626807798689 2313 74-75 -3.804117945133573 2313 76-77 -3.6534500366531724 2313 78-79 -2.421818069083706 2313 80-81 -2.7027281312437488 2313 82-83 -1.6469907696470152 2313 84-85 -3.327069299334923 2313 86-87 -1.6969933567589806 2313 88-89 -2.0309558786121436 2313 90-91 -1.1110130610130575 2313 92-93 -2.558981657809781 2313 94-95 -1.79281414476727 2313 96-97 -3.366059750434758 2313 98-99 -2.839677119755237 2313 100-101 -2.8954124868187314 2314 1 0.28084531209531605 2314 2 -0.0019489191364101544 2314 3 0.12762434324935157 2314 4 0.2748858780108634 2314 5 0.22070949883448776 2314 6 0.4659434026621483 2314 7 -0.9387972270784886 2314 8 0.47880504911755395 2314 9 -0.8772556436619041 2314 10-11 0.151888577279216 2314 12-13 0.22144999098124885 2314 14-15 -0.46706817488066577 2314 16-17 -0.299827675218296 2314 18-19 -0.439071981259481 2314 20-21 -0.23076991006678327 2314 22-23 0.10518669698355865 2314 24-25 -0.4921145116457595 2314 26-27 -0.12409201042013507 2314 28-29 0.26284892769268 2314 30-31 -0.2419966751998075 2314 32-33 -1.109259172540419 2314 34-35 -2.259513562247932 2314 36-37 -2.3062583105551795 2314 38-39 -2.299687479765595 2314 40-41 -1.5422937305749898 2314 42-43 -2.1841032520720134 2314 44-45 -3.4953566977004513 2314 46-47 -5.338977125305242 2314 48-49 -3.9309334473397044 2314 50-51 -1.8685646673927891 2314 52-53 -2.2825774919524946 2314 54-55 -1.6419552525021253 2314 56-57 -2.298616661116668 2314 58-59 -2.4719951778545486 2314 60-61 -2.567789820133573 2314 62-63 -1.6080401080401003 2314 64-65 -4.403889831233585 2314 66-67 -5.303206790316153 2314 68-69 -5.0463962397556195 2314 70-71 -4.998326919030056 2314 72-73 -3.881626807798689 2314 74-75 -4.443006834022462 2314 76-77 -4.070116703319844 2314 78-79 -3.1162625135281488 2314 80-81 -2.79995035346597 2314 82-83 -5.063657436313687 2314 84-85 -4.68818041044603 2314 86-87 -3.4192155789812055 2314 88-89 -3.2948447675010293 2314 90-91 -4.24990194990195 2314 92-93 -4.683981657809781 2314 94-95 -4.278925255878381 2314 96-97 -3.366059750434758 2314 98-99 -1.8813437864219047 2314 100-101 -3.4093013757076243 2315 1 -0.4413769101269054 2315 2 -0.5297266969141887 2315 3 -1.0112645456395342 2315 4 -0.11400301087802234 2315 5 -0.16817939005439797 2315 6 -0.03405659733785171 2315 7 0.3667583284770686 2315 8 0.31213838245088255 2315 9 -0.12725564366190412 2315 10-11 -1.3342225338318983 2315 12-13 -0.2091055645743083 2315 14-15 -0.1476237304362158 2315 16-17 -0.11927211966274598 2315 18-19 -0.46684975903725956 2315 20-21 -0.5085476878445689 2315 22-23 0.4940755858724444 2315 24-25 -1.839336733867981 2315 26-27 -1.5963142326423565 2315 28-29 -2.292706627862877 2315 30-31 -2.283663341866472 2315 32-33 -2.998148061429312 2315 34-35 -0.9678468955812605 2315 36-37 -0.5284805327773938 2315 38-39 -2.230243035321152 2315 40-41 -1.0978492861305469 2315 42-43 -0.8368810298497849 2315 44-45 0.03242108007732725 2315 46-47 -0.7973104586385702 2315 48-49 -5.778155669561929 2315 50-51 -4.9796757785039 2315 52-53 -3.7547997141747196 2315 54-55 -6.266955252502125 2315 56-57 -7.173616661116668 2315 58-59 -8.680328511187884 2315 60-61 -8.970567597911351 2315 62-63 -7.0385956635956575 2315 64-65 -5.473334275678027 2315 66-67 -4.039317901427264 2315 68-69 -3.865840684200066 2315 70-71 -3.081660252363392 2315 72-73 -4.4232934744653605 2315 74-75 -3.6930068340224587 2315 76-77 -3.0978944810976223 2315 78-79 -3.1440402913059273 2315 80-81 -3.7027281312437488 2315 82-83 -4.2581018807581295 2315 84-85 -4.327069299334923 2315 86-87 -4.808104467870095 2315 88-89 -1.5309558786121436 2315 90-91 -3.6110130610130575 2315 92-93 -1.558981657809781 2315 94-95 -3.5705919225450486 2315 96-97 -1.254948639323647 2315 98-99 -2.673010453088569 2315 100-101 -2.9509680423742886 2316 1 -1.3524880212380204 2316 2 -1.1519489191364087 2316 3 -2.1890423234173113 2316 4 -0.42511412198913945 2316 5 -0.27929050116551224 2316 6 0.232610069328814 2316 7 -0.28879722707849 2316 8 0.045471715784216826 2316 9 -2.127255643661904 2316 10-11 -1.5064447560541225 2316 12-13 -1.2035500090187483 2316 14-15 -0.6254015082139972 2316 16-17 -1.8081610085516289 2316 18-19 -0.7890719812594824 2316 20-21 -0.8974365767334547 2316 22-23 -3.478146636349777 2316 24-25 -0.9671145116457538 2316 26-27 -3.1740920104201322 2316 28-29 -1.5871510723073143 2316 30-31 -1.2836633418664718 2316 32-33 -2.1425925058737576 2316 34-35 -0.8511802289145933 2316 36-37 -0.9395916438885052 2316 38-39 -1.8913541464322634 2316 40-41 -0.9256270639083155 2316 42-43 -1.9924365854053363 2316 44-45 -1.7453566977004513 2316 46-47 -3.663977125305241 2316 48-49 -2.4892667806730415 2316 50-51 -3.1685646673927863 2316 52-53 -1.9659108252858317 2316 54-55 -2.0836219191687917 2316 56-57 -3.0569499944500045 2316 58-59 -3.0303285111878857 2316 60-61 -1.5761231534669058 2316 62-63 -2.8330401080401018 2316 64-65 -6.428889831233583 2316 66-67 -6.978206790316154 2316 68-69 -6.704729573088958 2316 70-71 -6.781660252363391 2316 72-73 -6.189960141132026 2316 74-75 -6.726340167355794 2316 76-77 -6.686783369986511 2316 78-79 -4.549595846861486 2316 80-81 -4.058283686799303 2316 82-83 -7.580324102980352 2316 84-85 -8.021513743779366 2316 86-87 -5.152548912314536 2316 88-89 -4.853178100834366 2316 90-91 -5.516568616568616 2316 92-93 -5.108981657809782 2316 94-95 -2.7205919225450472 2316 96-97 -0.6160597504347578 2316 98-99 -2.2063437864219075 2316 100-101 -3.2509680423742857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 2.0 15 8.0 16 36.0 17 70.0 18 90.0 19 158.0 20 214.0 21 322.0 22 359.0 23 279.0 24 211.0 25 231.0 26 236.0 27 222.0 28 208.0 29 182.0 30 127.0 31 91.0 32 122.0 33 144.0 34 201.0 35 300.0 36 574.0 37 1371.0 38 1720.0 39 378.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.0 18.535031847133755 14.70063694267516 20.764331210191084 2 16.721812165945533 20.475948078391447 39.501145329600405 23.301094426062612 3 18.414354797658437 28.55688470348689 33.698142020870456 19.33061847798422 4 10.244336981420208 20.9086281496564 33.68541613642148 35.16161873250191 5 10.715194706032069 37.33774497327565 33.13820310511581 18.808857215576484 6 27.16976329854925 37.52863324001018 15.894629676762534 19.406973784678037 7 21.71035886994146 39.4629676762535 17.841689997454825 20.984983456350218 8 26.698905573937388 31.572919317892595 20.272333927207942 21.455841180962075 9 27.05523033850853 12.382285568847035 18.159837108679053 42.40264698396538 10-11 23.606515652837874 26.66072792059048 24.7518452532451 24.980911173326543 12-13 27.65334690761008 26.196233138203105 25.229065920081446 20.92135403410537 14-15 25.19725120895902 25.330872995673197 26.845253245100537 22.626622550267246 16-17 19.426062611351487 30.828455077627897 26.488928480529395 23.25655383049122 18-19 19.947823873759226 24.40824637312293 34.455332145584116 21.188597607533723 20-21 20.29778569610588 24.160091626368033 35.906082972766605 19.63603970475948 22-23 19.928735047085773 26.240773733774496 35.30160346144057 18.52888775769916 24-25 21.366759989819293 26.374395520488676 35.543395265970986 16.71544922372105 26-27 19.712395011453296 28.105115805548486 35.63247645711377 16.55001272588445 28-29 18.77704250445406 26.14532960040723 36.38330363960296 18.69432425553576 30-31 17.638075846271317 27.01705268516162 37.318656146602194 18.026215321964877 32-33 18.923390175617204 27.112496818528886 37.0259608042759 16.93815220157801 34-35 18.630694833290914 27.303385085263425 37.13413082209214 16.931789259353526 36-37 17.211758717230847 27.589717485365234 35.2888775769916 19.90964622041232 38-39 18.662509544413336 25.63629422244846 35.81700178162382 19.884194451514382 40-41 16.98905573937389 26.075337235937894 36.82870959531687 20.106897429371344 42-43 18.681598371086793 25.0 36.803257826418935 19.515143802494272 44-45 16.95087808602698 26.45075082718249 36.37057775515398 20.227793331636548 46-47 18.796131331127512 25.10817001781624 33.634512598625605 22.461186052430644 48-49 18.891575464494785 25.184525324510055 33.284550776278955 22.639348434716215 50-51 19.177907864596587 26.62255026724357 32.5209977093408 21.678544158819037 52-53 18.414354797658437 28.40417409009926 32.501908882667344 20.679562229574955 54-55 17.90531941969967 29.600407228302366 29.969457877322476 22.52481547467549 56-57 18.490710104352253 30.535759735301603 27.914227538813947 23.059302621532197 58-59 17.020870450496307 30.758462713158565 30.14125731738356 22.07940951896157 60-61 18.745227793331637 31.490201068974294 27.48791040977348 22.276660727920593 62-63 16.893611606006615 31.738355815729193 28.563247645711375 22.804784932552813 64-65 16.874522779333166 31.261135148892848 28.91320946805803 22.95113260371596 66-67 16.791804530414865 31.47111224230084 29.434970730465764 22.30211249681853 68-69 17.994400610842455 30.73937388648511 28.321455841180963 22.944769661491474 70-71 17.8925935352507 30.446678544158818 28.601425299058285 23.059302621532197 72-73 18.007126495291423 29.212267752608806 28.607788241282766 24.172817510817 74-75 17.8098752863324 29.11682361924154 27.933316365487403 25.139984728938664 76-77 18.439806566556374 28.321455841180963 28.296004072283026 24.942733519979637 78-79 18.643420717739883 27.95876813438534 28.194196996691268 25.20361415118351 80-81 18.974293713413083 27.94604224993637 27.246118605243062 25.833545431407483 82-83 19.15245609569865 27.977856961058794 26.310766098243825 26.558920844998728 84-85 19.146093153474165 26.985237974039194 26.41257317383558 27.456095698651055 86-87 19.52786968694324 27.456095698651055 25.661745991346397 27.354288623059304 88-89 19.95418681598371 27.392466276406207 24.86001527106134 27.79333163654874 90-91 21.080427589717484 26.38075846271316 24.936370577755156 27.6024433698142 92-93 20.234156273861036 26.79434970730466 24.879104097734793 28.092389921099514 94-95 20.749554594044287 26.03715958259099 25.77627895138712 27.437006871977605 96-97 20.953168745227792 26.068974293713413 25.661745991346397 27.316110969712394 98-99 20.844998727411554 26.959786205141256 25.114532960040727 27.080682107406467 100-101 21.26495291422754 26.82616441842708 25.06999236446933 26.838890302876052 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 5.5 2 4.0 3 2.5 4 0.5 5 2.0 6 6.0 7 13.5 8 22.5 9 26.0 10 22.0 11 17.5 12 18.5 13 22.5 14 25.0 15 21.5 16 23.0 17 28.0 18 30.5 19 36.5 20 36.5 21 33.5 22 38.5 23 46.5 24 51.5 25 53.0 26 57.0 27 81.0 28 91.0 29 94.5 30 123.0 31 140.5 32 150.0 33 167.5 34 187.0 35 191.5 36 197.0 37 222.0 38 240.0 39 257.0 40 269.0 41 262.5 42 238.0 43 240.0 44 261.5 45 248.5 46 243.0 47 265.0 48 277.5 49 289.5 50 312.0 51 312.5 52 259.0 53 224.0 54 216.5 55 184.5 56 162.5 57 134.0 58 107.0 59 106.5 60 95.5 61 77.0 62 66.5 63 48.5 64 38.0 65 32.5 66 23.0 67 16.5 68 17.0 69 15.0 70 7.0 71 2.0 72 3.5 73 5.0 74 3.5 75 2.5 76 1.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10180707559175363 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7858.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.73301094426063 #Duplication Level Percentage of deduplicated Percentage of total 1 96.7843631778058 78.13693051667092 2 1.8442622950819672 2.9778569610587935 3 0.3467843631778058 0.8399083736319674 4 0.26796973518284994 0.8653601425299059 5 0.15762925598991173 0.6362942224484602 6 0.12610340479192939 0.6108424535505218 7 0.031525851197982346 0.17816238228556885 8 0.04728877679697352 0.3054212267752609 9 0.031525851197982346 0.22906592008144566 >10 0.23644388398486757 2.9778569610587935 >50 0.06305170239596469 3.9959277169763303 >100 0.06305170239596469 8.246373122932043 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 182 2.316110969712395 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 175 2.2270297785696105 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 2.0488673962840416 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 130 1.6543649783659964 TruSeq Adapter, Index 3 (96% over 28bp) ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 98 1.247136675998982 TruSeq Adapter, Index 3 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 1.0817001781623823 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 76 0.9671672181216595 RNA PCR Primer, Index 3 (95% over 23bp) CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA 55 0.6999236446933061 RNA PCR Primer, Index 3 (96% over 28bp) CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT 50 0.6362942224484602 TruSeq Adapter, Index 3 (96% over 26bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.27996945787732247 No Hit CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG 21 0.26724357342835325 TruSeq Adapter, Index 3 (95% over 24bp) ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC 17 0.21634003563247647 Illumina PCR Primer Index 12 (95% over 24bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.21634003563247647 No Hit TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT 12 0.15271061338763045 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 11 0.13998472893866123 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.13998472893866123 No Hit GTACATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA 11 0.13998472893866123 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.13998472893866123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 11 0.13998472893866123 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 10 0.12725884448969205 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 10 0.12725884448969205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 10 0.12725884448969205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 10 0.12725884448969205 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.11453296004072283 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.11453296004072283 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 8 0.10180707559175363 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 8 0.10180707559175363 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 8 0.10180707559175363 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.006362942224484602 0.0 22-23 0.0 0.0 0.0 0.05090353779587682 0.0 24-25 0.0 0.0 0.0 0.13998472893866123 0.0 26-27 0.0 0.0 0.0 0.21634003563247645 0.0 28-29 0.0 0.0 0.0 0.3499618223466531 0.0 30-31 0.0 0.0 0.0 0.6617459913463986 0.0 32-33 0.0 0.0 0.0 0.9035377958768134 0.0 34-35 0.0 0.0 0.0 1.1071519470603208 0.0 36-37 0.0 0.0 0.0 1.5589208449987275 0.0 38-39 0.0 0.0 0.0 2.15703741410028 0.0 40-41 0.0 0.0 0.0 2.8824128276915246 0.0 42-43 0.0 0.0 0.0 3.321455841180962 0.0 44-45 0.0 0.0 0.0 3.690506490201069 0.0 46-47 0.0 0.0 0.0 3.9704759480783913 0.0 48-49 0.0 0.0 0.0 4.403156019343344 0.0 50-51 0.0 0.0 0.0 4.753117841689997 0.0 52-53 0.0 0.0 0.0 5.020361415118351 0.0 54-55 0.0 0.0 0.0 5.192160855179435 0.0 56-57 0.0 0.0 0.0 5.542122677526088 0.0 58-59 0.0 0.0 0.0 5.853906846525834 0.0 60-61 0.0 0.0 0.0 6.203868668872486 0.0 62-63 0.0 0.0 0.0 6.528378722321202 0.0 64-65 0.0 0.0 0.0 6.776533469076101 0.0 66-67 0.0 0.0 0.0 7.101043522524815 0.0 68-69 0.0 0.0 0.0 7.419190633749046 0.0 70-71 0.0 0.0 0.0 7.762789513871214 0.0 72-73 0.0 0.0 0.0 8.010944260626113 0.0 74-75 0.0 0.0 0.0 8.31000254517689 0.0 76-77 0.0 0.0 0.0 8.577246118605242 0.0 78-79 0.0 0.0 0.0 8.901756172053958 0.0 80-81 0.0 0.0 0.0 9.168999745482312 0.0 82-83 0.0 0.0 0.0 9.391702723339272 0.0 84-85 0.0 0.0 0.0 9.614405701196233 0.0 86-87 0.0 0.0 0.0 9.907101043522523 0.0 88-89 0.0 0.0 0.0 10.352506999236446 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 110 1.1514203E-9 39.11014 1 AACGCAG 150 6.82121E-10 31.664543 6 GTATCAA 160 1.2714736E-9 29.875801 1 TATCAAC 180 1.4188117E-10 29.025833 2 TCAACGC 165 1.897206E-9 28.78595 4 GCAGAGT 165 1.897206E-9 28.78595 9 CAACGCA 165 1.897206E-9 28.78595 5 GAGTACT 125 0.0 28.498089 12-13 ACGCAGA 170 2.6102498E-9 27.939302 7 CGCAGAG 170 2.6102498E-9 27.939302 8 ATCAACG 170 2.6102498E-9 27.939302 3 GTACTTT 130 0.0 27.402008 14-15 ACTTTTT 140 0.0 25.444723 16-17 CAGAGTA 160 0.0 25.232683 10-11 CTTATAC 95 0.0010801415 25.158567 1 TATACAC 95 0.001121839 24.998323 3 ATACACA 95 0.001121839 24.998323 4 TTATACA 100 0.0015129657 23.748407 2 TACACAT 100 0.0015129657 23.748407 5 ACACATC 105 0.0020100446 22.61753 6 >>END_MODULE