FastQCFastQC Report
Fri 10 Feb 2017
SRR2031839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094643
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20660.18873733262808057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.17211090739172497No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.052.4707571
GTATCAA29850.039.7821121
TCAACGC32550.035.1688544
ATCAACG33700.033.968733
CAACGCA33800.033.5871625
AACGCAG34400.033.0013436
TATCAAC35200.032.656142
ACGCAGA37950.029.6639337
CGCAGAG38450.028.7840378
GCAGAGT44900.023.8028149
GTACATG30800.023.749921
GAGTACT22500.023.1165912-13
TACATGG31700.022.775952
CAGAGTA40450.022.25270510-11
ACATGGG33200.021.0315553
GTACTTT26200.019.85203414-15
AGAGTAC36800.019.68403610-11
TCGCCGG757.7242835E-418.99993794-95
GTCTACG1250.00603108218.9999351
CGACGGA650.007565404818.26916932-33
Produced by FastQC (version 0.11.5)