##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031839_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1094643 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46486571421002 34.0 31.0 34.0 31.0 34.0 2 32.602649448267606 34.0 31.0 34.0 31.0 34.0 3 32.67897478904081 34.0 31.0 34.0 31.0 34.0 4 35.88778350567262 37.0 37.0 37.0 35.0 37.0 5 35.9071194900986 37.0 37.0 37.0 35.0 37.0 6 35.979593346871994 37.0 37.0 37.0 35.0 37.0 7 36.003634061515946 37.0 37.0 37.0 35.0 37.0 8 35.99872013067274 37.0 37.0 37.0 35.0 37.0 9 37.82150801676894 39.0 39.0 39.0 35.0 39.0 10-11 37.84999355954407 39.0 39.0 39.0 35.0 39.0 12-13 37.81927486861014 39.0 39.0 39.0 35.0 39.0 14-15 39.38062592096236 41.0 40.0 41.0 37.0 41.0 16-17 39.31075747983589 41.0 40.0 41.0 36.5 41.0 18-19 39.30186599649383 41.0 40.0 41.0 36.5 41.0 20-21 39.235165711560754 41.0 40.0 41.0 36.0 41.0 22-23 39.14403234661894 41.0 39.0 41.0 36.0 41.0 24-25 39.10303496208353 41.0 39.0 41.0 36.0 41.0 26-27 38.97367497896575 41.0 39.0 41.0 35.5 41.0 28-29 38.85326129158091 41.0 39.0 41.0 35.0 41.0 30-31 38.71492258206557 40.5 39.0 41.0 35.0 41.0 32-33 38.60698967608617 40.0 38.0 41.0 35.0 41.0 34-35 38.42114141322787 40.0 38.0 41.0 34.0 41.0 36-37 38.30341033560713 40.0 38.0 41.0 34.0 41.0 38-39 38.116660408918705 40.0 38.0 41.0 33.5 41.0 40-41 38.014443521769195 40.0 38.0 41.0 33.0 41.0 42-43 37.8579719598079 40.0 38.0 41.0 33.0 41.0 44-45 38.0487263884207 40.0 38.0 41.0 33.0 41.0 46-47 37.94951139321222 40.0 38.0 41.0 33.0 41.0 48-49 37.80424120009903 40.0 38.0 41.0 33.0 41.0 50-51 36.74228903852672 39.0 36.5 40.0 31.5 40.5 52-53 36.75399513814093 39.5 37.0 40.0 31.5 40.5 54-55 37.543648477174756 40.0 37.0 41.0 32.5 41.0 56-57 37.527022051938395 40.0 37.0 41.0 32.5 41.0 58-59 37.385324256401404 40.0 37.0 41.0 32.0 41.0 60-61 37.16310523156865 40.0 36.0 41.0 32.0 41.0 62-63 36.869476623885596 39.0 35.5 41.0 31.5 41.0 64-65 36.52883862592644 39.0 35.0 41.0 31.0 41.0 66-67 36.23569191051329 38.5 35.0 41.0 31.0 41.0 68-69 35.87424301804333 37.0 35.0 40.0 30.5 41.0 70-71 35.48984372073818 37.0 35.0 39.5 30.0 41.0 72-73 35.06189826272127 36.5 35.0 39.0 30.0 41.0 74-75 34.686386337828864 36.0 35.0 39.0 29.0 40.5 76-77 34.29868231012303 35.0 35.0 37.0 29.0 39.0 78-79 33.92829351669906 35.0 34.0 37.0 29.0 39.0 80-81 33.592493169005785 35.0 34.0 36.5 29.0 38.5 82-83 33.33066488343688 35.0 34.0 36.0 28.5 37.0 84-85 33.05614113459822 35.0 34.0 36.0 27.5 37.0 86-87 32.83001124567553 35.0 34.0 35.0 27.0 36.5 88-89 32.66144532966456 35.0 34.0 35.0 27.0 36.0 90-91 32.51341213528063 35.0 34.0 35.0 26.0 36.0 92-93 32.41056125147651 35.0 34.0 35.0 26.0 36.0 94-95 32.266721204995605 35.0 34.0 35.0 25.5 35.0 96-97 32.18766666392605 35.0 34.0 35.0 25.0 35.0 98-99 32.08669082066025 35.0 33.0 35.0 25.0 35.0 100-101 30.488649724156645 33.5 30.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2286204278729329 1101 2 0.2795135147141252 1101 3 0.28136697114263143 1101 4 0.22075920989233566 1101 5 0.23499671679298473 1101 6 0.17074244788101822 1101 7 0.17233955462562278 1101 8 0.23862243440531472 1101 9 0.29501014905837053 1101 10-11 0.2991239608310252 1101 12-13 0.3510201156382493 1101 14-15 0.30793499894186027 1101 16-17 0.3757066015440955 1101 18-19 0.36633208158222175 1101 20-21 0.4129856087361503 1101 22-23 0.3461810541943109 1101 24-25 0.4730658925195641 1101 26-27 0.29100712144541774 1101 28-29 0.4559258630679821 1101 30-31 0.38700522392881 1101 32-33 0.3043592189129285 1101 34-35 0.6343769081438495 1101 36-37 0.616741587463217 1101 38-39 0.42732611239397755 1101 40-41 0.30529447720443414 1101 42-43 0.3112347556432269 1101 44-45 0.5081515332552797 1101 46-47 0.6339653945352453 1101 48-49 0.47689983255943247 1101 50-51 0.5251939908934489 1101 52-53 0.45128681112548463 1101 54-55 0.5654881249501287 1101 56-57 0.5650409068653701 1101 58-59 0.7566664918418198 1101 60-61 0.625848219296337 1101 62-63 0.5139353397279507 1101 64-65 0.6679793880793099 1101 66-67 0.5350646426955237 1101 68-69 0.5208204617886736 1101 70-71 0.5405781384268877 1101 72-73 0.5702963946144664 1101 74-75 0.5475311982399376 1101 76-77 0.5622178775787319 1101 78-79 0.4948306577199162 1101 80-81 0.457741519012707 1101 82-83 0.3899418456709327 1101 84-85 0.4042165403389717 1101 86-87 0.36693661675097644 1101 88-89 0.35118937385734483 1101 90-91 0.31279645358703334 1101 92-93 0.36755941156194183 1101 94-95 0.48168280798752505 1101 96-97 0.4357132587034087 1101 98-99 0.3257769657053444 1101 100-101 0.37143232612677224 1102 1 0.044519520699367376 1102 2 0.059229642686318584 1102 3 0.01604020509379467 1102 4 -0.004907114032938864 1102 5 0.0066950068086839565 1102 6 0.03638268751758744 1102 7 0.056112996147135163 1102 8 0.11879131477059701 1102 9 0.16912008047157912 1102 10-11 0.1532290912973977 1102 12-13 0.26329629074700733 1102 14-15 0.25864698962751476 1102 16-17 0.34113657511321804 1102 18-19 0.29572671299401776 1102 20-21 0.4846121412843871 1102 22-23 0.39316550759880187 1102 24-25 0.308274034103853 1102 26-27 0.38494457820159056 1102 28-29 0.3596405635928832 1102 30-31 0.28556725049319454 1102 32-33 0.2371870407849599 1102 34-35 0.3870100477705307 1102 36-37 0.4235227323221622 1102 38-39 0.4785229473633521 1102 40-41 0.36474021174289106 1102 42-43 0.4385798705514503 1102 44-45 0.3621591043741219 1102 46-47 0.5669633034276487 1102 48-49 0.4301875183875197 1102 50-51 0.5118521082804435 1102 52-53 0.5726108455125853 1102 54-55 0.48924998771749983 1102 56-57 0.39631201530644233 1102 58-59 0.32832511628069483 1102 60-61 0.30072399158768803 1102 62-63 0.3495542575123807 1102 64-65 0.3947496730424689 1102 66-67 0.20175762471251346 1102 68-69 0.2572626007516874 1102 70-71 0.3317844469223772 1102 72-73 0.34024310923925327 1102 74-75 0.3513746514300635 1102 76-77 0.18613493672922488 1102 78-79 0.1886342271083592 1102 80-81 0.21064294684510543 1102 82-83 0.26432712550580106 1102 84-85 0.31687268343286945 1102 86-87 0.2906118314136279 1102 88-89 0.3014456596959718 1102 90-91 0.5011886883764376 1102 92-93 0.3907881638420676 1102 94-95 0.3503807629638871 1102 96-97 0.2693168057558992 1102 98-99 0.29025701931163894 1102 100-101 0.3513351005349037 1103 1 0.3197656573004082 1103 2 0.2961280959385846 1103 3 0.416667649541715 1103 4 0.21687089973057994 1103 5 0.2978562995941161 1103 6 0.3239313161974877 1103 7 0.3163358638597771 1103 8 0.296474698250897 1103 9 0.4219857455869871 1103 10-11 0.4245762575597638 1103 12-13 0.42228821517604587 1103 14-15 0.5144086856709364 1103 16-17 0.5661767447453201 1103 18-19 0.587557788846091 1103 20-21 0.5983815623905286 1103 22-23 0.602179468931844 1103 24-25 0.4911681910274339 1103 26-27 0.5476515429043758 1103 28-29 0.550882716199304 1103 30-31 0.6643556025145188 1103 32-33 0.6557788999708833 1103 34-35 0.6208181288941006 1103 36-37 0.5396207774579054 1103 38-39 0.48023731438365047 1103 40-41 0.4526171382367963 1103 42-43 0.5564044290325327 1103 44-45 0.6014850874456101 1103 46-47 0.6507885629252144 1103 48-49 0.7153051468696177 1103 50-51 0.5665470099472714 1103 52-53 0.6440121496808544 1103 54-55 0.5791327930327341 1103 56-57 0.6592088097177466 1103 58-59 0.6936345178833108 1103 60-61 0.7422324957504074 1103 62-63 0.6289214686814475 1103 64-65 0.6781026140147972 1103 66-67 0.606148694734344 1103 68-69 0.5950345482934551 1103 70-71 0.5272604614007932 1103 72-73 0.530659311742788 1103 74-75 0.6321022510039 1103 76-77 0.5270770751070941 1103 78-79 0.435636687606781 1103 80-81 0.43918646365735725 1103 82-83 0.5018832154996602 1103 84-85 0.6081884080652671 1103 86-87 0.5476037766649355 1103 88-89 0.6475898955706612 1103 90-91 0.5052125923441295 1103 92-93 0.45301205184601656 1103 94-95 0.686866935240424 1103 96-97 0.5565999504741583 1103 98-99 0.5785465738191391 1103 100-101 0.7365696807752613 1104 1 0.16563010581688076 1104 2 0.17281516197069635 1104 3 0.21174227784430855 1104 4 0.2403916783081428 1104 5 0.12504468511323807 1104 6 0.10914215064599375 1104 7 0.05410079085743291 1104 8 0.24048608852060482 1104 9 0.24980525987456303 1104 10-11 0.2424308904193211 1104 12-13 0.37853868985975936 1104 14-15 0.44168660589839703 1104 16-17 0.5223838961830651 1104 18-19 0.4494427458384038 1104 20-21 0.4771861189262978 1104 22-23 0.487317242202586 1104 24-25 0.5412584210471252 1104 26-27 0.4502533979950698 1104 28-29 0.6044323876379494 1104 30-31 0.5354304362013877 1104 32-33 0.5640528911543328 1104 34-35 0.4628471330049919 1104 36-37 0.578473171414899 1104 38-39 0.5100747291946348 1104 40-41 0.5649109917913862 1104 42-43 0.5377850606533627 1104 44-45 0.6301271015807615 1104 46-47 0.6102738399746954 1104 48-49 0.679905776072907 1104 50-51 0.6135596849384157 1104 52-53 0.7279854368883036 1104 54-55 0.7097705646988999 1104 56-57 0.7611596394381337 1104 58-59 0.6831738259736397 1104 60-61 0.6453376492912 1104 62-63 0.6139583683202119 1104 64-65 0.664906139350343 1104 66-67 0.689928364472145 1104 68-69 0.7049049743481532 1104 70-71 0.6610105536632673 1104 72-73 0.6926470678446819 1104 74-75 0.667266820504544 1104 76-77 0.5615276133315703 1104 78-79 0.5422541262295013 1104 80-81 0.5842931297677723 1104 82-83 0.619260329914745 1104 84-85 0.5874499446177808 1104 86-87 0.6554219645123922 1104 88-89 0.5381494928463226 1104 90-91 0.5785315701722524 1104 92-93 0.5521490099978905 1104 94-95 0.6632199309091646 1104 96-97 0.7110467875028021 1104 98-99 0.4560054354553884 1104 100-101 0.6532654970333489 1105 1 0.14931742681611126 1105 2 0.04537560502321725 1105 3 0.17079369615999695 1105 4 0.1547730976031545 1105 5 0.1372612068471568 1105 6 0.21850924987912634 1105 7 0.18307628186666847 1105 8 0.2511816657899786 1105 9 0.3538380611105083 1105 10-11 0.220659999417677 1105 12-13 0.31187295587914576 1105 14-15 0.341196845791913 1105 16-17 0.32952274182419217 1105 18-19 0.36273292245582667 1105 20-21 0.4648306935914803 1105 22-23 0.5650987618279615 1105 24-25 0.3261691929714132 1105 26-27 0.3745259528420064 1105 28-29 0.500625929320023 1105 30-31 0.4680871724264506 1105 32-33 0.42165971202064867 1105 34-35 0.6405373160179835 1105 36-37 0.6109726167998062 1105 38-39 0.6026624733002279 1105 40-41 0.5038138931778278 1105 42-43 0.5233650681281787 1105 44-45 0.6083886880397529 1105 46-47 0.8308032925346183 1105 48-49 0.8485032694125252 1105 50-51 0.5456513133483085 1105 52-53 0.7437654855345457 1105 54-55 0.7055275093792943 1105 56-57 0.7845643965367017 1105 58-59 0.6645962188329904 1105 60-61 0.42840969728988654 1105 62-63 0.563920094881972 1105 64-65 0.8138272880731208 1105 66-67 0.8614843046496645 1105 68-69 0.6518762762646304 1105 70-71 0.6340542613301423 1105 72-73 0.6391801738691001 1105 74-75 0.6143890176997004 1105 76-77 0.4858641237933128 1105 78-79 0.6653282128134137 1105 80-81 0.6386084349459793 1105 82-83 0.5800565373467919 1105 84-85 0.6332726436646468 1105 86-87 0.7373451098495281 1105 88-89 0.5652791648539477 1105 90-91 0.6833763079242487 1105 92-93 0.5768688544258609 1105 94-95 0.5900037429436154 1105 96-97 0.4187691281983561 1105 98-99 0.6206271879591512 1105 100-101 0.4520351508935754 1106 1 0.33043271259603557 1106 2 0.27004947346631525 1106 3 0.3394640777986808 1106 4 0.395487400875723 1106 5 0.3713375345840788 1106 6 0.40898539533408496 1106 7 0.34291241718644727 1106 8 0.3432974034930396 1106 9 0.3493708293193194 1106 10-11 0.34179525301462377 1106 12-13 0.413490424693606 1106 14-15 0.40147705704233516 1106 16-17 0.44477826748264704 1106 18-19 0.41071997362903545 1106 20-21 0.5187409632137374 1106 22-23 0.4551618419771941 1106 24-25 0.4790473193245788 1106 26-27 0.5288435205501116 1106 28-29 0.36441317752414903 1106 30-31 0.4322651734134979 1106 32-33 0.6241780016712326 1106 34-35 0.6714340147100515 1106 36-37 0.6391421768460148 1106 38-39 0.6071939954113716 1106 40-41 0.5667252803171152 1106 42-43 0.5960263659228318 1106 44-45 0.5206877098814431 1106 46-47 0.635970059733701 1106 48-49 0.7984924913294762 1106 50-51 0.6020013060683667 1106 52-53 0.7395374515514419 1106 54-55 0.9280467066579234 1106 56-57 0.8337502730879507 1106 58-59 0.8018848880422809 1106 60-61 0.8968946769911668 1106 62-63 0.8671803704605381 1106 64-65 0.9013476860680285 1106 66-67 0.9218150972547576 1106 68-69 0.9447768285941152 1106 70-71 0.8954605931703057 1106 72-73 0.9127751940165538 1106 74-75 0.8547284516801525 1106 76-77 0.8473402295395402 1106 78-79 0.822873060038809 1106 80-81 0.855100414728561 1106 82-83 0.8072727097658614 1106 84-85 0.9466761353853812 1106 86-87 0.9931856017839351 1106 88-89 0.8928066726018784 1106 90-91 0.9377915401919381 1106 92-93 0.7998522656324241 1106 94-95 0.8751020031340957 1106 96-97 0.9107947649088857 1106 98-99 0.830342211661474 1106 100-101 0.6977659900146129 1107 1 0.12555332543730913 1107 2 0.17719827212844308 1107 3 0.2027532655401103 1107 4 0.16032741847008225 1107 5 0.19116603309683455 1107 6 0.18221147854148256 1107 7 0.07680900239934374 1107 8 0.13980988721607446 1107 9 0.22964902929406605 1107 10-11 0.207140455284474 1107 12-13 0.14242705450211446 1107 14-15 0.13503046507913297 1107 16-17 0.1796538657563076 1107 18-19 0.22858913950816628 1107 20-21 0.11348878460482581 1107 22-23 0.1264420931759176 1107 24-25 0.2220154534946559 1107 26-27 0.1257531839490511 1107 28-29 0.1966535409087058 1107 30-31 0.1679769394258841 1107 32-33 0.20027362093618706 1107 34-35 0.28849444960437864 1107 36-37 0.37080157785377565 1107 38-39 0.4256798349978155 1107 40-41 0.29626159917574313 1107 42-43 0.2193388966803056 1107 44-45 0.2732861446799646 1107 46-47 0.2757263516781805 1107 48-49 0.4090883043937268 1107 50-51 0.46470517639269104 1107 52-53 0.4827577425552292 1107 54-55 0.46140675090513383 1107 56-57 0.4390595027248665 1107 58-59 0.4158754550249739 1107 60-61 0.5360917817603692 1107 62-63 0.4949524624912556 1107 64-65 0.47485858891143096 1107 66-67 0.47839795842914157 1107 68-69 0.4966579882542419 1107 70-71 0.3859526691744648 1107 72-73 0.5507110791131637 1107 74-75 0.4793104766867984 1107 76-77 0.4028177814842806 1107 78-79 0.3497475782624804 1107 80-81 0.21541772296524897 1107 82-83 0.40213158468474575 1107 84-85 0.4574692317754838 1107 86-87 0.5191459970473886 1107 88-89 0.3913594927082258 1107 90-91 0.38658062123488435 1107 92-93 0.310833064259711 1107 94-95 0.35610531731977346 1107 96-97 0.38512006028949486 1107 98-99 0.3841596222416115 1107 100-101 0.4128775443720478 1108 1 0.13101793288062424 1108 2 0.13613678495447346 1108 3 0.07494134200822344 1108 4 0.08085319874422225 1108 5 0.14229311872225736 1108 6 0.06271393124524849 1108 7 0.10552342165038198 1108 8 0.21208647609660147 1108 9 0.10356599307014847 1108 10-11 0.0652157652847194 1108 12-13 0.1220332156278019 1108 14-15 0.1129155974754994 1108 16-17 0.1523022894908692 1108 18-19 0.09326524198403519 1108 20-21 0.047683942065930296 1108 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-0.8043483680802979 2316 54-55 -0.7122062285901976 2316 56-57 -0.5688080933780242 2316 58-59 -0.6502490405112837 2316 60-61 -0.644891463928758 2316 62-63 -0.8992467140608653 2316 64-65 -0.6501261243085779 2316 66-67 -0.4494892047521404 2316 68-69 -0.5764984260786434 2316 70-71 -0.7508415911492037 2316 72-73 -0.5950962666941777 2316 74-75 -1.0585844317963478 2316 76-77 -1.1432382292776069 2316 78-79 -0.6978085751760261 2316 80-81 -0.6127710993412236 2316 82-83 -0.6637483223716316 2316 84-85 -0.6555387988796113 2316 86-87 -0.5719224469622333 2316 88-89 -0.6138298595399476 2316 90-91 -0.5644405356686306 2316 92-93 -0.8352718641981589 2316 94-95 -0.9237650507858888 2316 96-97 -0.8827613958272522 2316 98-99 -0.9116497618030657 2316 100-101 -0.6818923386331299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 251.0 8 781.0 9 628.0 10 720.0 11 974.0 12 1222.0 13 1406.0 14 1483.0 15 1575.0 16 2088.0 17 2713.0 18 3517.0 19 4297.0 20 5444.0 21 6245.0 22 6638.0 23 6503.0 24 6109.0 25 6263.0 26 6379.0 27 6944.0 28 7994.0 29 9589.0 30 11209.0 31 13925.0 32 17691.0 33 23938.0 34 35351.0 35 59084.0 36 114052.0 37 259849.0 38 381698.0 39 88051.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62234993509299 18.510875235122317 13.508331026645218 24.35844380313947 2 15.820500382316427 20.579129451337103 38.79995578467135 24.80041438167512 3 18.248780652687678 24.566091410624285 30.883584876530524 26.301543060157513 4 12.931247904567975 17.41353116952285 37.53059216566497 32.12462876024421 5 13.79116296363289 37.58841923805296 34.96482414814693 13.655593650167225 6 31.781229131324096 36.74631820602699 17.503149428626504 13.969303234022417 7 27.265784369881324 32.86706259483685 21.864936787610205 18.002216247671615 8 26.199409305134186 34.02378675056617 20.639514435299912 19.13728950899974 9 26.559069943351393 14.892800666518674 20.027260029068838 38.520869361061095 10-11 24.475513934680073 25.628857992971227 29.33600269676963 20.55962537557907 12-13 25.323598652711432 23.860518908904545 28.39062598491015 22.42525645347387 14-15 22.795514153929638 25.54394446408555 25.89821521719867 25.762326164786142 16-17 22.39392203668228 28.31091963315894 26.642704516449655 22.65245381370913 18-19 22.390404908266895 27.77380387943832 28.04832260380782 21.787468608486968 20-21 23.403383568889584 27.036485867995317 27.787232915206143 21.772897647908955 22-23 23.110594047557058 26.929144935837527 27.417660369636494 22.54260064696892 24-25 22.93419620414191 26.97387863869462 27.538264845855785 22.553660311307684 26-27 22.890156882198124 27.104133493750933 27.46224111422628 22.543468509824667 28-29 22.826391800797154 27.058136762396508 27.621836525698335 22.493634911108003 30-31 23.166274301301886 26.91288392654043 27.47900457043986 22.44183720171782 32-33 22.909523926978935 27.16333087591114 27.336126938188983 22.591018258920943 34-35 23.147729442384414 27.231617979560458 27.16862940703042 22.45202317102471 36-37 23.20583057672684 27.00569957511262 27.201653872541094 22.586815975619448 38-39 23.078848537833796 27.085223218894193 27.184387969411034 22.651540273860977 40-41 23.18609811600677 27.010038889391335 27.27788877286933 22.525974221732564 42-43 23.08291379015807 27.01953970381211 27.09997688744184 22.79756961858798 44-45 23.144075282991807 27.1630568139567 27.031004628906413 22.661863274145087 46-47 23.142933358181615 26.978110671698445 26.927135148171594 22.951820821948342 48-49 23.02823843024621 27.04324606287164 26.91402585135062 23.014489655531527 50-51 23.130874632186018 27.06996710343007 26.841856203346666 22.95730206103725 52-53 23.097667458705715 27.11701440560986 26.81138964941081 22.973928486273607 54-55 23.02330531506619 27.038084562729587 26.888720797556832 23.049889324647395 56-57 23.042398297892554 27.103402661872412 26.82477300818623 23.029426032048804 58-59 23.02047334153692 26.88118409380958 26.933027480192173 23.165315084461326 60-61 23.01485507147079 26.86510579248211 27.103722400819265 23.016316735227832 62-63 22.983749039641236 26.93403237402514 26.952303170988166 23.129915415345458 64-65 22.90660059946485 26.829249353442176 27.022234646364158 23.24191540072882 66-67 22.87969685093679 26.931109046511054 26.854554407235966 23.334639695316188 68-69 22.9165581838097 26.835370070424787 26.867069903155638 23.381001842609876 70-71 22.862476624799136 26.553405996292856 26.96198669337857 23.622130685529438 72-73 22.781034547336436 26.624844812418296 26.959657166765787 23.63446347347948 74-75 22.73416995312627 26.619957374230687 26.937275440486076 23.70859723215697 76-77 22.702881213327082 26.624844812418296 26.88036190794624 23.791912066308377 78-79 22.689680562521296 26.489503883914665 27.01935699584248 23.80145855772156 80-81 22.665654464514915 26.53997696052503 26.922567448930838 23.871801126029215 82-83 22.63505087960184 26.477764896865917 27.04365715580331 23.84352706772893 84-85 22.640121025759083 26.389060177610418 27.067089452908395 23.90372934372211 86-87 22.57493995759348 26.268381563669617 27.12815959175731 24.02851888697959 88-89 22.6227180916518 26.106136886637927 27.135422233550116 24.13572278816016 90-91 22.68529557125017 26.094260868611958 27.123683246501372 24.0967603136365 92-93 22.514646327615488 25.875924844903775 27.362528239800554 24.246900587680184 94-95 22.426763794223323 25.852720932760725 27.442645684483434 24.277869588532518 96-97 22.34559577871507 25.77301458100952 27.460733773476832 24.420655866798583 98-99 22.352493004568615 25.707605127881877 27.41998989624928 24.519911971300232 100-101 22.597595745827633 25.550841689939098 27.54062283319767 24.310939731035596 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 162.5 2 105.5 3 137.0 4 127.5 5 126.0 6 163.5 7 273.5 8 507.5 9 642.5 10 553.0 11 478.0 12 489.5 13 514.0 14 580.5 15 641.0 16 670.5 17 746.0 18 845.0 19 917.5 20 1000.0 21 1132.5 22 1259.5 23 1439.5 24 1677.0 25 1896.5 26 2258.0 27 2722.5 28 3153.5 29 3770.0 30 4715.5 31 5880.0 32 7665.5 33 10176.5 34 13019.0 35 16624.5 36 20897.5 37 25184.0 38 29750.5 39 34145.0 40 37685.0 41 40928.5 42 43792.5 43 46249.5 44 48482.5 45 50398.0 46 52150.0 47 53366.5 48 53376.5 49 52635.0 50 51224.5 51 48656.0 52 45398.0 53 42517.0 54 39022.5 55 34407.0 56 29855.0 57 25541.5 58 21992.0 59 18555.5 60 14909.0 61 11812.5 62 9160.5 63 6792.0 64 4882.5 65 3456.5 66 2531.5 67 2022.0 68 1534.5 69 1088.0 70 847.5 71 609.0 72 403.0 73 283.0 74 212.0 75 160.5 76 119.5 77 84.0 78 62.5 79 53.0 80 43.5 81 32.0 82 26.5 83 24.5 84 17.5 85 16.0 86 13.0 87 10.0 88 10.0 89 4.5 90 4.5 91 4.0 92 2.5 93 2.5 94 2.0 95 1.5 96 0.5 97 2.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2838496203785163E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1094643.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.752456296270374 #Duplication Level Percentage of deduplicated Percentage of total 1 69.37616343310421 41.45396173539469 2 16.990074776389264 20.303974010931302 3 6.434096884668144 11.53359278721308 4 2.9563546827065887 7.0659781589879875 5 1.5373636307731116 4.593062657962295 6 0.9057408817301764 3.247214547079665 7 0.5465773462187699 2.2861537294727943 8 0.34082125075662273 1.6291925512540242 9 0.24020241163318495 1.2917415693033567 >10 0.6625844776400238 5.622673178107554 >50 0.007402861936409755 0.2861636944241646 >100 0.002155476345565972 0.22382700397228797 >500 0.0 0.0 >1k 4.6188609788733653E-4 0.462464375896807 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2066 0.18873733262808057 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1884 0.17211090739172497 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.135398481514064E-5 0.0 9 0.0 0.0 0.0 1.8270796963028128E-4 9.135398481514064E-5 10-11 0.0 0.0 0.0 2.740619544454219E-4 9.135398481514064E-5 12-13 0.0 0.0 0.0 2.740619544454219E-4 9.135398481514064E-5 14-15 0.0 0.0 0.0 2.740619544454219E-4 9.135398481514064E-5 16-17 0.0 0.0 0.0 2.740619544454219E-4 9.135398481514064E-5 18-19 0.0 0.0 0.0 2.740619544454219E-4 9.135398481514064E-5 20-21 0.0 0.0 0.0 5.024469164832735E-4 9.135398481514064E-5 22-23 0.0 0.0 0.0 0.001141924810189258 1.3703097722271096E-4 24-25 0.0 0.0 0.0 0.0026492655596390786 1.8270796963028128E-4 26-27 0.0 0.0 0.0 0.004613376233164603 1.8270796963028128E-4 28-29 0.0 0.0 0.0 0.013429035767825675 1.8270796963028128E-4 30-31 0.0 0.0 0.0 0.02950733709529043 1.8270796963028128E-4 32-33 0.0 0.0 0.0 0.05462968291945411 2.740619544454219E-4 34-35 0.0 0.0 0.0 0.09514517518496898 2.740619544454219E-4 36-37 0.0 0.0 0.0 0.16251873898613522 2.740619544454219E-4 38-39 0.0 0.0 0.0 0.2497617944845945 2.740619544454219E-4 40-41 0.0 0.0 0.0 0.347510558236795 2.740619544454219E-4 42-43 0.0 0.0 0.0 0.43680907839359495 2.740619544454219E-4 44-45 0.0 0.0 0.0 0.5452919353615745 3.1973894685299225E-4 46-47 0.0 0.0 0.0 0.6884893065593074 3.6541593926056257E-4 48-49 0.0 0.0 0.0 0.8369852088763186 3.6541593926056257E-4 50-51 0.0 0.0 0.0 0.986623036003519 3.6541593926056257E-4 52-53 0.0 0.0 0.0 1.1279476505125414 5.481239088908439E-4 54-55 0.0 0.0 0.0 1.2965414294888835 7.308318785211251E-4 56-57 0.0 0.0 0.0 1.5017681563760972 7.308318785211251E-4 58-59 0.0 0.0 0.0 1.7221596447426237 7.308318785211251E-4 60-61 0.0 0.0 0.0 1.9499964828715846 8.221858633362658E-4 62-63 0.0 0.0 0.0 2.1721693739420065 8.221858633362658E-4 64-65 0.0 0.0 0.0 2.4223422613582692 8.221858633362658E-4 66-67 0.0 0.0 0.0 2.723947442225456 8.221858633362658E-4 68-69 0.0 0.0 0.0 3.0768478855663446 8.221858633362658E-4 70-71 0.0 0.0 0.0 3.4565607234504765 8.221858633362658E-4 72-73 0.0 0.0 0.0 3.839836366742399 8.221858633362658E-4 74-75 0.0 0.0 0.0 4.236860784749 8.221858633362658E-4 76-77 0.0 0.0 0.0 4.666863991273868 8.221858633362658E-4 78-79 0.0 0.0 0.0 5.139894924646666 8.221858633362658E-4 80-81 0.0 0.0 0.0 5.661663208918341 8.678628557438361E-4 82-83 0.0 0.0 0.0 6.215314033890501 9.135398481514064E-4 84-85 0.0 0.0 0.0 6.782302540645672 9.135398481514064E-4 86-87 0.0 0.0 0.0 7.408077336629385 9.592168405589767E-4 88-89 0.0 0.0 0.0 8.125525856375093 0.001004893832966547 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1720 0.0 52.470757 1 GTATCAA 2985 0.0 39.782112 1 TCAACGC 3255 0.0 35.168854 4 ATCAACG 3370 0.0 33.96873 3 CAACGCA 3380 0.0 33.587162 5 AACGCAG 3440 0.0 33.001343 6 TATCAAC 3520 0.0 32.65614 2 ACGCAGA 3795 0.0 29.663933 7 CGCAGAG 3845 0.0 28.784037 8 GCAGAGT 4490 0.0 23.802814 9 GTACATG 3080 0.0 23.74992 1 GAGTACT 2250 0.0 23.11659 12-13 TACATGG 3170 0.0 22.77595 2 CAGAGTA 4045 0.0 22.252705 10-11 ACATGGG 3320 0.0 21.031555 3 GTACTTT 2620 0.0 19.852034 14-15 AGAGTAC 3680 0.0 19.684036 10-11 TCGCCGG 75 7.7242835E-4 18.999937 94-95 GTCTACG 125 0.006031082 18.999935 1 CGACGGA 65 0.0075654048 18.269169 32-33 >>END_MODULE