FastQCFastQC Report
Fri 10 Feb 2017
SRR2031839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094643
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.22801954609859104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.21093635093815977No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT20580.18800650074955944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.11866882627486769No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.053.880051
GTATCAA29550.033.8018071
TCAACGC34300.028.6654475
CAACGCA35200.027.9325286
ATCAACG35950.027.3497894
AACGCAG36350.027.0488287
TATCAAC37100.026.7580783
GTACATG26300.024.2341141
ACGCAGA41150.023.893688
CGCAGAG41700.023.0090078
TATACCG1050.002196480322.6184715
GTAGCCC5500.021.590363
GAGTACT28100.021.38290612-13
TACATGG27600.021.167942
GTACTAA2551.1734301E-820.5177921
ACATGGG28600.020.2617233
CAGAGTA44450.020.08947610-11
TAGGCCG1200.00476405919.7911625
GCAGAGT47000.019.7069459
AGAGTAC40700.019.0812110-11
Produced by FastQC (version 0.11.5)