FastQCFastQC Report
Fri 10 Feb 2017
SRR2031837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15300
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2131.392156862745098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1921.2549019607843137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.5359477124183006No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.24183006535947713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.16993464052287582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.1503267973856209No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.13725490196078433No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT170.1111111111111111No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.10457516339869283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG160.10457516339869283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2150.048.604651
CATCTCA205.305879E-447.50000490-91
AACGCAG2250.046.4444476
CAACGCA2400.045.5208365
ACGCAGA2400.043.5416687
CGCAGAG2450.042.6530658
TCAACGC2600.042.0192344
TATCAAC2550.040.9803922
ATCAACG2550.040.9803923
GCAGAGT2500.039.8999989
GGTATCA1101.4078978E-938.8636361
AGTACTT1550.029.11290412-13
AGAGTAC2150.028.7209310-11
CAGAGTA2150.026.51162710-11
GAGTACT1500.025.33333212-13
TACTTTT1800.025.06944514-15
GTACTTT1750.023.07142614-15
GGTCGCC550.00281454121.5909192-93
ACTTTTT1900.021.2516-17
GGAAGCA704.70498E-420.35714120-21
Produced by FastQC (version 0.11.5)