FastQCFastQC Report
Fri 10 Feb 2017
SRR2031837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15300
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT10176.647058823529411No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG6524.261437908496732TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT4112.686274509803922RNA PCR Primer, Index 3 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT3822.496732026143791TruSeq Adapter, Index 3 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2701.7647058823529411No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA2351.5359477124183005RNA PCR Primer, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2311.5098039215686274No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT2181.4248366013071896TruSeq Adapter, Index 3 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.823529411764706No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1100.7189542483660131TruSeq Adapter, Index 3 (95% over 24bp)
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC530.3464052287581699Illumina PCR Primer Index 12 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.2156862745098039No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.20915032679738566No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT300.19607843137254902No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.18954248366013074No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC260.16993464052287582No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT260.16993464052287582TruSeq Adapter, Index 3 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.16339869281045752No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC210.13725490196078433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA190.12418300653594772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG190.12418300653594772No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1176470588235294No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT180.1176470588235294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA170.1111111111111111No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.10457516339869283No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10457516339869283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2200.038.8636361
AACGCAG2600.032.8846176
TCAACGC2600.032.8846174
TATCAAC2600.032.8846172
ATCAACG2600.032.8846173
CAACGCA2600.032.8846175
GATACCA600.003831378931.6666686
CTTATAC3600.031.6666661
GGTATCA1351.0424628E-831.6666661
TTATACA3650.031.2328762
TATACAC3700.030.8108123
ACGCAGA2800.030.5357117
GCAGAGT2550.029.803929
CGCAGAG2550.029.803928
TACACAT4000.029.6875025
ATACACA3950.028.8607584
ACACATC4250.027.9411776
CACATCT4350.027.2988537
ACCACTG700.008139837527.1428559
ATACCAC700.008139837527.1428557
Produced by FastQC (version 0.11.5)