FastQCFastQC Report
Fri 10 Feb 2017
SRR2031835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080396
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.18114554102784186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44230.14358543511938077No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52700.045.9677431
GTATCAA85300.038.47891
TCAACGC104800.030.6393134
CAACGCA105500.030.4810415
TATCAAC108050.030.2012942
AACGCAG107400.029.9860346
ATCAACG107650.029.9605223
GTACATG114700.026.835221
ACGCAGA122750.026.0040747
TACATGG115400.025.7668972
CGCAGAG126100.025.0872338
ACATGGG120300.023.9671653
CATGGGG85050.022.3398024
GTATAGA8750.022.2571431
GCAGAGT139300.021.9597999
AGAGTAC111000.021.24662210-11
GAGTACT58450.020.51967412-13
CAGAGTA129600.019.6084110-11
GTACTTT60050.019.53788614-15
AGTACTT58800.018.82227712-13
Produced by FastQC (version 0.11.5)