FastQCFastQC Report
Fri 10 Feb 2017
SRR2031835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080396
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73200.23763178500426566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57930.18806023641116273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38790.12592536803709653No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT32630.10592793913509822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.044.4967231
GTATCAA93050.037.4799421
TCAACGC112050.030.6062414
CAACGCA112800.030.3185215
TATCAAC115900.029.7534832
ATCAACG115450.029.7460293
AACGCAG116600.029.4119136
ACGCAGA130250.026.0743187
GTACATG107900.026.0601771
TACATGG108200.025.1981852
CGCAGAG134350.025.137188
ACATGGG113850.023.78083
GTATAAT7950.022.7419571
GCAGAGT150300.021.8691439
AGAGTAC119600.021.52547510-11
GAGTACT71500.021.39116712-13
TATAGCG1353.798473E-421.1106742
CATGGGG79800.020.8329014
GTACTTT76400.019.80161914-15
CAGAGTA139200.019.74007210-11
Produced by FastQC (version 0.11.5)