FastQCFastQC Report
Fri 10 Feb 2017
SRR2031831_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031831_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547478
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52150.3369999444257043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43290.2797454955740889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.15780515134948606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23000.054.3152161
GTATCAA42700.041.8266941
TCAACGC49650.035.2064484
ATCAACG50550.034.4856573
CAACGCA50900.034.435175
AACGCAG53150.033.1561626
TATCAAC53600.032.6119422
ACGCAGA59650.029.6227997
CGCAGAG60550.029.0255998
ACGACGT700.00839245227.1428598
GCAGAGT68000.025.1470589
GTACATG37150.024.2934041
TACATGG37150.022.3755042
AGAGTAC60150.022.11138710-11
CAGAGTA64350.022.03379810-11
GAGTACT45900.021.88725712-13
ACATGGG37750.021.0132473
TACACCG1908.254285E-620.05
AGTACTT50000.019.332512-13
CGCCGTA2250.019.094-95
Produced by FastQC (version 0.11.5)