##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031831_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1547478 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.516009920658 34.0 31.0 34.0 31.0 34.0 2 32.64985156493339 34.0 31.0 34.0 31.0 34.0 3 32.72723037096489 34.0 31.0 34.0 31.0 34.0 4 35.92462703831654 37.0 37.0 37.0 35.0 37.0 5 35.947227036507144 37.0 37.0 37.0 35.0 37.0 6 36.02160030708029 37.0 37.0 37.0 35.0 37.0 7 36.0408296596139 37.0 37.0 37.0 35.0 37.0 8 36.03713849243737 37.0 37.0 37.0 35.0 37.0 9 37.86025843339937 39.0 39.0 39.0 35.0 39.0 10-11 37.89977240387262 39.0 39.0 39.0 36.0 39.0 12-13 37.8722634505951 39.0 39.0 39.0 36.0 39.0 14-15 39.43507726765744 41.0 40.0 41.0 37.0 41.0 16-17 39.37248768641622 41.0 40.0 41.0 37.0 41.0 18-19 39.38128264182108 41.0 40.0 41.0 37.0 41.0 20-21 39.32369539340786 41.0 40.0 41.0 37.0 41.0 22-23 39.23725409989673 41.0 40.0 41.0 36.0 41.0 24-25 39.19939992684872 41.0 39.5 41.0 36.0 41.0 26-27 39.07959046913753 41.0 39.0 41.0 36.0 41.0 28-29 38.962299625584336 41.0 39.0 41.0 35.0 41.0 30-31 38.804925821239465 41.0 39.0 41.0 35.0 41.0 32-33 38.67343897619223 40.5 38.5 41.0 35.0 41.0 34-35 38.483191360394144 40.0 38.0 41.0 35.0 41.0 36-37 38.367039789903316 40.0 38.0 41.0 34.0 41.0 38-39 38.17655210607194 40.0 38.0 41.0 34.0 41.0 40-41 38.08501639441724 40.0 38.0 41.0 33.5 41.0 42-43 37.91990257696717 40.0 38.0 41.0 33.0 41.0 44-45 38.07452254571632 40.0 38.0 41.0 33.0 41.0 46-47 37.950128208607815 40.0 38.0 41.0 33.0 41.0 48-49 37.777896680922126 40.0 38.0 41.0 33.0 41.0 50-51 36.72033172684846 39.0 36.5 40.0 31.5 40.5 52-53 36.74114042332104 39.5 37.0 40.0 31.0 40.5 54-55 37.52998976399018 40.0 38.0 41.0 32.0 41.0 56-57 37.5308350102554 40.0 37.0 41.0 32.0 41.0 58-59 37.40778253390355 40.0 37.0 41.0 32.0 41.0 60-61 37.215329070914095 40.0 36.5 41.0 32.0 41.0 62-63 36.94987909359617 39.5 36.0 41.0 31.0 41.0 64-65 36.63274534436031 39.0 35.0 41.0 31.0 41.0 66-67 36.36657160877247 39.0 35.0 41.0 31.0 41.0 68-69 36.04411791314642 38.5 35.0 41.0 30.5 41.0 70-71 35.68696614749935 37.0 35.0 40.0 30.0 41.0 72-73 35.28720149817961 37.0 35.0 39.0 29.0 41.0 74-75 34.933595178735985 36.0 35.0 39.0 29.0 41.0 76-77 34.556327133568296 36.0 35.0 39.0 29.0 40.5 78-79 34.183641059840596 35.0 35.0 37.0 29.0 39.0 80-81 33.83494272616477 35.0 34.0 37.0 29.0 39.0 82-83 33.547831051556145 35.0 34.0 36.5 28.5 39.0 84-85 33.245376024731854 35.0 34.0 36.0 27.5 37.0 86-87 32.99284804048911 35.0 34.0 36.0 27.0 37.0 88-89 32.793217415691856 35.0 34.0 35.0 26.5 36.5 90-91 32.62121206246551 35.0 34.0 35.0 26.0 36.0 92-93 32.495830312288774 35.0 34.0 35.0 26.0 36.0 94-95 32.33686940945202 35.0 34.0 35.0 25.0 36.0 96-97 32.236174924619284 35.0 34.0 35.0 25.0 36.0 98-99 32.12981896996274 35.0 34.0 35.0 25.0 35.0 100-101 30.603785643479263 34.0 30.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.282168216104985 1101 2 0.24398658105324955 1101 3 0.33041881440479415 1101 4 0.2968099954695731 1101 5 0.2642668284950602 1101 6 0.30663124295839594 1101 7 0.2878311712392545 1101 8 0.3933372949903813 1101 9 0.4251093322470396 1101 10-11 0.42675413633984505 1101 12-13 0.4126197150039488 1101 14-15 0.4586843003931591 1101 16-17 0.5236216773689009 1101 18-19 0.4975631045482416 1101 20-21 0.4850142795215717 1101 22-23 0.4410167812091146 1101 24-25 0.4873350260669511 1101 26-27 0.4198910427047693 1101 28-29 0.498112558238617 1101 30-31 0.4584216575776523 1101 32-33 0.2258048199489835 1101 34-35 0.40408998198709867 1101 36-37 0.46698595066588666 1101 38-39 0.5016678492338187 1101 40-41 0.45039494548576187 1101 42-43 0.34139537184370283 1101 44-45 0.5199562904327735 1101 46-47 0.5457175520630742 1101 48-49 0.4416382983330607 1101 50-51 0.47514831634572374 1101 52-53 0.49488553286615655 1101 54-55 0.42764548064869956 1101 56-57 0.46042903086894427 1101 58-59 0.47092455947460365 1101 60-61 0.4104272376430771 1101 62-63 0.46811936842551916 1101 64-65 0.41316380888716964 1101 66-67 0.34557334348015445 1101 68-69 0.5008597276029931 1101 70-71 0.4961328083994232 1101 72-73 0.44189570726640426 1101 74-75 0.4649310481109836 1101 76-77 0.3535104802450064 1101 78-79 0.30735706714988 1101 80-81 0.47295238724903754 1101 82-83 0.40557658433930754 1101 84-85 0.3644406210882991 1101 86-87 0.369319836741532 1101 88-89 0.47897785341530863 1101 90-91 0.49114014022936203 1101 92-93 0.40108365319799333 1101 94-95 0.27557227879562873 1101 96-97 0.09039532956533947 1101 98-99 0.2446544055650577 1101 100-101 0.12742369545898313 1102 1 0.17304381146789183 1102 2 0.21022388746668952 1102 3 0.12866342992766988 1102 4 0.15806871997006766 1102 5 0.1068304087604588 1102 6 0.16501125673786987 1102 7 0.140588562725938 1102 8 0.23157831586927102 1102 9 0.24300167265982253 1102 10-11 0.28912869824259957 1102 12-13 0.3408870966053641 1102 14-15 0.3786914896811169 1102 16-17 0.3184858297817996 1102 18-19 0.33482009163747506 1102 20-21 0.3440907555722603 1102 22-23 0.3420367614385782 1102 24-25 0.34020624794634813 1102 26-27 0.4566582595516664 1102 28-29 0.35259537883592174 1102 30-31 0.3076188537553435 1102 32-33 0.36031639769813495 1102 34-35 0.5145414093603122 1102 36-37 0.40707222212141403 1102 38-39 0.2742676754926947 1102 40-41 0.20639949870146523 1102 42-43 0.2913219811957646 1102 44-45 0.3606341803767634 1102 46-47 0.2683892712015634 1102 48-49 0.19283501522489388 1102 50-51 0.32689621198122154 1102 52-53 0.4233056868367484 1102 54-55 0.5485573220050739 1102 56-57 0.30800863725542627 1102 58-59 0.36173275526287796 1102 60-61 0.45008814289425914 1102 62-63 0.5147883316702888 1102 64-65 0.44524301087620444 1102 66-67 0.4827838997513112 1102 68-69 0.32990445096518783 1102 70-71 0.27814910411879623 1102 72-73 0.4119628072873738 1102 74-75 0.33161618725365827 1102 76-77 0.14412696168766104 1102 78-79 0.233705807526718 1102 80-81 0.14926068788109603 1102 82-83 0.11740595857169467 1102 84-85 0.15394575843365743 1102 86-87 0.2805590902538029 1102 88-89 0.2635298709092453 1102 90-91 0.26453528146890903 1102 92-93 0.23054625392291683 1102 94-95 0.4062061343508532 1102 96-97 0.30795965871490694 1102 98-99 0.22070059674020825 1102 100-101 0.3503754972742783 1103 1 0.21483579141776943 1103 2 0.2638579726797232 1103 3 0.3212490606711924 1103 4 0.2851781602374004 1103 5 0.22318379221911044 1103 6 0.21766372123243372 1103 7 0.22757686681950418 1103 8 0.22524999506726573 1103 9 0.23471011042089174 1103 10-11 0.23176654285413179 1103 12-13 0.24411391364797197 1103 14-15 0.2603644220119463 1103 16-17 0.2705430561811326 1103 18-19 0.353282915363458 1103 20-21 0.3744061273349999 1103 22-23 0.3773646645296367 1103 24-25 0.29297358295052334 1103 26-27 0.31359226122251727 1103 28-29 0.30124826104144375 1103 30-31 0.3069191879241018 1103 32-33 0.2432633484082416 1103 34-35 0.32286429992004884 1103 36-37 0.34548074342467316 1103 38-39 0.4146603237800761 1103 40-41 0.3536739347916935 1103 42-43 0.43181905721915825 1103 44-45 0.45367929315871436 1103 46-47 0.5109644591714826 1103 48-49 0.3727431188172048 1103 50-51 0.18630515099542322 1103 52-53 0.2145859708801865 1103 54-55 0.2642423679532797 1103 56-57 0.2450804835378051 1103 58-59 0.24026241273990223 1103 60-61 0.2850923200036988 1103 62-63 0.28893202423503084 1103 64-65 0.4149589674342735 1103 66-67 0.42753118463017614 1103 68-69 0.32736685698022683 1103 70-71 0.24815745833767977 1103 72-73 0.25267918155637403 1103 74-75 0.2051124278551697 1103 76-77 0.14376146461164296 1103 78-79 0.20871625030031993 1103 80-81 0.24072894184935478 1103 82-83 0.30222216575632643 1103 84-85 0.36717675258570637 1103 86-87 0.4800473943473733 1103 88-89 0.43915643732528764 1103 90-91 0.4738502355207146 1103 92-93 0.2945917844158146 1103 94-95 0.29479218280532393 1103 96-97 0.2971618308118167 1103 98-99 0.30661329515290703 1103 100-101 0.2918333084689344 1104 1 0.3092822950006635 1104 2 0.2632896504657367 1104 3 0.26426652216530755 1104 4 0.325854381306506 1104 5 0.18802812135179892 1104 6 0.2207297783941229 1104 7 0.2497805567956206 1104 8 0.3234029478718128 1104 9 0.3531043942525329 1104 10-11 0.32816819840144973 1104 12-13 0.2826270097685537 1104 14-15 0.37329066367243513 1104 16-17 0.41332062804506364 1104 18-19 0.49440391033915887 1104 20-21 0.4734352435527782 1104 22-23 0.4617100644143264 1104 24-25 0.4005048818550634 1104 26-27 0.4267789838977407 1104 28-29 0.34516659561025875 1104 30-31 0.3441617956170404 1104 32-33 0.44219980339547504 1104 34-35 0.4778793316307812 1104 36-37 0.3982482255101729 1104 38-39 0.4711939701024548 1104 40-41 0.4572043276753064 1104 42-43 0.43483569505788466 1104 44-45 0.4291981960497466 1104 46-47 0.4679974465695551 1104 48-49 0.4843154769425624 1104 50-51 0.39797531819746723 1104 52-53 0.5185879064703371 1104 54-55 0.45263706361262024 1104 56-57 0.4122022198033477 1104 58-59 0.27571665868340034 1104 60-61 0.34250315930853503 1104 62-63 0.4238611898721274 1104 64-65 0.5170819391828942 1104 66-67 0.43675509534593715 1104 68-69 0.2919054040521303 1104 70-71 0.2803438515507608 1104 72-73 0.29817481618284347 1104 74-75 0.4845231024289234 1104 76-77 0.463752610316277 1104 78-79 0.332636232179901 1104 80-81 0.46624681517115363 1104 82-83 0.3903038723702821 1104 84-85 0.4197166998717634 1104 86-87 0.35100015771117654 1104 88-89 0.4203153204485872 1104 90-91 0.4375867481638238 1104 92-93 0.36207489948046856 1104 94-95 0.3222868290453391 1104 96-97 0.2880666162495942 1104 98-99 0.23283127554990557 1104 100-101 0.2209145208896537 1105 1 0.13346899126521095 1105 2 0.0732234379733967 1105 3 0.1902501412712425 1105 4 0.08308608675659457 1105 5 0.0826660576653353 1105 6 0.16855203803894625 1105 7 0.10873072519733284 1105 8 0.20606139857603978 1105 9 0.2926175602047749 1105 10-11 0.2139545195894499 1105 12-13 0.21719624583713681 1105 14-15 0.27035798608517325 1105 16-17 0.29057587146208874 1105 18-19 0.2705951611046231 1105 20-21 0.2499106722116835 1105 22-23 0.30504193061150886 1105 24-25 0.21647146942395779 1105 26-27 0.2755150142100504 1105 28-29 0.27558798262801787 1105 30-31 0.29113726254906425 1105 32-33 0.2442684653673055 1105 34-35 0.32133080131847436 1105 36-37 0.3987142007091933 1105 38-39 0.4405132827172835 1105 40-41 0.425023334105191 1105 42-43 0.28478594000583257 1105 44-45 0.3480228286278191 1105 46-47 0.5072101685536339 1105 48-49 0.5791734836786375 1105 50-51 0.39224487576024103 1105 52-53 0.5018587270459633 1105 54-55 0.6297593079481985 1105 56-57 0.6826658986153475 1105 58-59 0.5518725068156769 1105 60-61 0.5599202980103115 1105 62-63 0.6263632948935651 1105 64-65 0.6802641574927506 1105 66-67 0.4677457949785122 1105 68-69 0.49638144508090676 1105 70-71 0.5232317495911758 1105 72-73 0.5761897809715819 1105 74-75 0.48298356629910444 1105 76-77 0.4078748163787367 1105 78-79 0.4488200196875525 1105 80-81 0.39186396918216815 1105 82-83 0.43343958884302936 1105 84-85 0.46514784653434305 1105 86-87 0.5391546280112394 1105 88-89 0.44900102161208366 1105 90-91 0.4184913332832281 1105 92-93 0.43226301185034544 1105 94-95 0.40965957093143857 1105 96-97 0.39000802010752267 1105 98-99 0.4047512464623395 1105 100-101 0.3802101041719581 1106 1 0.14024911285695651 1106 2 0.055430972977077886 1106 3 0.17409225623071478 1106 4 0.11284889073596105 1106 5 0.14764855582303227 1106 6 0.19049105793578036 1106 7 0.17413004354663286 1106 8 0.169557161287905 1106 9 0.24545101635805366 1106 10-11 0.18755229961892184 1106 12-13 0.1637251238769366 1106 14-15 0.24071009551774125 1106 16-17 0.35661377676791517 1106 18-19 0.2793836191002015 1106 20-21 0.3574958785491944 1106 22-23 0.2719857865437092 1106 24-25 0.29214966581304935 1106 26-27 0.3204839254112315 1106 28-29 0.34416158248063056 1106 30-31 0.3633943093434695 1106 32-33 0.36775363485883616 1106 34-35 0.38773371215118857 1106 36-37 0.43671922097448146 1106 38-39 0.34865279082339384 1106 40-41 0.36998303381042064 1106 42-43 0.1659696172350138 1106 44-45 0.2585990076993028 1106 46-47 0.22143502116970382 1106 48-49 0.2566453886160005 1106 50-51 0.20038162041756635 1106 52-53 0.30090349491626256 1106 54-55 0.31678721878091665 1106 56-57 0.34281811858586764 1106 58-59 0.33221701676409054 1106 60-61 0.34911452240234553 1106 62-63 0.3237382488360794 1106 64-65 0.46870165749275117 1106 66-67 0.31651398768300254 1106 68-69 0.42981779364392025 1106 70-71 0.36919918695300424 1106 72-73 0.35571838266650246 1106 74-75 0.37697237433152964 1106 76-77 0.3604427511613437 1106 78-79 0.31901484282683157 1106 80-81 0.3133845568756186 1106 82-83 0.24563330660279803 1106 84-85 0.33245601713125694 1106 86-87 0.3441094916811025 1106 88-89 0.3233387150535023 1106 90-91 0.4105337522220651 1106 92-93 0.43304529814363235 1106 94-95 0.4341798822799987 1106 96-97 0.49814061959168043 1106 98-99 0.44428030873205415 1106 100-101 0.6344356476502178 1107 1 0.2627486182318819 1107 2 0.27402931543349496 1107 3 0.204452123451361 1107 4 0.3273596515792434 1107 5 0.2879066401950823 1107 6 0.2165857181833104 1107 7 0.3055486282594231 1107 8 0.17578416122195506 1107 9 0.27684893673440314 1107 10-11 0.21762308596287738 1107 12-13 0.2232720473089742 1107 14-15 0.23498299665868672 1107 16-17 0.24332419027447827 1107 18-19 0.2423652190474428 1107 20-21 0.304855020319728 1107 22-23 0.24277498832876176 1107 24-25 0.3814679625441286 1107 26-27 0.3138937607560379 1107 28-29 0.390746690968399 1107 30-31 0.26174687809635344 1107 32-33 0.33589054707936583 1107 34-35 0.4269513252004984 1107 36-37 0.30111764696758314 1107 38-39 0.46740567613633033 1107 40-41 0.2954489706303107 1107 42-43 0.2678630420190302 1107 44-45 0.3663951782020618 1107 46-47 0.37074584356631846 1107 48-49 0.34624529188931064 1107 50-51 0.2919679379888507 1107 52-53 0.2883637600353026 1107 54-55 0.29461702378871735 1107 56-57 0.31587314287745727 1107 58-59 0.18396733002663979 1107 60-61 0.3861856824630223 1107 62-63 0.25530060457043646 1107 64-65 0.29724899860818255 1107 66-67 0.41496064511667186 1107 68-69 0.33864322396772906 1107 70-71 0.33368092979193165 1107 72-73 0.3868962498431827 1107 74-75 0.4162616530582568 1107 76-77 0.2618824398772972 1107 78-79 0.34227343281562383 1107 80-81 0.5178909210515741 1107 82-83 0.42284757818493546 1107 84-85 0.4017169373537186 1107 86-87 0.5326889284680192 1107 88-89 0.4970699467798525 1107 90-91 0.5608129405972804 1107 92-93 0.3869260165590802 1107 94-95 0.3552328841046162 1107 96-97 0.4660328572755077 1107 98-99 0.6193806626637652 1107 100-101 0.5576392792974296 1108 1 0.12527261468249407 1108 2 0.11553311758557783 1108 3 0.09118723786824035 1108 4 0.14644996999975035 1108 5 0.17768806458669673 1108 6 0.16180662481693986 1108 7 0.14989800183223423 1108 8 0.13959345795696265 1108 9 0.12640522259594178 1108 10-11 0.10188809198913162 1108 12-13 0.17222689588245288 1108 14-15 0.18158121557513596 1108 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15 2165.0 16 2532.0 17 3276.0 18 4206.0 19 5650.0 20 7439.0 21 9686.0 22 11356.0 23 11618.0 24 10623.0 25 9516.0 26 9488.0 27 9799.0 28 10995.0 29 12695.0 30 14930.0 31 18450.0 32 23892.0 33 31831.0 34 46881.0 35 76412.0 36 146704.0 37 333957.0 38 535644.0 39 187459.0 40 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.887265602483524 19.726290131426747 13.78830587575397 23.59813839033576 2 15.167065379927857 21.54188944850912 40.03003596820116 23.26100920336186 3 17.36438256311237 26.21917726778668 31.93143941303204 24.485000756068906 4 12.283793372183643 18.20045260740379 39.461433377405044 30.054320643007525 5 12.887420693541362 38.253532521948614 35.79999198696201 13.059054797548011 6 29.550662432680785 38.74394337108508 18.436191015316535 13.269203180917597 7 25.41851968170145 34.030079910667546 23.42941224366356 17.121988163967437 8 24.848236937778758 35.061047717641216 21.66815941809835 18.422555926481667 9 26.00754259511282 15.483451137916015 21.210382312381824 37.29862395458934 10-11 23.4067624870919 26.37058491300038 30.41849383319181 19.804158766715908 12-13 24.566908220989248 24.84477969961447 29.447300704759616 21.14101137463667 14-15 22.051880543697553 26.21197199572466 27.214441820820717 24.521705639757077 16-17 21.28036715223092 29.080833459344817 27.804692538439962 21.8341068499843 18-19 21.223145014016353 28.181951536629278 29.80268540166645 20.792218047687918 20-21 22.178344377109077 27.54733831434114 29.645074114139263 20.629243194410517 22-23 21.817229534664985 27.502000838138734 29.276405812947726 21.404363814248555 24-25 21.7296633967151 27.456769077616467 29.47108336841738 21.342484157251057 26-27 21.66124494176977 27.676193134894323 29.37356783101278 21.288994092323122 28-29 21.68266689413355 27.680942798540592 29.385910494365667 21.250479812960187 30-31 21.885351520344713 27.51525385175104 29.374084801205573 21.22530982669867 32-33 21.68441166853422 27.699489104206975 29.258154235472166 21.35794499178664 34-35 21.92981095692475 27.7678584122036 29.101738441515806 21.200592189355845 36-37 21.91604662554169 27.63399544290775 29.13295051690557 21.31700741464499 38-39 21.76176333363059 27.816712095422357 29.10529261159125 21.316231959355804 40-41 21.842733790076498 27.74055592389682 29.07702080417298 21.3396894818537 42-43 21.767740801484738 27.73457845604267 29.009039223820952 21.488641518651637 44-45 21.917629846757112 27.93299807816331 28.78903609615129 21.360335978928294 46-47 21.973204142482157 27.88876481604262 28.507287341080133 21.630743700395094 48-49 21.915141927704305 27.957554162320886 28.483506712211742 21.64379719776307 50-51 21.910327642783937 28.128994402505235 28.303601085120434 21.65707686959039 52-53 21.884705307603728 28.2443756874088 28.203631974089454 21.667287030898017 54-55 21.80428413198766 28.183534757844697 28.306864459462428 21.70531665070521 56-57 21.93918104166909 28.205635233586516 28.185473396067668 21.669710328676725 58-59 21.886514703278497 28.100334867442378 28.27439226922774 21.738758160051386 60-61 21.8978880475199 28.073484728054293 28.31594374847332 21.712683475952485 62-63 21.739889032348117 28.087442923259655 28.383699154366006 21.788968890026222 64-65 21.75352412118298 27.96495329820521 28.450129824139665 21.83139275647214 66-67 21.698337553102533 28.10980188409787 28.28324538377929 21.908615179020316 68-69 21.738079636673348 28.111546658498536 28.246960538372758 21.903413166455355 70-71 21.708030744217368 27.906083317501125 28.31494211872479 22.070943819556724 72-73 21.602181097243385 27.86055762989845 28.374490622806913 22.162770650051243 74-75 21.57701111098187 28.041012537819597 28.297849791725632 22.084126559472896 76-77 21.624701611266847 27.935453686579066 28.22660483703161 22.21323986512248 78-79 21.55772166066335 27.883918220485203 28.256427555028246 22.3019325638232 80-81 21.573101523898885 27.923918789152413 28.164794588356024 22.338185098592678 82-83 21.57804505136745 27.930962508029193 28.18292085574076 22.308071584862596 84-85 21.56124352010174 27.825468278062758 28.19981931891762 22.413468882917883 86-87 21.426702027427854 27.806889661759328 28.22828499015818 22.53812332065464 88-89 21.425958882775717 27.625303881541452 28.318237803703834 22.630499431979 90-91 21.44618534156867 27.664432063008327 28.36476512105503 22.52461747436797 92-93 21.427218997620646 27.46362145374603 28.427964727123744 22.681194821509578 94-95 21.386539905575393 27.33066318228757 28.527643042421282 22.75515386971576 96-97 21.27632832259974 27.324911888892768 28.57546278525446 22.823297003253035 98-99 21.26999543773805 27.333086480066278 28.428514007953588 22.968404074242088 100-101 21.471290706556086 27.203650068046205 28.454524070778387 22.870535154619322 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 335.0 1 214.5 2 161.0 3 249.0 4 223.0 5 215.5 6 334.5 7 656.0 8 1209.0 9 1568.5 10 1434.0 11 1308.0 12 1354.5 13 1405.0 14 1512.0 15 1649.0 16 1788.5 17 1936.0 18 2166.0 19 2419.5 20 2751.5 21 3266.0 22 4018.0 23 4977.5 24 6126.0 25 7525.0 26 9336.0 27 11771.0 28 14719.0 29 18035.0 30 21406.0 31 25006.0 32 28912.5 33 32126.5 34 35091.0 35 38287.0 36 41849.5 37 45572.0 38 48305.0 39 50263.0 40 52237.0 41 54324.5 42 56160.0 43 58514.0 44 60320.5 45 61168.5 46 61969.5 47 62388.0 48 62241.0 49 60837.0 50 58172.5 51 55049.5 52 51842.5 53 48207.0 54 43880.0 55 39496.0 56 34952.5 57 29956.0 58 25892.0 59 22339.5 60 18341.5 61 14575.5 62 11336.5 63 8491.5 64 6026.5 65 4234.5 66 3182.0 67 2434.0 68 1825.5 69 1349.0 70 1035.5 71 813.0 72 626.5 73 487.0 74 370.0 75 279.0 76 199.0 77 150.0 78 117.0 79 76.5 80 54.5 81 43.5 82 28.5 83 23.0 84 25.0 85 20.0 86 16.0 87 15.5 88 11.0 89 6.5 90 4.0 91 4.5 92 3.5 93 2.5 94 2.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.693191114833297E-5 24-25 2.2617445934611024E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1547478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.18506240772075 #Duplication Level Percentage of deduplicated Percentage of total 1 73.07620993560772 46.17324885301077 2 15.779475715218899 19.940543156544404 3 5.555840634796558 10.531384117109143 4 2.3659607119967436 5.979735009669186 5 1.1985057527482503 3.7863830391705258 6 0.6488373293978169 2.459809629027596 7 0.40254844590683936 1.7804534073728249 8 0.24428558350004345 1.234815987100538 9 0.1700891481536823 0.967238409686983 >10 0.5412368840355867 5.326013313408658 >50 0.012588216245264986 0.5205623617259698 >100 0.004010365030960753 0.48818667625889106 >500 1.0281934042260587E-4 0.03293799745750779 >1k 2.0563868084521168E-4 0.4398879305419827 >5k 1.0281934042260584E-4 0.3388001119149963 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5215 0.3369999444257043 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4329 0.2797454955740889 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2442 0.15780515134948606 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.462127409888865E-5 0.0 10-11 0.0 0.0 0.0 6.462127409888865E-5 0.0 12-13 0.0 0.0 0.0 9.693191114833297E-5 0.0 14-15 0.0 0.0 0.0 1.292425481977773E-4 0.0 16-17 0.0 0.0 0.0 1.9386382229666594E-4 0.0 18-19 0.0 0.0 0.0 1.9386382229666594E-4 0.0 20-21 0.0 0.0 0.0 2.907957334449989E-4 0.0 22-23 0.0 0.0 0.0 6.785233780383308E-4 0.0 24-25 0.0 0.0 0.0 0.0021001914082138806 0.0 26-27 0.0 0.0 0.0 0.0037157232606860966 0.0 28-29 0.0 0.0 0.0 0.008853114551547744 0.0 30-31 0.0 0.0 0.0 0.020904982170990474 0.0 32-33 0.0 0.0 0.0 0.038320415540640966 0.0 34-35 0.0 0.0 0.0 0.06908014201171196 0.0 36-37 0.0 0.0 0.0 0.11748147631177955 0.0 38-39 0.0 0.0 0.0 0.18497839710806874 0.0 40-41 0.0 0.0 0.0 0.25948672614408735 0.0 42-43 0.0 0.0 0.0 0.32769448095546433 0.0 44-45 0.0 0.0 0.0 0.4147070265296179 0.0 46-47 0.0 0.0 0.0 0.5276973242915247 0.0 48-49 0.0 0.0 0.0 0.6490237664121881 0.0 50-51 0.0 0.0 0.0 0.7680884639393905 0.0 52-53 0.0 0.0 0.0 0.8850852806954284 0.0 54-55 0.0 0.0 0.0 1.0139724118856617 0.0 56-57 0.0 0.0 0.0 1.178562797015531 0.0 58-59 0.0 0.0 0.0 1.3679677513993735 0.0 60-61 0.0 0.0 0.0 1.563027067266869 0.0 62-63 0.0 0.0 0.0 1.754790698155321 0.0 64-65 0.0 0.0 0.0 1.9518209628828327 0.0 66-67 0.0 0.0 0.0 2.193569149286775 0.0 68-69 0.0 0.0 0.0 2.4864327635029384 0.0 70-71 0.0 0.0 0.0 2.7960332877107135 0.0 72-73 0.0 0.0 0.0 3.116910224248745 0.0 74-75 0.0 0.0 0.0 3.4499036496803184 0.0 76-77 0.0 0.0 0.0 3.8126551718344297 0.0 78-79 0.0 0.0 0.0 4.223452611281065 0.0 80-81 0.0 0.0 0.0 4.673345921557528 0.0 82-83 0.0 0.0 0.0 5.1403961801072455 0.0 84-85 0.0 0.0 0.0 5.62744672299057 0.0 86-87 0.0 0.0 0.0 6.153205409059128 0.0 88-89 0.0 0.0 0.0 6.773795814867804 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2300 0.0 54.315216 1 GTATCAA 4270 0.0 41.826694 1 TCAACGC 4965 0.0 35.206448 4 ATCAACG 5055 0.0 34.485657 3 CAACGCA 5090 0.0 34.43517 5 AACGCAG 5315 0.0 33.156162 6 TATCAAC 5360 0.0 32.611942 2 ACGCAGA 5965 0.0 29.622799 7 CGCAGAG 6055 0.0 29.025599 8 ACGACGT 70 0.008392452 27.142859 8 GCAGAGT 6800 0.0 25.147058 9 GTACATG 3715 0.0 24.293404 1 TACATGG 3715 0.0 22.375504 2 AGAGTAC 6015 0.0 22.111387 10-11 CAGAGTA 6435 0.0 22.033798 10-11 GAGTACT 4590 0.0 21.887257 12-13 ACATGGG 3775 0.0 21.013247 3 TACACCG 190 8.254285E-6 20.0 5 AGTACTT 5000 0.0 19.3325 12-13 CGCCGTA 225 0.0 19.0 94-95 >>END_MODULE