FastQCFastQC Report
Fri 10 Feb 2017
SRR2031831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547478
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58890.3805546831683552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50670.32743599585906874No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT29390.1899219245766337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.1802287334618004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.059.6934781
GTATCAA46350.040.843541
ATCAACG54000.034.217013
TCAACGC54850.033.7733544
CAACGCA55400.033.3523185
AACGCAG57100.032.525726
TATCAAC58550.031.7202112
ACGCAGA62350.029.4060757
CGCAGAG62900.028.9979178
GCAGAGT71600.025.1427299
GTACATG33200.024.9287911
TACATGG33200.023.8926662
GAGTACT53050.023.05568912-13
CAGAGTA68050.022.8945410-11
ACATGGG33900.022.8388443
AGAGTAC63050.022.45005410-11
GTACTTT59150.020.43710114-15
CATGGGG20350.019.8399564
GTACCGG1707.8726036E-519.5857031
AGTACTT58200.019.46487412-13
Produced by FastQC (version 0.11.5)