FastQCFastQC Report
Fri 10 Feb 2017
SRR2031830_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031830_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977733
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34770.3556185584408013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.32769682520688165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.1761217019370319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.055.851651
GTATCAA31400.039.3312071
TCAACGC37250.032.5167774
ATCAACG37800.032.043653
CAACGCA38300.031.6253265
AACGCAG39350.031.022876
TATCAAC40400.030.3341582
ACGCAGA43100.027.993047
CGCAGAG43100.027.7726238
GTACATG27800.024.9460451
GCAGAGT49600.023.759
TACATGG28800.023.5850722
ACATGGG28400.023.5827473
GAGTACT31350.023.33333412-13
CAGAGTA46950.022.05537810-11
AGAGTAC43500.021.72988510-11
GTATAGC3700.020.540541
CATGGGG15300.020.1797394
GTACTTT36300.020.08608814-15
AGTACTT34350.020.05094712-13
Produced by FastQC (version 0.11.5)