##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031830_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 977733 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.61226122059908 34.0 31.0 34.0 31.0 34.0 2 32.74362837298117 34.0 33.0 34.0 31.0 34.0 3 32.81829395141619 34.0 33.0 34.0 31.0 34.0 4 35.99385005926976 37.0 37.0 37.0 35.0 37.0 5 36.01706191772191 37.0 37.0 37.0 35.0 37.0 6 36.094056352807975 37.0 37.0 37.0 35.0 37.0 7 36.11523698187542 37.0 37.0 37.0 35.0 37.0 8 36.113176092041485 37.0 37.0 37.0 35.0 37.0 9 37.95375731411336 39.0 39.0 39.0 37.0 39.0 10-11 37.992099581378554 39.0 39.0 39.0 37.0 39.0 12-13 37.97367941963706 39.0 39.0 39.0 37.0 39.0 14-15 39.5585773416669 41.0 40.0 41.0 37.0 41.0 16-17 39.493572376098584 41.0 40.0 41.0 37.0 41.0 18-19 39.504218431821364 41.0 40.0 41.0 37.0 41.0 20-21 39.445484605715464 41.0 40.0 41.0 37.0 41.0 22-23 39.358804499796975 41.0 40.0 41.0 37.0 41.0 24-25 39.329421733745306 41.0 40.0 41.0 37.0 41.0 26-27 39.21140024935232 41.0 40.0 41.0 36.0 41.0 28-29 39.087277917386444 41.0 39.0 41.0 36.0 41.0 30-31 38.93385464129778 41.0 39.0 41.0 35.0 41.0 32-33 38.799875835222906 41.0 39.0 41.0 35.0 41.0 34-35 38.6056939880315 41.0 38.5 41.0 35.0 41.0 36-37 38.492420221062396 40.0 38.0 41.0 35.0 41.0 38-39 38.30773483149285 40.0 38.0 41.0 34.0 41.0 40-41 38.216140295970376 40.0 38.0 41.0 34.0 41.0 42-43 38.04150519620387 40.0 38.0 41.0 33.5 41.0 44-45 38.17854311964514 40.0 38.0 41.0 34.0 41.0 46-47 38.04522553703312 40.0 38.0 41.0 33.0 41.0 48-49 37.86678622896026 40.0 38.0 41.0 33.0 41.0 50-51 36.802784093407915 39.0 36.5 40.0 31.5 40.5 52-53 36.827849218549446 39.5 37.5 40.0 31.5 40.5 54-55 37.591348558348756 40.0 38.0 41.0 33.0 41.0 56-57 37.576125588478654 40.0 37.5 41.0 33.0 41.0 58-59 37.44647823076443 40.0 37.0 41.0 33.0 41.0 60-61 37.23782003880405 40.0 36.5 41.0 32.0 41.0 62-63 36.96875680784018 39.5 36.0 41.0 31.5 41.0 64-65 36.64504010808677 39.0 35.5 41.0 31.0 41.0 66-67 36.37823209403794 39.0 35.0 41.0 31.0 41.0 68-69 36.053087090238336 38.5 35.0 41.0 31.0 41.0 70-71 35.6969418031303 37.0 35.0 40.0 30.0 41.0 72-73 35.29636669724761 37.0 35.0 39.5 30.0 41.0 74-75 34.938895383504494 36.0 35.0 39.0 29.5 41.0 76-77 34.56635656155617 36.0 35.0 39.0 29.0 40.5 78-79 34.19603818220312 35.0 35.0 37.0 29.0 39.0 80-81 33.84243142043891 35.0 35.0 37.0 29.0 39.0 82-83 33.55828124856173 35.0 34.0 36.5 29.0 39.0 84-85 33.25499139335585 35.0 34.0 36.0 28.0 37.0 86-87 32.99009852383013 35.0 34.0 36.0 27.0 37.0 88-89 32.790977189069 35.0 34.0 35.5 26.5 37.0 90-91 32.61939455863717 35.0 34.0 35.0 26.0 36.0 92-93 32.48590412720037 35.0 34.0 35.0 26.0 36.0 94-95 32.31733254375172 35.0 34.0 35.0 25.0 36.0 96-97 32.226891697426595 35.0 34.0 35.0 25.0 36.0 98-99 32.11067182963038 35.0 34.0 35.0 25.0 35.0 100-101 30.613058473018707 34.0 31.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2917059775063393 1101 2 0.21739280084746326 1101 3 0.3381010343626869 1101 4 0.2542551666805437 1101 5 0.25741252841987006 1101 6 0.18883977815565345 1101 7 0.28140876341449683 1101 8 0.3052079340463152 1101 9 0.3224732713085743 1101 10-11 0.34771356008055676 1101 12-13 0.44485513596608683 1101 14-15 0.4106319551140629 1101 16-17 0.5631439216640004 1101 18-19 0.5674830492588256 1101 20-21 0.5721397929973477 1101 22-23 0.511343978660598 1101 24-25 0.4768244162157913 1101 26-27 0.40422823648253825 1101 28-29 0.41960926300514245 1101 30-31 0.4350781421946053 1101 32-33 0.3680196690324635 1101 34-35 0.6544392584674981 1101 36-37 0.48965460763461977 1101 38-39 0.5333436680490635 1101 40-41 0.4062530676685796 1101 42-43 0.46294760099758037 1101 44-45 0.4630854062338372 1101 46-47 0.5593012978796494 1101 48-49 0.5539888040181964 1101 50-51 0.4792840443374118 1101 52-53 0.41381353373205343 1101 54-55 0.6445120771621049 1101 56-57 0.6225343743711704 1101 58-59 0.531679121062048 1101 60-61 0.40851285943940496 1101 62-63 0.4804240914084801 1101 64-65 0.5452787928211364 1101 66-67 0.6461184634510815 1101 68-69 0.6811891060326687 1101 70-71 0.6025676551481567 1101 72-73 0.6232031540973608 1101 74-75 0.6930546595018825 1101 76-77 0.6787566867578718 1101 78-79 0.6679663184294 1101 80-81 0.6117079576146693 1101 82-83 0.6434256965529954 1101 84-85 0.6063006332330119 1101 86-87 0.5230461772972177 1101 88-89 0.6189244265361111 1101 90-91 0.6578862497247329 1101 92-93 0.6038521055539618 1101 94-95 0.5993567502866384 1101 96-97 0.4286294480891044 1101 98-99 0.35407603608496174 1101 100-101 0.273117504130326 1102 1 0.2832749088692168 1102 2 0.28724998988342776 1102 3 0.296005368422648 1102 4 0.29075111774520224 1102 5 0.3570204820705385 1102 6 0.35427007596168636 1102 7 0.39661568597498587 1102 8 0.4197700108144602 1102 9 0.42695320897900757 1102 10-11 0.4908021890787211 1102 12-13 0.40083322115535225 1102 14-15 0.5068142690992588 1102 16-17 0.5518417144347652 1102 18-19 0.5654333016438429 1102 20-21 0.5762517209867966 1102 22-23 0.6503670953047447 1102 24-25 0.6630746130678133 1102 26-27 0.6513724316271237 1102 28-29 0.6860640201933563 1102 30-31 0.5344523599668136 1102 32-33 0.6479158969332275 1102 34-35 0.6941602673444933 1102 36-37 0.7095959250098218 1102 38-39 0.7911327918710569 1102 40-41 0.5425302021661693 1102 42-43 0.6509914306190367 1102 44-45 0.755929109870003 1102 46-47 0.8118951859350787 1102 48-49 0.6144476557285259 1102 50-51 0.5956289788167766 1102 52-53 0.5074976919099043 1102 54-55 0.4801858083989998 1102 56-57 0.5505395795531456 1102 58-59 0.4167598096711558 1102 60-61 0.5092049497178195 1102 62-63 0.477836419732796 1102 64-65 0.44415818676555574 1102 66-67 0.4999639159620912 1102 68-69 0.5984275414942175 1102 70-71 0.580833533854161 1102 72-73 0.5280610476563652 1102 74-75 0.4831341462775711 1102 76-77 0.3606338676711047 1102 78-79 0.2735560870971909 1102 80-81 0.6132819449664026 1102 82-83 0.5096243388956552 1102 84-85 0.6851276438174381 1102 86-87 0.6385838112602045 1102 88-89 0.4944799636727595 1102 90-91 0.500831487563417 1102 92-93 0.5448313096915811 1102 94-95 0.5911196326976054 1102 96-97 0.7064237107850246 1102 98-99 0.7635928990510763 1102 100-101 0.5339493386254333 1103 1 0.07168930095132708 1103 2 0.05070582299200055 1103 3 0.14522064053942785 1103 4 0.19675953625866072 1103 5 0.1532594248167669 1103 6 0.12747326275389526 1103 7 0.0974326878568732 1103 8 0.11221850120954002 1103 9 0.14351780091747912 1103 10-11 0.29614452074633135 1103 12-13 0.2923865247703503 1103 14-15 0.30054023406150776 1103 16-17 0.3647297979047224 1103 18-19 0.2349445248132369 1103 20-21 0.3595810642639563 1103 22-23 0.39340545742204114 1103 24-25 0.3101197996963805 1103 26-27 0.4693213205227522 1103 28-29 0.45945660838621905 1103 30-31 0.49875852960379774 1103 32-33 0.4103989644480066 1103 34-35 0.4630413141736085 1103 36-37 0.4011269550200893 1103 38-39 0.44634496689612746 1103 40-41 0.45083719607804795 1103 42-43 0.3594102471178715 1103 44-45 0.4090525662485831 1103 46-47 0.19130104131726 1103 48-49 0.24638012579553248 1103 50-51 0.37763402750051256 1103 52-53 0.39516144836091627 1103 54-55 0.42739054676747656 1103 56-57 0.4540418387330547 1103 58-59 0.3022393891697419 1103 60-61 0.45551268305276693 1103 62-63 0.5010108175121033 1103 64-65 0.4714297156439642 1103 66-67 0.34906251681606193 1103 68-69 0.4351105017320478 1103 70-71 0.36445531289393784 1103 72-73 0.263877913169253 1103 74-75 0.3381382828041595 1103 76-77 0.2391025649228098 1103 78-79 0.2234775991567588 1103 80-81 0.022388970394679575 1103 82-83 0.1476790198376321 1103 84-85 0.1826207268782838 1103 86-87 0.2536436469506995 1103 88-89 0.2537166911802018 1103 90-91 0.04944855429635453 1103 92-93 -0.08600566267399756 1103 94-95 -0.07872720975376524 1103 96-97 0.06909254319357672 1103 98-99 0.07239218508355805 1103 100-101 0.10217399596042043 1104 1 0.31729996388467185 1104 2 0.28214437801076286 1104 3 0.26174749869212377 1104 4 0.3621629536301043 1104 5 0.3473312378137976 1104 6 0.35597985536603716 1104 7 0.3319925492341511 1104 8 0.36756316457893945 1104 9 0.4144776026227177 1104 10-11 0.2929600997371864 1104 12-13 0.37046795414504885 1104 14-15 0.3990292119484309 1104 16-17 0.4711654840757191 1104 18-19 0.4438224610789163 1104 20-21 0.45427312214582827 1104 22-23 0.42929617727332214 1104 24-25 0.40371308888770585 1104 26-27 0.3406018594071156 1104 28-29 0.44064987692183877 1104 30-31 0.40474638864009904 1104 32-33 0.4990434912602666 1104 34-35 0.5456526541550204 1104 36-37 0.5387914834388994 1104 38-39 0.648370660296635 1104 40-41 0.442533316248344 1104 42-43 0.5174091684311151 1104 44-45 0.6204235312142217 1104 46-47 0.7311077383554618 1104 48-49 0.9072521981363977 1104 50-51 0.7785598046713673 1104 52-53 0.8130076895965885 1104 54-55 0.9142124946003491 1104 56-57 0.860359615261288 1104 58-59 0.6493943528501305 1104 60-61 0.7719422872665334 1104 62-63 0.7415450543412234 1104 64-65 0.8997788085143128 1104 66-67 0.6779959939742994 1104 68-69 0.6705051978063139 1104 70-71 0.6344899111978748 1104 72-73 0.622027735874461 1104 74-75 0.532370751275522 1104 76-77 0.6821487648470779 1104 78-79 0.6393443354407964 1104 80-81 0.7438554106136337 1104 82-83 0.7541606075727358 1104 84-85 0.6958489838811346 1104 86-87 0.7251545543098672 1104 88-89 0.8016078011947769 1104 90-91 0.6735362607099447 1104 92-93 0.6258515719862672 1104 94-95 0.728375770405286 1104 96-97 0.5817484650691114 1104 98-99 0.5666501862999525 1104 100-101 0.6663615956528766 1105 1 0.2118603057939481 1105 2 0.07856977577650781 1105 3 0.17128752915928658 1105 4 0.20879040792935655 1105 5 0.19181118026470756 1105 6 0.19097583539311813 1105 7 0.169220672118378 1105 8 0.2992077566574807 1105 9 0.33103671738236784 1105 10-11 0.2446728197778114 1105 12-13 0.24067836799068232 1105 14-15 0.3544048678387455 1105 16-17 0.31569832090713845 1105 18-19 0.39420224273091975 1105 20-21 0.35830420288381504 1105 22-23 0.38475117473439013 1105 24-25 0.39304958177870475 1105 26-27 0.42785642948158653 1105 28-29 0.48139970765983264 1105 30-31 0.4139843921945996 1105 32-33 0.5272838010097587 1105 34-35 0.5546632118733683 1105 36-37 0.6054436923082278 1105 38-39 0.4810472217387485 1105 40-41 0.5003415846978996 1105 42-43 0.5644931012340919 1105 44-45 0.5193450147958032 1105 46-47 0.656069125457698 1105 48-49 0.5040885852386339 1105 50-51 0.49536064674990854 1105 52-53 0.58398560090329 1105 54-55 0.6401534856294688 1105 56-57 0.6514162334061808 1105 58-59 0.47528720999298457 1105 60-61 0.4529495020600294 1105 62-63 0.45920543010289805 1105 64-65 0.6420562289611524 1105 66-67 0.5672166481719927 1105 68-69 0.6624864097223551 1105 70-71 0.6354525889229947 1105 72-73 0.4271648381087161 1105 74-75 0.4925439274772856 1105 76-77 0.42983728041917857 1105 78-79 0.5536739520623328 1105 80-81 0.5842836435181624 1105 82-83 0.28935388363908743 1105 84-85 0.4851706663455957 1105 86-87 0.5029531959821725 1105 88-89 0.6229755440857758 1105 90-91 0.45551884787987973 1105 92-93 0.5485755267933143 1105 94-95 0.5851272091324304 1105 96-97 0.5957363839926018 1105 98-99 0.7337117976743599 1105 100-101 0.9210110116989156 1106 1 0.13084007806193654 1106 2 0.1031828548220517 1106 3 0.1885301647880766 1106 4 0.1352098467603895 1106 5 0.1570125831871394 1106 6 0.11725318460810286 1106 7 0.12264563908557591 1106 8 0.11418400218833824 1106 9 0.21867054879849945 1106 10-11 0.1140050228712326 1106 12-13 0.17775513524546227 1106 14-15 0.2047596061023924 1106 16-17 0.19176702913036792 1106 18-19 0.18454233483413418 1106 20-21 0.244333150303369 1106 22-23 0.2009036065313765 1106 24-25 0.12672749255128224 1106 26-27 0.15016717097078214 1106 28-29 0.13998629245696748 1106 30-31 -0.0030067326693838936 1106 32-33 0.12191427085275564 1106 34-35 0.0314024130625441 1106 36-37 0.10648689726703253 1106 38-39 0.21698338585346733 1106 40-41 0.27333217035000956 1106 42-43 0.08572213997962308 1106 44-45 -0.005925684139377552 1106 46-47 0.07668414275133983 1106 48-49 -0.13019642467121173 1106 50-51 -0.06391076141891006 1106 52-53 -0.1070095607631174 1106 54-55 -0.20623124447313046 1106 56-57 -0.3370538621946295 1106 58-59 -0.12576886804214382 1106 60-61 -0.13569802339466008 1106 62-63 -0.12188766409887108 1106 64-65 -0.09035380523750547 1106 66-67 -0.10809825642150628 1106 68-69 7.374201374048539E-4 1106 70-71 0.04049543440721237 1106 72-73 0.051261293883634096 1106 74-75 0.07506216838355328 1106 76-77 -0.12200356949532676 1106 78-79 -0.01033863440588334 1106 80-81 0.06363814375133359 1106 82-83 -0.1094874180475287 1106 84-85 -0.01494834888835328 1106 86-87 0.012550317270843436 1106 88-89 -0.06963786043000653 1106 90-91 0.011931461551576206 1106 92-93 -0.031864805283476016 1106 94-95 0.004160416811586742 1106 96-97 0.0026488616468896 1106 98-99 0.025197107794056706 1106 100-101 -0.10876101340600997 1107 1 -0.13308233602369768 1107 2 -0.04784793657870523 1107 3 0.018021454018210648 1107 4 0.06507010795905899 1107 5 0.0667694145979425 1107 6 0.019070671300454478 1107 7 0.03562390570910878 1107 8 -0.0249100596478371 1107 9 -0.003522515614363897 1107 10-11 -0.010480599438110971 1107 12-13 -0.04496050749193614 1107 14-15 -0.06582850942587726 1107 16-17 0.004145009353827334 1107 18-19 -0.06657747586130114 1107 20-21 0.0457171910780545 1107 22-23 0.045847152236611066 1107 24-25 0.03442172315084946 1107 26-27 0.04728767391283384 1107 28-29 0.07029560905572652 1107 30-31 0.10680371392017207 1107 32-33 0.0651675810810417 1107 34-35 0.09150401043291367 1107 36-37 0.05628318221022255 1107 38-39 0.22419881254658947 1107 40-41 0.20238763830645468 1107 42-43 0.21929406462881218 1107 44-45 0.17995836681540567 1107 46-47 0.30455883254115435 1107 48-49 0.40585921747801024 1107 50-51 0.25277057212858267 1107 52-53 0.23034641913911003 1107 54-55 0.10516661861134935 1107 56-57 0.10625826637321723 1107 58-59 0.001525329981106438 1107 60-61 0.031688225330007924 1107 62-63 0.04735419012666142 1107 64-65 0.09805377870871013 1107 66-67 0.2528105095471105 1107 68-69 0.2844143129002603 1107 70-71 0.2677615806069866 1107 72-73 0.23840258684646898 1107 74-75 0.1452403653299683 1107 76-77 -0.058043715274315844 1107 78-79 0.07741889377602007 1107 80-81 0.06551693725145924 1107 82-83 0.0014202167991683723 1107 84-85 0.07765377445370092 1107 86-87 0.22545630520403392 1107 88-89 0.2033663315078087 1107 90-91 0.16848778350507132 1107 92-93 0.15659198246601846 1107 94-95 0.2234504568306832 1107 96-97 0.2328542281417043 1107 98-99 0.27970971867227945 1107 100-101 0.14512325924866332 1108 1 0.06352882687959749 1108 2 0.11642614342571278 1108 3 0.025673838075086053 1108 4 0.12638580410621358 1108 5 0.1806048655120449 1108 6 0.1507687636727013 1108 7 0.056301375824780564 1108 8 0.11103628978165858 1108 9 0.0948616389495669 1108 10-11 0.15227829365786505 1108 12-13 0.07548782643954155 1108 14-15 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-0.45297667855859913 2316 90-91 -0.3818790434575021 2316 92-93 -0.6531840666717308 2316 94-95 -0.6173328972505452 2316 96-97 -0.6975410666152939 2316 98-99 -0.6177319713226019 2316 100-101 -0.3634447253831503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 16.0 7 398.0 8 887.0 9 667.0 10 702.0 11 885.0 12 1137.0 13 1123.0 14 1156.0 15 1332.0 16 1539.0 17 1992.0 18 2714.0 19 3664.0 20 4609.0 21 6321.0 22 7239.0 23 7175.0 24 6536.0 25 5906.0 26 5761.0 27 5945.0 28 6383.0 29 7515.0 30 8956.0 31 10842.0 32 13830.0 33 18721.0 34 27799.0 35 46294.0 36 88240.0 37 209143.0 38 341935.0 39 130205.0 40 166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.010310585814324 19.57589648707776 13.829542421090421 23.584250506017494 2 15.343861770033332 21.492677448751348 39.77578745935752 23.387673321857807 3 17.529427768112562 26.369264410631533 31.744351474277742 24.356956346978162 4 12.37393030612652 18.368102539241285 39.10597269397678 30.151994460655413 5 12.953536394905358 38.03042343870975 35.75516015108419 13.2608800153007 6 29.54201198077594 38.25983167183679 18.5618159558898 13.636340391497473 7 25.21455243916284 33.878778766800345 23.56942028140607 17.337248512630747 8 24.716052337396814 34.73453386558498 21.900355209448797 18.64905858756941 9 25.985212731901246 15.402671281423455 21.518962743407457 37.09315324326784 10-11 23.419583874125145 26.317460901902667 30.444047608089324 19.818907615882864 12-13 24.61433745204468 24.979263254896786 29.35867972135542 21.047719571703112 14-15 22.07964751113034 26.359548056575772 27.32668325606275 24.234121176231138 16-17 21.181038177089246 28.796665347288062 28.150425525168938 21.871870950453754 18-19 21.113688501871163 28.056943971411417 30.063064251692435 20.76630327502498 20-21 22.16873113620999 27.45805859063773 29.77321006859746 20.60000020455482 22-23 21.742812432880243 27.311681138964744 29.587820768514828 21.35768565964019 24-25 21.448855282718498 27.66426944848299 29.663133143540065 21.223742125258443 26-27 21.5540438954193 27.598383198685124 29.573717978221048 21.273854927674527 28-29 21.48597827832343 27.53088010735037 29.677734105323232 21.30540750900297 30-31 21.782071383496312 27.43688716653729 29.587423151310226 21.193618298656176 32-33 21.457647435445054 27.739423748610303 29.49946457775282 21.303464238191815 34-35 21.752104102040125 27.7119622637264 29.345434796616253 21.190498837617223 36-37 21.836738659736348 27.553790247439743 29.377396487589145 21.232074605234764 38-39 21.714005766400437 27.641799959702702 29.33510477809381 21.309089495803047 40-41 21.83914217889751 27.59521259894061 29.36036729863879 21.205277923523088 42-43 21.77695751294065 27.662664551569804 29.201325924357675 21.359052011131872 44-45 21.742285470573254 27.874736763513148 28.89761315205685 21.485364613856746 46-47 21.80820326203575 27.845792256168096 28.70343948705833 21.64256499473783 48-49 21.824823341341652 27.832751886251156 28.654704300662857 21.68772047174433 50-51 21.72658588796737 28.093150174945514 28.498680110009584 21.681583827077535 52-53 21.88532043001515 28.002021001643595 28.346184490039718 21.76647407830154 54-55 21.85525087114785 27.946740060936882 28.421409525913514 21.776599542001755 56-57 21.71170452465039 28.109156589784735 28.375026719973658 21.80411216559122 58-59 21.84338669145871 28.050193662277945 28.382799803218262 21.723619843045086 60-61 21.814237629291434 28.0514209912113 28.37293003304583 21.761411346451435 62-63 21.740444477173217 28.0074417044326 28.4725993701757 21.77951444821848 64-65 21.71825027896164 27.979264277670897 28.412153420207765 21.890332023159697 66-67 21.486029417028984 28.097343548801156 28.470144712308986 21.946482321860877 68-69 21.611319245642726 27.91989224051965 28.422841409669104 22.04594710416852 70-71 21.607534981431538 27.678108440647907 28.4899353913594 22.22442118656116 72-73 21.584266870403273 27.85417900387938 28.35809980843441 22.20345431728294 74-75 21.52208220444641 27.96965020102625 28.29064785580521 22.217619738722124 76-77 21.52341181079088 27.891203426702383 28.37968034217931 22.20570442032743 78-79 21.44286834953919 27.87177071859086 28.222121990359334 22.463238941510618 80-81 21.594648027631266 27.76724320443311 28.29366503943306 22.344443728502565 82-83 21.56074306584722 27.803193714439423 28.332377039539423 22.303686180173933 84-85 21.658673686988166 27.709047357509668 28.162801091913643 22.469477863588523 86-87 21.392241031038125 27.674324176436716 28.404687169196496 22.52874762332866 88-89 21.59076148600896 27.673403679736698 28.296426529533115 22.43940830472123 90-91 21.589789850603385 27.60073557914073 28.291926323444134 22.517548246811756 92-93 21.654378035721408 27.3498490896799 28.508089631832 22.487683242766686 94-95 21.441283049666936 27.278357179311737 28.587303486739224 22.693056284282108 96-97 21.550310769913665 27.220519303327183 28.479912205070303 22.749257721688846 98-99 21.590505792481178 27.178585564770753 28.36260001452339 22.86830862822468 100-101 21.681686104488648 27.09502491989122 28.544040141838313 22.67924883378182 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 737.0 1 476.5 2 250.5 3 283.0 4 245.5 5 251.5 6 338.5 7 602.5 8 1015.5 9 1265.0 10 1116.5 11 933.0 12 970.0 13 1021.0 14 1137.0 15 1232.0 16 1285.0 17 1408.5 18 1527.0 19 1660.5 20 1951.5 21 2351.5 22 2778.5 23 3342.0 24 4160.5 25 5053.5 26 6298.5 27 8015.0 28 9740.0 29 11517.5 30 13645.0 31 16237.0 32 18753.0 33 20683.0 34 22552.5 35 24325.0 36 26387.5 37 28560.0 38 30062.0 39 31423.5 40 32709.0 41 33450.0 42 34014.0 43 34874.5 44 35606.5 45 36443.0 46 36879.0 47 36833.5 48 37025.5 49 36544.5 50 35095.5 51 33354.5 52 31731.5 53 30319.0 54 28257.5 55 25679.0 56 23390.0 57 21434.5 58 20079.5 59 17107.5 60 12937.5 61 9965.5 62 7670.0 63 5683.5 64 3977.0 65 2869.0 66 2144.5 67 1558.0 68 1131.5 69 829.5 70 655.0 71 529.5 72 401.5 73 319.0 74 261.5 75 207.5 76 158.5 77 102.0 78 65.0 79 46.5 80 32.5 81 29.0 82 27.0 83 18.5 84 13.0 85 12.0 86 9.5 87 5.5 88 3.0 89 3.5 90 3.5 91 3.0 92 1.5 93 0.5 94 2.5 95 2.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.0683223333977685E-4 24-25 2.5569352778314735E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 977733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.80387018678769 #Duplication Level Percentage of deduplicated Percentage of total 1 71.74031017856028 44.33828817435624 2 16.336306529144387 20.192939361176236 3 5.895143915833698 10.930281279198512 4 2.6560199896771226 6.566092586220723 5 1.2746042748507762 3.9387738571201014 6 0.7143246518303231 2.6488816831766164 7 0.41656236244678746 1.8021616321353997 8 0.2621242037337496 1.29602322083006 9 0.1748871089916249 0.9727830163314879 >10 0.5055892742462288 4.677924559862994 >50 0.014146204185538951 0.6179227301475586 >100 0.00914953121523952 1.0352329932062228 >500 3.3271011370225654E-4 0.11875001221016955 >1k 4.990651705533848E-4 0.863944894027753 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3477 0.3556185584408013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3204 0.32769682520688165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1722 0.1761217019370319 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0227741111325894E-4 0.0 5 0.0 0.0 0.0 1.0227741111325894E-4 0.0 6 0.0 0.0 0.0 1.0227741111325894E-4 0.0 7 0.0 0.0 0.0 1.0227741111325894E-4 0.0 8 0.0 0.0 0.0 1.0227741111325894E-4 0.0 9 0.0 0.0 0.0 1.0227741111325894E-4 0.0 10-11 0.0 0.0 0.0 1.534161166698884E-4 0.0 12-13 0.0 0.0 0.0 3.068322333397768E-4 0.0 14-15 0.0 0.0 0.0 3.068322333397768E-4 0.0 16-17 0.0 0.0 0.0 3.5797093889640625E-4 0.0 18-19 0.0 0.0 0.0 4.0910964445303575E-4 0.0 20-21 0.0 0.0 0.0 5.625257611229242E-4 0.0 22-23 0.0 0.0 0.0 0.0014830224611422547 0.0 24-25 0.0 0.0 0.0 0.0028126288056146208 0.0 26-27 0.0 0.0 0.0 0.005932089844569019 0.0 28-29 0.0 0.0 0.0 0.01636438577812143 0.0 30-31 0.0 0.0 0.0 0.03779150340634918 0.0 32-33 0.0 0.0 0.0 0.06852586544588349 0.0 34-35 0.0 0.0 0.0 0.10994821694675336 0.0 36-37 0.0 0.0 0.0 0.17325793442586063 0.0 38-39 0.0 0.0 0.0 0.2576367985942993 0.0 40-41 0.0 0.0 0.0 0.35030013306291186 0.0 42-43 0.0 0.0 0.0 0.4344744424091239 0.0 44-45 0.0 0.0 0.0 0.5404338403224602 0.0 46-47 0.0 0.0 0.0 0.6708886781974219 2.0455482222651788E-4 48-49 0.0 0.0 0.0 0.806968773683613 2.0455482222651788E-4 50-51 0.0 0.0 0.0 0.9427931756420209 2.0455482222651788E-4 52-53 0.0 0.0 0.0 1.0731457361058694 2.5569352778314735E-4 54-55 0.0 0.0 0.0 1.224465165847936 3.068322333397768E-4 56-57 0.0 0.0 0.0 1.4016607806016572 3.068322333397768E-4 58-59 0.0 0.0 0.0 1.6085168445782232 3.068322333397768E-4 60-61 0.0 0.0 0.0 1.8221232176882647 3.068322333397768E-4 62-63 0.0 0.0 0.0 2.0183935696146085 3.068322333397768E-4 64-65 0.0 0.0 0.0 2.233892074830245 3.068322333397768E-4 66-67 0.0 0.0 0.0 2.5041089949914754 3.068322333397768E-4 68-69 0.0 0.0 0.0 2.8232656563704 3.068322333397768E-4 70-71 0.0 0.0 0.0 3.1553604102551516 4.0910964445303575E-4 72-73 0.0 0.0 0.0 3.501416030756863 4.0910964445303575E-4 74-75 0.0 0.0 0.0 3.86363148221447 4.0910964445303575E-4 76-77 0.0 0.0 0.0 4.250086680105918 4.0910964445303575E-4 78-79 0.0 0.0 0.0 4.681134829242748 4.0910964445303575E-4 80-81 0.0 0.0 0.0 5.157594148913865 4.6024835000966526E-4 82-83 0.0 0.0 0.0 5.66018534712442 5.113870555662947E-4 84-85 0.0 0.0 0.0 6.1787318214686415 5.113870555662947E-4 86-87 0.0 0.0 0.0 6.768105403008797 5.113870555662947E-4 88-89 0.0 0.0 0.0 7.418692015100237 5.113870555662947E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1820 0.0 55.85165 1 GTATCAA 3140 0.0 39.331207 1 TCAACGC 3725 0.0 32.516777 4 ATCAACG 3780 0.0 32.04365 3 CAACGCA 3830 0.0 31.625326 5 AACGCAG 3935 0.0 31.02287 6 TATCAAC 4040 0.0 30.334158 2 ACGCAGA 4310 0.0 27.99304 7 CGCAGAG 4310 0.0 27.772623 8 GTACATG 2780 0.0 24.946045 1 GCAGAGT 4960 0.0 23.75 9 TACATGG 2880 0.0 23.585072 2 ACATGGG 2840 0.0 23.582747 3 GAGTACT 3135 0.0 23.333334 12-13 CAGAGTA 4695 0.0 22.055378 10-11 AGAGTAC 4350 0.0 21.729885 10-11 GTATAGC 370 0.0 20.54054 1 CATGGGG 1530 0.0 20.179739 4 GTACTTT 3630 0.0 20.086088 14-15 AGTACTT 3435 0.0 20.050947 12-13 >>END_MODULE