FastQCFastQC Report
Fri 10 Feb 2017
SRR2031830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977733
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.41851916627545555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37160.3800628596968702No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT29810.3048889625286249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.20455482222651789No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.051.7888951
GTATCAA30950.040.2772751
ATCAACG38000.032.4994243
TCAACGC38250.032.1628274
CAACGCA39100.031.4636335
TATCAAC40100.031.1528152
AACGCAG40000.030.9931986
ACGCAGA44350.027.8461787
CGCAGAG44800.027.24848
GTACATG25800.024.711811
GCAGAGT50300.024.080099
ACATGGG26450.022.9863593
GTACCGT1252.2558402E-422.7995936
TACATGG26550.022.7208772
GAGTACT34850.022.28439712-13
GTATTAC2154.3422915E-822.1299781
AGAGTAC45250.021.83386810-11
CATGGGG14500.021.6203064
CAGAGTA49350.021.03052710-11
TGTACCG1150.0037232720.6518065
Produced by FastQC (version 0.11.5)