FastQCFastQC Report
Fri 10 Feb 2017
SRR2031829_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031829_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468779
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74640.5081772002459186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63690.43362548075646506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.24367178452306304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31050.062.7214161
GTATCAA58200.042.7663231
ATCAACG69850.035.3614923
TCAACGC70500.035.035464
CAACGCA71950.034.4614335
AACGCAG73000.034.095896
TATCAAC73500.033.7346952
ACGCAGA80650.030.7439547
CGCAGAG82100.029.969558
GTACATG45250.028.0276241
CGTATAC700.00839231627.1428593
GCAGAGT90100.026.7286349
TACATGG46700.026.648822
GAGTACT63250.025.38339812-13
ACATGGG48300.025.077643
CAGAGTA88000.023.77698910-11
GTACTTT68800.023.05959314-15
AGAGTAC81850.021.82040410-11
AGTACTT66400.021.1389312-13
TAAGGCG1604.9904993E-520.781255
Produced by FastQC (version 0.11.5)