FastQCFastQC Report
Fri 10 Feb 2017
SRR2031829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468779
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89590.6099624245717021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80270.5465083583030531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44700.3043344165459882No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT35570.24217394175706486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.065.23441
GTATCAA65100.044.7845541
ATCAACG81700.035.5808223
TCAACGC82400.035.2785574
CAACGCA83050.034.9452555
TATCAAC83550.034.679272
AACGCAG84050.034.5294846
ACGCAGA91700.031.5452947
CGCAGAG92850.031.0011168
GCAGAGT102400.027.6460389
GAGTACT76500.025.33292612-13
CAGAGTA100650.024.18614810-11
AGAGTAC93600.023.49588410-11
GTACTTT82800.023.34805314-15
GTACATG44300.022.6530951
TCTATAC5050.022.5738933
TACATGG43850.022.314352
AGTACTT78550.021.98086712-13
ACATGGG45900.020.593353
ACTTTTT99100.019.6515516-17
Produced by FastQC (version 0.11.5)