FastQCFastQC Report
Fri 10 Feb 2017
SRR2031828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119389
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33950.3032904557754275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.27818747548886047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17420.15562061088683202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.057.455091
GTATCAA31200.038.8221131
ATCAACG35450.033.6318743
TCAACGC35400.033.411024
CAACGCA36000.032.8541685
AACGCAG37450.032.0894556
TATCAAC38550.031.4202332
ACGCAGA41000.029.1951247
CGCAGAG40800.028.988978
GCGGAAC700.00839147827.1428593
GACCGTC1151.281724E-424.7826087
GCAGAGT47800.024.147499
GAGTACT34600.023.20086712-13
GGACCGT1050.002196185522.619056
TACATGG23450.022.281452
TCGCCGG753.0348707E-522.16666894-95
GTACATG23250.021.8602161
CAGAGTA46550.021.83673510-11
AGAGTAC43000.021.43023310-11
TAGGCCG1353.795859E-421.1111135
Produced by FastQC (version 0.11.5)